Team:NTU-Singapore/Notebook/14 July 2008



  

=Monday 14 July= {|border="1" style="background-color:#ffffcc;" cellpadding="20"
 * LacI-GFP COLONIES!!
 * Miniprep of 18 colonies picked and one for T7ptag
 * Gel check was ran - 5 colonies had properly ligated LacI-GFP (got 1.1kb band), but T7ptag was not ligated well.
 * Gel check was ran - 5 colonies had properly ligated LacI-GFP (got 1.1kb band), but T7ptag was not ligated well.

Well Done!
 * Ligation of Fe and GFP was also carried out.
 * pFe insert from last Friday (stored at -20). We also did nanodrops before ligating to measure the concentration.
 * GFP vector also from last Friday digestion.
 * This time, we used 2 insert:vector ratios (1:3 and 1:6).