Team:NTU-Singapore/Notebook/24 July 2008



  

=Thursday 24 July= {|border="1" style="background-color:#ffffcc;" cellpadding="20" Hung,Darius:
 * RESULT from Wednesday ligation and transformation:
 * E7-Term got 7 colonies!!
 * Lysis-standard vector and LsrA-standard vector got lots of colonies!!
 * Lysis-Term also got 5 colonies!!
 * Lysis-Term also got 5 colonies!!
 * Digestional gel check for Lysis-Terminator (with Lysis and Term. for comparision) again (this time, we used 2% gel and 100bp ladder). We tried digesting for 1 hour, and 2 hours. RESULT:
 * Digesting for 1 hour and 2 hour didn't make much differences.
 * All 3 Lysis-Term bands were actually just Term. So all the 3 colonies we obtained just contained the uncut Terminator. NOTE:
 * For 2% gel, we should run for at least 1 hour in order to see clear and distinct bands.
 * Terminator vector is 3kb. (as contain both AmpR and KanR), not 2kb as presumed.
 * Bluescript vector is 2.5kb.
 * Inoculation: of the colonies we obtained this morning.