Brown: Team Resistance/27 September 2008


 * Miniprepped 15mL culture of 3:1 ligation.
 * Made a glycerol stock.
 * Restriction digest done of the ligation to make sure the ligation was successful.
 * Restriction Digest:
 * 1) 18 ul Water
 * 2) 25 ul DNA (82.5 ng/ul)
 * 3) 1 ul HindIII
 * 4) 1 ul EcoRI


 * Ran a gel of the digest with uncut DNA.
 * Results:
 * One band at approximately 5 kb
 * One band at 3-4 kb
 * A faint band possibly at <100 bp