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Team:Rice University/Notebook/21 May 2008
From 2008.igem.org
Wednesday 21 May
- Taylor Stevenson
- Transformation From New Registry (Paper Punches)-Electroporation of BBa_I13602 located on pSB1A2 into TetR electrocompetent cells.
- Added 2uL of DNA solution obtained by soaking DNA punch in (10:1) Tris:EDTA buffer incubated @ 42*C for 20min.
- Electropulse parameters: 1.8 kV & 5.4 ms
- Plated 100uL and 20uL of transformed cells as well as 20uL of 1/100 and 1/10,000 dilutions in sterile 50% glycerol. Plates were cultured O/N.
- Result-no colonies were observed at any dilution
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