Team:NTU-Singapore/Notebook/25 September 2008



  

=Thursday 25 September= {|border="1" style="background-color:#ffffcc;" cellpadding="20"
 * Minipreps for LsrA-YFP, followed by digestional gel check.
 * Result: correct bands: 1kb insert (LsrA-RBS with YFP generator); 2kb vector
 * Make LuxS mutants more competent. Then transform detection system and production system into these newly made LuxS mutants.
 * Grow W3110 in LB plus 0.8% glucose for 4 hours, centrifuge and take the supernatant (which contains AI-2), store at -20 degrees C.
 * Grow W3110 in LB plus 0.8% glucose for 4 hours, centrifuge and take the supernatant (which contains AI-2), store at -20 degrees C.