Team:ESBS-Strasbourg/21 July 2008

< back

WetLab

 * Miniprep of TetR (MM)
 * Overnight culture of p1010 A2 (LB+Amp) and p1010 K3 (LB+Kan), i.e. 200µl of little overnight culture in 100 ml medium
 * Overnight culture of Top10 cells from Invitrogen (cultivated on a plate) to make them competent tomorrow
 * PCR of VP16 (3 different templates) Tup1,LacI,CI,TetR @ 48,50,52,53 °C -> none worked