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pBca1256-K112600
Construction of {} basic part K112600

Wobble PCR SC001/SC002           (66 bp, EcoRI/BamHI) Sub into pBca1256                (EcoRI/BamHI, 2472+ 910) Product is pBca1256-K112600

SC001F   Forward Biobricking of  cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag SC002R   Reverse Biobricking of  ccagtGGATCCttcatgccattcaattttctgcgcttcaaaaatatcg

pBca1256-K112601
Construction of {} basic part K112602

Wobble PCR SC004/SC005           (45 bp, EcoRI/BamHI) Sub into pBca1256                (EcoRI/BamHI, 2472+ 910) Product is pBca1256-K112602 --- SC004    Forward Biobricking of  cgataGAATTCatgAGATCTgactacaaggatgacgacg SC005    Reverse Biobricking of  ccagtGGATCCcttgtcgtcgtcatccttgtagtc

pBca1256-K112603
Construction of {} basic part K112604

PCR mea035/sc007 on Bacillus amyloliquefaciens gen.           (320 bp, EcoRI/BamHI) Sub into pBca1256                                             (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112604 --- mea035   Fwd biobricking of {rbs_barstar>} cgataGAATTCatgAGATCTcataagaaaggagccgcacatg sc007    Reverse biobricking of {rbs_barstar>}                     (Not needed...) cgttaGGATCCagaaagtatgatggtgatgtcgc

pBjh1601AC-K112403
Construction of {PhoA>} basic part K112403 CR cb10007/cb10008 on MG1655           (95 bp, EcoRI/BamHI) Sub into pBjh1601AC                      (EcoRI/BamHI, 3195+910 L) Product is pBjh1601AC-K112403 - cb10007   Fwd biobricking of {prepro>} cgataGAATTCatgAGATCTatgaaacaaagcactattgcactggc cb10008   Reverse biobricking of {prepro>} cgtagGGATCCgggcttttgtcacaggggtaaac

pBjh1601AC-K112404
Construction of {LamB>} basic part K112404 PCR cb10009/cb10010 on MG1655           (107 bp, EcoRI/BamHI) Sub into pBjh1601AC                    (EcoRI/BamHI, 3195+910, L)                                               Product is pBjh1601AC-K112404 - cb10009   Fwd biobricking of {prepro>} cgataGAATTCatgAGATCTatgatgattactctgcgcaaac cb10010   Reverse biobricking of {prepro>} cgtagGGATCCcagccattgcctgagcagacattac

pBjh1601KC-K112605
Construction of {a~PhoA>} basic part K112605 PCR sc008/cb10008-phoA-R on MG1655           (100 bp, EcoRI/BamHI) Sub into pBjh1601KC                            (EcoRI/BamHI, 3132+910, L) Product is pBjh1601KC-K112605 -- sc008     Fwd biobricking of {a~prepro>} cgataGAATTCatgAGATCTtaaaGtgaaacaaagcactattgcactggc cb10008   Reverse biobricking of {a~prepro>} cgtagGGATCCgggcttttgtcacaggggtaaac

pBjh1601CK-K112606
Construction of {a~LamB>} basic part K112606 PCR sc009/cb10010-lamB-R on MG1655           (111 bp, EcoRI/BamHI) Sub into pBjh1601CK                            (EcoRI/BamHI, 3134+910, L) Product is pBjh1601CK-K112606 -- sc009     Fwd biobricking of {a~prepro>} cgataGAATTCatgAGATCTtagaatgatgattactctgcgc cb10010   Reverse biobricking of {a~prepro>} cgtagGGATCCcagccattgcctgagcagacattac

pBjh1601KC-K112607
Construction of {rbs_PhoA>} basic part K112607

PCR sc010/cb10008-PhoA-R on MG1655           (113 bp, EcoRI/BamHI) Sub into pBjh1601KC                            (EcoRI/BamHI, 3132+910, L) Product is pBjh1601KC-K112607 -- sc010     Fwd biobricking of {rbs_prepro>} cgataGAATTCatgAGATCTgtacatggagaaaataaaAtgaaacaaagcac cb10008   Reverse biobricking of {rbs_prepro>} cgtagGGATCCgggcttttgtcacaggggtaaac

