Imperial College/5 August 2008

= 5 August 2008 =

Briefing

 * Yanis Journal Club today (1400) - Environmental Sensing, Chris Wright
 * Drylab will be continuing with tutorials
 * Wetlab will order sequences and reagents today
 * Testing bacterial synthesis... How do we know our material is being secreted rather than just the cells being lysed on production?
 * Produce without signal sequence
 * Tack on a reporter that is not secreted - if that turns up on the gel with the biomaterial we've got lysis
 * Protocols: James, Chris, Tom & Krupa will split into two groups - Cloning and B. subtilis culturing
 * Each will organise the relevant protocols, but we'll all pitch in for the actual wetlab work
 * QQ will be away for 2 weeks, possibly longer
 * Email for microscope training to be sent today
 * Microscope work will require a motile E. coli strain
 * Journal Clubs coming up:
 * Thursday: Erika & Clinton - T7 Promoters
 * Friday: Krupa & James - Craig Venter
 * Monday: Chris and Prudence - Quorum Sensing
 * Tuesday: Tom and Yanis - Division and death in bacteria

Debrief
Vincent put up a list of things he wants to know about... sometime... It's a good way to structure our work, anyhow. It's pasted below, please put your names next to things you'll take care of! Basically I've made a few pages (linked in the Navbar) for each team, and we need to put up concise explanations and summaries of progress. Eventually we'll probably copy and paste a lot of this to the official wiki; so when you're doing internal links, please call them Team:Imperial_College/PageName. That way when we copypasta everything over, pages will already point to the right place... Oh, and if you make a new page, remember to put at the top and  at the bottom. [:

"Hi iGEMers,

I hope you are all doing well. I would be really interested to catch-up with the iGEM project.

Before we meet again, could someone send me a list of links from your OWW wiki related to the following topics (please no .doc or .pdf as they are not collaboration friendly):

1- Design of your final construct + basic description of its expected behaviour (no sequences needed) - Tom

2 - Design of your test constructs + basic description of their use (no sequences needed)- James

3 - Protocols:
 * B.subtilis culture
 * B.subtilis competent cell preparation
 * B.subtilis DNA chromosomal integration
 * B.subtilis and time lapse microscopy
 * B.subtilis motility assay
 * test construct protocols
 * others

4 - Your wet lab shopping list - James

5 - Update on the motility analysis - Erika

6 - Update on the biomaterial production - Prudence

7 - Update on the light sensing system - Clinton

8 - description of the different working groups (people involved and scope of the working group) -

9 - Your current planning for the project and sub-projects (deadlines and deliverables)

10 - Also it would be great if you could also comment on the current challenges you are facing in the project - everyone
 * thanks and see you soon, Vincent."