Team:Warsaw/Calendar-Main/20 October 2008

Preparation of alpha_linker under PT7 (BBa_K103019) Michał K. Inoculation of colonies from plate with ligation of pSB2K3 + alpha_linker under PT7 (BBa_K103019) to LB with kanamycin.

Preparation of AID under pBAD/araC (BBa_K103002) Michał K. Inoculation of colonies from plate with ligation of pSB1A3 + AID under pBAD/araC (BBa_K103002) to LB with ampicillin.

Preparation of OmpA_linker_alpha_linker (BBa_K103014) Michał K., Piotr 

Digest</a> of pSB2K3</a> + OmpA_linker_alpha_linker under Plac (BBa_K103017)</a> and OmpA-linker (BBa_K103006)</a> with EcoRI and SacI (BamHI buffer). </li> <li>Gel electrophoresis and gel-out</a> of proper band 500 bp for OmpA-linker (BBa_K103006)</a> lane and 3000 bp for PSB2K3 (+ alpha linker)</a> lane. Fig. 1</a>. </li> <li>Ligation</a> of isolated DNA fragments.</li>

<li> Transformation</a> of TOP10</a> with above ligation.</li>

<li> Tranformants plating on LB with kanamycin. </li>

<img src="http://2008.igem.org/wiki/images/5/58/Go_20_10_2008.jpg"></a> Fig. 1.EcoRI/SacI digests of plasmids pSB2K3 + OmpA_linker_alpha_linker under Plac (BBa_K103017) and OmpA_linker (BBa_K103006): 1. Marker 2.EcoRI/SacI digest of pSB2K3+OmpA_linker (BBa_K103006) 3.EcoRI/SacI digest of pSB2K3+OmpA_linker_alpha_linker under Plac (BBa_K103017)

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