Team:Warsaw/Calendar-Main/3 July 2008

Change of the reporter from pZC320 with B-galactosidase to GFP or RFP Piotr, Weronika  There were blue and white colonies but no red or green fluorescence in UV.

Inoculation of liquid LB medium + Amp with white colonies.



Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega Paweł Colonies from each transformant strain from previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega) were inoculated to liquid LB + ampicillin.

Cloning alpha-A and omega-A fusions on pKS Michał L., Ewa, Marcin We have received sequencing results: A is OK but in case of Alpha-A and Omega-A the result is weird. It seems that there were error in synthesis of OmegaP+link10+homo2 and AlphaP+link10+homo2 primers which causes frameshift and cloning of these fusions has to be repeated from the beginning.