Alberta NINT/17 June 2008

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lab work (SD): Transformed XL1-B cells with: K102000 + I732018.1 K102000 + I732018.2 K102000 (negative control)

lab work (JD): Re-created K102003 with new LacZ parts I732018.1 and I732018.2 Ligated K102001 with LacZ Parts Transformed and plated XL1-B cells with K102003(1) (containing LacZ part I732018.1) and K102003 (2) (containing LacZ part I732018.2) Our plates of K102007 from yesterday with 1/10 1/100 and 1/1000 dilutions didn't seem to make any difference so we are going to recut TA0 Restriction digest of TA0 with BamHI and NcoI Nucleotide removal of TA0/B+N Created glycerol stock of K102005 Made fresh O/N of K102005 to be used tomorrow