Newcastle University Wetlab/21 August 2008

Thursday 21st August

 * pJWV021 and pUC57-ncl08 were restricted using a different method:

pJWV021 stock
 * 48μl MillQ H2O
 * 8μl buffer M
 * 20μl pJWV021

- Tube 1 = 19μl stock solution + 1μl NheI + 1μl BglII

- Tube 2 = 19μl stock solution + 1μl NheI

- Tube 3 = 19μl stock solution + 1μl BglII

- Tube 4 = 19μl stock solution

pJWV021 and pUC57-ncl08 were restricted using a different method:

pJWV021 stock
 * 48μl MillQ H2O
 * 8μl buffer M
 * 20μl pJWV021

- Tube 5 = 19μl stock solution + 1μl NheI + 1μl BglII

- Tube 6 = 19μl stock solution + 1μl NheI

- Tube 7 = 19μl stock solution + 1μl BglII

- Tube 8 = 19μl stock solution