Team:Chiba/protocol/ligation/dephosphorylation

>Protcol

dephosphorylation

SAP: Alkaline Phosphatase (Shrimp)

DNA fragment 1~10 pmol Shrimp Alkaline Phosphatase (1~5 μ l) 1~5 U 10X SAP Buffer 5 μ l Sterilized distilled water up to 50 μ l
 * Mix


 * 1) Incubate at 37℃ for 15~30 min.
 * 2) Incubate at 65℃ for 15 min. (for inactivation by heat treatment)
 * 3) Purify the DNA with Zymo DNA Cleam&Concentrator Kit

READ:Zymo cleam