Team:Rice University/Notebook/7 July 2008
From 2008.igem.org
- David Ouyang
- Ran a gel of:
- GFP overnights (over long weekend) were miniprepped
- PCR product of SupF
- Ran a gel of:
- Did quikchange of GFP to create Amber Suppressed GFP.
- PCR
- 5uL Buffer
- 1uL RFP (60ish ng/mL)
- 1uL dNTP
- 2.5uL QC-RFP-F
- 2.5uL QC-RFP-R
- 1uL PfuTurbo
- 37uL dH2O
- PCR
- Did quikchange of GFP to create Amber Suppressed GFP.
First lane: 1kb ladder
Second lane: [ptet][RFP] plasmid, supercoiled? Should be around 6 or 7. Faint band around there.
Third lane: 100bp ladder
Fourth and Fifth lanes: +- DMSO, Colony PCR of SupF. Theoretical length (170). Should be around where the bottom band is. Second band could be artifact from genomic.