Imperial College/10 September 2008

=10 September 2008=

Growth Curve
We performed an OD600 against time growth curve for B. subtilis. The results are shown below:



Please click on the link below for the Excel data file: [[Media:OD 600 v Time.xls]]

Cloning

 * All ligations from yesterday failed, no indication of why. 2 test ligations set up and used to transfom Xl1-blue. *Overnight ligations set up for transformation tomorrow
 * Constructs 3, 6, 10 and 12 midiprepped

Motility
Took 20 videos, binned x4 to a frame rate of 27.9fps, more than twice the rate at which previous videos were taken. However, video quality is not as good as before, but cells can still be easily distinguished.

Growth Curve
Obtained sigmoidal curve for bacteria growth using MATLAB.