Team:Chiba/Calendar-Home/22 August 2008

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21 August 2008 <|> 23 August 2008

Team:Input
Mini prep: Minipreped the cultures(<--21/8).


 * BBa_R0051(2007)
 * BBa_R0051(2006)
 * BBa_J06650(2007)
 * BBa_J06650(2006)
 * BBa_J22136(2007)
 * BBa_J22141(2007)

insert check:Digestion


 * 1) BBa_J06650(2006)
 * 2) BBa_J06650(2007)
 * 3) BBa_J22136(2007)
 * 4) BBa_J22141(2007)
 * 5) BBa_R0051(2006)
 * 6) BBa_R0051(2007)

UV irradiation test

o -Ptrc-LuxR-Plux-cI-colE1-Amp- o -PcI-GFP-p15a-Cm-(JW1908)
 * two plasmids from
 * 1) Incubated cultures(from Glycerol Stocks) with 2mL of LB-Ampicillin, Chloramphenicol Medium and LB-Ampicilline, Chloramphenicol and AHL100nm Medium for 12 hours at 37 degree.
 * 2) moved the cultures to small plates and started UV-irradiation. (wavelength:254nm,distance from the UV lamp to the cultures were 8cm.)
 * 3) After exposing both plates to UV light for 15min, 4h, 8h, or 12h, we took 20ul from each plate, diluted 105-fold, and plated 20ul of the resulting solution t (LB-Amp,Cm)(LB-Amp,cm,AHL100nM) plates.

The number of colonies aftetr culturing at 37&deg;C for 12h.  result  GFP fluorescence was observed from plates without AHL but not from plates containing 100nM AHL.

Team:Communication

 * Transformation
 * competent cells : XL10G
 * BBa_J13002(2007)
 * BBa_J13002(2006)
 * BBa_J04500(2007)
 * BBa_J04500(2006)
 * (Locate & 2 Punch ~iyama ver.~)
 * BBa_C0076(2008) -> No colonies on plates
 * BBa_C0077(2008)　-> No colonies on plates
 * BBa_C0078(2008) -> No colonies on plates
 * BBa_J04500(2008) -> No colonies on plates

--->(24/8)Mini prep

Team:Output
Mini prep
 * BBa_J52008
 * BBa_F2620
 * BBa_T9002
 * BBa_J33202
 * PUC19
 * PGEX VENUS YFP(GST fusion)

Transformation
 * PGEX VENUS YFP(GST fusion)