Team:Montreal/Notebook/July

From 2008.igem.org

Home The Team The Project Parts Submitted to the Registry Modeling Notebook

July 2008
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Contents

July 2008

July 1

  • Maxi-prep of J-brick, assuming high copy number plasmid.

July 2

  • Gel of J-brick maxi-prep, results indicate large plasmid at well over 13K. Assumed incorrect, to be repeated.

July 3

  • Repeat gel, of J-brick maxi prep from July 1st, negative results. Gel from July 2nd assumed contamination from other lanes.

July 4

  • Preparation of media.

July 7

  • Seeding of J-brick for mini-prep, 4x5mL cultures with ampicillin.

July 8

  • Mini-prep of J-brick cultures followed by gel giving negative results - no DNA seen, but ladder appears normal.

July 9