Team:Chiba/Calendar-Home/18 August 2008

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17 August 2008 <|> 19 August 2008

Team:Input
Transformation competent cells : XL10Gold(Cm),LB-agar(KanR)
 * BBa_S01416(2006)
 * BBa_S01416(2007)

Team:communication

 * PCR
 * BBa_C0070(2008)
 * BBa_C0076(2008)
 * BBa_C0077(2008)
 * BBa_C0078(2008)
 * BBa_C0079(2008)
 * BBa_F2620(2007)･･･control


 * 95°C,5min -> ( 95°C,1min -> 52°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C


 * --->Gel Check


 * Transformation

competent cells : XL10G


 * BBa_C0070(2007)
 * BBa_C0070(2006)
 * BBa_C0076(2007)
 * BBa_C0076(2006) -> No colonies on plates(19/8)
 * BBa_C0078(2007)
 * BBa_C0078(2006)
 * BBa_C0170(2007)
 * BBa_C0170(2006)
 * BBa_C0178(2007)
 * BBa_C0178(2006)


 * -->(20/8)Mini prep

Team:Output
no work