Team:Mississippi State/Making Blasticidin Stock Solution


 * Blasticidin Stock Solution is used to apply to Pichia cell cultures when we use them. It will kill any cells without the appropriate vector in them.


 * 1) Pichia cultures require the addition of 100ug/ml working solution.
 * 2) Measure out desired milligrams of powdered antibiotic
 * 3) For each mg antibiotic, add 10ul water.
 * 4) Vortex until dissolved.
 * 5) If doesn't dissolve, add 10ul water more per mg antibiotic.
 * 6) Vortex until dissolved. If double amount of water is added, you now have 50ug/ml working solution.
 * 7) Freeze -20C freezer.


 * Note: If more water is added to dissolve antibiotic, the working solution is weaker, and more needs to be added to LB.
 * If you have 100ug/ml working solution, add 1ul for every 1ml LB.
 * If you have 50ug/ml working solution, add 2ul for every 1ml LB.