Team:UNIPV-Pavia/Protocols/GelExtraction

The protocols we used


 * LB medium preparation
 * Plasmid resuspension from IGEM paper spots
 * Transformation
 * Plasmid extraction
 * BioBrick digestion with restriction enzymes
 * DNA gel extraction
 * DNA precipitation with sodium acetate
 * Antarctic Phosphatase
 * Ligation
 * PCR

DNA gel extraction (Estimated time: 1 hour and 30 min) Materials needed:
 * Agarose gel with well-separated, sharp bands
 * Scalpel and tweezers to cut gel (they have to be cleaned in ddH2O after every cut)
 * Roche Agarose Gel DNA Extraction Kit
 * Follow Roche standard protocol: Roche Agarose Gel DNA Extraction Kit protocol