Alberta NINT/19 June 2008

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lab work (SD): Isolated DNA (K102002) from LB culture tubes from 18/06/08 using QIAprep spin miniprep protocol. Set up for sequencing.

lab work (JD): Counted and recorded number of colonies on newly plated K102007 plates from yesterday K102007 with the newly recut TA0: 128 K102007 with heated old TA0: 162 Negative control: 176 hence ligation didn't work AGAIN, tomorrow we will try something different and redo ligation on Monday Mini-prep of 7 O/N colonies of K102003.1-K102003.7 Set up for sequencing