Team:The University of Alberta/Parts

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University of Alberta Parts Registry
All our submitted parts can be found in our section of the Parts Registry! More detailed documentation can be found there for each part.

Plastic Project

 * TetR + LVA tail|
 * PLACIQ + ERE brick - Sequence
 * Estrogen Receptor Alpha (Estrogen binding domain)
 * Estrogen Receptor Alpha (DNA binding domain)
 * BisdA BPA degredation bio brick - Sequence
 * BisdB BPA degredation bio brick - Sequence

Parts Ordered
PDF Sequences of synthetic genes ordered 20 Jun Genbank files synthetic genes ordered 20 Jun
 * [[Media:UA28-8genes-v2_(2).pdf]]
 * [[Media:iGEM08_BisDA.gb]]
 * [[Media:iGEM08_BisDB.gb]]
 * [[Media:iGEM08_ER01.gb]]
 * [[Media:iGEM08_ER02.gb]]
 * [[Media:iGEM08_LacIQ_ERE.gb]]
 * [[Media:iGEM08_RBS_6XHis.gb]]
 * [[Media:iGEM08_TDNA_MCS.gb]]
 * [[Media:iGEM08_TetR_RBS_6XHis.gb]]

Plant Parts



 * Plant CAMV 35S Promoter
 * Plant Resistance Brick
 * Chloramphenicol was used
 * IGEM Binary Vector
 * We constructed the IGEM binary vector using P0380 as a starting point. We removed the PUC8 Multiple cloning site from this binary vector and supplemented our own which will allow the vector to accept biobricks.
 * Blue OX, Purple Russian reporter systems

Butanol Parts
BioBricks Used This Summer


 * RBS + B-hydroxy butyryl CoA dehydrogenase (AKA. 21)
 * RBS + Enoyl-CoA hydratase (AKA. 22)
 * RBS + Butyryl CoA dehydrogenase (AKA. 23)
 * RBS + Butyraldehyde dehydrogenase (AKA. 24)
 * RBS + Butanol dehydrogenase (AKA. 25)
 * J61003 (without GFP)
 * I0500
 * pSB1A3

Parts Submitted


 * 21 + 22 (in plasmid pSB1A3)
 * 21 + 22 + 23 (in plasmid pSB1A3)
 * 23 + 24 (in plasmid pSB1A3)
 * 24 + 25 (in plasmid pSB1A3)
 * 21 + 22 + 23 + 24 + 25 (in plasmid pSB1A3)

Parts Ordered This Summer


 * ETFA (electron-transfer-flavoprotein, alpha polypeptide)
 * ETFB (electron-transfer-flavoprotein, beta polypeptide)

Parts Assembled This Summer For Protein Expression
 * J61003 + 21
 * J61003 + 22
 * J61003 + 23
 * J61003 + 24
 * J61003 + 25
 * I0500 + 21
 * I0500 + 22
 * I0500 + 23
 * I0500 + 24
 * I0500 + 25

For Butanol Operon
 * 21 + 22 (in plasmid pSB1A3)
 * 21 + 22 + 23 (in plasmid pSB1A3)
 * 23 + 24 (in plasmid pSB1A3)
 * 24 + 25 (in plasmid pSB1A3)
 * 21 + 22 + 23 + 24 + 25 (in plasmid pSB1A3)
 * ETFA + ETFB (in plasmid Puc57)