Team:Hawaii/Meeting/2008-05-29

Agenda

 * 1) Team Agreement (Gernot)
 * 2) Presentations:
 * 3) BioBricks "Other" (GK)
 * 4) Plasmid (MR/NW)
 * 5) Plan Part B
 * 6) Margaret: cyan red dual plasmid system
 * 7) Grace: lux operon night light
 * 8) Krystle: bacterial export system, soluble proteins for crystallagraphy
 * 9) Team Photo
 * 10) After 9-10am meeting, Jamie will show the lab safety, chemical storage, etc walkthrough.

Minutes
Present: GK, KS, MR, SC, GP, KS, NW


 * 1) Presentations:
 * 2) [[Image:Biobrick_parts_other.pdf | BioBricks Other]] (GK)
 * 3) Other Parts (GK): Construction Intermediates, PCR Primers, Tags & Modifiers, Other Other Parts, Deleted Parts, Bacteriophate T7 Phage Parts & RNA Stabilizers.
 * 4) Plasmid (MR/NW)
 * 5) RSF1010 Overview: OriT, tra, mob overview
 * 6) Proposed design, 4 BioBrick subcomponents
 * 7) RNA Stability questions (regions past coding seq, how far do we PCR past carboxy terminus for possible RNA stability features? Are there any?)
 * 8) Discussion regarding its usefulness (will be used by a lot of people)
 * 9) Plan Part B
 * 10) Margaret: cyan red dual plasmid system
 * 11) Grace: lux operon night light
 * 12) Krystle: bacterial export system, soluble proteins for crystallagraphy
 * 13) Team Photo
 * 14) Jamie showed us around the lab safety, talked about chemical storage, etc walkthrough.

Action Items

 * MR write up formal proposal with details on synthetic plasmid
 * E. coli grown and frozen, possible competent cell made
 * get autoclave trained