Sunday, July 13th

Taylor Stevenson
1. Innoculated 100mL of LB with 1.0mL of O/N cultures and grew at 37*C (for all strains excluding the VCS257 containing the temperature sensitive phage. All 5.0mL of this O/N were used to innoculate the 100mL of LB. The Resulting culture was grown at 30*C).
2. All strains were grown to OD600nm of approximately 0.8 (Note: the 7524 strain never reached an appropriate optical density, however, I continued the prep with the slightly reduced titer).
3. Cells were made electrocompetent following the procedure outlined here.
4. Procedure resulted in 10x40uL stocks of each of the 5 strains.

Home	The Team	The Project	Parts Submitted to the Registry	Modeling	Notebook

Team:Rice University/Notebook/13 July 2008