Team:The University of Alberta/18 July 2008
From 2008.igem.org
Today
- Got the sequencing back that Winnie did on the BisDA gene: it was LLC because she used the primers; no big deal though because the size was correct and we're pretty sure that it's BisDA
- Began westerns on the total/soluable crude butanol proteins
- Digested Tryp and J6 with Xba and Pst.
- Ligated them together, left the reaction to go overnight. Will transform tomorrow.
- Did mni-preps of all biobricks: ER01, ER02, BisDA, BisDB, Lac1 ERE, TDNA-MCS, RBS-6x His, TetR-RBS-6x His.
- Double digested the above parts with Xbal/Pst. Gel didnot turn out good- Possibly due to water contamination, and also only one enzyme worked in each case. Redoing digestions on Monday.
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