Team:Rice University/Notebook/7 June 2008

=Saturday 7 June= (from right to left, lane 1: 1Kb, lane 2: lambda DNA, lane 3: lambda with XhoI) <BR><BR> <BR><BR><BR><BR>
 * Selim Sheikh:
 * Prepared digestion of lambda DNA with XhoI only
 * Prepared gel electrophoresis of above digest along with solution of lambda DNA with 1Kb standard
 * Taylor Stevenson
 * Phage infected XL1-blue MR colonies cultured in 96 deep-well plate were spotted onto two EMB plates using a 2uL plate replicator. Both plates were incubated O/N, one at 30*C and one at 42*C (temp is high enough to denature the CI repressor, causing any lysogen to become lytic).
 * Result-both plates showed no bacterial growth after 24h.