Alberta NINT/30 May 2008

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lab work (SD):

DNA from LB + amp50 culture tubes (from 29/05/08) was isolated using QIA prep standard miniprep protocol. This DNA was set up for sequencing. K102002.4 becomes K102002 and K102004.4 becomes K102004.

lab work (JD):

Counted colonies on ligation plates: negative plate:0, Positive plate:2 because only two colonies grew we plated a second positive plate from our O/Ns and left it to grow

lab work (WM): Miniprepped 8 O/Ns Digested K102010/(EcoRI + PstI) Sequence with VR primer