Team:Cambridge/Signalling/Vectors

   

All these vectors were kindly provided by Dr. Dan Ziegler, Director of the Bacillus Genetic Stock Center at Ohio State University.

= Integration Vectors =

These vectors integrate into the chromosome and do not have a replication origin in Bacillus. They either integrate cassettes that require double crossovers and two homologous regions, or whole-plasmid insertions that only require one crossover and one corresponding region of homology.

Ectopic Integration
The integration site of these is pre-determined

Insert Integration
The user inserts a homologous piece of the Bacillus chromosome and the vector integrates there.

= Shuttle Vectors =

Plasmid Gels]
   
 * We ran these plasmids on gels, using double and single digests, to check them