Team:NTU-Singapore/Notebook/20 June 2008



  

=Friday 20 June= {|border="1" style="background-color:#ffffcc;" cellpadding="20"

Morning:

 * Miniprep for pLacI, followed by DNA concentrating using QiAQuick PCR purification kit.

Afternoon:

 * Transformation and cell cloning 4 empty plasmids from Biobrick registry:
 * pSB1A3
 * pSB1AC3
 * pSB1AK3
 * pSB1AT3
 * Transformation and cloning of LacI-GFP into LuxS knockout bacteria.
 * Make LsrA competent cells. Then transform 0.3 ul LacI-GFP plasmid into these cells.

Evening:

 * Gel extraction (using QiAQuick Gel extraction Kit)==>Ligation (using T4 DNA ligase) ==> Transformation for the following 4 samples:
 * E7 Insert - Empty plasmid vector (from GFP)
 * SupD Insert - Empty plasmid vector (from GFP)
 * T7ptag Insert - Empty plasmid vector (from GFP)
 * GFP insert - pFE vector (for characterization of Fe promoter)