Team:Imperial College/Summary

Project Summary
{{Imperial/Box1|Modelling| The Dry Lab used computational simulations to explore the different properties of the Biofabricator. Our activities are summarized on this page. To find out more please visit the Dry Lab Hub.

Growth Curve
We have developed a simple model for the growth of B. subtilis where the rate of growth is related to the amount of nutrients available. To this purpose we have exploited the ideas put forward by last year's Imperial College iGEM team for their modelling of F2620 in a cell-free system.

Genetic Circuit
We have also built mathematical models for the time evolution of the basic genetic circuits that comprise our device. We have verified which model best describe the behaviour of the circuit better by using laboratory data.

Motility Analysis
Finally, we have carried out a detailed analysis of the swimming motility of B. subtilis, which led us, among other things, to develop a simple mechanical model for the swimming motility of B. subtilis. Using manual tracking, we were able to extract x,y coordinate data from the cell trajectory. This has allowed us to fit experimental data with our model. The data suggest that flagellar force of B. subtilis is Exponentially distributed.

All model simulations and motility data analysis were carried out with MATLAB. Cell tracking was done with ImageJ via the Manual Tracking Plugin. All our MATLAB files can be found in the Appendices section.


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{{Imperial/Box1|Testing| The testing and validation of our project can be split into three main areas;
 * Work with B. subtilis - Including characterisation of growth curves and transformation,
 * Extensive Characterisation  of new B.subtilis biobricks, Chloramphenicol resistance gene and motility
 * Production of Biomaterials in B. subtilis

If you'd like to see more information on the key results from the testing and validation, you can find it on the Results Page.

Results

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