Team:Hawaii/Notebook/2008-09-15

= Things we did today =

PCR from 9/13

 * Margaret


 * Rep, oriV- correct size, so restriction digest with X,P of PCR product
 * omega- correct size, but still has a ladder, so re-digest of plasmid prep with S, X
 * oriT did not show up, but I found a re-digest from 7/24 that I will use for further cloning
 * positive control did not work, not sure if negative control worked or if I did something wrong.

Plasmid prep

 * Grace


 * Inoculated TB+amp100 with:
 * 3A: nir+rbs+slr1 #2, 3
 * 3A: nir+rbs+pilA #6
 * 3A: nir+rbs+slr1+GFPf #10
 * 3A: nir+rbs+pilA+GFPf12 # 2, 6
 * 3A: nir+rbs+pilA+GFPf13 # 4, 6, 8, 10
 * Inoculated TB+sp100
 * BB-pRL1383a

Sequencing

 * Grace


 * ExoSAP'd, determined DNA conc., prep'd for sequencing:
 * 3A: nir+rbs+slr1 #2, 3
 * 3A: nir+rbs+pilA #6
 * 3A: nir+rbs+slr1+GFPf #10
 * 3A: nir+rbs+pilA+GFPf12 # 2, 6
 * 3A: nir+rbs+pilA+GFPf13 # 4, 6, 8, 10
 * plac+rbs+slr1+GFPf #8
 * Sent in samples from today and from last week for sequencing by CORE Hawaii

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"