Team:Hawaii/Notebook/2008-10-10

= Things we did today =

Verification of Transformants

 * Grace


 * Colony PCR of transformants
 * nrgt #15

Overnight RE digest

 * Grace


 * EcoRI/PstI:
 * BBpRL1383a-1
 * nrsg #6 (PCR)
 * prpgt #5, 7, 11 (PCR)

Triparental Conjugation

 * Grace


 * Began triparental conjugation between E. coli containing RP1 and BBpRL1383a -1 or -18 and Synechocystis
 * RP1 OD600=0.6341
 * BBpRL1383-a OD600=61.25
 * BBpRL1383a-18 OD600=0.4884
 * Synechocystis OD700=0.8629, OD730=0.7337
 * Placed plates in SC 37C CO2 incubator until Monday

Verification of plasmids

 * MARGARET




 * Why haven't I gotten good efficiency of transformation? I ran a gel of the plasmids i am working with and got the answer. Apparently there was a mix-up and I was using low quality plasmid.
 * The pSB1A3 and pSB3K3 digested and de-phosphorylated (lanes: ) were thrown out. Norman's pSB1A3 and pSB1A7 were digested with E and P today at 4:30pm.
 * The base vector is digested--> bands appear to be correct size.

Submitted sequencing

 * Margaret


 * the plac/rbs/rep colony 7 construct and oriV colony 1 (from most recent transformations) were sent in for sequencing today.

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"