Team:Hawaii/Protocols/Transformation E coli

Protocol

 * 1) Thawed cells on ice.
 * 2) Transformed 50 &mu;l cells w/ 1 &mu;l DNA
 * 3) Incubated on ice 30 min.
 * 4) Heat shocked 60 sec. in 42C water bath
 * 5) Added 250 &mu;l SOC
 * 6) Incubated @ 37C for 1 hour with shaking (150 rpm)
 * 7) Plated 20-250 &mu;l onto LB+antibiotic plates
 * Plate more if needed.