Team:Warsaw/Calendar-Main/22 October 2008

Preparation of AID under pBAD/araC (BBa_K103002) Michał K.  Isolation of plasmids from cultures inoculated on previous day - pSB1A3 + AID under pBAD/araC (BBa_K103002).

Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - no proper clones found. We didn't manage to obtain this BioBrick.

Preparation of <a href=http://partsregistry.org/Part:BBa_K103014>OmpA_linker_alpha_linker (BBa_K103014)</a> Michał K.  <a href=http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inoculated on previous day - <a href=http://partsregistry.org/Part:pSB2K3>pSB2K3</a> + <a href=<a href=http://partsregistry.org/Part:BBa_K103014>OmpA_linker_alpha_linker (BBa_K103014)</a>.</li>

Control <a href=http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found. <a href="http://2008.igem.org/Team:Warsaw/Calendar-Main/22_October_2008#fig1">Fig. 1</a>.</li></ol>

<img src="http://2008.igem.org/wiki/images/4/44/Traw_22_10_2008.jpg"></a> Fig.1.Control EcoRI/PstI digests of isolated plasmids. 1. Marker 2-9. EcoRI/PstI digests of isolated plasmids

Preparation of <a href=http://partsregistry.org/Part:BBa_K103016>OmpA-linker-omega-linker (BBa_K103016)</a> Piotr

 <a href=http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inoculated on previous day <a href=http://partsregistry.org/Part:BBa_I739204>pACYC177</a> + <a href=http://partsregistry.org/Part:BBa_K103016>OmpA-linker-omega-linker (BBa_K103016)</a>.</li>

Control <a href=http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.</li> </ol>