Team:Mississippi State/Running a Gel

From 2008.igem.org

Running a Gel

  • Electrophoresis passes electricity through a sample of DNA or proteins and separates them by size. Therefore, you can see how much of certain size units are present in the sample.
  1. WEAR GLOVES!!!
  2. Weigh 0.25g Agarose into flask with 25ml 1xTAE Buffer, stirring
  3. Weigh, remember weight
  4. Microwave to boil several times
  5. allow to cool until just cool enough to hold in hand.
  6. Reweigh, adding ddH2O until original weight
  7. Add 5ul EtBr
  8. Pour in gel tray
  9. allow gel to set, with template in place
  10. remove template
  11. pour 1xTAE until full
  12. on parafilm, put 1ul drop of dye down for every sample.
  13. load 1ul DNA Ladder into first well
  14. Get 2ul of sample, mix with dye on parafilm
  15. Pipette mix into well
  16. Hook up Electrophoresis: Red(-) on right, black(+) on left, 80V voltage, for 60min
  17. When gel is done, turn off power, remove gel, wrap in Saran Wrap, take picture next door.
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