Team:Warsaw/Calendar-Main/12 August 2008

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Checking for presence of protein A on the cell membrane

Emilia

Inoculation of pACYC177+OmpA_A_alpha, pACYC177+OmpA_Z_alpha and pACYC177+OmpA_omega_ΔA_alpha (with and without induction).

Purification of proteins: Z-alpha and Z-omega

Piotr

  1. Bacteria centrifuged and sonicated (according to protocol).
  2. Sonicate centrifuged.
  3. Supernatant discarded, pellet resuspended in ddH20, centrifuged and again resuspended in ddH20.