Team:Warsaw/Calendar-Main/1 August 2008

Cloning of truncated fragment of protein A (&Delta;A) Michał K.   Colony PCR on pACYC177+OmpA_alpha + &Delta;A and pACYC177+OmpA_omega + &Delta;A colonies of tranformants.  Gel electrophoresis of PCR products (Fig. 1.).  

 Fig. 1. Colony PCR to find proper clones of pACYC177_OmpA_alpha+&Delta;A and pACYC177_OmpA_omega+&Delta;A Upper gel: 1. Marker 2-13. colony PCR products to find proper clone of pACYC177_OmpA_alpha+&Delta;A Lower gel: 1. Marker 2-11. colony PCR products to find proper clone of pACYC177_OmpA_omega+&Delta;A 12. positive control - pACYC_OmpA_A_alpha 13. negative control - pACYC_OmpA_Z_alpha