Preparation of pMPMT5+AID construct
Michał K.
- Restriction digest of pTrc99A-AID with HindIII and NcoI (1x Tango buffer).
- DNA electrophoresis and isolation of DNA encoding AID from proper band (600 bp). Fig. 1.
- Restriction digest of pMPM-T5 with HindIII and NcoI (1x Tango buffer), dephosphorylation (CIAP).
- DNA electrophoresis and isolation of pMPM-T5 vector from proper band (4250 bp). Fig. 2.
- Electrophoresis to estimate the concentration of isolated DNA.
- Ligation of isolated DNA fragments.
Fig. 1. 1-DNA ladder; 2-pTRC99a-AID digested with NcoI and HindIII
Fig. 2. 1-DNA ladder; 2-pMPM-T5 digested with NcoI and HindIII
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