"
Team:The University of Alberta/3 July 2008
From 2008.igem.org
Today
Our primers finally arrived! We're still waiting on the site-directed mutageneis kit though (it's on back order) :(
Chris
- After staining the gels from yesterday, there were no apparent bands. Could the Ni-NTA column purifications not have worked? Will run two more gels, with the crude and uninduced purified samples as well as induced pure.
- Ran the gels in the following order:
- Gel 1: The I0500 parts (crude uninduced, purified uninduced, purified induced)
- Gel 2: The J61003 parts (crude uninduced, pure (un)induced)
- Ran the gels in the following order:
Jason
- Gel purified PCR products from the colony PCR yesterday
- Sequenced the purified products - won't get the results back until Monday.
Saima
- Troubleshooting: tried to determine the source of the strange bands from yesterday's colony PCR (esp. the bands in the water controls)
Tom
- Made two SDS-PAGE gels for Chris to use (see above)
