Alberta NINT/26 June 2008

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lab work (SD): Isolated DNA (K102008 ver 2) from 25/06/08 overnights. Set up K102008 ver 2 for sequencing. Digested K102008 ver 2 with EcoRI + PstI and ran on 2% agarose gel at 110V for 50 min. Excised fragments of K102008 ver 2 from the gel. Extracted DNA from the gel. Quantified K102002, K102008 and pSB4A5 from gel purified samples.

lab work (JD): Counted and Recorded colonies on plates from yesterday K102007(1)        120 Negative              200 K102007(2)        16 Negative                1 K102006               5 Negative                0 Obvious that K102007(1) didn't work but others seem like they might have :D Created 8 O/Ns of K102007(2) Created 5 O/Ns of K102006