Illinois/18 July 2008

LAB WORK

Receptor Tyrosine Kinase Method

competent cells:

1. 3mL overnight culture of DH5a pro

2. inoculated into 35mL of LB

3. OD600 checked, want 0.2-0.3

4. place culture on ice for 3 minutes

5. spin at 10,000 rpm for 7 minutes, discard supernatant

6. resuspend in 10 mL cold 30 mM CaCl2, incubated on ice overnight