Imperial College/2 October 2008

=2 October 2008=

Wetlab

 * The ligations of various PCR products into AK3 were transformed and single colony PCR was carried out to check for correct ligation. The following PCR products were ligated:
 * EpsE 5' integration site (5'E, ~500bp)
 * EpsE 3' integration site (3'E, ~500bp)
 * Spectinomycin resistance gene (spec,800bp)
 * The pSB primers were used on the following cycle:
 * 1 cycle - 95oC for 30 sec
 * 30 cycle - 95oC for 30 sec, 60oC for 45 sec, 72oC for 1 min
 * The following colonies appear to have correct inserts: 5'E1, 3'E2, Spec1