Team:Warsaw/Calendar-Main/12 August 2008

Checking for presence of protein A on the cell membrane

Emilia Inoculation of pACYC177+OmpA_A_alpha, pACYC177+OmpA_Z_alpha and pACYC177+OmpA_omega_&Delta;A_alpha (with and without induction).

Purification of proteins: Z-alpha and Z-omega Piotr

Bacteria centrifuged and sonicated (according to protocol). Sonicate centrifuged.  Supernatant discarded, pellet resuspended in ddH20, centrifuged and again resuspended in ddH20. 