Team:Chiba/jk/γ/week 4

実験ログ 出力班

7,September,2008
Digestion
 * Cloning
 * vector:BBa_R0040(ptet)① 75ng/μl
 * insert:BBa_S03158(RBS+RhlR)② 33.6ng/μl
 * insert:BBa_S03156(RBS+lasR)③ 33.6ng/μl
 * insert:Ba_S03160(RBS+cinR)④ 38.4ng/μl
 * vector:BBa_R0010(pLac)⑤ 100ng/μl
 * insert:BBa_J52008⑥ 33.6ng/μl

-->37℃ 3hour

-->Gel Extraction

-->DNA purification (①-④,⑥:7μl ⑤:6μl)

8,Septmeber,2008
SAP
 * BBa_R0040(ptet)①
 * BBa_R0010(pLac)⑤

-->37℃ 1hour

-->65℃ 30min

-->DNA purification

-->21μl 6μl

-->Gel check


 * vector:BBa_R0040(ptet)① 75ng/μl
 * insert:BBa_S03158(RBS+RhlR)② 33.6ng/μl
 * insert:BBa_S03156(RBS+lasR)③ 33.6ng/μl
 * insert:Ba_S03160(RBS+cinR)④ 38.4ng/μl
 * vector:BBa_R0010(pLac)⑤ 100ng/μl
 * insert:BBa_J52008(RBS+rLuc)⑥ 33.6ng/μl

10,September,2008
TIME Responce(liquid)

TIME Responce(solid)

Colony PCR

-->Gel check

-->Mini prep

13,September,2008
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