TUDelft/21 August 2008

=August 21st=

Pfx PCR on Eschirichia coli genes
I tried a PCR on the atoB, idi and ispA genes using another enzyme, Pfx. This enzyme has proof reading whereas Taq does not have it. The PCR program used is the same as on August 20th except for the elongation step, where 68ºC in stead of 72ºC was used. Also there is no mastermix available with Pfx enzyme, so MgCl, Pfx, buffer and dNTP's have to be added seperately. This was done according manufacturer's protocol.

The PCR products did not show any band on gel, so it will be tried again.