Team:Warsaw/Calendar-Main/6 June 2008

Blue/white and rifampicin test #1 Michał L., Ewa, Marcin Making chemocompetent E. coli TOP10 carrying pZC320.

Preparation of pMPMT5-AID+AIDT7 construct Michał K.  Isolation of hypothetical pMPM-T5-AID+AIDT7. Control Digest</a> of <A href=http://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID%2BAID-T7>pMPM-T5-AID+AIDT7</a> with BamHI (BamHI buffer).</li> DNA gel electrophoresis. Fig. 1</a></li></ol>

<img src="http://2008.igem.org/wiki/images/3/34/Trawienie_aat_kontrola_04_06_2008.jpg" width=250></a> Fig. 1. Control digest of the pMPMT5-AID+AIDT7 construct. Lane 2 - 5: isolated plasmids digested with BamHI