Waterloo/18 October 2008

08/10/18 Saturday

[] kit prep Plac-rfp' - Inoculations seemed to have failed again [x] digest (digest 16 ul of a ~600-800 bp part in pSB1A2 so we can subclone into this vector) [x] gel

[x] GEx

[] run check gel - Didn't have time for the check gel

[x] ligation of all above into BioBrick vector subcloning - include ligation of GEx'd PCR products into pJET! - hour ligation in 37 plus left overnight on bench

What we've been calling Plac-rfp' actually has the promoter for lacIQ (i.e., a strong constitutive promoter)!! Hence its KmR property... We have to make R0011.B0034.E1010 still (rfp' controlled by the hybrid lambda promoter (strong promoter) with the LacI binding site)