User:University of Washington/26 August 2008

LuxR from AraC and TetR(Faifan)
ligation from gel extraction -cultures of plasmid construct transformation ended up growing on Kan plates after incubating overnight at 36 degree Celsius

-selected 3 colonies from each plate(LuxR, GFP) and grew overnight to do sequencing

new ligation with CIP reaction -miniprep I0462 x 3, ran gel and combined tubes

-restriction digest GFP and LuxR as in the table from yesterday(8/25)

-PCR purified digested promoter

-nanodropped the purified promoter: 261.9 ng/ul, 1.87(260/280), 2.04(260/230)

-did CIP reaction (Ingrid)
 * mix 10X CIP Buffer + 0.5 units of CIP per ul/DNA + DNA(have final concentration of 0.5 ul/10 ul)
 * incubated 37 degree Celsius 1 hour
 * stored at -20

MG1655Z1(Faifan)
-made glycerol stock of the strain. (non-resistance)

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