Team:The University of Alberta/24 July 2008

General

 * Did mini-preps of J61003 because we were running out
 * Digested BisDA/B with single digests using AgeI and NgoMIV to test the enzymes because weve been getting cruddy digests when using both of them
 * UPDATE: It would appear that AgeI is cutting but NgoMIV isn't. Could be a buffer issue?

System 2
Today we have taken the ligation from yesterday and transformed it as well as digested it.
 * if all goes well the digested product should be B0034+TETR
 * Column Purified the digested product.
 * Then ligated the B0034+TETR with LacIQERE giving us a complete system 2.
 * PCRed the digested product i.e tetR+BOO34
 * Digested pTet with Spe/Pst. Column purified the digested product.
 * Digested RFP with Xba/Pst.

System 3

 * Digested 5ul of J61003 with Xba/Pst; this will let us insert the Tryp biobrick digested and ligated yesterday
 * Ran the digested J6 on a gel; gel purified