Team:Chiba/Calendar-Home/30 August 2008

>Home | Notebook

29 August 2008 <|> 31 August 2008

Meeting with UmeG
the ppt (in Japanese) for our weekly meeting
 * 1) [[media:080830input.ppt|team-input]]
 * 2) [[media:0830-soujushin-2.ppt‎|team-communication]]
 * 3) [[Media:Output_chiba_080830.ppt|team-output]]

Team:Input
Digestion

x-RBS-cI-s-p(780bp)

-->30μlGel extraction

-->Zymo Clean

eluted with 5μL of Nuclease free water(NFW)

Gel Check(1μl)

-->OK

(remains of the solution(4μL)-->Ligation)

Digestion

-e-x-Ptet-s-p-(PMB1,Amp)(Insert:54bp,Vector:2079bp)

-->30μlGel extraction

-->Zymo Clean

eluted with 10mL of NFW.

-->SAP

eluted with 5μL of Nuclease free water(NFW)

Gel Check(1μl)

-->OK

(remains of the solution(4μL)-->Ligation)

Ligation

-->Transformation(XL10G)

-->CFU40(b.g:22)

PCR of 16 colonies)

-->Gel Check

result:One colony was apparent.

Picked the colony and incubate in 2mL of LB.

-->Mini prepped

Digestion

x-RBS-cI-s-p(780bp)

Gel Extract

-->Zymo Clean

-->eluted with 5μl of NFW

-->Gel Check

-->Gel Check-->OK

Digestion

-e-x-Ptet-s-p-(PMB1,Amp)(Insert:54bp,Vector:2079bp)

Gel Extract

-->Zymo Clean

-->eluted with 10μl of NFW

-->SAP

-->left for an hour at 37 degrees,for 15 minutes at 65 degrees.

-->added 90μL Binding Buffer and vortexed.

-->Zymo Clean

-->eluted with 5μl of NFW

Gel Check

-->Gel Check-->OK

Ligation

-->left at rest at room temparature(25　degrees) -->Transformation(XL10G):

-->CFU40(b.g:22)

Team:Communication

 * Transformation
 * competent cells : XL10G
 * BBa_I9026(2007)
 * BBa_I9030(2006)
 * BBa_S03154(2007)
 * BBa_S03156(2007)
 * BBa_S03158(2007)
 * BBa_S03160(2007)
 * BBa_C0062(2007)
 * BBa_C0179(2007)


 * --->(31/8)Mini prep


 * (29/8)--->Mini prep
 * insert:BBa_C0170 + vector:BBa_J04500
 * insert:BBa_C0178 + vector:BBa_J04500


 * --->Digestion Test
 * insert:BBa_C0170 + vector:BBa_J04500 -> Sample No.1~4
 * insert:BBa_C0178 + vector:BBa_J04500 -> Sample No.5~8
 * BBa_T9002(2007) -> Sample No.9
 * Single Digestion of Sample No.1~9 -> Sample No.10~18
 * Double Digestion of Sample No.1~9 -> Sample No.19~27




 * --->Gel Check

Team:Output
Transformation


 * mcherry

PCR


 * BBa_J52008(1)