Team:The University of Alberta/3 October 2008

From 2008.igem.org

  • James
    • transformed tom's ligation from yesterday,incubate overnight in the 37degree
    • ligate the digest (21+23)(x/p) and (24+25) (s/p) form 9/10/08 to to vector and incubate at 37 overnight

David

i miniprepped yesterdays cultures and checked them with a NotI digest.

 pTET+BisdA worked
 pTET+BisdB worked