Virginia/8 July 2008
From 2008.igem.org
Goals
- Prepare overnight broth of single successful colony of Promoter + RBS transformation
- Plate Screening plasmid (psb1a10) when it arrives from iGem
- Try again with ligation of RFP and GFP enzyme cut DNA
- Transform this ligation and pray that it grows in the incubator
- Run a gel of linearized and cut DNA of BP1 and terminator assembly to verify transformation
- Begin ligation of standard RBS with BP1 gene to begin parallel assembly of our final operon
Notes
- Maxiprepped B0015 and B0034 smell strongly of alcohol, we are dubious of the content of the prepared DNA
Accomplished
- Starting from maixprepped DNA we cut:
- E0240, I715039, B0034 (dubious, see above), BP1
- Short Ligations: (45min)
- Test Vector 1: I715039 + E0240
- Did a ligation each for non-purified DNA (after digestion) and purified DNA.
- B0034 (RBS) + BP1
- Only non-purified post-digestion DNA
- Plated all short ligations
- Long Ligations: (overnight @ room temp)
- Test Vector 1: I715039 + E0240 (unpurified)
- B0034 (RBS) + BP1
- Plated screening plasmid pSB1A10 that we received today
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