Team:Warsaw/Calendar-Main/9 September 2008

Purification of proteins: A-alpha Piotr

Measurement of concentration of protein(BCA method).

Tests for ampicillin resistance given by protein added to medium Piotr

Inoculation of TOP10 carrying pACYC177+OmpA_A_alpha, pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omega to LB with inducer (0.25 mM IPTG) and kanamycin.