Team:Hawaii/Notebook/2008-10-14

= Things we did today =

Construction of secretion device (cont.)

 * Grace
 * Ran RE digests on gel
 * Extracted rgt1 and prpgt5 from gel
 * Ligated:
 * prpgt5 + BBpRL1383a-1
 * nir + rgt1 + BBpRL1383a-1
 * plac + rgt1 + BBpRL1383a-1
 * nrsg6 (from 10/11) + BBpRL1383a-1
 * J33207 + pRL1383a

Plasmid Prep

 * Krystle
 * Plasmid Prepped 2 cultures of BBpRL1383
 * Nanodrop concentration is 330 ng/ul
 * Gel verification for plasmid shows blank lanes

Sequencing analysis

 * Grace
 * prpgt, nrsg, sgt, and pgt sequences all returned inserts of E. coli genomic DNA instead of desired construct

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"