Purdue/16 October 2008

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Performed another ligation (for protocol, see Sept. 25). It turns out that the competent cells we used before are lacZ+. :(

Therefore, both UV-exposed and non-exposed bacteria were turning blue because they were on X-gal plates.

We were able to try a different method of transformation: Dr. Clase provided us with some Bio-Rad Hb101 cells (which naturally take up plasmids). We are following the Bio-Rad protocol for transformation:
 * 1) Put 250uL transformation solution (CaCl2) into an eppendorf tube
 * 2) Put tube on ice
 * 3) Add colony from starter plate of Hb101 cells to tube
 * 4) Incubate on ice 10 min.
 * 5) Heat shock at 42C for 50 seconds
 * 6) QUICKLY move back to ice and incubate for 2 minutes
 * 7) Remove from ice, add 250uL LB broth, incubate at RT for 10 min.
 * 8) Tap to mix, plate 100uL on amp plate

Edited by Janie Stine