Minnesota/27 July 2008

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 * 1. Spec the plasmid prep products: Place 10 samples into 10 separate plate wells. 11 wells total were used: 1st well was the control so it had 36uL of distilled water and 4uL of elution buffer (since want everything except DNA to be control group), then 2-11 wells had 1-10 plasmid prep products. 2-11 wells had 36uL of distilled water added and 4uL of plasmid prep products. Results:
 * a. (1) Lac I #1 = [DNA] ng/uL is 45, [DNA] ug/uL is 0.045   HIGH/GOOD
 * b. (2) Lac I = [DNA] ng/uL is 35, [DNA] ug/uL is 0.035    HIGH/GOOD
 * c. (3) Lac I = [DNA] ng/uL is 35, [DNA] ug/uL is 0.035   HIGH/GOOD
 * d. (4) BV:Lac #2 = [DNA] ng/uL is 10, [DNA] ug/uL is 0.001  LOW/POOR
 * e. (5) BV:Tet #1 = [DNA] ng/uL is 0, [DNA] ug/uL is 0.00     NONE/BAD
 * f. (6) BV:Tet #2 = [DNA] ng/uL is 5, [DNA] ug/uL is 0.005   LOW/POOR
 * g. (7) BV:Tet/p22 #1 = [DNA] ng/uL is 5, [DNA] ug/uL is 0.005   LOW/POOR
 * h. (8) BV:Tet/p22 #1 = [DNA] ng/uL is 5, [DNA] ug/uL is 0.005   LOW/POOR
 * i. (9) BV:Tet/p22 #2 = [DNA] ng/uL is 335, [DNA] ug/uL is 0.335   HIGH/GOOD
 * j. (10) BV:Tet/p22 #2 = [DNA] ng/uL is 15, [DNA] ug/uL is 0.015   LOW/POOR
 * 2. Digest Rxn: Using #'s: 1, 2, 6, 9, and 10 from above. Also using: p22cII, LAMBDAcI, and RFP from different plasmid prep days. After following table below, allow to incubate for 2 hours. Heat inactivate enzymes @ 65C for 15 minutes in water bath. Follow the table below:
 * f. (6) BV:Tet #2 = [DNA] ng/uL is 5, [DNA] ug/uL is 0.005   LOW/POOR
 * g. (7) BV:Tet/p22 #1 = [DNA] ng/uL is 5, [DNA] ug/uL is 0.005   LOW/POOR
 * h. (8) BV:Tet/p22 #1 = [DNA] ng/uL is 5, [DNA] ug/uL is 0.005   LOW/POOR
 * i. (9) BV:Tet/p22 #2 = [DNA] ng/uL is 335, [DNA] ug/uL is 0.335   HIGH/GOOD
 * j. (10) BV:Tet/p22 #2 = [DNA] ng/uL is 15, [DNA] ug/uL is 0.015   LOW/POOR
 * 2. Digest Rxn: Using #'s: 1, 2, 6, 9, and 10 from above. Also using: p22cII, LAMBDAcI, and RFP from different plasmid prep days. After following table below, allow to incubate for 2 hours. Heat inactivate enzymes @ 65C for 15 minutes in water bath. Follow the table below:
 * i. (9) BV:Tet/p22 #2 = [DNA] ng/uL is 335, [DNA] ug/uL is 0.335   HIGH/GOOD
 * j. (10) BV:Tet/p22 #2 = [DNA] ng/uL is 15, [DNA] ug/uL is 0.015   LOW/POOR
 * 2. Digest Rxn: Using #'s: 1, 2, 6, 9, and 10 from above. Also using: p22cII, LAMBDAcI, and RFP from different plasmid prep days. After following table below, allow to incubate for 2 hours. Heat inactivate enzymes @ 65C for 15 minutes in water bath. Follow the table below:
 * j. (10) BV:Tet/p22 #2 = [DNA] ng/uL is 15, [DNA] ug/uL is 0.015   LOW/POOR
 * 2. Digest Rxn: Using #'s: 1, 2, 6, 9, and 10 from above. Also using: p22cII, LAMBDAcI, and RFP from different plasmid prep days. After following table below, allow to incubate for 2 hours. Heat inactivate enzymes @ 65C for 15 minutes in water bath. Follow the table below:
 * 2. Digest Rxn: Using #'s: 1, 2, 6, 9, and 10 from above. Also using: p22cII, LAMBDAcI, and RFP from different plasmid prep days. After following table below, allow to incubate for 2 hours. Heat inactivate enzymes @ 65C for 15 minutes in water bath. Follow the table below:


 * 3. Vector Dephosphorylate: Dephosphorylate the base vectors of the digested products. After following the table below, allow to incubate for 30 minutes @37C. Place samples in water bath @ 65C to deactivate the enzyme. Place samples in freezer @ -20C O/N to continue Ligation. Follow the table below:
 * 3. Vector Dephosphorylate: Dephosphorylate the base vectors of the digested products. After following the table below, allow to incubate for 30 minutes @37C. Place samples in water bath @ 65C to deactivate the enzyme. Place samples in freezer @ -20C O/N to continue Ligation. Follow the table below: