Team:Paris/July 17

Results of the transformations (Biobricks 2007)
Legende :


 * few, medium, a lot --> description of the number of CFU (Colony forming unit) produce by the transformation
 * A: Ampicilin, K: Kanamycin

Cloning of the transformations

 * Test 8 clones by Biobricks
 * Incubate O/N at 37°C on proper plates

PCR Screening of the transformants
Use of the same 8 clones of Biobricks that have been tested for cloning

Protocol

 * 50µl of Mix PCR by tubes
 * 1 clone
 * Program : 105°C - for 29 cycles : 95°C; 5' - 95°C; 30" - 55°C; 30" - 72°C; 1,5'

Results

 * PCR 1 : J23109 (A)


 * PCR 2 : E0030 (K)


 * PCR 3 : pSB1A3 (A)


 * PCR 4 : C0179 (A)


 * PCR 5 : C0079 (K)


 * PCR 6 : R0079 (A)


 * PCR 7 : E1010 (K)


 * PCR 8 : B0034 (A)


 * PCR 9 : J23101 (A)


 * PCR 10 : E0040 (A)


 * PCR 11 : R0040 (A)