Team:Warsaw/Calendar-Main/2 July 2008

Change of the reporter from pZC320 with B-galactosidase to GFP or RFP Piotr, Weronika, Emilia 

 Gel electrophoresis and gel-out of proper bands. 

 Ligation of pZC320 with standard parts: BBa_E0840(GFP genetrator) and BBa_J04450 (RFP generator). 

Chemotransformation of E.coli TOP10 with ligation products. Plating transformants on LB+Amp30+X-gal+IPTG.</li></ol>

Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega Paweł <ol>

 Clean-up</a> of overnight digest.</li>  <A HREF="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</A> of OmpA_alpha (from Michał K.) and pET15b</a>. </li>  <A HREF="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</A> of OmpA_omega (from Michał K.) and pET15b</a>.</li>  Transformation</a> of E. coli TOP10</a> strain with ligation products. </li>  Transformants plating on LB + ampicillin.</li></ol>