Team:Mississippi State/Timeline of Things To Do

=Timeline of Things To Do=

A
 * 1) Order Supplies, most importantly the yeast vector(pPIC6alpha) and the gene(one of the LiP genes).
 * 2) Contact MSU person about possibility of getting LiP here
 * 3) Research other ways of obtaining the gene
 * 4) Center for Forest Mycology in Madison, WI has the organism and possibly the gene

B
 * 1) When we get supplies we need to begin by isolating LiP
 * 2) Transform into vector and measure expression
 * 3) Construct LiP BioBrick
 * 4) Transform into vector and measure expression
 * 5) Establish control system for expression
 * 6) Develop an entire system for controlable production and secretion of LiP

C
 * We should have the pPIC6alpha vector by Monday (June 16), along with the primers for the cDNA. Thus, we will do as follows:
 * 1) As soon as we get vector, transform into E-Coli for storage
 * 2) Make glycerol stock for storage of yeast
 * 3) PCR cDNA from P.C. to isolate LiPA using primers we have ordered.