Team:Hawaii/Notebook/2008-06-13

= Things we did today =

Prepped Cells for Glycerol Stock/Seed Stock

 * Krystle
 * Inoculated a single colony in each 10 mL LB + antibiotic

Prepped Cells for Competency

 * Grace


 * Checked yesterday's inoculation. OD600=1.2 (too high). Need to redo.
 * Made SOB for new inoculation.
 * Inoculated 250 &mu;l SOB with 100 &mu;l DH5&alpha;. Inoculated 250 &mu;l SOB with 10 &mu;l DH5&alpha;. Incubated overnight at 27C with shaking (150 rpm).

Cell density test

 * Grace


 * Retrieved plates from SC incubator. All plates had too many colonies (lawn). Need to redo.
 * Made BG-11 plates for retest next week.

Made LB agar stock

 * Grace


 * (2) 300 mL batches of LB + 1.5% Agar. Stored in 4C.

= Discussion =
 * We need to make more BG-ll 50x stock solution and TES 50x stock solution
 * Kelly from SC lab will be in 12pm-3pm. We should retrieve our triparental conjugation plates then.
 * Grace, Krystle, and Norman will come in at 9am tomorrow to plate triparental conjugates, make competent cells, and glycerol stock purified Biobrick colonies.

= Quote of the Day =