Team:Hawaii/Notebook/2008-08-20

= Things we did today =

Name of Task

 * Margaret & Krystle


 * Annealed and phosphorylated:
 * plac (I14032) for front ligation
 * rbs 1.0 (B0034) for back ligation
 * rbs 0.6 (B0030) for back ligation
 * rbs 0.6 (B0030) for front ligation
 * tt (B1006) for back ligation


 * We decided to perform this reaction again with out gel purification and the gel was discarded.
 * These annealed products can be found in the -20&deg;C freezer in the DNA box.
 * Quantification was achieved simply by adding up the amount of DNA added.
 * plac (I14032) for front ligation --> 370ng/ul
 * rbs 1.0 (B0034) for back ligation --> 206.7ng/ul
 * rbs 0.6 (B0030) for front ligation -->
 * rbs 0.6 (B0030) for back ligation --> 228.3ng/ul
 * tt (B1006) for back ligation --> 375ng/ul

Restriction Digest

 * Krystle
 * B0034 with EcoRI and XbaI (for ligation with plac)
 * nir with SpeI and PstI (for ligation with RBS)
 * gfp wih SpeI and PstI (for ligation with TT)
 * gfp with EcoRI and XbaI (for ligation with RBS)

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"