Team:Hawaii/Notebook/2008-08-26

= Things we did today =

Ran RE digests on gel

 * Grace


 * Gel did not resolve. Bands were convex, poor separation between bands. Bad buffer?
 * Redid RE digests from yesterday
 * Used PCR products of nir and J33207

Inoculated for plasmid prep

 * Grace


 * BB-pRL1383a and J33207 in TB+amp100

Gel Purified

 * Krystle


 * Ran overnight ligation products on a 3% agarose gel
 * discussion: If we decide to do the 3A in two parts, we will have to re-restriction digest the first part before moving on

Transformation of ligated GFPf + TT

 * Krystle


 * Performed transformation protocol with DB3.1 and GFPf + tt ligation from 08/25

Ligation
Margaret
 * Overnight in 4&deg;C
 * rep+pSB1A3 (1:1)
 * rep+pSB1A3 (1:3)
 * rep+pSB1A3 (1:6)
 * P1 lytic +pSB1A3 (1:1)
 * P1 lytic +pSB1A3 (1:3)
 * P1 lytic +pSB1A3 (1:6)
 * [Plac B0030]+pSB1A3 (1:3)
 * [Plac B0030]+pSB1A3 (1:6)
 * [Plac B0034]+pSB1A3 (1:6)**ran out of vector

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"