Team:Chiba/jk/β/week 4

>送受信班

7 September, 2008
-->(6/9) Digestion Test
 * Sample DNA 1 : BBa_R0010(unknown)
 * Sample DNA 2 : BBa_R0010(100ng/μL)


 * --->Gel Check


 * Left side:BBa_R0010 Sample No.1
 * Right side:BBa_R0010 Sample No.2
 * Left side:BBa_R0010 Sample No.1
 * Right side:BBa_R0010 Sample No.2

-->(8/9)

8 September, 2008
-->(7/9)

Gel Extract


 * --->Zymo Clean
 * ->15μL?

9 September, 2008
-->(6/9)PCR of I9030

--->Gel Check

--->Gel Extract

--->Zymo Clean

10 September, 2008
-->(10/9)
 * Colony Count


 * 1) LuxI
 * 2) LuxI with LVA
 * Las
 * 1) Background(R0010)
 * 2) Background(C0261)

Pick colony

Colony-PCR
 * Colony PCR of 10 colonies from ligation plate(9/9-(2)) and 3 colonies from ligation plate(9/9-(3)) one from control plate(BBa_F2620(2007)).



??
 * 95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃

??

-->Gel Check

--->(28/8)Miniprep

elute 50μL

13 September, 2008

 * Gel Check
 * BBa_C0161(2007)
 * BBa_J04500(2007)
 * BBa_R0010(2007)

-->Digestion
 * BBa_C0161(2007)
 * BBa_J04500(2007)
 * BBa_R0010(2007)