Team:UNIPV-Pavia/Protocols/Lb

The protocols we used


 * LB medium preparation
 * Plasmid resuspension from IGEM paper spots
 * Transformation
 * Plasmid extraction
 * BioBrick digestion with restriction enzymes
 * DNA gel extraction
 * DNA precipitation with sodium acetate
 * Antarctic Phosphatase
 * Ligation
 * PCR

LB medium preparation (Estimated time: 10 min + 4 hours of autoclavation and cooling) Materials needed:
 * NaCl
 * BactoTryptone
 * Yeast extract 
 * ddH2O
 * Agar
 * Clean bottle or E-flask
 * For a final volume of 1 l, put:
 * 10 g NaCl
 * 10 g BactoTryptone
 * 5 g Yeast extract
 * 15 g Agar (only for LB plates)
 * into 1 l of ddH2O.
 * Autoclave the solution.
 * Let it cool to ~40-45°C
 * Add the proper antibiotic if needed.
 * ONLY FOR PLATES:
 * Pour an homogenous layer of agar LB into Petri plates under the hood.
 * Let agar LB polymerase.
 * Cover and store at +4°C.
 * Always check for contaminations before using!