TUDelft/6 October 2008

=October 6th=

Colony PCR
A new colony PCR was done on the transformed cells. The gel is depicted in figure 1. AtoB, idi, ispA and BB_K115033 are listed in the TUDelft registry. Sizes of the parts + biobrick primer DNA should be:
 * atoB: 1504
 * idi: 868
 * ispA: 1219
 * K115033: 1604



Growing thermoparts
For miniprepping we will grow the thermoparts K115030, K115033 and K115035 on Tetracycline. We decided not to grow a new K115032 as it didn't look any better as the one we're working with already.