Team:Warsaw/Calendar-Main/20 August 2008

Tests for ampicillin resistance given by protein added to medium Piotr

Inoculation of all pACYC177-OmpA variants of constructs to LB + 0.25 mM IPTG, kanamycin, ampicillin (50 μg/ml) and 100 μg/ml of protein (Z-alpha and Z-omega; negative control without protein). Measurement of growth after 5 hours and on next day.

Cloning of protein A DNA to GeneArt plasmid Antoni Transformation of E. coli TOP10 strain with ligation products from previous day.

Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omega Michał K.  Colonies from both plates(pACYC177+OmpA_alpha_A_omega and <a href=http://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BOmpA-alpha-Z-omega>pACYC177+OmpA_alpha_Z_omega</a>) was inoculated to liquid LB with kanamycin (only two colonies have grown on each plate).