Team:ESBS-Strasbourg/28 July 2008

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WetLab
Sandra
 * Hybridization of the oligos for the linker sequence and the MCS
 * Blunting of the linker and Phenol-Chloroform-Precipitation

Paul -Digestion of lacI/lexA/tetR/gal4/plasmid death, by EcoR1 and Spe1 -gel migration and purification of those product -ligation overnight at 21°C

=>Cut with the wrong enzymes; now use of Spe1 and Xba1

General
Sandra Marius
 * Slides for presentation
 * Presentation (Layout,Assembly)