Team:Hawaii/Notebook/2008-08-23

= Things we did today =

Verification of transformants (cont.)

 * Grace


 * Plasmid prep of nir+rbs, rbs+GFP


 * PCR of insert of nir+rbs, nir (control), rbs+GFP, GFP (control)

3A assembly

 * Grace


 * Gel purified RE digests
 * nir band did not show up on gel
 * Ligated with pSB1A2:
 * plac+rbs
 * GFP+tt
 * GFPf+tt
 * Transformed DB3.1 cells with 5&mu;l ligation reaction

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"