Team:Mississippi State/17 July 2008

From 2008.igem.org

  1. Extract plasmid using P1,P2,P3 buffers
  2. Prepare and run 1% agarose gel
  3. Check O.D. for samples: BKM#1 and BKM#6
  4. Make sequencing mix(x2): 4ul TRR, 1ul Template, 1ul Sp6(#1) 1ul T7(#2), 2ul 5xBuffer, 12ul ddH2O
  5. Run PCR: 96C, 2min; 96C, 10sec; 50C 5sec; 60C 4min;
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