Team:Hawaii/Notebook/2008-09-20

= Things we did today =

Construction of secretion device (cont.)

 * Grace


 * Ran RE digests on 4% agarose gel
 * Extracted bands from gel
 * Ligated overnight:
 * nir+rbs & pilA & pSB1A3
 * nir+rbs & slr1+GFPf & pSB1A3
 * slr1+GFPf & tt & pSB1A3
 * rbs & slr1+GFPf & pSB1A3
 * plac & rbs+GFP & pSB1A3
 * rbs+GFP & tt & pSB1A3
 * pSB1A3 to self (control)

Sequencing

 * Grace & Krystle


 * Figured out Krystle's sequencing results
 * GFPf+tt 17F+8R = ccdB base vector
 * GFPf+tt 8F+21R = rbs (B0030+GFPf)
 * GFPF+tt 21F+17R = GFPf+B1006
 * Need to resequence #21, 17 because no way to tell which is correct (F/R)

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"