Team:Warsaw/Calendar-Main/2 September 2008

Purification of proteins: A-alpha Piotr, Emilia pET15b+A_alpha in Rosetta strain from overnight culture inoculated in fresh LB and cultured until OD=0.5. Culture was induced with different concentrations of IPTG at 22&deg;C and 37&deg;C. Samples were collected twice: after 3h and next day (samples were centrifuged and frozen).