Purification of proteins: A-alpha

Piotr

Liquid LB with chloramphenicol and ampicillin was inoculated with Rosetta strain carrying pET15b+A_alpha.

Blue-white screening and rifampicin test in GM2163

Michał L., Ewa

Blue colonies of Top10 strains transformed with pZC320 from various GM2163 strains were counted.
Results (% of blue colonies):
pMPMT5-omega 100%
pMPMT5-AID 100%
pMPMT5-AIDT7 50%
pMPMT5-AID+T7 0% (weird)