Team:KULeuven/10 July 2008

Practical
9-17h in labs and room 00.210 of the LBI Present: all students (Nick and Stefanie were excused in the afternoon)

Results

 * (Hopefully) Final workout of the project. See the figure below.
 * Smaller problems, we got rid of the miRNA again and decided to go for a complete antisense LuxI. This will be part of a new memory system that will allow HSL to accumulate if and only if an input signal has been received once in the past.
 * We also inserted the TetR together with LuxR under control of a constitutive promotor.
 * Most of the the Ribosome Binding Sites have been changed to less efficient ones in order to allow modification to weaker AND stronger ones if necessary.
 * Pulse generator has also been redone, based upon the first system with a key and a T7 RNA polymerase. This time however, the key comes first. We hope that its half life is sufficiently short.
 * Modelling in CellDesigner: inverter (modified) + cell death (new), input-ouput (new), memory (new), filter (modified) + cell death (new), pulse generator (new).
 * A presentation has been prepared for next week to inform the advisors, instructors, students and other fanatics.