pBjh1601KC-K112608
Construction of {rbs_LamB>} basic part K112608 PCR sc011/cb10010-lamB-R on E.coli K12 MG1655           (126 bp, EcoRI/BamHI) Sub into pBjh1601KC                                       (EcoRI/BamHI, 3132+910, L) Product is pBjh1601KC-K112608 -- sc011     Fwd biobricking of {rbs_prepro>} cgataGAATTCatgAGATCTcaatgactcaggagatagaatg cb10010   Reverse biobricking of {rbs_prepro>} cgtagGGATCCcagccattgcctgagcagacattac

pBjh1601CA-K112609
Construction of {a~PhoA>} basic part K112609 (K112605 replacement) PCR sc012/cb10008-phoA-R on MG1655           (100 bp, EcoRI/BamHI) Sub into pBjh1601CA                            (EcoRI/BamHI, 2472+910, L) Product is pBjh1601CA-K112605 -- sc012     Fwd biobricking of {a~prepro>} cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc cb10008   Reverse biobricking of {a~prepro>} cgtagGGATCCgggcttttgtcacaggggtaaac

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pBca1256-K112610
Construction of {PhoA>} basic part K112610 PCR cb10007/sc013 on MG1655                  (94 bp, EcoRI/BamHI) Sub into pBca1256                            (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112610 -- cb10007   Fwd biobricking of {prepro>} cgataGAATTCatgAGATCTatgaaacaaagcactattgcactggc sc013   Reverse biobricking of {prepro>} ccagtGGATCCggcttttgtcacaggggtaaac

pBca1256-K112611
Construction of {LamB>} basic part K112611 PCR cb10009/sc014 on MG1655                  (107 bp, EcoRI/BamHI) Sub into pBca1256                            (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112605 -- cb10009   Fwd biobricking of {prepro>} cgataGAATTCatgAGATCTatgatgattactctgcgcaaac sc014   Reverse biobricking of {prepro>} ccagtGGATCCagccattgcctgagcagac

pBca1256-K112612
Construction of {a~PhoA>} basic part K112612 PCR sc012/sc013 on MG1655                    (98 bp, EcoRI/BamHI) Sub into pBca1256                            (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112605 -- sc012     Fwd biobricking of {a~prepro>} cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc sc013   Reverse biobricking of {prepro>} ccagtGGATCCggcttttgtcacaggggtaaac

pBca1256-K112613
Construction of {a~LamB>} basic part K112613 PCR sc009/sc014 on MG1655                    (111 bp, EcoRI/BamHI) Sub into pBca1256                            (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112605 -- sc009     Fwd biobricking of {a~prepro>} cgataGAATTCatgAGATCTtagaatgatgattactctgcgc sc014   Reverse biobricking of {prepro>} ccagtGGATCCagccattgcctgagcagac

pBca1256-K112614
Construction of {rbs_PhoA>} basic part K112614 PCR sc010/sc013 on MG1655                    (112 bp, EcoRI/BamHI) Sub into pBca1256                            (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112605 -- sc010     Fwd biobricking of {rbs_prepro>} cgataGAATTCatgAGATCTgtacatggagaaaataaaAtgaaacaaagcac sc013   Reverse biobricking of {prepro>} ccagtGGATCCggcttttgtcacaggggtaaac

pBca1256-K112615
Construction of {rbs_LamB>} basic part K112615

PCR sc011/sc014 on MG1655                    (126 bp, EcoRI/BamHI) Sub into pBca1256                            (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112605 -- sc011     Fwd biobricking of {rbs_prepro>} cgataGAATTCatgAGATCTcaatgactcaggagatagaatg sc014   Reverse biobricking of {prepro>} ccagtGGATCCagccattgcctgagcagac

pBca1102-Bca9194 Assembly part
PCR ca998/mea037 on Bca9194                          (1610 bp, EcoRI/BamHI?) Sub into {} cgttaGGATCCCTTCAGGCCCAGCGCCGCTTTCATG

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