Team:Hawaii/Notebook/2008-10-22

= Things we did today =

Plasmid prep of BB-pRL1383a

 * Grace


 * E. coli did not grow. Reinoculated.

Extracted J04430 from filter paper

 * Grace


 * Used 2 &mu;l of filter paper solution for PCR to verify plasmid
 * Something is wrong with the J04430 filter spot iGEM sent us. PCR with VF2/VR primers did NOT yield the expected band ~1000bp.

Transformation

 * Grace


 * Used 2 &mu;l each of BBpRL1383a-1 and J04430 (from filter) to transform DB3.1

Construction of GFP secretion device

 * Krystle
 * Gel purify restriction digests from yesterday
 * Ligate nir+rbs and slr+gfpf into restricted psB1A3 from Margaret
 * Transformed into DH5&alpha;

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"