Imperial College/1 October 2008

=1 October 2008=

PCR

 * Today we carried out a Single colony PCR to check a the ligation of parts into biobrick vectors. The image below summaries the results of the PCR. The labels correspond to the following:
 * 6L - LipA-Elastin into AK3 vector
 * X- XylR into AK3 vector
 * Pos-Positive control of AmyE 5' (577bp)
 * Neg- Negative control, no DNA template.




 * The following reaction conditions were used with the Pfu ultra enzyme:
 * 1 cycle - 95oC for 30 secs,
 * 30 cycles - 95oC for 30 sec, 60oC for 45 seconds,72oC for 1 min
 * The results show that 6L has failed whilst the XylR has sucessfully been ligated.