Team:ESBS-Strasbourg/17 July 2008

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DryLab
michi:
 * found paper PMID 18385157 which mutates TATA box and quantifies transcription :-) (King of the day)

WetLab
- dh5a (Mariel) + pxj - KCM : 10^5 cfu/µg - dh5a (Mariel) + pxj - HS : 4*10^4 cfu/µg - dh5a         + pxj - HS  : 1.5*10^5 cfu/µg - Top10        + pxj - HS  : 3.2*10^5 cfu/µg - db3.1        + pxj - HS  : 3*10^4 cfu/µg
 * Results Transformation
 * no clones for any of the three BioBricks
 * clones for the LexA-BD plasmid --> useable for Miniprep
 * counting:


 * The new BioBricks arrived so we made an overnight culture of, in LB(Amp):
 * p1010 in pSB1A3
 * BBa_c0012 (lacI)
 * BBa_c0051 (lamda cI)
 * BBa_j63001 (EYFP)
 * We plated p1010 in pSB3K3 on LB Kan


 * Digestion of the plasmid of the BBa_J63002(?) clone with XbaI and SpeI
 * 1x double digestion
 * 1x step by step digestion
 * result:
 * digestion seem to have work out in both cases
 * but the gel shows a high contamination with low molecular RNA, so we know now that RNase is crutial in the eluation buffer
 * the identity of the clone is still not verifified, because the size small fragement could be determinated
 * but as the new BioBricks arrived today, there is no longer an interest to go on working on this clone

General

 * Modeling meeting with Mr. Haich

Quote of the day
Marius loves Michis Kidneys!