Team:ESBS-Strasbourg/11 July 2008

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DryLab
michi:
 * Planing of the measurements we will have to perform
 * Get started with IRES: WOW their exist cell cycle dependent IRESes

WetLab

 * results SD, MM and KK:
 * TOP10: 5* 10^5 cfu/ug
 * db3.1: 10^5 cfu/ug
 * dh5alpha: 10^6 cfu/ug
 * Primer
 * the arrived verification primer were resuspended to give a stock solution of 50 µM
 * aliquots of these primers (4x 10 µM) were produced and everything frozen (-20°C)
 * Transformation
 * competent Top10 cells from Invitrogen, protocol from Marias Lab: H2O (negative control), pxj (positive control), 5 BioBricks (EYFP, vector without insert, adh terminator, lacI, lamdba CI), plated on ampicillin
 * competent db3.1 cells, protocol from Barbaras Lab, DNA was already in KCM and cells were just added and plated, K3 on kanamycin and A2 on ampicillin
 * competent dh5alpha cells, protocol from iGEM using SOC medium: H2O (negative control), pxj (positive control), BioBrick (EYFP), plated on ampicillin

Quote of the day
"At least we have worked steril." (Marius)