Team:NYMU-Taipei/Project/Phosphate/PRIMER of pstSCAB-phoU PST operon

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biobrick cutting sites scanning report

 * by serial cloner 1.3

>EG10734 pstS (complement(3909548..3904876) E. coli atgAAAGTTA TGCGTACCAC C GTCGCAACT GTTGTCGCCG CGACCTTATC GATGAGTGCT TTCTCTGTGT TTGCAGAAGC AAGCCTGACA GGTGCAGGTG CAACCTTCCC TGCGCCGGTG TATGCCAAAT GGGCTGACAC TTACCAGAAA GAAACCGGTA ATAAAGTTAA CTACCAGGGT ATCGGTTCTT CCGGTGGCGT AAAACAGATT ATCGCTAATA CCGTTGATTT TGGTGCCTCT GACGCGCCGC TGTCTGACGA AAAACTGGCT CAGGAAGGTC TGTTCCAGTT CCCGACCGTG ATTGGCGGCG TGGTGCTGGC GGTTAACATT CCAGGGCTGA AGTCTGGCGA ACTGGTGCTG GATGGTAAAA CCCTCGGCGA CATCTACCTG GGCAAAATCA AGAAGTGGGA TGATGAAGCC ATCGCCAAAC TGAATCCGGG TCTGAAACTG CCTTCACAAA ACATTGCTGT AGTACGCCGC GCAGATGGCT CCGGGACTTC CTTCGTCTTC ACCAGCTACC TGGCGAAAGT GAACGAAGAG TGGAAAAACA ACGTTGGTAC TGGCTCTACC GTAAAATGGC CGATCGGTCT GGGCGGTAAA GGTAACGACG GTATCGCCGC GTTCGTTCAG CGTCTGCCGG GTGCAATTGG TTATGTTGAA TATGCTTACG CGAAGCAGAA CAACCTGGCG TACACCAAAC TGATCTCCGC TGATGGTAAA CCGGTTAGTC CGACCGAAGA AAACTTCGCT AATGCAGCAA AAGGTGCAGA CTGGAGCAAA ACCTTCGCTC AGGATCTGAC CAACCAGAAA GGCGAAGATG CATGGCCTAT TACCTCTACC ACGTTCATTC TGATCCACAA AGATCAGAAG AAACCAGAAC AAGGCACAGA AGTGCTGAAA TTCTTCGACT GGGCGTACAA AACCGGGGCT AAACAGGCGA ACGACCTGGA TTACGCCAGC CTGCCGGATA GTGTAGTTGA ACAGGTTCGC GCTGCGTGGA AGACCAATAT TAAAGACAGT AGCGGTAAGC CGCTGTACTA A TAAAACTCC AGGCCGGGTA CGGTGTTTTA CGCCGCATCC GGCATTACAA AATGACTTTG TAAACGCGTT TAACTGAAGA GTAACTT ATG GCTGCAACCA AGCCTGCTTT TAACCCACCG GGTAAAAAGG GCGACATAAT TTTCAGCGTG CTGGTAAAAC TGGCGGCGCT GATTGTGCTA TTGATGTTGG GTGGCATTAT TGTCTCTCTG ATCATCTCCT CCTGGCCGAG CATTCAGAAA TTTGGTCTGG CTTTCCTATG GACCAAAGAG TGGGATGCAC CGAACGATAT CTACGGGGCG CTGGTGCCGA TCTACGGTAC GTTGGTGACT TCGTTTATCG CGCTGCTGAT CGCCGTCCCG GTGAGTTTCG