TUDelft/15 September 2008

=September 15th=

Transformation
The DNA ligation over the weekend has been transformed into TOP10 made chemically competent.

Induction
To test for R0080 function, we're trying to visualize luciferase expression on an SDS-PAGE. Therefore we made a 1.33 M arabinose stock (1000x) for induction (page 3) and have grown K115012 and K115011 o/n both with and without induction.

Colony PCR E. coli genes
A colony PCR on eight colonies for atoB, idi and ispA were performed. The PCR was performed o/n, after the program the machine was programmed to keep a constant temperature of 4 degrees Celsius. The result will be checked on gel tomorrow.