Team:Hawaii/Notebook/2008-06- 4

= Things we did today =

Make Kan50 1000x Concentrate Stock

 * Krystle
 * Nanopure H2O + Kanamycin
 * stored in -20C freezer -> antibiotics box

Make Amp50, Amp50+Kan20 LB Plates

 * Krystle & Norman
 * made Kanamycin and Ampicillin LB plates according to Drew Endy's Protocol here
 * x10 Amp 50ug/mL
 * x10 Amp 50ug/mL + Kan 20ug/mL plates
 * left plates in hood overnight to dry after pouring (NW), please refrigerate plates in the morning.

Signal Peptide Research

 * Krystle
 * found two papers with leader sequences {please post on CiteULike}
 * Norman
 * blast sequence on Cu chealating protein, found PCC6803 gene and used SignalP 3.0 for signal peptide prediction

Discussion on CO2 Incubator Controller

 * Dr. Gautz, Krystle & Norman
 * KS will do some fact finding about the parts required
 * KS will put together schematic for CO2 regulator design

= Discussion =

= Quote of the Day =