EPF-Lausanne/20 October 2008

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Cloning

 * Cloning of BC1 into AB
 * Using the simplified ligation protocol.


 * Cloning of CD into LuxI+ (LuxI with RBS and Term.)
 * Here a modified version of the ligation protocol was used, because the insert is quite big (over 2kb).
 * Reaction mix:
 * 3μl vector
 * 5.5μl insert
 * 1μl Ligase Buffer with ATP
 * 0.5μl T4 Ligase

=> Cloning worked.