Team:Heidelberg/Notebook/Visualization/1stweek

Overview

=First week=

Monday 01/09/2008

 * Preparation of TB media with an Agar concentration of 0.4 %, 0.2 % and 0.1 %.

First microscope test using MG1655 in 6 small chambers from Labtek:

3 types each type twice: I - 0.1% agar in LB II - 0.25% agar in LB III - 0.5% agar in LB

View:

I II III I II III


 * In I and II each chamber was filled with just 100 µl LB agar to cover the whole ground.


 * Because the III. solution was more viscous 150 µl were necessary to cover the ground.


 * The bacteria were put into one corner of a chamber. (2 µl of a MG1655 overnight culture) They have been set under the media or in between.


 * About that another 100 µl of a LB solution with 2 % agar were poured. After the second layer has solidified further 250 µl LB-media were added.


 * The chambers were incubated overnight at 37 °C. For microscope on the next day.

Notes: Because of surface tensions the solutions tend to be thicker at the borders of the chamber than in the center. -> In the further future 150 - 200 µl will be used.

Sunday 07/09/2008
2ndWeek