Edinburgh/1 September 2008

< Previous Entry | Notebook Index | Next Entry >

Monday 1 September 08

 * Minipreps M182 to M193:
 * M182 to M187: pSB1A2+cenA.
 * M188 to M193: crtBI+appY.
 * Followed by double digets using EcoRI and PstI (YAN)
 * Fusion PCR:
 * P91: P88 (SU1) and SOB2 ligation products using Forward primer zm1 and Reverse primer sob r1.
 * P92: P88 (SU1) and SOB2 ligation products using Forward primer zm1 and Reverse primer sob.rbs.
 * P93: P89 (ISO2) and SOB2 ligation products using Forward primer zm2 and Reverse primer sob r1.
 * P94: P89 (ISO2) and SOB2 ligation products using Forward primer zm2 and Reverse primer sob.rbs. (YAN)
 * Overnight cultures 1 to 4 of appY+dxs+lims from plate 155 (YAN)