Brown: Team Resistance/26 October 2008

Biobricking work

 * Made new culture of the PVJ4 transformation so that a miniprep can be done.
 * New culture of gene S105 part made.

Began transforming promoters from the registry in order to ligate them to our cassette and create composite parts:
 * pBAD prmoter
 * IPTG inducible promoter
 * Mercury inducible promoter
 * Pu inducible promoter (xylene induced)
 * T7 promoter, with activity modified by change in temperature

Also re-transformed ligations of the pVJ4 cassette and the S105 cassette (both transformations unsuccessful in the past)


 * Will run a gel of the S105 gene restriction digest with EcoRI and SpeI for verification of part. The gel showed a faint band at the length we hoped for but there was no other band verifying the vector. We should have seen two bands but we only saw one.

Conductivity Tests

 * PVJ4 Colony 2 and 4 plus Controls. 10ml cultures.  420 ul of 50% Arabinose solution will be added.  Conductance measurements will be taken before and after lysis has occurred.
 * Throughout the test, Optical Density measurements will be taken.