Team:Chiba/parts/BBa K084008



parts usage
BBa_K084008(plac+rbs+RhlI(no LVA))



日本語:IPTGにより、シュードモナス由来のC4HSL,C6HSL合成酵素,RhlIを発現するデバイス.

English:

Purpose
To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.

Method

 * 1) Transformed BBa_K084008 into E.coli strains(XL10GOLD or JW1908) and BBa_T9002 into E.coli strain(JW1908).
 * 2) Inoculated them independently in liquid media. Incubated at 37°C 12h
 * 3) Mixed them.
 * 4) Incubated at 30°C and 37°C.
 * 5) Measured intensity of green fluorescence at regular time intervals.