Team:NTU-Singapore/Notebook/2 July 2008

From 2008.igem.org

Wednesday 2 July

  • Arrival of E7+Imm Oligo
    • PCR E7+Imm
  • Attempted Ligation of Fe-GFP & LacI-Rbs34
    • Failed Attempt due to faulty gel
      • Loading dye was unable to keep DNA sample settled at the bottom of the well
      • Samples settled into the gel and started floating out of it.
  • Preliminary Speculation of problem is due to loading dye.

Luchao, Hung

    • miniprep for pFe,pLacI,GFP and RBS 34
    • Transformation and cloning for BBa_I763004 LacI-GFP and BBa_J04450 LacI-RFP. (the transformation on Tuesday was uncessful possibly because of the old agar plates).