TUDelft/13 October 2008

=October 13th=

Growing 25, 30 and 37ºC cultures
For our luciferase assay to include temperature of 25ºC, 30ºC and 37ºC, we'll grow all our cultures (K115012 & K115029-36) in fourfold over the weekend at 20ºC in LB medium. All of them are induced by arabinose (although not strictly necessary as shown here) and are grown on ampicillin.

Harvesting 20ºC culture
We've harvested and lysed the cells as described in the protocol. Sonication amplitudes varied from 8 microns to 10 microns.