Team:Caltech/Project/Vitamins/Parts

{{Caltech_iGEM_08| Content= Folate Production Parts

Basic Parts
{border="1" + Basic Parts (Extracted from L.lactis subspe. IL1403 genome) ! Part Name !! Registry # !! Description !! Cloned? !! Sequence confirmed? - ! Entire folate synthesis operon BBa_K137002 Includes folB+folKE+folP+ylgG+folC NO  NO - ! folB BBa_K137009 dihydroneopterin aldolase YES YES - ! folKE BBa_K137011 fusion gene: GTP cyclohydrolase & 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase  YES YES - ! folP BBa_K137012 Dihydropteroate synthase  NO NO - ! folC BBa_K137013 folate synthetase/polyglutamyl folate synthetase  NO NO - ! pabA BBa_K137005 para-aminobenzoate synthetase component II YES YES - ! pabB BBa_K137006 para-aminobenzoate synthetase component I YES YES - }

Construction Intermediates
{ border="1" + Construction Intermediates: Adding b0034 (strong RBS) before each individual gene ! Part Name !! Registry # !! Description !! Cloned? !! Sequence confirmed? - ! folB + b0034 BBa_S03957 RBS + dihydroneopterin aldolase YES YES - ! folKE + b0034 BBa_S03958 RBS + fusion gene: GTP cyclohydrolase & 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase  YES YES - ! folP + b0034 BBa_S03959 RBS + Dihydropteroate synthase  NO NO - ! folC + b0034 BBa_S03960 RBS + folate synthetase/polyglutamyl folate synthetase  NO NO - ! pabA + b0034 BBa_S03976 RBS + para-aminobenzoate synthetase component II YES YES - ! pabB + b0034 BBa_S03977 RBS + para-aminobenzoate synthetase component I YES YES - ! b0034 + folB + b0034 + folKE BBa_S03961 Combining folB (with RBS) + folKE (with RBS) YES YES - }

{ border="1" + Construction Intermediates: Adding j23100 (constitutive promoter) to each gene (with RBS already) ! Part Name !! Registry # !! Description !! Cloned? !! Sequence confirmed? - ! folB + j23100 BBa_S04032 Promoter(j23100) + RBS(b0034) + dihydroneopterin aldolase YES YES - ! folKE + j23100 BBa_S04033 Promoter + RBS + fusion gene: GTP cyclohydrolase & 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase  YES YES - ! pabA + j23100 BBa_S04034 Promoter + RBS + para-aminobenzoate synthetase component II YES YES - ! folBKE + j23100 BBa_S04035 Promoter + folB (with RBS) + folKE (with RBS) YES  YES - ! pabB + j23100 BBa_S04039 Promoter + pabB  YES  YES - ! pabB + b0015 BBa_S04038 pabB + b0015 terminator MAYBE  WAITING - }

Composite Parts
{ border="1" + Composite Parts: Adding b0015 (double terminator) to complete constructs with promoter + RBS already ! Part Name !! Registry # !! Description !! Cloned? !! Sequence confirmed? - ! folB + b0015 BBa_K137053 Promoter(j23100) + RBS(b0034) + folB (dihydroneopterin aldolase) + double terminator (b0015) YES  YES - ! folKE + b0015 BBa_K137054 Promoter + RBS + folKE (fusion gene: GTP cyclohydrolase & 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase) + double terminator (b0015)  YES  YES - ! pabA + b0015 BBa_K137055 Promoter + RBS + pabA (para-aminobenzoate synthetase component II) YES   YES - ! pabB + b0015 BBa_K137109 Promoter + RBS + pabB (para-aminobenzoate synthetase component I) YES   NO - ! folBKE + b0015 BBa_K137056 Promoter + RBS + folB + folKE + b0015 YES  NO - }

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