GTATCGCCCT GTTCCTGACT GAGCTTGCGC CTGGCTGGCT GAAACGCCCG CTGGGTATCG CCATTGAGCT GCTGGCAGCC ATTCCAAGTA TCGTTTACGG CATGTGGGGC CTGTTTATCT TTGCGCCGCT GTTCGCCGTT TACTTTCAGG AGCCGGTCGG CAATATCATG TCGAATATCC CGATTGTTGG CGCGCTGTTC TCTGGCCCCG CATTTGGTAT CGGTATCCTC GCGGCAGGCG TGATCCTCGC CATCATGATT ATTCCGTACA TTGCGGCGGT AATGCGTGAT GTGTTCGAAC AAACCCCGGT GATGATGAAA GAGTCGGCCT ACGGTATTGG CTGCACCACC TGGGAAGTTA TCTGGCGTAT CGTTCTTCCG TTCACCAAAA ATGGTGTTAT CGGCGGCATC ATGCTGGGGC TGGGCCGCGC GCTCGGTGAA ACCATGGCGG TGACCTTTAT CATCGGTAAC ACCTACCAGC TCGACAGCGC CTCGCTGTAT ATGCCGGGCA ACAGTATCAC CTCTGCGCTG GCGAACGAAT TTGCGGAAGC GGAATCCGGT CTGCACGTTG CCGCACTGAT GGAACTGGGC CTGATCCTGT TTGTGATTAC CTTCATCGTC CTCGCCGCAT CGAAGTTTAT GATTATGCGC CTGGCTAAGA ATGAGGGGGC ACGCTA A TGG CTATGGTTGA AATGCAAACC ACTGCGGCGC TGGCTGAATC TCGCCGCAAA ATGCAGGCGC GTCGCCGCCT CAAAAACCGT ATTGCGCTGA CGCTCTCGAT GGCGACGATG GCCTTCGGCC TGTTCTGGCT GATCTGGATT TTAATGTCCA CCATCACTCG CGGTATCGAC GGTATGTCGC TGGCGCTGTT CACTGAAATG ACGCCGCCGC CCAATACGGA AGGTGGTGGT CTGGCGAACG CTCTGGCGGG TAGCGGGCTG TTAATTTTGT GGGCCACGGT ATTCGGTACG CCGCTGGGCA TTATGGCGGG GATTTATCTG GCGGAATATG GTCGTAAATC CTGGCTGGCA GAAGTGATTC GCTTCATTAA CGACATTCTG CTCTCTGCGC CGTCGATTGT GGTTGGTCTG TTTGTTTACA CCATTGTGGT GGCGCAGATG GAGCACTTCT CCGGCTGGGC GGGCGTGATT GCCCTGGCGT TGTTGCAGGT GCCGATTGTT ATCCGCACCA CCGAGAACAT GCTGAAACTG GTGCCGTACA GCCTGCGTGA AGCGGCTTAT GCGCTGGGTA CACCGAAGTG GAAGATGATC TCTGCGATTA CGCTGAAAGC GTCGGTGTCC GGGATTATGA CCGGTATCCT GCTGGCGATT GCCCGTATTG CTGGTGAAAC CGCGCCGCTG CTGTTTACCG CGCTCTCCAA CCAGTTCTGG AGCACGGACA TGATGCAGCC GATCGCCAAC CTGCCGGTGA CGATCTTTAA GTTTGCGATG AGCCCGTTTG CGGAATGGCA GCAATTGGCC TGGGCCGGGG TATTGATCAT TACCCTGTGC GTACTGCTGC TGAACATTCT GGCGCGCGTT GTTTTTGCGA AGAATAAACA CGGTTGA TAT TGCTGACACG GTTTTCCCCT CACCCTAACC CTCTCCCCAG AGGGGCGAGG GGACCGACCG