Rensselaer/8 August 2008

Promoter Group

'''Transformation of Ligation '''

Thaw TOP10 supercompetent cells on ice.

Add 5 ul of Fe/mRFP ligation. Tap tube to mix.

Incubate on ice 30'.

Heat shock cells @ 42C for 45''.

Incubate on ice 2'.

Transfer cells + DNA to 1.5 ml tube w/ 900 ul 2XYT.

Incubate 1h @ 37C w/ shaking.

Plate 100 ul cells undiluted and 100 ul cells concentrated.