Wisconsin: Lignin Project/1 July 2008



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The overnight PCR reaction failed. Used the purified PCR product from yesterday to set up a digestion (along with pBAD30) as described before. The digestion was verified on a gel and extracted using the Qiagen kit. This time we obtained enough insert to do a ligation but not enough vector. To solve this we grew 40mL of DH5a with pBAD30 overnight at 30C Team Fungus: Ran 1% agarsoe gel of last nights PCR with plasmid as template. Results were inconclusive. Ran yet another PCR. Used a touchdown PCR cycle. Running low on cDNA. Ran another digestion of purfied plasmids. Results inconclusive.
 * Team Sorbitol: