Team:ESBS-Strasbourg/10 July 2008

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DryLab

 * MM: Sponsoring
 * michi:
 * design of the oligos to construct the operons of our DNA Binding Proteins.
 * design of the promoter region: get CMV minimal promoter

WetLab

 * Katja:
 * Preparation of LB liquid media and LB(A) plates
 * Transformation (db3.1) with positive and negative control and BB_p1010 in Amipicillin and in Kanamycin backround
 * Sandra:
 * resuspension of primers to stock solutions of 50 µM
 * making primer aliquots of 10 µM (4x 50 µl)
 * transformation (dh5alpha cells) with positive and negative control and BioBrick GFP-device
 * MM:
 * TSB-Buffer
 * Made competent cells (TOP10, db3.1, dh5alpha)
 * Transformation (TOP10)

Quote of the day
MW: "I always got heavy beard growth -roarr"