Team:Michigan/9 October 2008

{|class="wikitable" border="0" cellpadding="10" cellspacing="1" style="padding: 1px; background-color:dodgerblue; border: 1px solid mediumblue; text-align:center" !width="10%" align="left" valign="top" style="background:gold; color:black"|

<< Previous Entry      Next Entry >>

=October 9, 2008=

 Why can't E.coli metabolize hot dogs? I'm afraid they just can't "digest" them 

 * PCR clean-up (quenches digests) on our digests from yesterday
 * Attempted two ligations: pCLOCK1,nifA (NdeI/SpeI) and BBa R0010 (lacI inducible lac promoter), nifA (R0010 with SpeI, nifA with XbaI/SpeI); transformed onto Ampicillin plates
 * Ran analytical gels on the digests too - all lengths matched up, except for our midi-prep of pCLOCK2; something messed up there, as the ladder in the lane next door didn't work either; need to re-check pCLOCK2


 * }

{|class="wikitable" border="0" cellpadding="10" cellspacing="1" style="padding: 1px; background-color:dodgerblue; border: 1px solid mediumblue; text-align:center" !width="10%" align="left" valign="top" style="background:gold; color:black"|

 Calendar

 * }