Team:University of Ottawa/30 June 2008

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Today in the lab
Dan
 * Gel of friday-saturday ligation was run
 * <li> Ligation bands are very faint
 * <li> Decided to perfrom PCR cleanup on reaction mixture
 * <li>This time I used the correct amount all reactants
 * <li> Still, gel indicated no ligation after an hour
 * <li> WIll leave in PCR machine and check tomorrow
 * Digestion of 0
 * <li> Plasmid 0 containing our receptor was digested overnight with EagI in order to remove GFP which is already in the plasmid and will interfere with our system.