Team:ESBS-Strasbourg/Ligation

1)Remarks:

2)Protocol:

see iGEM protocol
 * For insert/vector ligation:

5 T4 DNA ligase unit, for 25-50ng of DNA
 * For intra vector ligation:

-> vortex and spin down -> 1h at 22°C water bath (in 4°C room) -> enzyme inactivation at 65°C for 10 min -> directly usable for the transformation
 * 2c) Protocol according to Fermantas
 * 50-400 ng vector
 * 1 to 3 times the molar amount of the insert
 * 2 µl ligation buffer
 * 1 µl ligase
 * to 20 µl water

Protocols