User:University of Washington/8 September 2008

LuxR from AraC and TetR(Faifan)
-Sequences of LuxR construct came back. 4/8 contained P1010, the other 4/8 had too many N's-cannot determine.

-Screening 14 colonies by colony PCR
 * resuspended each colony into 50 ul dH2O, vortexed
 * mix (10 ul rxn)
 * 5.95 ul dH2O
 * 1 ul 10x Taq Buffer
 * 0.25 ul 10mM dNTPs
 * 0.50 ul MgCl2
 * 0.6 ul VF2(10 pmol/ul)
 * 0.6 ul VR(10 pmol/ul)
 * 0.1 ul Taq
 * 1 ul colony suspension
 * Thermocycling
 * 1 cycle : 95 C : 6 mins
 * 35 cycle: 95 C : 30 s
 * ________: 53 C : 30 s
 * ________: 72 C : 2 mins
 * 1 cycle : 72 C : 10 mins
 * 1 cycle : 4 C : forever

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