Team:Mexico-UNAM-IPN/Horizontal-Transfer/1 October 2008

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TRANSFORMATION OF COMPETENT CELLS JM101

Eniak Hernández


 * Sample (9) BBa_1739201 low copy
 * Sample (11) PUC19 high copy


 * 1) Thaw the cells on ice for 5 minutes.
 * 2) Take 50µl of cells and add 5µl of DNA (samples 9 and 11).
 * 3) Incubate on Ice 20 min.
 * 4) Heat shock of 40-50 seconds in termoblock at 42°C. DO NOT AGITATE.
 * 5) Thaw on ice for 2 minutes.
 * 6) Add 950µl of sterile LB media.
 * 7) Incubate for 1.5hrs at 37°C at 15rpm.
 * 8) Plate in petri dishes with LB/amp, 100µl of media. First centrifugate (7min, 3500rpm) and disolve the pellet in 100µl of media, plate. Incubate at 37°C overnight.