AGCGCCTTTT TGACTCTGTA CACGGTTAAC ACTTTGCCGG ATGCGGCGTG AACGCCTGAT CCGGCCTACG GTAAGCCTGA TTAGCGAAGT GCATCAGGCA CGATGAGGAA AAGATTGCA A TGAGTATGGT TGAAACTGCC CCGAGTAAAA TTCAGGTTCG TAATTTGAAC TTCTACTACG GCAAATTCCA TGCCCTGAAA AACATCAACC TGGATATCGC TAAAAACCAG GTAACGGCGT TTATCGGGCC GTCCGGCTGC GGTAAATCGA CGCTGCTGCG TACCTTCAAC AAAATGTTTG AACTGTACCC GGAGCAGCGT GCGGAAGGTG AAATTCTGCT TGATGGCGAC AACATCCTGA CCAACTCTCA GGATATCGCA CTGCTGCGTG CGAAAGTGGG CATGGTGTTC CAGAAACCGA CGCCGTTTCC GATGTCCATC TACGACAACA TCGCTTTTGG CGTTCGTCTG TTTGAGAAGC TCTCCCGTGC CGACATGGAC GAGCGCGTGC AGTGGGCATT GACCAAAGCC GCATTGTGGA ACGAAACCAA AGATAAATTG CACCAGAGCG GTTACTCTCT CTCTGGTGGT CAGCAACAGC GTCTGTGTAT TGCGCGTGGT ATCGCCATTC GCCCGGAAGT GCTGCTGCTC GACGAACCGT GTTCGGCGCT CGACCCTATC TCTACCGGGC GTATTGAAGA GCTGATCACC GAACTGAAGC AGGATTACAC CGTGGTGATC GTCACCCACA ACATGCAGCA GGCTGCGCGT TGTTCCGACC ACACGGCGTT TATGTACCTG GGCGAATTGA TTGAGTTCAG CAACACGGAC GATCTGTTCA CCAAGCCAGC GAAGAAACAA ACAGAAGACT ACATCACCGG TCGTTACGGT TGA TTCAGGA GTGCGTT ATG GACAGTCTCA ATCTTAATAA ACATATTTCC GGCCAGTTCA ACGCCGAACT GGAAAGTATC CGCACGCAGG TGATGACCAT GGGCGGCATG GTGGAGCAGC AGCTTTCTGA TGCAATCACC GCGATGCATA ACCAGGACAG CGATCTGGCG AAGCGCGTCA TCGAAGGCGA CAAGAACGTC AACATGATGG AAGTGGCGAT CGATGAAGCC TGCGTGCGCA TTATCGCCAA ACGTCAGCCG ACGGCGAGCG ACCTGCGACT GGTTATGGTG ATCAGTAAAA CCATTGCCGA GCTGGAGCGT ATTGGCGACG TGGCGGACAA AATCTGCCGT ACTGCGCTGG AGAAATTCTC CCAGCAGCAT CAGCCGTTGC TGGTAAGTCT GGAGTCGCTG GGCCGTCATA CCATCCAGAT GCTGCACGAC GTGCTGGACG CGTTCGCGCG GATGGACATT GACGAAGCGG TACGTATTTA TCGTGAAGAT AAAAAAGTCG ATCAGGAATA CGAAGGTATT GTTCGTCAAC TGATGACCTA CATGATGGAA GATTCGCGTA CCATTCCGAG CGTACTTACT GCGCTGTTCT GCGCGCGTTC TATCGAACGT ATTGGCGACC GCTGCCAGAA TATTTGTGAG TTTATCTTCT ACTACGTGAA GGGGCAGGAT TTCCGTCACG TCGGTGGCGA TGAGCTGGAT AAACTGCTGG C GGGGAAAGA TAGCGACAAA TAA


