Team:Hawaii/Notebook/2008-06-30

= Things we did today =

PCR

 * Margaret


 * Ran 10 &mu;l PCRs for oriV, omega, rep, and mob

Purified I14032

 * Grace


 * Plate had incubated ~60 hrs. 48 colonies observed (satellites? kanamycin broke down?). Restreaked on fresh LB+kan50 plate.

Grew transformed E. coli for cryostocking

 * Grace


 * Inoculated 5 ml LB+amp100 with one colony of each of last week's Biobrick transformed E. coli
 * Incubated overnight at 37C with shaking (150 rpm)

Primers

 * Margaret, Grace, and Norman


 * Worked on redesigning omega cassette primers
 * Designed primers to verify rbs and tt
 * Reviewed primers, submitted for ordering

Secretion project timeline

 * Grace


 * Reviewed timeline with Dr. Presting
 * GP suggested that we create a flowchart

= Discussion =

= Quote of the Day =