Team:Hawaii/Notebook/2008-06-14

= Things we did today =

Cell density test for PCC6803

 * Krystle


 * Plated 10-5, 10-7, 10-8 dilutions of PCC6803 (OD750 = .071 and 1.15) on BG-11
 * Plates in SC incubator

Glycerol stocked Biobrick colonies

 * Krystle


 * Combined 0.4ml 80% glycerol w/ 0.8ml of transformed E. coli grown overnight in LB + antibiotic
 * Cryostock could not be made for pUC18 because of contamination in overnight culture

Made competent cells

 * Grace
 * Same protocol as 6/2 competent cells
 * OD600 = 0.4567 (overnight culture)
 * OD600 = 1.1-1.2 (after CCMB80 added)

Triparental conjugation

 * Grace
 * Streaked BG-11+sp+sm plates with conjugates
 * Incubated in light & CO2 @ 30C in SC incubator

= Discussion = For competent cells, should cool eppendorf tray at 4C. After aliquot, transfer tubes to -80C EtOH tray and freeze at -80C. GP suggests we aliquot with tubes in dry ice.

Was Callahan lab hood working?

= Quote of the Day =
 * "Yeah, I'm trying to stave off the real world by staying in school a little longer." - KS


 * "Ooooooooooooooooooooooooh" -KS
 * "Yes Krystle, it's shiny." -GK