Team:Johns Hopkins/Notebook/GROUP 6: Vectors

GROUP 6: Vectors
Status report by Tejas on 8/5/08 Vector transformed into bacteria strain DB3.1: Yes. Pre-transformed by MIT and delivered in STABS Permanent culture made in the Boeke lab for future reference: No Selectable marker for this vector: (Amp, Kan, Cam) Medium made and tested: No (link) DNA preps made: Yes DNA preps tested by RE digest: Yes, Vectors (CAM,AMP,KAN3,KAN4) after Digest DNA preps tested by transformation into DB3.1 and DH5alpha (or JM109): No (currently being done) Sample format/data follows: Amount transformed		cfu/micGm in DB3.1		cfu/micGm in JM109 0.1 ng					5 * 10e7			<2 * 10e2 Preparative digests ready for use are located – where?