Team:Hawaii/Notebook/2008-06-16

= Things we did today =

Checked on plates in SC incubator

 * Grace


 * Checked on cell density and triparental conjugation plates
 * No cyanobacteria growth observed yet (they like to take their time). No contaminants observed.

Alkaline lysis prep

 * Grace & Krystle


 * Inoculated 5 ml LB+sp50 with 1 colony DH5&alpha; transformed w/ pRL1383a. Incubated overnight at 37C.

Restreaked DH5&alpha; w/ pRL1383a plate

 * Krystle


 * Restreaked on LB+sp100. Incubated at 37C overnight.

Completed Biosafety Training

 * Grace & Krystle

Primers

 * Krystle
 * Edited primers for nir promoter

Alkaline lysis

 * Grace
 * Researched protocols for alkaline lysis plasmid prep. Plan on doing plasmid prep tomorrow.

= Discussion =

= Quote of the Day = "Krystle: Well, you can have some stuff. Kevin (grad student): What stuff are you talking about? Krystle: Um... I was going to say some gummi bears... but we ate them all."