 * A overlap between pstA and pstC

pstS - pst Operon Forward Primer
>EG10734 pstS (complement(3909548..3908508)) E. coli atgAAAGTTA TGCGTACCAC C GTCGCAACT GTTGTCGCCG CGACCTTATC GATGAGTGCT TTCTCTGTGT TTGCAGAAGC AAGCCTGACA GGTGCAGGTG CAACCTTCCC TGCGCCGGTG TATGCCAAAT GGGCTGACAC TTACCAGAAA GAAACCGGTA ATAAAGTTAA CTACCAGGGT ATCGGTTCTT CCGGTGGCGT AAAACAGATT ATCGCTAATA CCGTTGATTT TGGTGCCTCT GACGCGCCGC TGTCTGACGA AAAACTGGCT CAGGAAGGTC TGTTCCAGTT CCCGACCGTG ATTGGCGGCG TGGTGCTGGC GGTTAACATT CCAGGGCTGA AGTCTGGCGA ACTGGTGCTG GATGGTAAAA CCCTCGGCGA CATCTACCTG GGCAAAATCA AGAAGTGGGA TGATGAAGCC ATCGCCAAAC TGAATCCGGG TCTGAAACTG CCTTCACAAA ACATTGCTGT AGTACGCCGC GCAGATGGCT CCGGGACTTC CTTCGTCTTC ACCAGCTACC TGGCGAAAGT GAACGAAGAG TGGAAAAACA ACGTTGGTAC TGGCTCTACC GTAAAATGGC CGATCGGTCT GGGCGGTAAA GGTAACGACG GTATCGCCGC GTTCGTTCAG CGTCTGCCGG GTGCAATTGG TTATGTTGAA TATGCTTACG CGAAGCAGAA CAACCTGGCG TACACCAAAC TGATCTCCGC TGATGGTAAA CCGGTTAGTC CGACCGAAGA AAACTTCGCT AATGCAGCAA AAGGTGCAGA CTGGAGCAAA ACCTTCGCTC AGGATCTGAC CAACCAGAAA GGCGAAGATG CATGGCCTAT TACCTCTACC ACGTTCATTC TGATCCACAA AGATCAGAAG AAACCAGAAC AAGGCACAGA AGTGCTGAAA TTCTTCGACT GGGCGTACAA AACCGGGGCT AAACAGGCGA ACGACCTGGA TTACGCCAGC CTGCCGGATA GTGTAGTTGA ACAGGTTCGC GCTGCGTGGA AGACCAATAT TAAAGACAGT AGCGGTAAGC CGCTGTACTA A


 * Annealing - len:21, Tm:54, GC%:43
 * GC GAATTC GCGGCCGCT TCTAG atgAAAGTTA TGCGTACCAC C
 * Total length - 43 (2 + 20 + 21)

phoU - pst Operon Reverse Primer
>EG10735 phoU (complement(3905601..3904876)) E. coli atgGACAGTC TCAATCTTAA TAAACATATT TCCGGCCAGT TCAACGCCGA ACTGGAAAGT ATCCGCACGC AGGTGATGAC CATGGGCGGC ATGGTGGAGC AGCAGCTTTC TGATGCAATC ACCGCGATGC ATAACCAGGA CAGCGATCTG GCGAAGCGCG TCATCGAAGG CGACAAGAAC GTCAACATGA TGGAAGTGGC GATCGATGAA GCCTGCGTGC GCATTATCGC CAAACGTCAG CCGACGGCGA GCGACCTGCG ACTGGTTATG GTGATCAGTA AAACCATTGC CGAGCTGGAG CGTATTGGCG ACGTGGCGGA CAAAATCTGC CGTACTGCGC TGGAGAAATT CTCCCAGCAG CATCAGCCGT TGCTGGTAAG TCTGGAGTCG CTGGGCCGTC ATACCATCCA GATGCTGCAC GACGTGCTGG ACGCGTTCGC GCGGATGGAC ATTGACGAAG CGGTACGTAT TTATCGTGAA GATAAAAAAG TCGATCAGGA ATACGAAGGT ATTGTTCGTC AACTGATGAC CTACATGATG GAAGATTCGC GTACCATTCC GAGCGTACTT ACTGCGCTGT TCTGCGCGCG TTCTATCGAA CGTATTGGCG ACCGCTGCCA GAATATTTGT GAGTTTATCT TCTACTACGT GAAGGGGCAG GATTTCCGTC ACGTCGGTGG CGATGAGCTG GATAAACTGC TGGC GGGGAA AGATAGCGAC AAATAA


 * Annealing - len:22, Tm:54, GC%:41
 * GC CTGCAG CGGCCGCT ACTAGT A GGGGAA AGATAGCGAC AAATAA
 * Total length - 44 (2 + 20 + 22)