Team:Hawaii/Notebook/2008-09-19

= Things we did today =

Plasmid prep of BB-pRL1383a

 * Grace

Construction of secretion device

 * Grace


 * PCR of nir+rbs, rbs+GFP, slr1+GFPf, pilA, slr2
 * Overnight RE digest:
 * EcoRI/SpeI
 * nir+rbs
 * rbs+GFP
 * slr1+GFPf
 * nir (PCR product from before)
 * XbaI/PstI
 * rbs+GFP
 * slr1+GFPf
 * pilA
 * slr2
 * EcoRI/PstI
 * pSB1A3


 * Krystle


 * Transformation of overnight ligations

Gel to confirm transformants
Margaret

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"