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Team:University of Ottawa/6 August 2008
From 2008.igem.org
Today in the Lab
Chris
Minipreparation and Confirmation of PTP2/PSSA42 samples
- Control (tip only in LB+Amp) was clear
- Followed miniprep protocol included in kit
- Spilled one tube; concentration expected to be zero. Also, did not mix neutralisation solution as outlined in the protocol.
- Measure absorbance of isolated DNA: all samples very low and poor purity.
- Digested aliquots of all samples with PstI despite low concentrations. Expected bands (1246, 699, 5685) appeared on a 1% gel; however were faint and inconclusive.
- While the gel was running, performed another miniprep of the same ten samples, following protocol exactly in order to increase DNA concentrations.
Continuation of Ligation Optimisation
- Ran all products on a 1% gel for 40 minutes at 80V.
- All ligations appeared exactly the same on the gel; future ligation protocol will be shortened from overnight to a minimum of six minutes.
