Virginia/17 July 2008

Overnight Results

 * only plate with R0010 +A4 grew which is the same result as from the night before
 * Which leads us to think our assemblies have some problem
 * the assembly of BP2 and BP3 with the RBS(B0034) has failed twice in a row we need to look at the gel and make sure we are not making any false assumptions about the genes we have

Goals

 * Grow E0240 in liquid broth from originally growing plate that came from the new DNA spot in the most recent newsletter
 * we need to prove that this gene will glow green before we try to make it fluoresce in an assembly
 * Use overnight broth of A6(R0010 + A4) from night before to miniprep and grow in a LAC liquid culture
 * this should allow us to do an SDS-PAGE on this first bioplastic gene
 * we're not sure if a lack of terminator on the gene will be problematic
 * Digest and Assemble A6(R0010+A4) with a terminator(B0015) which we plan on also growing in a LAC liquid culture tomorrow
 * Grow the measurement plasmid(psb1a10) on a plate with arabinose and in overnight liquid broth without arabinose
 * plating with arabinose will prove that both genes in the vector will fluoresce
 * overnight broth will allow us to prepare DNA by miniprepping so that we can insert terminators in between fluorescence
 * Overnight broth with arabinose will be a double check to our work because we are not confident in our ability to make plates with arabinose
 * Try assembling and plating BP2 and BP3 with the medium strength RBS instead of standard
 * Start over with BP2 and BP3 grow a broth from the cells that were sent to us

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