Team:The University of Alberta/12 June 2008

Meeting At 4:00PM!

Today

 * Made up new Tet and Kan plates because the ones we made forever ago were made with 1/5 the correct amount of Antibiotics in them!
 * Set up O/N of Purple Russian, Blue Ox and Tryp in their original pUC57 plasmids.
 * Ran colony PCR of the colonies used above, to make sure that they are positive colonies with our genes. Results shown in image to the right.
 * David and Kelly gel extracted the products. Concentration was low for all three (~40ng/ul) but still higher than we have been getting previously. Digesting these will undoubtedly reduce our concentration even lower; prehaps we should just PCR from these and gel extract again.
 * Made 15ml of Kan and 100(!)ml of Amp - should last us a long time
 * The first Blue Ox we made was digested, ligated and transformed into I0500; transformants were streaked and placed into 37 degree incubator overnight.
 * Finished Westerns of the Butanol stuff. No result after 60 second exposure. They were left in the dark to expose overnight.

Lab Tip of the Day
Make sure you add the correct amount of antibiotics when making plates, otherwise we will have to do it all over again.