Team:Hawaii/Notebook/2008-10-25

= Things we did today =

Verification of plasmid preps

 * Grace


 * PCR'd plasmid preps from yesterday
 * RE digested all preps using EcoRI and PstI for 3 hours
 * Ran on gel

Inoculated 50ml TB+sp100 with single colony BB1 for plasmid prep

 * Grace

Construction of BB-pRL1383a/K125820
Krystle
 * Restriction digest BB-pRL1383a with EcoRI and PstI
 * Dephosphorelated restriction product with SAP for 1 hour
 * Ligated cut/dephosphorelated product with gel purified K125820 using T4 ligase

iGEM presentation

 * Grace


 * Worked on presentation

parts

 * Margaret


 * added parts to registry

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"