Team:University of Ottawa/27 June 2008

  Untitled Document  

 Home  Welcome Announcements</li> </ul> The Team</a> <ul> Who We Are</a> <ul> Advisors</a></li> Undergrads</a></li> </ul> </li> What We've Done</a></li> Where We're From</a></li> Contact Us</a></li> </ul> </li> The Project</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Project_Overview">Overview</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Pulsate_Gene_Expression">Expression</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Cell-to-Cell_Communication">Communication</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Oscillatory_Dynamics">Oscillation</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Applications">Application</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Journal_Club">Journal Club</a></li>

</ul> </li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Parts">BioBricks</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Modeling">Modeling</a></li> <li><a class="MenuBarItemSubmenu" href="http://2008.igem.org/Team:University_of_Ottawa/Wet_Lab">Wet Lab</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Lab_Protocols">Lab Protocols</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/WetWare">WetWare</a></li> </ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Notebook">Notebook</a></li> <li><a class="MenuBarItemSubmenu" href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors">Sponsors</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Acedemic_Sponsors">Academic</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Research_Sponsors">Research</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Corporate_Sponsors">Corporate</a></li> </ul> </li>

</ul> <script type="text/javascript">

Today in the lab
Dan
 * Ligation
 * <li> Ligation was performed on the digested 0 1 A B PCR products, new samples were created according to the following legend:
 * {| class="wikitable"


 * Components || New Name
 * 0B + 0A || 1
 * 0B + 1A || 2
 * 1B + 0A || 3
 * 1B + 1A || 4
 * }
 * <li> Gel indicated that no ligation occured. There are several reasons for this:
 * 1. We only have a 2 bp overhang
 * 2.We are not using enough enzyme
 * 3. We did not use enough buffer
 * <li>Enough buffer was added and enzyme was doubled, left to react over the weekend
 * <li> Gel indicated that no ligation occured. There are several reasons for this:
 * 1. We only have a 2 bp overhang
 * 2.We are not using enough enzyme
 * 3. We did not use enough buffer
 * <li>Enough buffer was added and enzyme was doubled, left to react over the weekend