Team:UNIPV-Pavia/Notebook/Week14

Week 11: 08/21/08 - 08/22/08
08/21/08
 * Sequencing results:
 * J23100-B0030-C0012-B1006-R0010 - completely wrong sequence
 * C0062 - OK
 * Lig.12 - OK
 * Lig.22 - point mutation C->T
 * Lig.30 - point mutation C->T
 * We decided to restart from Lig.12, whose sequence was correct, to perform ligations Lig.22, Lig.30, Lig.34 and Lig.35.


 * We prepared 0.5 l of LB + Amp for plates.


 * We infected 9 ml LB + Amp with 30 µl of B0030-C0061-B1006-R0062-B0030-E0040-B1006 and Lig.12 glycerol stocks.

08/22/08
 * Glycerol stocks/miniprep for B0030-C0061-B1006-R0062-B0030-E0040-B1006 and Lig.12.


 * We planned experiments for qualitative and quantitative tests.