Team:Warsaw/Calendar-Main/5 June 2008

Preparation of pMPMT5+T7 construct Piotr, Weronika   Isolation of hypothetical pMPMT5 with T7 RNA polymerase.

Control digest of pMPMT5 + T7 RNA-polymerase with EcoRI (EcoRI buffer).  Gel electrophoresis.</li>

 Choice of proper clone and preparation for sequencing.</li>

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Preparation of pMPMT5-AID+AIDT7</a> construct Michał K. Inoculation of colonies of transformants (pMPMT5-AID with AID-T7 RNA-polymerase translation fusion) into liquid LB+tetracycline.

Blue/white and rifampicin test #1 Michał L., Ewa, Marcin, Piotr <ol>

Inoculation of of E. coli TOP10</a> with pZC320</a> into liquid LB broth (with 30 μg/ml of ampicillin).</li>

Design of primers for sequencing β-galactosidase from pZC320</a>. Primers:

pZCseqL</a> and pZCseqR</a></li></ol>