Team:Warsaw/Calendar-Main/21 October 2008

Preparation of OmpA-linker-omega-linker (BBa_K103016) Piotr

Inoculation of few more colonies from plate with ligation of pACYC177 + OmpA-linker-omega-linker (BBa_K103016) (from 16 October) to LB with kanamycin.

Preparation of alpha_linker under PT7 (BBa_K103019) Michał K.  Isolation of plasmids from cultures inoculated on previous day (pSB2K3 + alpha_linker under PT7 (BBa_K103019)</a>).</li>

<li>Control digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found. Fig. 1</a>.</li></ol>

<img src="http://2008.igem.org/wiki/images/1/11/Traw2_19_10_2008.jpg" width=200></a> Fig. 1. EcoRI/PSTI digest of pT7_alpha_link

1-3. EcoRI/PstI digests of pT7_alpha_link 4. Marker

Preparation of AID under pBAD/araC (BBa_K103002)</a> Michał K. <ol><li> Inoculation of few more colonies from plate with ligation of pSB1A3</a> + AID under pBAD/araC (BBa_K103002)</a> to LB with ampicillin. </li> <li>Isolation</a> of plasmids from cultures inoculated on previous day - pSB1A3</a> +AID under pBAD/araC (BBa_K103002)</a>.</li>

<li>Control digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - still no proper clones found. Fig. 2</a>.</li> </ol>

<img src="http://2008.igem.org/wiki/images/a/ac/Traw4_16_10_2008.jpg"></a> Fig. 2. EcoRI/PstI digests of BBa_K103002 1. Marker 2-6. EcoRI/PstI digests of BBa_K103002

Preparation of OmpA_linker_alpha_linker (BBa_K103014)</a> Michał K. Inoculation of colonies from plate with ligation (pSB2K3</a> + OmpA_linker_alpha_linker (BBa_K103014)</a>) to LB with kanamycin.