Team:Hawaii/Notebook/2008-08-19

= Things we did today =

RE test

 * Krystle


 * Checked to see if RE are still active by cutting R0010 with EcoRI, XbaI, SpeI, and PstI individually
 * Ran on 2% agarose gel
 * R0010 was probably from a plasmid prep where cells did not lyse, i.e. no DNA in prep
 * Oops, forgot to run uncut plasmid as control
 * Set up new RE digest (w/ C0012) overnight to check enzymatic activity
 * Also checked EcoRI enzyme in TKW box of lab freezer

Verification of transformants

 * Grace


 * Plasmid prep of BB-pRL1383a+J33207 plasmid to verify replacement of GFP with J33207 (lac device)
 * PCR of BB-pRL1383a+J33207 plasmid preps
 * Negative = water
 * Positive = BB-pRL1383a
 * Ran on 2% agarose gel
 * No replacement of GFP; enzymes didn't cut?

Updated project descriptions on wiki

 * Krystle

= Discussion =

= Quote of the Day = "What?!? Grrr...... - Krystle"