Alberta NINT/25 June 2008

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lab work (SD): Isolated DNA (K102008) from 24/06/08 overnights using QIAprep spin miniprep protocol. Set up K102008 for sequencing. Digested K102002 and K102008 with EcoRI + PstI, ran on 2% agarose gel at 110V for 50 min. Excised large fragments from the gel. Set up 8 overnights from the 14 colonies found on the K102008 plate from 24/06/08.

lab work (JD): Decided to try AGAIN to recreate K102007 but using older versions of K102005/B+N that seemed to be             cut better when looking at gels and ligating them to the newly annealed TA0/B+N (a combination I              still haven't tried) Created K102007(1): K102005/B+N from June 18, and TA0/B+N newly annealed Created K102007(2): K102005/B+N from June 12 and TA0/B+N newly annealed Created K102006: K102003/B+N and TA0/B+N newly annealed Transformed XL1-B cells with the ligations and negative controls Plated cells