Team:Hawaii/Notebook/2008-08- 9

= Things we did today =

Plasmid prep

 * Grace


 * Plasmid prep of E0240, J32007, I14032 ('05 and '08 distributions), I51020
 * Centrifuged for 90 min. at 4C after adding KoAc

Cryostock

 * Grace


 * Did not take out 2.4 ml of E. coli before doing plasmid prep (oops). Reinoculated for cryostocking tomorrow.

Construction of p+r+g, g+t, J33207+t

 * Grace


 * RE digest of nir+rbs, plac+rbs, GFP, B0015, J33207
 * Digested nir+rbs, plac+rbs, J33207 with EcoRI and SpeI
 * Digested GFP, B0015 with Pst1 and XbaI
 * Ran digests on EtBr stained 2% agaorse gel
 * Gel did not run despite using new TAE buffer
 * Redid RE digest overnight

Made KoAc solution for plasmid preps

 * Grace

Gel Purification

 * Margaret


 * Ran the large-scale PCR of aadA(BB), aadA(pRL1383a), and P1 lytic region on a gel, cut it out, and purified it using the Qiagen Kit

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"