Wiki/Team:Warsaw/protocols list

 Purification of His_Z_alpha and His_Z_omega Purification of His_A_alpha

Testing various hunter/prey combinations

Plasmid DNA isolation</li> DNA isolation from agarose gel</a></li> DNA purification after enzymatic reaction</a></li> Genomic DNA isolation</a></li> Preparation of chemocompetent bacteria</a></li> Preparation of electrocompetent bacteria</a></li> Chemotransformation</a></li> Electrotransformation</a></li>

Ligation</a></li>

<li><a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#blunting">Blunting</a></li> <li><a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#concentrations">Standard concentrations of antibiotics and other supplements </a></li> <li><a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#rif">Rifampicin test </a></li> <li><a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">Removing 5' phosphate groups from DNA ends </a></li> <li><a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#taxi">TAXI protocol (Tet+Ap 30+X-Gal+IPTG) </a></li> <li><a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#bca">Protein concentration measurement (BCA method) </a></li> <li><a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">DNA digest </a></li> <li><a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR </a></li> <li><a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcl">Polymerase Chain Ligation (PCL) </a></li>

</ul>

If not stated otherwise, all agarose gels bear <a href="http://www.fermentas.com/catalog/electrophoresis/generulers.htm#Mix">Fermentas GeneRuler (100-10000 bp)</a> ladder. PAGE gels bear <a href="http://www.fermentas.com/catalog/electrophoresis/marksm0671.htm">PageRuler Fermentas Prestained protein ladder</a>.

<img src="http://2008.igem.org/wiki/images/5/52/Spacer.gif" width="1" height="530" border="0" alt="" />