User:University of Washington/1 July 2008

LuxR from AraC and TetR
- AraC sequences matched the biobrick (BBa_R0080).

LuxR from pLAc
- Miniprep done on R0010 and J04430 transformed e.coli. DNA was submitted for sequencing of the parts.

- Part I0462 was again transformed into a different e.coli strain.

BioBrick Promoter Measurements
- All overnight cultures showed successful transformation by growing on plates with kanamycin.

- Single colonies were added to 5 mL of TSY media, then incubated at 37 degrees Celsius in a rotator for 5 hours.

- 750 uL of the incubated cells were added to 750 uL of 40% glycerol, incubated at room temperature for 30 minutes, then stored at -80 degrees Celsius.

- The remaining cultures were placed back into the 37 degree incubator to grow overnight.

Conjugation
·Transform RP4 into S17-1 cells

Team:University_of_Washington/Notebook