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Team:University of Ottawa/27 June 2008
From 2008.igem.org
Contents |
Today in the lab
Dan
- Ligation
- Ligation was performed on the digested 0 1 A B PCR products, new samples were created according to the following legend:
Components New Name 0B + 0A 1 0B + 1A 2 1B + 0A 3 1B + 1A 4
- Gel indicated that no ligation occured. There are several reasons for this:
- 1. We only have a 2 bp overhang
- 2.We are not using enough enzyme
- 3. We did not use enough buffer
- Gel indicated that no ligation occured. There are several reasons for this:
- Enough buffer was added and enzyme was doubled, left to react over the weekend
