Team:NYMU-Taipei/Project/Phosphate/Experiments/Assembly

From 2008.igem.org

Contents

Auto-Regulated Phosphate Absorption Device

Phosphate Absorption Circuit

Phosphate Regulatory Circuit

Operations of Design

Testing Circuits

Test of pTet and tetR regulatory pair

  • RBS (B0034) + tetR (C0040) + D-term (B0015)
    • S03880 (failed;9/19, 2008)
    • P0440 (succeed; 9/19, 2008)
  • plasmid extraction (9/20)
    • pLac: R0010 (54.2 ng/uL)
    • pTet: R0040 (26.4 ng/uL)
    • RBS: B0032 (80.9 ng/uL)
    • D-term: B0015 (118.4 ng/uL)
  • plasmid extraction (9/23)
    • P0440 (114.62 ng/uL)
  • vector digestion (9/23)
    • pLac: R0010 D. SP (10.87 ng/uL)
    • pTet: R0040 D. SP (5.55 ng/uL)
    • RBS: B0032 D. SP (15.32 ng/uL)

Test of PPK and PST for pi absorption

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