Team:Mississippi State/Running a Gel

=Running a Gel=


 * Electrophoresis passes electricity through a sample of DNA or proteins and separates them by size. Therefore, you can see how much of certain size units are present in the sample.


 * 1) WEAR GLOVES!!!
 * 2) Weigh 0.25g Agarose into flask with 25ml 1xTAE Buffer, stirring
 * 3) Weigh, remember weight
 * 4) Microwave to boil several times
 * 5) allow to cool until just cool enough to hold in hand.
 * 6) Reweigh, adding ddH2O until original weight
 * 7) Add 5ul EtBr
 * 8) Pour in gel tray
 * 9) allow gel to set, with template in place
 * 10) remove template
 * 11) pour 1xTAE until full
 * 12) on parafilm, put 1ul drop of dye down for every sample.
 * 13) load 1ul DNA Ladder into first well
 * 14) Get 2ul of sample, mix with dye on parafilm
 * 15) Pipette mix into well
 * 16) Hook up Electrophoresis: Red(-) on right, black(+) on left, 80V voltage, for 60min
 * 17) When gel is done, turn off power, remove gel, wrap in Saran Wrap, take picture next door.