Team:Chiba/protocol/Time Delay Test

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Time Delay Test


 * 1) Transformed sender and receiver into E coli strains.
 * 2) Inoculated them independently in liquid media. Incubated at 37℃ 12h.
 * 3) Inoculated again in Fresh liquid media upto about OD600=2 at 37℃
 * 4) Washed sender and receiver.
 * 5) Mixed them. (Sender:Receiver=1000μL:1000μL)
 * 6) Incubated at 25°C, 30°C　or 37°C.
 * 7) Measured intensity of green fluorescence at regular time intervals.(Fluoroskan AscentR FL＆Fluoroskan AscentR　Thermo ELECTRON CORPORATION)