Team:Hawaii/Notebook/2008-08-29

= Things we did today =

PCR

 * Grace


 * nir and J33207 from plasmid preps
 * Verified products on 1.2% agarose gel

Plasmid preps

 * Grace


 * Resuspended plasmid preps in TE buffer
 * Verified preps on 1.2% agarose gel

Restriction digest

 * Grace


 * BB-pRL1383a and J33207 with XbaI and PstI
 * nir with EcoRI and SpeI
 * C0012 with EcoRI and PstI

Sequencing continued from 8-27

 * Margaret

{|class=wikitable border=1 align=center !construct !quantity(ng/ul)


 * Exo-Sap:OriV clones 1 thru 4, aadA(from BB registry) clones 5&9, aada(from pRL1383a) clones 3&6
 * OriV clones 1 thru 4, aadA(from BB registry) clones 5&9, aada(from pRL1383a) clones 3&6.
 * The omega interposon DNA was not submitted because there were multiple bands-->tomorrow I will work some more on this
 * also submitted some of Grace's constructs

Colony PCR

 * Margaret



= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"