Virginia/8 July 2008

Goals

 * Prepare overnight broth of single successful colony of Promoter + RBS transformation
 * Plate Screening plasmid (psb1a10) when it arrives from iGem
 * Try again with ligation of RFP and GFP enzyme cut DNA
 * Transform this ligation and pray that it grows in the incubator
 * Run a gel of linearized and cut DNA of BP1 and terminator assembly to verify transformation
 * Begin ligation of standard RBS with BP1 gene to begin parallel assembly of our final operon

Accomplished

 * Starting from maixprepped DNA we cut:
 * E0240, I715039, B0034 (dubious, see above), BP1
 * Short Ligations: (45min)
 * Test Vector 1: I715039 + E0240
 * Did a ligation each for non-purified DNA (after digestion) and purified DNA.
 * B0034 (RBS) + BP1
 * Only non-purified post-digestion DNA
 * Plated all short ligations
 * Long Ligations: (overnight @ room temp)
 * Test Vector 1: I715039 + E0240 (unpurified)
 * B0034 (RBS) + BP1
 * Plated screening plasmid pSB1A10 that we received today

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