Newcastle University Wetlab/27 August 2008

Wednesday 27th August

 * ON cultures were analysed:

- pGFP-rrnB grown on ampicillin - cells had lysed (medium was clear with large bubbles at the surface).

- pJWV021 grown on ampicillin - frozen glycerol stock made (see protocol).

- pUC57-ncl08 grown on ampicillin - frozen glycerol stock made.


 * A colony stab was taken of pGFP-rrnB using 10μl spectinomycin in 10mL LB as the culture medium.