Newcastle University Wetlab/22 August 2008

Friday 22nd August

 * Stock pGFPrrnB from -80˚C was plated onto ampicillin, spectinomycin and normal agar (control) and incubated overnight at 37˚C.


 * 2μl pUC57-ncl08 and pJWV021 were run on gel to check quantity. This showed large quantities of both plasmids and therefore pUC57-ncl08 was restricted with EcoRI + NHeI and NHeI + BglII. Controls (buffer A and buffer M without enzyme) were used for both restrictions.