Team:Chiba/jk/γ/week 5

実験ログ

14,September,2008
Time Responce Liquid Digestion test
 * BBa_Q04510
 * BBa_I716463
 * BBa_C0051
 * BBa_C0070
 * BBa_J06620
 * BBa_J22141
 * BBa_C0076
 * BBa_I716542
 * BBa_I716462
 * BBa_C0078
 * BBa_J01101
 * BBa_C0071
 * BBa_I712019
 * BBa_S01416
 * BBa_C0079
 * BBa_C0077
 * BBa_J23007
 * BBa_I7100(x2)
 * BBa_S01416

-->37℃ 1hour

Gel check

16,September,2008
Digestion test
 * BBa_R0040(ptet)+BBa_S03158(RBS+RhlR)(x2)
 * BBa_R0040(ptet)+BBa_S03156(RBS+lasR)(x5)
 * BBa_R0040(ptet)+Ba_S03160(RBS+cinR)(x2)
 * BBa_S03158(RBS+RhlR)
 * BBa_S03156(RBS+lasR)
 * Ba_S03160(RBS+cinR)

-->37℃ 1hour

Gel check

Transformation
 * BBa_J37019(LuxI+RBS+LuxR)->no colony
 * BBa_J37033(RBS+LuxR)
 * BBa_J37020(LuxI+LuxR+pLux+GFP)
 * BBa_J37032(pLux+GFP)

PCR
 * Venus YFP(ptet+LuxR+YFP)

17,September,2008
Transformation MINI Prep Digestion
 * BBa_I13500(RBS+GFP)
 * BBa_J37019(Luxpr+LuxR)-->no colony
 * BBa_J37033(RBS+LuxR)
 * BBa_J37020(Luxpr+LuxR+LuxR+GFP)
 * BBa_J37032(pLux+GFP)
 * BBa_I7100(ptetR+RBS)
 * BBa_S03119(ptet+LuxR)
 * Ligation product(ptet+LasR)
 * Ligation product(ptet+RhlR)
 * Ligation product(ptet+cinR)

-->37℃ 1hour ->Next day

18,September,2008
SAP 1μl ->37℃　1hour ->30min　65℃ Zymo ->25μl Ligation vector:BBa_I7100 insert:BBa_S03119(ptet+LuxR),Ligation product(ptet+LasR,ptet+RhlR,ptet+cinR)

TRANSFORMATION BBa_J54103(RBS+GFP) BBa_P2006

Gel check Ligation product(Venus YFP) BBa_J37033 BBa_J37020 BBa_J37032

19,September,2008
colony PCR ptet+LuxR(x16) ptet+LasR(x7) ptet+RhlR(x5) ptet+cinR(x5)

20,September,2008
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