Edinburgh/12 July 2008


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Saturday 12 July 08

 * Result of transformation: plate 30 (pSB1A2+crtB 100μl), 22 white and 10 blue; plate 31 (pSB1A2+crtB 900μl), too numerous to count, about two thirds white; a few blue showed signs of phage-like lysis. For crtI mutant, plate 32 (100μl), 4 white and 2 blue; plate 33 (900μl), 143 white and 25 blue. White crtB colonies were patched to Plate 35, crtI to Plate 36.
 * Set up overnight cultures of possible rbs+dxs clones from plate 34 for minipreps.


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