Alberta NINT/28 May 2008

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lab work (SD):

Transformed XL1-B cells with the plasmids created 27/05/08. K102002 had 170 colonies. K102004 had 629 colonies. K102000 negative control had 5 colonies.

lab work (JD):

Carried out mini-preps on O/Ns Digested R0011 with SpeI+ PstI Ran gel electrophoresis and cut out desired band.

lab work (WM): Ligated I0500/(SpeI + PstI) with TA0In/(XbaI + PstI) Transformed 2 ul into 100 ul XL1-B. Plated 150 ul on LB + kan35