Team:Hawaii/Notebook/2008-08-25

= Things we did today =

Ligation & Transformation

 * Margaret


 * ligation of rep+pSB1A3 and P1+pSB1A3, transformation into DH5alpha cells

Colony PCR
Margaret
 * verified the colonies from the (pLac+B0030) ligation and transformation. no transformants for the B0034 ligation

Verification of transformants (cont.)

 * Grace


 * PCR'd nir, nir+rbs, GFP, GFP+rbs
 * Ran on 3% agarose gel

Restriction digest

 * Grace


 * RE digested overnight:
 * BB-pRL1383a and J33207 with XbaI and PstI
 * C0012 with EcoRI and PstI (to isolate vector)
 * nir with EcoRI and SpeI

Ligation

 * Krystle


 * Ligated overnight:
 * plac + B0034 (both synthesized)
 * plac + B0030 (both synthesized)
 * gfpf (vector) + tt (synthesized)
 * gfpf (re'd with E-S) + tt (synthesized)

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"