Team:NTU-Singapore/Notebook/3 June 2008



   =Tuesday 3 June= {|border="1" style="background-color:#ffffcc;" cellpadding="20"

Chin Chong

 * Confirmed the orders for the oligo/primers designs to be purchase from 1st Base
 * List of things to purchase modified
 * Updated time-line for ordering chemicals and consumables
 * Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA

ZhenFu

 * Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA
 * Modified model to due to more estimation of parameters

Darius

 * Printed and pasted the Grand master plan of our project on the wall
 * Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA

Min Lin

 * Send out enquiries for potential sponsors
 * Waiting aproval for Proposal

Hung

 * Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA
 * Processing and storage of amplified DNA plasmids
 * Updated Wiki website

Choon Kit

 * Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA
 * Processing and storage of amplified DNA plasmids
 * Document laboratory reports for 02 Jun 2008

=Lab details=

Personnel
Incubate 4 tubes overnight
 * Hung,Choon Kit,Zhen Fu:
 * Morning:
 * Take out from the incubator 4 cryotubes. After spinning down, the tubes were observed:
 * 2 tubes with LBA contained no cell pellets at all as expected.
 * 2 tubes with LB without Amp. had cell pellets at the bottom. We then poured out the liquid and the tubes at -20 oC fridge for future use.
 * Take out from the incubator 5 agar plates. All had cell colonies growing on them except for LBA BW-W3110 plate. We stored them at 4 oC fridge.
 * Afternoon:
 * Inoculation to obtain 4 cryotubes:
 * 2 containing LBA with BBa_B0015 plasmid cells
 * 2 containing LBA with LacI-GFP plasmid cells