Team:NTU-Singapore/Notebook/26 August 2008



   =Tuesday 26 August= {|border="1" style="background-color:#ffffcc;" cellpadding="20"
 * Morning: Lu Chao: minipreps of 6 colonies inoculated on Monday and prepared 2% gel for later gel check.
 * Hung: digestional check: Results:
 * Among 4 samples of LsrA-RBS-Lysis-Term, 1 sample showed the correct bands: 2kb vector + 450bp insert (total length of LsrA+RBS+Lysis+Term). Our detection+lysis system is done!!
 * 2 samples of LacI-RBS-E7-Term showed wrong bands. However, ligation mixture for this was still not used up. We would transform the remaining mixture into top10 cells on Friday.
 * 2 samples of LacI-RBS-E7-Term showed wrong bands. However, ligation mixture for this was still not used up. We would transform the remaining mixture into top10 cells on Friday.