User:University of Washington/7 July 2008

BioBrick Promoter Measurements
- Thawed glyerol stocks for TOP10 cells containing promoter constructs I20260, I20268, I20269, and I20270.

- Inoculated cultures on four different agar plates containing LB and kanamycin to prepare for measurements.

LuxR from AraC and TetR
- Selected five colonies from old AraC plate to grow overnight: 3 ml Tsy + 3 ul Amp.

- Plated AraC from glycerol stock in Agar LB + Amp plate.

- Resuspended four primers in deionized water to the concentration 100 pmole/ul, later stored in -20 Celsius.

LuxR from pLac
- Started overnight culture of I736004 for sequencing and glycerol stock.

- Started overnight culture of R0010 for glycerol stock.

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