Team:Hawaii/Notebook/2008-07-23

= Things we did today =

Gel for Plasmid Prep from Yesterday

 * Margaret



Biobrick Extraction

 * Margaret


 * extracted: I14032, pSB1A7, I51020, J23012 and transformed into DB3.1 cells. Plated on amp100. **the problem is that I14032 is Kan resistant. I introduced 1mM IPTG to the 2 hour 37&deg;C incubation, but we will not see the results of this because of my antibiotics mix-up
 * on 7/24 there were 2 colonies on the pSB1A7 plate and 4 colonies on the I51020 plate, these were restreaked
 * on 7/25 the re-streaked plates were placed in 4&deg;C.

Plasmid Prep pRL1383a-M

 * Krystle


 * 200 ml prep of the new pRL1383 plasmid with biobrick multiple cloning site

Checked for transformants from yesterday/restreaked

 * Krystle


 * GFP + tt = 5 colonies
 * GFP fusion + tt = 15 colonies
 * nir + rbs = 1 colony

= Discussion =

= Quote of the Day =