Cutting a Specific Band from Agarose Gel



A gel in the UV light box prior to cutting




 * After running the restricted plasmid on agarose gel (prefereably with a wide comb to increase well size), identify the specific band to be cut out.


 * Weigh a DNase-free 1.5 ml microcentrifuge tube and record the weight.


 * Place the gel in a UV light box (N.B. wear gloves and a UV filter mask and ensure sleeves are completely covering wrists to avoid direct UV light exposure).


 * Using a sterilised scalpel blade, long wavelength(365 nm) and minimal exposure time, cut out the required band, including as little of the surrounding gel as possible.


 * Place agarose gel band into a DNase-free 1.5 ml microcentrifuge tube.
 * Weigh the microcentrifuge tube plus agarose band and calculate the weight of the actual agarose slice.


 * Proceed to Purifying DNA from Gel Slices.