Alberta NINT/30 July 2008

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lab work (SD): Extracted DNA from gel using QIAquick gel extraction protocol. Quantified DNA and ligated K102031 /E+P and K102032 /E+P together. Transformed XL1-B cells with the ligation product.

lab work (JD): Ligation of K102011 with TAIn_2, TAIn_3, TAIn_6 and TAIn_7 Transformed XL1-B cells with the ligations Plated on LB+kan35 plates Incubated at 37C overnight