User:University of Washington/14 July 2008

LuxR from AraC and TetR
- Got some single colonies from the old plates(from Friday). New plates had too many cells in them, no good single colonies.

- A colony were selected from each section of the two plates (12 total) to prepare the overnight cultures.

LuxR from pLac
-Overnights of parts R0010, I0462, and E0420 were started for assembly of parts.

BioBrick Promoter Measurements
- Glycerol stocks of the TOP10 transformed with I20260, I20268, I20269, I20270, and the empty control cells were plated on four kanamycin plates, and one plate without antibiotic, respectively.

- Filter paper for the following parts were removed from the Registry binder, soaked in TE buffer, then centrifuged at 15,000 rpm for 3 minutes: I20259, I20261, I20270, E0240, P1010, J23100, J23100, J23103, J23104, J23106, J23107, J23108, J23109, J23110, J23111, J23112, J23114, J23115, J23117, J23118, J23119.

Lambda Red Recombineering of RP4 (Bryan)
Continued troubleshooting of last week's PCR. Attempted to amplify TetR vector targeted to RP4. Rather than using plasmid template as on Friday, used TetR template provided by graduate advisor, which had been validated in her experiments. Ran parallel reactions with primers designed both by myself and Scott. PCR failed.

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