Team:NTU-Singapore/Notebook/2 July 2008



   =Wednesday 2 July= {|border="1" style="background-color:#ffffcc;" cellpadding="20"
 * Arrival of E7+Imm Oligo
 * PCR E7+Imm
 * Attempted Ligation of Fe-GFP & LacI-Rbs34
 * Failed Attempt due to faulty gel
 * Loading dye was unable to keep DNA sample settled at the bottom of the well
 * Samples settled into the gel and started floating out of it.
 * Preliminary Speculation of problem is due to loading dye.
 * Preliminary Speculation of problem is due to loading dye.

Luchao, Hung

 * miniprep for pFe,pLacI,GFP and RBS 34
 * Transformation and cloning for BBa_I763004 LacI-GFP and BBa_J04450 LacI-RFP. (the transformation on Tuesday was uncessful possibly because of the old agar plates).