Team:Edinbrugh/Results/Glycogen 4

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Glycogen Assay 4 (Quantitative: Raman)
Raman spectroscopy was performed by Dr Rabah Mouras.

Experiment Design
See Glycogen Assay 3 for explanation.

Legend

Organisms:
 * Sample 1: Control strain of E. coli containing a plasmid that should have no effect on glycogen levels.
 * Sample 2: E. coli overexpressing glgC.
 * Sample 3: E. coli overexpressing glgC16.

Growth Conditions:
 * Condition A: normal growth medium (LB, or L-broth).
 * Condition B: LB + 10 mM glucose.
 * Condition C: LB + 25 mM glucose.
 * Condition D: LB + 50 mM glucose.

It is expected that the presence of higher levels of glucose, up to a point, will increase the amount of glycogen produced by the cells.

Results


Analysis forthcoming.