Team:Mexico-UNAM-IPN/Horizontal-Transfer/26 September 2008

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COMPETENT CELL PREPARATION

DH5α JM101 JM109

JM101 and JM109 strains.
 * 1) Individial collonies were taken from overnight cultures from the stocks of the DH5α
 * 1) 5ml of sterile LB + collony. Incubation at 37°C, 150rpm.
 * 2) The strains JM109 and JM1101 reached the apropiate density (exponential growth phase) after 2hours of incuabation.
 * 3) Incuabation 10 minutes at 4°C.
 * Red:JM101
 * Blue
 * Green

NOTE: The experiment were suspended due the fact that cultures wer frozen.