User:University of Washington/5 September 2008

LuxR from AraC and TetR(Faifan)
- measured Absorbance of the overnight culture at 660 nm = 1.5

- diluted 60 ul culture into 5.40 ml Tsy+KAN. Absorbance at 660 nm = 0.035

- incubated 37 degree Celsius for 2.5 hrs, Absorbance at 600 nm = 0.7

- diluted 1 ml culture into 5 ml Tsy+Kan, OD@600nm = 0.165

- set up 96 well plate reader experiment:
 * 5x5, 160 ul culture


 * added dH2O to final the volume to 200 ul
 * added 40 ul of mineral oil to each well to protect evaporation
 * set the machine to run 24 hrs, read every 10 mins

Conjugation (Scott)
Conjugation method #2 ·pAC99 + pUB307

Glycerol Stock ·pAC99 ·pAC99 + pUB307

Back to Team:University_of_Washington/Notebook