Team:Warsaw/Calendar-Main/22 July 2008

Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA Paweł Isolation of plasmids from cultures inocluated on previous day. Control digest of isolated plasmids with NdeI and NotI (Orange buffer). Zero positive results obtained - just empty vectors. Another overnight ligation of pET15b-OmpA-omega (NdeI/NotI cut) with protein Z DNA.</li> </ol>

Cloning omega-A fusion on pKS</a> (second attempt) Michał L., Ewa, Marcin We have obtained white colonies of transformants and inoculated 10 of them on liquid LB+Amp100.

Cloning of protein A DNA to OmpA constructs Michał K.  <ol> Colony PCR</a> on colonies from plates with transformations pACYC177+OmpA_A_omega</a> and pACYC177+OmpA_A_alpha</a>. Primers used:

AL+SacI</a> and AP+NotI.</a> </li> Gel electrophoresis (Fig. 1.</a>).</li>  Confirmed transformant colonies (found only on pACYC177+OmpA_A_alpha</a> plate) inoculated to liquid LB with kanamycin. </li></ol>

<img src="http://2008.igem.org/wiki/images/1/1f/Ompa_ompa.jpg" width=340/></a> Fig. 1. Colony PCR on transformants with pACYC177_OmpA_alpha_A Upper and lower: 1. Marker 2-13. colony PCR (pACYC177_OmpA_alpha_A)