Team:Rice University/Notebook/7 July 2008

From 2008.igem.org

  • David Ouyang
    • Ran a gel of:
      • GFP overnights (over long weekend) were miniprepped
      • PCR product of SupF
    • Did quikchange of GFP to create Amber Suppressed GFP.
      • PCR
        • 5uL Buffer
        • 1uL RFP (60ish ng/mL)
        • 1uL dNTP
        • 2.5uL QC-RFP-F
        • 2.5uL QC-RFP-R
        • 1uL PfuTurbo
        • 37uL dH2O


      SupF and RFP.jpg

First lane: 1kb ladder

Second lane: [ptet][RFP] plasmid, supercoiled? Should be around 6 or 7. Faint band around there.

Third lane: 100bp ladder

Fourth and Fifth lanes: +- DMSO, Colony PCR of SupF. Theoretical length (170). Should be around where the bottom band is. Second band could be artifact from genomic.