Hung, Darius

• • Ligation of E7 into empty plasmid vector extracted from GFP plasmid.
    • Gel electrophoresis for the E7 plasmids synthesized by PCR (with 2 blunt ends recognized by EcoRI and PstI).
    • Gel extraction of E7 bands (around 2kb) to remove all the oligonucleotides.
    • Purification for E7 after extracted.
    • Digestion of GFP by EcoRI and PstI. Incubation for 3 hours.
    • Digestion of purified E7 by EcoRI and PstI. Incubation for 3 hours.
    • Gel electrophoresis and gel extraction for GFP to get the empty plasmid vector (around 2kb).
    • PCR purification for digested E7 to remove all the enzymes, buffers and small nucleotides.
    • Ligation of empty plasmid (from GFP) and E7 insert.
    • Transformation and cloning into homemade top10 cells.

Lu Chao, Hung

transformation and cell cloning of LacI-GFP and LacI-RFP again.

Lu Chao

inoculation of LacI-RBS cells.

Choon Kit

documentation of lab protocols.