Team:Hawaii/Notebook/2008-05-24

= Things we did today =

Made seed stocks

 * Margaret
 * five 10mL seed stocks of PCC6803 in BG-11 media
 * five plates smeared with 200uL PCC6803 in BG-11 media, all from old plates

Clumping Experiment

 * Margaret

Hypothesis: Liquid Cultures of PCC6803 clump when incubated in Erlenmeyer Flasks and does not when cultured in 'box' culture flasks: Is this due to the motion of the media in different containers, the surface area of the culture, is it reproducible?
 * Question 1: Does the clump go back to homogeneous when placed in square culture flask?
 * Test: Took 0.5mL of a clump and placed it in 2 square culture bottles. (1 of the bottles was fresh BG-11 media, the other was the old stock to which buffer was added today)
 * Compare to: Took 0.5mL of a homogeneous liquid culture and placed it in 2 square culture bottles. (1 of the bottles was fresh BG-11 media, the other 1 was the old stock to which buffer was added today)
 * Question 2:Will previously homogeneous culture go to clump?
 * Homogeneous liquid culture placed in BG-11 media in an Erlenmeyer flask 1:10, Vfinal=50 mL
 * Homogeneous liquid culture placed in BG-11 media in an Culture flask 1:10, Vfinal=50 mL



Discussion
"History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"
 * 1) What to do in the future:
 * 2) The plates are drying out too soon so we need to make them 5mm thick instead of 3mm thick.
 * 3) Get autoclave certification. We need to make media and autoclave hood materials: paper towels (?), eppendorf tubes, blocks, etc.
 * 4) Get Pictures!
 * If our action is not recorded, we are doomed to either repeat the error, or fail to replicate the success.

Update on Seed Stocks

 * The seed stocks are forming a nice lawn on the media

Update on Clumping

 * The homogeneous culture is now clumping. Clumps are reverting to homogeneous

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