Team:University of Lethbridge/Notebook/Project2September

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Roxanne
-Picked the colonies which appear to have amplified during the screening PCR, and Incubated them in LB+amp media at 37.0C overnight.

Roxanne
-Plasmid Prepped the colonies which were incubated overnight. Ran Plasmids on a 1% Agarose Gel at 100V for 27 minutes.

Nathan Puhl
-Visulaized the Gel, and chose appropriate cells to glycerol stock.

Roxanne, Nathan Puhl
-Performed a Restriction Digest on RS1 and RS2 from pGEM T-easy using XbaI and SpeI. Ran overnight at 37.0C

Nathan
-Reran the PCR of the riboswtich from the primers and has been working on the Riboswitch throughout the month.

Gel of RS_cheZ and RS1, RS2



Gel of the New Riboswitch PCR product