From 2008.igem.org
- David Ouyang
- Not1 Digest of bleoC and STF
- Gel Purify
- DCC
Left lane: STF
Right lane: BleoC, undigested/digested?
I think there is something wrong with the Not1 - very incomplete digestion, and I let it run for at least 2 hours.
- Overnight the ARFP plasmid mutants