Team:Warsaw/Calendar-Main/19 June 2008

Preparation of constructs: OmpA_alpha and OmpA_omega Michał K.

Inoculation of separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin).

Blue/white and rifampicin test #2 Piotr, Weronika  The analysis of lacZ sequence from pZC320: There is no mutation in beta-galactosidase gene. Why are the colonies white? Colony PCR.

Template: DNA isolated from white colonies

Primers: pZCseqL and pZCseqR</ol>