Team:Warsaw/Calendar-Main/6 June 2008

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Blue/white and rifampicin test #1

Michał L., Ewa, Marcin

Making chemocompetent E. coli TOP10 carrying pZC320.


Preparation of pMPMT5-AID+AIDT7 construct

Michał K.

  1. Isolation of hypothetical pMPM-T5-AID+AIDT7.
  2. Control Digest of pMPM-T5-AID+AIDT7 with BamHI (BamHI buffer).
  3. DNA gel electrophoresis. Fig. 1

Fig. 1. Control digest of the pMPMT5-AID+AIDT7 construct.
Lane 2 - 5: isolated plasmids digested with BamHI