Team:Chiba/Calendar-Home/5 September 2008

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4 September 2008 <|> 6 September 2008

Team:Input
no work

Team:Communication

 * (4/9)--->Liquid Culture
 * Cultured the following cells (2mL LB-Amp, at 37&deg;C)
 * from transformed plates:
 * BBa_K084009(Plac+RBS+RhlI+LVA, Competent Cells : JW1908)(r1, r3~r7)
 * BBa_K084010(Plac+RBS+RhlI, Competent Cells : JW1908)(c1,c2,c4~c8)
 * from Glycerol Stock:
 * BBa_S03623(Ptet+RBS+LuxI, Competent Cells : JW1908)


 * --->Phenotype test
 * MIX


 * Incubated for 8hours at 37 degrees
 * The measurement of the intensity GFP by Fluoroskan Ascent 2.5(program:Ascent Software Version 2.6)


 * Spindown (max rpm, 3 min)
 * The measurement of the intensity GFP(visual judgment) (UV 312nm)

Team:Output
TIME RESPONCE(liquid)

Digestion test
 * BBa_J52008+BBa_F2620 x2
 * BBa_E2030+BBa_F2620 x2

-->37&deg;C 30in

Gel Check
 * BBa_J52008+BBa_F2620 x2(1)
 * BBa_E2030+BBa_F2620 x2(2)
 * BBa_J52008(PCR Product)(3)
 * BBa_E2050(4)


 * Luciferase(BBa_J52008+BBa_F2620) check-->no light