Team:ESBS-Strasbourg/4 August 2008

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WetLab
Katja Construction of the operator BioBricks (GAL4O, LexAO, LacO, CIO, TetO)
 * hybridisation of the ordered oligos
 * ligation into the XbaI, PstI cutted vector pSB1A2 (using protocol 2c)
 * transformation into competent TOP10 and plating on LB-Amp (using the heat-shock protocol)

Robin - PCRs of Cin8, Tup1, Cln2, Hsl1, Cyc100-16