Transforming into DH5α E. coli


 * Add 12.5μl plasmid or ligation mixture to 100μl cells on ice.
 * Mix by pipetting carefully up and down.
 * Incubate tubes on ice for 45 minutes.
 * Incubate in a 37°C water bath for 3 minutes.
 * Add 300μl LB to the mixture and mix by pipetting carefully up and down.
 * Incubate in a 37°C water bath for 60 minutes.
 * Plate onto antibiotic agar.