Team:Mississippi State/4 August 2008


 * 1) Repeat digestion protocol using NotI, EcoRI, LiP, and pPIC6
 * 2) prepare and run 1% agarose gel, cut out products
 * 3) perform gel purification using QIAgen kit
 * 4) prepare and run 1% agarose gel
 * 5) grow x-33 yeast in YPD medium overnight
 * 6) make ligation mix with T4, Lip, and pPIC6
 * 7) grow overnight culture of xl1blue
 * 8) do transformation using ligation mix, spread onto plates, incubate overnight