Brown: Team Resistance/25 September 2008

25 September 2008

 * Growth seen in 3:1 and 6:1 ligations!
 * Ligation cultures miniprepped.


 * 3:1 Restriction Digest (X2)
 * 1) 1 ul EcoRI
 * 2) 1 ul HindIII
 * 3) 5 ul Buffer E
 * 4) .5 ul BSA
 * 5) 10 ul DNA
 * 6) 32.5 ul sterilized water


 * 6:1 Restriction Digest (X2)
 * 1) 1 ul EcoRI
 * 2) 1 ul HindIII
 * 3) 5 ul Buffer E
 * 4) .5 ul BSA
 * 5) 9 ul DNA
 * 6) 33.5 ul sterilized water