Team:Chiba/Calendar-Home/19 September 2008

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18 September 2008 <|> 20 September 2008

Team:Communication
-->(?/9) Gel Check

R0010 (Plac)

C0161 (RBS+Lux)

C0261 (Lux+LVA)

--->Gel Extract

(3 wells for 1 sample)
 * note:ADB buffer,
 * left at 37&deg;C,for 6 hours
 * --->Zymo Clean


 * elution
 * BBa_J04500-->27&mu;L
 * BBa_C0161-->11&mu;L

--->Gel Check


 * --->Ligation


 * --->Transformed above ligation onto a LB-amp plate.


 * note
 * competent cell:XL10GOLD
 * DNA:1&mu;L,SOC:120&mu;L

--->(20/9)'''Mini prep


 * time-delay test
 * conditions
 * 1) strain Senders:XL10GOLD Receiver:JW1908
 * 2) temparature

30&deg;C and Room Temparature

Team:Output
colony PCR insert:BBa_S03119(&times;16)(marker:Kan) insert:K084004(&times;5)(marker:Kan) insert:K084005(&times;7)(marker:Kan) insert:K084006(&times;5)(marker:Kan)