Waterloo/17 July 2008

08/07/17 Thurs [x] miniprep of transformants inoculations (rfp'')

[x] diagnostic digest of transformants and c0051, c0052 [x] gel of diagnostic digest

[x] glycerol stocks of PFU innoculations

[x] p/s/i c0052'

[x] if gel worked, full digestion of Plac, POCI, Pconst (two of each), rfp, gfp [x] gel of full digestion [x] cut out bands of full digestion

[x] Inoculate Placgfp'' into liquid culture to test that way under UV

[x] inoculate c0052-1(B50), c0052-2(b51), c0051

[] dna extraction and ligation

[x] Run PCR and gel to test Pfu in Pfu specific buffer - PCR didn't seem to work.