Team:Hawaii/Notebook/2008-10-16

= Things we did today =

Verification of transformants

 * Grace


 * Colony PCR of transformants
 * None of the colonies tested positive for their desired inserts

Transformation

 * Margaret

Construction of GFP secretion devices

 * Krystle
 * Gel purified restricted nir+ rbs and slr+gfpf
 * Dephosphorylated cut pSB1A3 vector
 * Ligation
 * nir+rbs with slr+gfpf
 * lac+rbs with slr+gfpf
 * Transformation into home-made competent and commercially competent DH5α cells and plated on LB amp100 plates

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"