Team:The University of Alberta/21 July 2008

Today

 * Transformed the ligation of Tryp into J6
 * Finished Westerns of the Butanol suff in J6...RESULTS PENDING
 * Results in. Didn't work. Again. Either we're doing the Westerns incorrectly or the butanol parts aren't being translated for some reason.
 * Worked out the procedure for putting together all of our parts.
 * Re-digesting Lac1 ERE, TetR. RBS.6x His, RBS-6xHis, TDNA-MCS with Xba/Pst.
 * Digested BisDA and BisDB with Xba/Pst. Ran them on gel.
 * They looked good, so gel purified the bands.
 * Cut I0500 with Spe and Pst. No bands appeared on gel. Reran them on gel and got two bands.
 * Set up the O/N's using the colonies on plate. Minipreps to be done on Tuesday.