Utah State/3 July 2008

Fluorescence was tested for all of the GFP constructs. A notable difference was noted between the weak, medium, and strong promoter constructs. Excitation was 488 nm and emission tested at 509 nm. A peak outside of the excitation peak was noted in the media alone, and further investigation needs to be done to determine the emission source of this peak. Fluorescence will be tested again next week to see if fluorescence changes during stationary phase.