Team:Rice University/Notebook/21 May 2008

=Wednesday 21 May=
 * Taylor Stevenson
 * Transformation From New Registry (Paper Punches)-Electroporation of BBa_I13602 located on pSB1A2 into TetR electrocompetent cells.
 * Added 2uL of DNA solution obtained by soaking DNA punch in (10:1) Tris:EDTA buffer incubated @ 42*C for 20min.
 * Electropulse parameters: 1.8 kV & 5.4 ms
 * Plated 100uL and 20uL of transformed cells as well as 20uL of 1/100 and 1/10,000 dilutions in sterile 50% glycerol.  Plates were cultured O/N.
 * Result-no colonies were observed at any dilution

