Team:The University of Alberta/23 July 2008


 * Gel purification of 1) conc. double digests of BisdA and BisdB with both (NgoMIV+Age1) and (XbaI+PstI); 2)conc. digest of vector containing the TetR binding site and the RBS with NgoMIV (digest was treated with Antartic Phosphatase); 3) conc. digest of J61003 with (SpeI+PstI) (digest was treated with Antartic Phosphatase).
 * Minipreped TetR O/N's set up on Tuesday night. It is in MininPrep Box 3.
 * Digested B0034 with Spe/Pst; TetR with XbaI/Pst. Digested the biobrick Lac1-ERE with Spe/Pst. Running them on 1% gel.
 * Gel purified the digestions.
 * Ligated TetR with RBS (B0034).
 * Digested pTet (R0400) with Xba/Pst.