Team:Warsaw/Calendar-Main/2 June 2008

Rifampicin test #3 Piotr Conclusion: AID seems to work, induction of AID may reduces the growth rate

Preparation of pMPMT5-AID+AIDT7 construct Michał K. PCR for AID transcriptional fusion with translational fusion AID+T7 RNA-polymerase.

Primers:

AIDlNrH and T7pXbSal

Template DNA: translational fusion AID+T7 RNA-polymerase (pMPMT5+AID-T7)

Elongation temperature: 55°C

Time: 4 minutes

Number of cycles: 20 and 25

Blue/white and rifampicin test #1 Michał L., Ewa, Marcin  Transformation of E. coli TOP10 with pZC320</a>.</li>

<li> Plating transformants on LB + ampicillin (30 μg/mL) + X-gal + IPTG.</li></ol>