Team:University of Lethbridge/Notebook/Project3September

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September 30, 2008
Andrew

PCR of genomic + rpsA DNA with two different sets of primers

Reaction Conditions: - 1.0 uL Template DNA - 5.0 uL 10x Buffer - 2.0 uL 10 mM dNTPs - 1.0 uL Forward Primer - 1.0 uL Reverse Primer - 0.5 uL Econo taq polymerase - 39.5 uL Optima H2O

PCR carried out for 1/10 and 1/100 dilutions of genomic DNA and rpsA DNA

Cycling Conditions: 1. 94 C 2 min 2. a. 94 C 15 sec b. 47 C 15 sec c. 72 C 15 sec 3. Repeat step 2 for 25 cycles 4.72 C 5 min