Utah State/15 July 2008

From 2008.igem.org

None of the eight transformations from yesterday worked. An ampicillin plate from the batch we made yesterday was streaked with previously successfully transformed with T7 luciferase E. coli to see if the plate is the cause of failure. A new box of Top 10 competent cells were used yesterday, and that is also a possible source of problems. Today, the same transformations are being attempted again with the addition of the E0240 plasmid from the newer iGEM paper (should be the same as that from plate 1001-4B) that was mailed, a full GPF construct from that same paper - I20260, and the RFP - I13521 (previously successfully transformed) as a control.