Team:Hawaii/Notebook/2008-06-10

= Things we did today =

Made LB+Kan50 plates

 * Grace


 * Made LB+Kan50 plates

Preparation for tri-parental conjugation

 * Grace
 * Streaked out DH5&alpha; transformed with pRL1383a on LB+sp for conjugation tomorrow
 * Streaked out E. coli transformed with RP1 on LB+kan for conjugation tomorrow

Biobrick extraction/transformation

 * Grace
 * Redid yesterday's BioBricks transformation using BBa_B0034, encoding a RBS. Gold standard super competent cells and pUC18 were obtained from GP for comparison of transformation efficiency with our competent cells. (see experiment)
 * Norman


 * Streaked out transformants from yesterday's BioBricks transformation/extraction

Drylab Work

 * Took cyanobacteria plates from PCC6803 transformation and PCC6803 cell density test to SC's lab for CO2 incubation
 * Got Anabena triparental conjugation protocol from SC
 * Found nifty paper on cyanobacteria proteins/gene location.

Discussion

 * Norman will figure out concentration of DNA on Biobricks filter paper
 * Norman will make LB+amp100 plates tonight
 * Need to check incubated plates tomorrow (Krystle?)
 * Need to create growth curve (OD750 vs. cell population)
 * Need to practice lifting agar for antibiotic insertion (Thursday?)

Quote of the Day
"As long as you don’t know what it is, you don’t need to fill out forms."