Team:Hawaii/Notebook/2008-09-11

= Things we did today =

Construction of p+r + s(+gf)

 * Grace


 * Ran RE digests from last night on gel
 * Extracted DNA from gel
 * slr1, pilA, nir+rbs
 * (From yesterday) slr1+GFPf, pilA+GFPf (8/12 and 8/13 transformations)
 * Ligated:
 * pSB1A3 and nir+rbs with slr1, pilA, slr1+GFPf, pilA+GFPf (8/12 and 8/13 transformations)
 * nir+rbs (on SpeI/PstI digested pSB1A2 plasmid) with slr1, pilA, slr1+GFPf, pilA+GFPf (8/12 and 8/13 transformations)
 * plac+rbs (on SpeI/PstI digested pSB1A2 plasmid) with slr1, pilA, slr1+GFPf, pilA+GFPf (8/12 and 8/13 transformations)
 * Transformed into DH5&alpha; (3A ligations) or DB3.1 (2-way ligations) cells

Restriction digest

 * Krystle
 * 20ul reactions:
 * RE digest 3 versions of GFPf+tt with XbaI + PstI
 * RE digest rbs+GFP with EcoRI + SpeI

Plasmid Prep

 * Krystle
 * 5ml plasmid preps of nir, pilA, slr1 and slr2

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"