Team:Warsaw/Calendar-Main/20 June 2008

Preparation of constructs: OmpA_alpha and OmpA_omega Michał K.  Isolation of plasmids from cultures inoculated on previous day. Control digest of isolated plasmids with PstI (Orange buffer). Gel electrophoresis (Fig. 1 and 2), no proper colonies found.

 Fig. 1. Lanes 1 to 8 - control digest of 8 clones carrying hypothetical OmpA_alpha fusion with PstI.

<img src="http://2008.igem.org/wiki/images/1/1a/Omp_control_digest2_WAW.jpg" width=400/> Fig. 2. Lanes 1 to 8 - control digest of 8 clones carrying hypothetical OmpA_omega fusion with PstI.

Preparation of alpha-A and omega-A fusions Michał L., Ewa, Marcin