Team:Edinburgh/Project/iGEM 2008 Labwork Summary/Our Primers

Our Primers
This is the current situation with regard to primers:

Primer sequences:
Cellulomonas fimi endogluconase A (cenA) Suitable BioBrick primers should be:

Forward:   CGT gaattc gcggccgc t tctagATG TCC ACC CGC AGA ACC Reverse:   CGT t actagt a TTA TTA CCA CCT GGC GTT GC

Cellulomonas fimi endogluconase B (cenB) Suitable BioBrick primers should be:

Forward:   CGT gaattc gcggccgc t tctagATG CTC CGC CAA GTC CC Reverse:    CGT t actagt a TTA TTA GTT CGT CGG GAT G

Cellulomonas fimi endogluconase C (cenC) Suitable BioBrick primers should be:

Forward:   CGT t tctagATG GTT TCT CGC AGG TC  Reverse:    CGT ctgcag cggccgc t actagt a TTA TTA GCT GCG CGG ACG C

Cellulomonas fimi exogluconase (cex) Suitable BioBrick primers should be:

Forward:   CGT t tctagATG CCT AGG ACC ACG CC  Reverse:    CGT ctgcag cggccgc t actagt a TTA TTA GCC GAC CGT GCA GG

E. coli glucose-1-phosphate adenylyltransferase (glgC) Suitable mutant primers are:

to remove the first forbidden EcoRI site:

Forward:   tgttgaaaaacctgctaacc - 54°C Reverse:   aattcgataattttatcgttc -52°C

 to remove the second forbidden EcoRI site:

Forward:   ctcattctgcaacattgattcc - 54°C Reverse:   ttcacgcgaacgcgcg - 54°C

to cause 336:Gly->Asp:

Forward:   acggttgtgtgatctccg - 54°C Reverse:   cggaaaccagtgagttaag- 56°C

Arabidopsis thaliana isoamylase 1 (isa1) Suitable BioBrick primers should be:

Forward:   CGT t tctagATG GAT GCA ATC AAA TGC AG - 56ºC Reverse:   CGT ctgcag cggccgc t actagt aTTA TTA GGG GTC TTT AAT TGG TG - 58ºC

Arabidopsis thaliana isoamylase 2 (isa2) Suitable BioBrick primers should be:

Forward:   CGT ttctagATG GCT GCG TGG TCA CC - 56ºC Reverse:   CGT ctgcag cggccgc t actagt a TTA TTA AGC GGT AGT ATT GAT GG - 54ºC

Arabidopsis thaliana granule bound starch synthase 1 (GBS1) Suitable BioBrick primers should be:

Forward:   CGT gaattc gcggccgc t tctagATG GCA ACT GTG ACT GCT - 54ºC Reverse:   CGT t actagt a TTA TTA CGG CGT CGC TAC G - 52ºC