Sequence

Sequencing Protocol
Protocol:

Go to Genewiz.com to order sequencing.

Sequencing primers should be diluted to 5uM; 4.0ul of 5uM primer should be used.

Depending on plasmid size and concentration, use up to 8ul of DNA; if less than 8ul, add autoclaved deionized water for volume.

Specifications According to Genewiz.com:

DNA Type/    DNA Size/     DNA Volume(total)/     Primer Volume (total) Plasmid/     <6kb/         8ul (~400ng)/          4ul (8pmol)

Plasmid/    6-10kb/      8ul (~600ng)/          4ul (8pmol)

Plasmid     >10kb/        8ul (~800ng)/          4ul (8pmol)

Purified PCR/    200-500bp/    8ul (3-10ng)/          4ul (8pmol)

Purified PCR/    .5-1kb/       8ul (5-20ng)/          4ul (8pmol)

Purified PCR/    1-2kb        8ul (10-40ng)         4ul (8pmol)