Team:Hawaii/PCC6803 cell density

Abstract
We would like to correlate OD750 with specific cell densities (# cells/ml)

Protocol

 * 1) Plated 200 &mu;l of 1, 10-1, 10-2, 10-5, 10-7, and 10-8 dilutions of PCC6803 on BG-11.
 * 2) Incubated at 37C with illumination in the SC lab for 12 days.

Results
Cell density was determined using the 10-5 colony count as it was the only statistically significant number (30<x<300).

Cell density for OD750=0.071 was approximately 2.40 x 107.

Cell density for OD750=1.159 was approximately 6.65 x 107.

Discussion


Reference: Richardson, D.L. et. Al., “Synechocystis PCC6803: a euryhaline cyanobacterium,” FEMS Microbiology Letters 18 (1983): 99-102.