Team:Hawaii/Notebook/2008-10-27

= Things we did today =

Triparental conjugation

 * Grace


 * Checked on triparental conjugation cultures from 10/13. No growth was observed in any of the cultures.
 * E. coli were grown at too low antibiotic concentration (sp2.5). Cells probably lost plasmid during growth, before conjugation. Will repeat triparental conjugation on Wednesday.

Plasmid prep of BBpRL1383a

 * Grace


 * Used Qiaprep Spin Miniprep Kit to prep the rest of the BBpRL1383a liquid cultures.

Cryostocked BBpRL1383a

 * Grace


 * Cryostocked 10/22 culture of DH5&alpha; w/ BBpRL1383a

Restriction digest

 * Grace
 * 6 hour XbaI/SpeI digest (for pretty picture):
 * nir, nir+rbs, slr, pilA, slr+GFPf, GFPf
 * Overnight HindIII/BamHI digest:
 * J33207

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"