TUDelft/18 July 2008

=18th July 2008=

There were no colonies present on the plates. Because no positive control was performed, it is not clear what went wrong. Although it is clear that the heat shock was long for the cells used. Another transformation was started, this time with controls: pUC18 vector will be used as positive control for plasmid integration and a plate with just competent cells (no vector) as a negative control for working antibiotics. Another positive control was competent cells on a plate not containing any antibiotics. The plates contained 100 ug/ml Ampicillin. Again the GFP construct of the spots was used (well 4H of source plate 1004). Plates were incubated at room temperature over the weekend instead of at 37 oC o/n. Cells were transformed with approximately 100ng vector.