Team:Mississippi State/Transformation of pPIC6alpha into E.Coli


 * The vector(We used pPIC6alpha A) comes in dry form, 20ug.
 * We added 20ul ddH2O to get stock solution of 1ug/ul of the vector.
 * Then, slow vortex followed by short spin.


 * 1) Add 100ul competent cell solution to 1.5ml tube.
 * 2) Add 0.2ul pPIC6alpha stock solution to cell stock in tube.
 * 3) Incubate on ice for 30min.
 * 4) Incubate in H2O @ 42C for 2min. (Heat Shock)
 * 5) Immediately add 500ul LB.
 * 6) Incubate at 37C with agitation for 1hr.
 * 7) Spread cells to 3 plates.
 * 8) Incubate at 37C for overnight(14-16hrs).