Team:Paris/September 12

=Checking our ligases (following)=

Analysis of the ligation products by electrophosesis
1% agarose gel



Analysis of the ligation of the plasmid digested by EcoRI No heat inactivation of the ligase

Analysis of the ligation of the plasmid digested by BspHI Heat inactivation of the ligase (20 min at 65°C)