Team:Paris/July 14

Results of the transformation
Conclusion : Cells are competents, because there is positive transformation Hypothesis : problem with the protocol of DNA extraction from the registery 2008
 * Positive control : pUC19 (1µl; 10pg/ml) --> No transformation
 * Biobricks 2007 : --> succeed of the transformation unlike the delay added in the ice
 * Biobricks 2008 : --> No transformation

Culture of Cells

 * Culture of DH5ɑ in 5ml of LB without Antibiotics, O/N at 37°C

Preparation of medium and solutions

 * 1L of CCMB80
 * 500ml of SOB ( 2 x 250ml)
 * 500ml of SOC (2 x 250ml)
 * 100ml of MgCl2 (1M)
 * 150ml of CaCl2 (1M)
 * 200ml of KOAC (1M)