Team:Paris/September 9

=Checking our ligases (following)=

Ligation (2 hours at 16°C)

 * 4 µL DNA
 * 2 µL 10X buffer 2
 * 1 µL T4 DNA ligase
 * 13 µL water

Transformation

 * Strain used: Chemically competent E. coli DH5 alpha cells
 * 100 µL of cells
 * 5 µL of ligation products
 * 30 min on ice
 * heat shock: 45 s at 37°C
 * 2 min on ice
 * 900 µL of SOC
 * 1 hour at 37°C
 * plating (1 µL or 100 µL) on LB plates containing ampicilline