Team:ESBS-Strasbourg/16 July 2008

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DryLab
michi:
 * IRESes s*** let's mutate our promoter
 * last chance for IRES: wrote email to Pospisek
 * Calculated size of our constructs: 6 kb (too big for vector?)

Wiki

 * Further content in all categories (MM)

WetLab
Sandra: dh5a (Mariel) | pxj (10 ng)     | HeatShock (Maria) =      |      =           | KCM (Barbara) =      | LexA-BD (1 µl)   |      = =      | BB_pSB (2 µl)    |      = =      | BB_1763004 (2 µl)|      = dh5a (ours)  | pxj (10 ng)      | HS Top10         |     =            | = db3.1        |     =            | = =          | BB_p1010 (2 µl)  | = Mariel repeats the PCR from Paul from yesterday with 10ng plasmids =>Amplification of Gal4 succeed
 * Gel of the PCR from yesterday
 * Transformations
 * cells | part/vector | protocol

Paul: Katja:
 * Purification of Gal4 PCR product, stored at -20°C
 * Preparation of TENS buffer (not steril filtered)
 * Preparation of 3M NaAcO pH5
 * Miniprep with b.1) Protocol of the BBa_J63002(?) Plasmid to verify the identity by digestion after PCR failed

General
Our account is open again.(KK)