Team:University of Ottawa/6 August 2008

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 Home  Welcome Announcements</li> </ul> The Team</a> <ul> Who We Are</a> <ul> Advisors</a></li> Undergrads</a></li> </ul> </li> What We've Done</a></li> Where We're From</a></li> Contact Us</a></li> </ul> </li> The Project</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Project_Overview">Overview</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Pulsate_Gene_Expression">Expression</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Cell-to-Cell_Communication">Communication</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Oscillatory_Dynamics">Oscillation</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Applications">Application</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Journal_Club">Journal Club</a></li>

</ul> </li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Parts">BioBricks</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Modeling">Modeling</a></li> <li><a class="MenuBarItemSubmenu" href="http://2008.igem.org/Team:University_of_Ottawa/Wet_Lab">Wet Lab</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Lab_Protocols">Lab Protocols</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/WetWare">WetWare</a></li> </ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Notebook">Notebook</a></li> <li><a class="MenuBarItemSubmenu" href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors">Sponsors</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Acedemic_Sponsors">Academic</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Research_Sponsors">Research</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Corporate_Sponsors">Corporate</a></li> </ul> </li>

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Chris
Minipreparation and Confirmation of PTP2/PSSA42 samples
 * <li> Control (tip only in LB+Amp) was clear
 * <li> Followed miniprep protocol included in kit
 * <li> Spilled one tube; concentration expected to be zero. Also, did not mix neutralisation solution as outlined in the protocol.
 * <li> Measure absorbance of isolated DNA: all samples very low and poor purity.
 * <li> Digested aliquots of all samples with PstI despite low concentrations. Expected bands (1246, 699, 5685) appeared on a 1% gel; however were faint and inconclusive.
 * <li> While the gel was running, performed another miniprep of the same ten samples, following protocol exactly in order to increase DNA concentrations.

Continuation of Ligation Optimisation
 * <li> Ran all products on a 1% gel for 40 minutes at 80V.
 * <li> All ligations appeared exactly the same on the gel; future ligation protocol will be shortened from overnight to a minimum of six minutes.