Newcastle University Wetlab/15 August 2008

Friday 15th August
pGFPrrnB, pJWV021 and BBa_I746107 were restricted using 20μl plasmid and 1μl each enzyme in 40μl total reaction volume: - pGFPrrnB restricted with EcoRI + NheI

- pJWV021 restricted with NheI + BglII

- pGFPrrnB restricted with EcoRI + SpeI

- BBa_I746107 restricted with EcoRI + SpeI

These were incubated at 37˚C for 1 hour and run on a gel. The gel showed that pGFPrrnB and pJWV021 had restricted well with EcoRI + NheI and Nhe +BglII respectively, but pGFPrrnB and BBa_I746107 restricted with EcoRI + SpeI had only partially restricted.