Newcastle University Wetlab/20 August 2008

Wednesday 20th August

 * The dilute 2.2kb fragment was concentrated by repeated rounds of heating and cooling. This was purified to see if any plasmid had been obtained. As the sample was in 500μl buffer, it was split into 5 x 100μl aliquots. Each was purified separatly and then the samples pooled afterwards to maximise the yield of DNA. This was then run on a gel.


 * The gel showed no pGFPrrnB (restricted with EcoRI and NheI) and only a small amount of pGFPrrnB (restricted with EcoRI). Therefore pGFPrrnB and pUC57-ncl08 were restricted using EcoRI and NheI (as this also showed as absent on the gel).


 * Following these restrictions, the samples were run on gel which again showed them to be absent.