Team:Chiba/jk/β/week 1

>送受信班

>Protocols

17 August, 2008

 * Transformation
 * competent cells : XL10G
 * BBa_R0040(2008) -> No colonies on plates
 * BBa_C0070(2008) -> No colonies on plates
 * BBa_C0076(2008) -> No colonies on plates
 * BBa_C0077(2008) -> No colonies on plates
 * BBa_C0078(2008) -> No colonies on plates
 * BBa_C0079(2008) -> No colonies on plates

18 August, 2008

 * PCR
 * BBa_C0070(2008)
 * BBa_C0076(2008)
 * BBa_C0077(2008)
 * BBa_C0078(2008)
 * BBa_C0079(2008)
 * BBa_F2620(2007) ･･･control


 * 95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃


 * --->Gel Check


 * Transformation

competent cells : XL10G


 * BBa_C0070(2007)
 * BBa_C0070(2006)
 * BBa_C0076(2007)
 * BBa_C0076(2006) -> No colonies on plates(19/8)
 * BBa_C0078(2007)
 * BBa_C0078(2006)
 * BBa_C0170(2007)
 * BBa_C0170(2006)
 * BBa_C0178(2007)
 * BBa_C0178(2006)


 * --->(20/8)Mini prep

19 August, 2008

 * PCR
 * BBa_I9026(2007)
 * BBa_I9026(2006)
 * BBa_I9030(2007)
 * BBa_I9030(2006)
 * BBa_S3154(2007)
 * BBa_S3154(2006)
 * BBa_F2620(2007) ･･･control


 * 95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃


 * --->Gel Check

20 August, 2008

 * (18/8)--->Mini prep
 * BBa_C0070(2007)
 * BBa_C0070(2006)
 * BBa_C0076(2007)
 * BBa_C0078(2007)
 * BBa_C0078(2006)
 * BBa_C0170(2007)
 * BBa_C0170(2006)
 * BBa_C0178(2007)
 * BBa_C0178(2006)


 * --->Gel Check

--->Digestion test
 * BBa_C0170(2007)
 * BBa_C0170(2006)
 * BBa_C0178(2007)
 * BBa_C0178(2006)


 * --->Gel Check

21 August, 2008

 * (20/8)--->PCR
 * BBa_C0070(2007)
 * BBa_C0070(2006)
 * BBa_C0076(2007)
 * BBa_C0078(2007)
 * BBa_C0078(2006)


 * 95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃


 * --->Gel Check


 * Transformation
 * competent cells : XL10G
 * BBa_S03154(2007)
 * BBa_S03154(2006)
 * BBa_I9026(2007)
 * BBa_I9026(2006)
 * BBa_I9030(2007)
 * BBa_I9030(2006)

--->(23/8)Digestion

22 August, 2008

 * Transformation
 * competent cells : XL10G
 * BBa_J13002(2007)
 * BBa_J13002(2006)
 * BBa_J04500(2007)
 * BBa_J04500(2006)
 * (Locate & 2 Punch ~iyama ver.~)
 * BBa_C0076(2008) -> No colonies on plates
 * BBa_C0077(2008) -> No colonies on plates
 * BBa_C0078(2008) -> No colonies on plates
 * BBa_J04500(2008) -> No colonies on plates

--->(24/8)Mini prep

23 August, 2008

 * (21/8)--->Mini prep
 * BBa_S03154(2007)
 * BBa_S03154(2006)
 * BBa_I9026(2007)
 * BBa_I9026(2006)
 * BBa_I9030(2007)
 * BBa_I9030(2006)


 * --->Digestion test
 * BBa_S03154(2007)
 * BBa_S03154(2006)
 * BBa_I9026(2007)
 * BBa_I9026(2006)
 * BBa_I9030(2007)
 * BBa_I9030(2006)
 * BBa_J04500(2007)




 * --->Gel Check


 * Digestion test
 * BBa_J04500(2007:old-sample)

--->Gel Check