Team:Hawaii/Notebook/2008-08-17

= Things we did today =

Verification of transformants

 * Grace


 * X-gal did not work?
 * Colony PCR to verify insert
 * ~320bp band is due to pRL1383a plasmid
 * BB-pRL1383a + J33207 bands are smaller than (-) control bands, potential success in replacing GFP w/ lac device
 * (-) bands due to contamination
 * Restreaked colonies to purify
 * Grew up colonies in TB+sp100 liquid media for plasmid prep on Tuesday

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"