User:University of Washington/25 June 2008

Yeast Shuttle Plasmid
- An agar plate containing yeast strain BY4741 (MATalpha, ΔBar1::Kan MX, his3Δ1; leu2Δ0; met15Δ0; ura3Δ0) was obtained.

- The yeast was incubated overnight at 30 degrees Celsius.

LuxR under AraC and TetR
- Obtained BBa_R0040 (TetR), BBa_R0080 (AraC)

- transformed and plated with Amp

LuxR under pLac
- Obtained parts R0010, I0462, and J04430.

- R0010 and I0462 were transformed into MG strains, and plated on Amp.

- J04430 was transformed into DH strain, and plated on Amp.

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