Team:Hawaii/Notebook/2008-08-21

= Things we did today =

Construction of p+r, r+g, g+t

 * Grace


 * Annealed oligonucleotides:
 * plac (I14032) for front ligation
 * rbs 1.0 (B0034) for back ligation
 * rbs 0.6 (B0030) for front ligation
 * tt (B1006) for back ligation
 * Ligated:
 * nir (SP cut) + B0034
 * plac + B0034 (EX cut)
 * B0030 + E0040 (EX cut)
 * E0040 (SP cut) + B1006
 * Placed in 37C water bath for 1 min, vortexed lightly, spun down before adding ligase
 * Transformed DB3.1 cells using 5 &mu;l ligation reaction

BB-pRL1383a MCS re-replacement

 * Grace


 * Inoculated LB+amp100 with J33207 for plasmid prep tomorrow

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"