Team:University of Ottawa/18 July 2008

From 2008.igem.org

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Contents

Today in the Lab

Matt

PCR Amplification
  • A PCR Amplification was performed on 600/1 plasmids with integration primers so that yeast integration can be performed next week. Corey suggested it is better to make new stock of this PCR amplification product for integration.
  • Transformation
  • The transformation was a success with both plates streaked growing numerous colonies.
  • For miniprep on Monday, I think I will choose 5 colonies per plate.
  • An inoculation will be performed today into liquid media so that a miniprep can be performed.
  • Dan

    PCR
  • PCR amplification of 0B and 1 A was performed today, absorbance measurements indicated decent concentrations
  • Digestion
  • 1A 0B and T were pooled and digested with PstI and SphI as part of our new strategy for designing the constructs. It should help eliminate virtually all possible byproducts.
  • Ligation
  • Was performed overnight.
  • Tammy

    Gel Electrophoresis of pDR197::AtCKX2 Digestion Products
  • Total plasmid size = 7.9 kb
  • AtCKX2 insert size = 1.67 kb (NM_127508)
  • pDR197 vector only = 6.23 kb
  • Glycerol Stock confirmed pDR197::AtCKX2
  • Stocked PURE I and 1:10 II
  • 0.5 mL Sample
  • 0.5 ml LB + Glucose