Team:The University of Alberta/29 May 2008

From 2008.igem.org

Volunteers Scheduled for Today

130-4 NK
4-8 AH
5-9 KR
6-9 DL

On this Episode of "What we Did in the Lab Today"

Chris:

  • Got sequencing results on B0043 back. I aligned them with the sequences from last year to see what they were (just doing a Blast on the optimized sequences doesnt seem to work for some reason):
    • B0043-VF (the first gene) seems to be ß-Hydroxy Butyryl Co-A Dehydrogenase (1)
    • B0043+1 (the second gene) seems to be Enoyl-CoA Hydratase
    • B0043+2 (the third gene) seems to be Butyryl-CoA Dehydrogenase
    • B0043+3 (the fourth gene) seems to be Butyraldehyde Dehydrogenase
    • B0043+4 (the fourth gene) seems to be Butanol Dehydrogenase
    • B0043+5 (the fifth gene) aligned with nothing, which is good because there was no 5th gene in the operon
    • B0043-VR (sequencing from reverse) aligned with part of Butanol Dehydrogenase, which confirms that it is the last gene in the operon so far.

I dunno if these are in the correct order but I don't think it really matters. As long as the enzymes are being produced and have the expected activity, things should be fine (correct me if I'm wrong!)

Jason

  • Researched the GAA promoter that we could used for our ERE.
    • Designed a Possible BioBrick
  • Learned how to run a protien gel

Tune in Later for the Exciting conclusion of "What we did in the lab today"