Waterloo/20 June 2008

08/06/20 Friday

To do: [x] Make competent cells [x] Mini prep PluxR-gfp" [x] Diagnostic digest of mini preps (and run on gel)   - 1 + 1.5 hr - I think all transformants worked, but the ladder ran a bit weird, so we might have to check it again to make sure. [x] Repeat PluxRgfp" (1-8) with gfp`` positive control

[x] Prepare glycerol stock of registry parts [x] Patch streak inoculate of Registry transformations     - 1 hr? - will be taken out of 37 and put into 4 degree on saturday. [x] determined which registry parts we have from 2007, and box locations [x]diagnostic digest and gel of luxI and PlaclasI - inserts observed, but sizes are really off. - luxI`` (#1-4) wrong insert.