Team:Rice University/Notebook/18 July 2008

=Friday, July 18th= 
 * David Ouyang
 * Miniprep R0040 (ptet)
 * Digest Amber Suppressor and ptet
 * Ligate together
 * Do ARFP mutant quikchange and DPN1
 * Transform ARFP and Amber Suppressor supposed
 * Taylor Stevenson
 * Repeat PCR from yesterday using these primers (NOTE: to view primers download this link and change extension to .doc (word file)).
 * tried raising annealing temperature to 58*C
 * 100bp standard
 * BleoC PCR product
 * Stf PCR product
 * Ligation of yesterdays digested and CIPed PCR primers
 * 1kb standard
 * Result-The bands at 750bp and 2.5kbp correspond to the PCR products of BleoC_AvrII and Stf_NotI. These bands were cleaned, concentrated, and digested with NotI.
 * The BleoC_AvrII digest was treated with CIP.
 * Digested DNA was cleaned and concentrated, and ligated overnight at room temperature.