Purdue/7 August 2008

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Jess digested o/n the mCherry parts to see if they're there...


 * 10uL DNA
 * 2uL Buffer #2
 * 8uL Mix (recipe below for 2 tubes)
 * 13uL ddH2O
 * 1uL EcoRI
 * 1uL SpeI
 * 1uL BSA


 * Put at 37C o/n
 * Ran on gel w/ Fast Blast
 * Found out that Fast Blast makes DNA Positive! (didn't work...)

Edited by Janie Stine