Newcastle University Wetlab/26 August 2008

Tuesday 26th August

 * Due to unforeseen circumstances, restriction mixtures were put in the fridge on Friday 22.08.08. Therefore the restriction was allowed to run for a further 1.5 hours before running on a gel.

Lane 1: 1kb ladder

Lane 2: empty

Lane 3: pUC57-ncl08 restricted with EcoRI + NHeI

Lane 4: pUC57-ncl08 restricted with NHeI and BglII

Lane 5: pUC57-ncl08 buffer A control

Lane 6: pUC57-ncl08 buffer M control

Lane 7: pure pJWV021



Results from overnight (ON) agar cultures:

- pGFPrrnB (amp) - no colonies

- pGFPrrnB (spec) - colonies present

- pGFPrrnB (no antibiotic) - colonies present


 * ON cultures were made:

- pGFP-rrnB grown on ampicillin

- pJWV021 grown on ampicillin

- pUC57-ncl08 grown on ampicillin