Team:University of Ottawa/23 July 2008

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Today in the lab
Dan
 * PCR amplification of T123
 * <li> I wanted to do PCR amplification of 0A and 0B, however PCR blocks were full, I will do this tomorrow
 * <li> 6 tubes of PCR reaction were prepared for T123, hopefuly this will give me the amount of DNA that I need for a successful ligation.
 * Transformation of p2S and p2D
 * <li>p2S and p2D were transformed in E. coli

Chris
 * Minipreparation of AtCRE
 * <li> Used minprep kit and protocol to isolate AtCRE from inoculated cells.
 * <li> Measured absorbance of resulting DNA samples to determine concentrations
 * Confirmation of AtCRE
 * <li> Performed a digestion of AtCRE with EcoRI against a water control and a positive control (DQ2325601 digested with EcoRI)
 * <li> Ran product on a 1% gel for 40 minutes at 80V
 * <li> Two of six samples showed desired bands.

Matt
 * Ligation of PTP2 with pSSA42
 * <li>Attempted another ligation of PTP2/pSSA42 this time with 1:1, 3:1, Vector with ligase, H2O, Vector without ligase.