Waterloo/13 August 2008

08/08/13 Wed

[x] gel of full digestion (c0052', c0051', T7 gene6-27) [x] gel extraction [x] check gel of extraction [x] ligation of parts from extraction and c0052', c0051' [x] transformation (extraction and c0052', c0051')

[] p/s/i of transformants

[x] glycerol stock of S.meliloti (x3) [x] reinoculate C0052', c0051' in Ap

[x] another PCR of PHB A and B (leave overnight)

[] send 2 of each gene for sequencing

[x] PCR of PHB genes (if primers arrive) - try using Taq first - only PHBC worked [x] check gel of PCR

[x] if Taq PCR successful, run again w/ Phusion; else run troubleshooting PCR w/ Taq