Newcastle University Wetlab/12 August 2008

Tuesday 12th August
- pGFPrrnB restricted with EcoRI + NheI
 * pGFPrrnB and pJWV021 were restricted using 10μl plasmid and 0.5μl each enzyme in 50μl total reaction volume:

- pJWV021 restricted with NheI then BglII in a 2 step reaction (the second step used 48μl purified plasmid in 100μl total reaction volume).

Running the samples on a gel showed only partial restriction had occurred and that there was very little pJWV021.