Team:Davidson-Missouri Western/Las

Las/Rhl cell signaling system
Responsible: Robert Cool, Alicia Allen, and Erin Feeney

Las System

Signal Molecule: An AHL called PAI-1 (N-3-oxododecanoyl-l-hsl)(3-oxo-C12-hsl)

Bacterial species: Pseudomonas aeruginosa gram(-)  possibly E.coli (see article 3)

Receiver protein: LasR

Effect of binding: TXN activation of virulence genes, lasA, lasB, apr, toxR

Synthase: LasI enzyme

Target Genes: lasI, lasA, lasB, apr, toxR

Regulation: unknown

References

"Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes"

JP Pearson, EC Pesci and BH Iglewski 

"Regulation of Pseudomonas Quinolone Signal Synthesis in Pseudomonas aeruginosa"

Dana S. Wade, M. Worth Calfee, Edson R. Rocha, Elizabeth A. Ling, Elana Engstrom, James P. Coleman, and Everett C. Pesci 2

Posttranscriptional Control of Quorum-Sensing-Dependent Virulence Genes by DksA in Pseudomonas aeruginosa

Florence Jude,Thilo Köhler,Pavel Branny,Karl Perron,Matthias P. Mayer,Rachel Comte, and Christian van Delden 3

Pending:

Rhl System Signal Molecule: An AHL called PAI-2, Plasminogen activator inhibitor-2, N-butanoyl-homoserine lactone (C4HSL)

Bacterial species: Pseudomonas aeruginosa, gram(-)

Receiver Protein: Rhl R

Effect of Binding: activation of Rhamnosyl Transferase, then making RL (rhamnolipid)

Synthase: RhlA and RhlB

Target Genes: pqsABCDE and phnAB

Regulation: unknown

References

background information on Las and Rhl



Parts Needed:

LasR + pro/term

RhlR + pro/term

RhlI + pro/term

pqsABCDE + pro/term

pqsR

pqsH + pro/term

phnAB

LasI

RBS