Team:University of Ottawa/1 August 2008

  Untitled Document  

 Home  Welcome Announcements</li> </ul> The Team</a> <ul> Who We Are</a> <ul> Advisors</a></li> Undergrads</a></li> </ul> </li> What We've Done</a></li> Where We're From</a></li> Contact Us</a></li> </ul> </li> The Project</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Project_Overview">Overview</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Pulsate_Gene_Expression">Expression</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Cell-to-Cell_Communication">Communication</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Oscillatory_Dynamics">Oscillation</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Applications">Application</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Journal_Club">Journal Club</a></li>

</ul> </li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Parts">BioBricks</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Modeling">Modeling</a></li> <li><a class="MenuBarItemSubmenu" href="http://2008.igem.org/Team:University_of_Ottawa/Wet_Lab">Wet Lab</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Lab_Protocols">Lab Protocols</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/WetWare">WetWare</a></li> </ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Notebook">Notebook</a></li> <li><a class="MenuBarItemSubmenu" href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors">Sponsors</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Acedemic_Sponsors">Academic</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Research_Sponsors">Research</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Corporate_Sponsors">Corporate</a></li> </ul> </li>

</ul> <script type="text/javascript">

Chris
Digestion Experiment


 * <li> All samples inactivated at 65&deg;C for 20 minutes.
 * <li> Ran the products on a 1% gel at 80V for 40 minutes.
 * <li> No visible difference between: 5+ and 5-; and 15+ and 15-. 10- matches 1 hr better than 10+, which showed incomplete digestion. All samples other than 1 hr showed varying degrees of incomplete digestion.
 * <li> Microwave protocol is therefore not useful for time frames tested. However, current digestion processes can be shortened from 1 hour to 15 minutes.

Digestion of Constructs OA, OB and T Amp Product


 * <li> Deactivated at 80&deg;C for 20 minutes
 * <li> Added 1&mu;L ligase and 1&mu;L ATP to tubes.
 * <li> Left one sample over the weekend to ligate, another for 5 seconds in the microwave, then to the 4&deg;C fridge, and one for 10 seconds in the microwave then to the 4&deg;C fridge.
 * <li> Inoculated 5 samples each of Matt's 3:1 and 6:1 PTP2/PSSA42 construct for removal tomorrow by Corie.

Dan
Transformation
 * <li> p1T(0A0B) and pSSA14 + 0B were transformed

Gel
 * <li> Gel was run of p1T right after the first gel extraction and after digestion with ClaI and ligation