Preparation of constructs: OmpA_alpha and OmpA_omega
Michał K.
- Isolation of plasmids from cultures inoculated on previous day.
- Control digest of isolated plasmids with PstI (Orange buffer).
- Gel electrophoresis (Fig. 1 and 2), no proper colonies found.
Fig. 1. Lanes 1 to 8 - control digest of 8 clones carrying hypothetical OmpA_alpha fusion with PstI.
Fig. 2. Lanes 1 to 8 - control digest of 8 clones carrying hypothetical OmpA_omega fusion with PstI.
Preparation of alpha-A and omega-A fusions
Michał L., Ewa, Marcin
Universal PCR program for protein A, alpha and omega
| Temperature | Time |
|---|
| 94°C | 4:00 |
| 94°C | 0:30 | 28 cycles |
| 50°C | 0:45 |
| 72°C | 2:00 |
| 72°C | 10:00 |
| 4°C | infinite |
|
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