Team:Warsaw/Calendar-Main/25 August 2008

Tests for ampicillin resistance given by protein added to medium Piotr Purification of pACYC177+OmpA_alpha from antibiotic resistance contamination: Inoculation of E. coli TOP10 carrying pACTC177+OmpA_alpha to liquid LB with kanamycin and with kanamycin and ampicillin. Inoculation of TOP10 carrying pACYC177+OmpA_A_alpha and pACYC177+OmpA-alpha to LB with inductor (0.25 mM IPTG) and kanamycin.



Cloning of protein A DNA to pET15b+OmpA-alpha plasmid Antoni 

</li>  <a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of E. coli <a href="http://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain with <a href="http://2008.igem.org/Team:Warsaw/Calendar-Main/22_August_2008">friday's</a> ligation products. </li>  Transformants plating on LB + ampicillin.</li> </li>

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Cloning of alpha to <a href=http://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a> and <a href=http://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a> Michał K.  Eight colonies from each plate (<a href=http://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha-A-omega>pACYC177+OmpA_alpha_A_omega</a> and <A href=http://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BOmpA-alpha-Z-omega>pACYC177+OmpA_alpha_Z_omega</a>) were inoculated to liquid LB with kanamycin.