Team:Hawaii/Notebook/2008-08-24

= Things we did today =

Verification of transformants

 * Grace

Verification of plasmid preps (cont.)

 * Grace


 * PCR machine crashed. Will redo PCR tomorrow.

Restriction Digest

 * Margaret


 * pSB1A3 with EcoRI and PstI, pSB1A3 with SpeI & PstI, rep (gel purified PCR product from 8/11/ with XbaI & PstI, and P1 lytic region (gel purified PCR product from 8/11/08) with XbaI & PstI.

Ligation & Transformation into DH5 alpha cells

 * Margaret


 * I14032 (synthetic construct of lac promoter) + B0030 (synthetic construct of 0.6 RBS), I14032 (synthetic construct of lac promoter) + B0034 (synthetic construct of 0.1 RBS)
 * pSB1A3 + [I14032+B0030], pSB1A3 + [I14032+B0034], (-) control= pSB1A3 to itself, (+) control for transformation is pUC18.

Colony PCR Gel from yesterday

 * Margaret



= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"