Method & Algorithm : ƒ4
= act_pFliA
Specific Plasmid Characterisation for ƒ4
According to the characterization plasmid (see right) and to our modeling, in the exponential phase of growth, at the steady state,
we have [FlhDC]_{real} = {coef_{flhDC}} ƒ1([aTc]_{i})
and [FliA]_{real} = {coef_{fliA}} ƒ2([arab]_{i})
but we use [aTc]_{i} = Inv_ƒ1( [FlhDC] )
and [arab]_{i} = Inv_ƒ2( [FliA] )
So, at steadystates,
we use this analytical expression to determine the parameters :
↓ Table of Values ↑
param
 signification
 unit
 value
 comments

(fluorescence)
 value of the observed fluorescence
 au

 need for 20 mesures with well choosen values of [aTc]_{i} and for 20 mesures with well choosen values of [arab]_{i} and 5x5 measures for the relation below?

conversion
 conversion ratio between fluorescence and concentration ↓ gives ↓
 nM.au^{1}
 (1/79.429)


[GFP]
 GFP concentration at steadystate
 nM



γ_{GFP}
 dilutiondegradation rate of GFP(mut3b) ↓ gives ↓
 min^{1}
 0.0198
 Time Cell Division : 35 min.

ƒ4
 activity of pFliA with RBS E0032
 nM.min^{1}



param
 signification corresponding parameters in the equations
 unit
 value
 comments

β_{18}
 total transcription rate of FlhDC><pFliA with RBS E0032 β_{18}
 nM.min^{1}



(K_{1}/{coef_{flhDC}})
 activation constant of FlhDC><pFliA K_{1}
 nM



n_{1}
 complexation order of FlhDC><pFliA n_{1}
 no dimension



β_{23}
 total transcription rate of FliA><pFliA with RBS E0032 β_{23}
 nM.min^{1}



(K_{7}/{coef_{fliA}})
 activation constant of FliA><pFliA K_{7}
 nM



n_{10}
 complexation order of FliA><pFliA n_{7}
 no dimension




Then, if we have time, we want to verify the expected relation
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