Team:BCCS-Bristol/Protocols-DMSO stocks



DMSO stocks

 * 1) 	Make an overnight culture of the bacteria in LB broth (If the strain carries a resistance gene on a plasmid, you have to use LB with antibiotics)
 * 2) 	Next morning, put 70 µl DMSO into a cryotube that you labelled with the strain’s name and the date
 * 3) 	Add 930 µl overnight culture
 * 4) 	Incubate immediately for 30 min on ice
 * 5) 	Store the DMSO culture in the -80°C freezer
 * 6) 	In the evening (or in one of the following days), make a streak out of the stock to see whether the bacteria are ok