Team:Chiba/parts/BBa K084007



parts usage
BBa_K084007(plac+rbs+LasI(no LVA))



日本語:IPTGにより、シュードモナス由来の3OC12HSL合成酵素,LasIを発現するデバイス.

English:

Experiment
To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.
 * 1) Transformed Senders into E.coli strains(XL10GOLD or JW1908) and Receiver into E.coli strain(JW1908).
 * 2) Inoculated them independently in liquid media. Incubated at 37°C 12h
 * 3) Mixed them.
 * 4) Incubated at 30°C.
 * 5) Measured intensity of green fluorescence at regular time intervals.
 * crosstalk experients at various conditions.
 * 1) E.Coli strain JW1908 or XL10Gold.
 * 2) Reaction Temparature 37 or 30.
 * 3) Cell number.

Senders:XL10Gold, Receiver:JW1908, 37°C






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