Waterloo/9 July 2008

08/07/09 Wed

[x] Diagnostic digest and gel of TTr (2 and 5, uncut and single cut) + Promoters (single cut + uncut + control) [x] full digest of rfp' and tt and gel and gel extraction [x] full digest of cI and rbs and gel and gel extraction

[x] gel of diagnostic and full digestion only half of diagnostic digest run on gel - diagnostic - Plac1 (1-5), Plac A(1-5), PlacIq(1-2), PlacOCI (1-2), Plac (control), RBS and uncuts run. - full - RBS, RBS, CI, CI, RFP', TT, TT, TT - rest of the digest had loading dye added and left in p3

[x] cut out gel for extraction tomorrow (tt, cI, rfp', rbs)

[] make competent cells (cont...) - will continue tomorrow [x] update Parts Inventory list - confirm all parts + store in same box