Team:Hawaii/Protocols

List of Protocols
This is a list of tried and true protocols used by our team.

In vitro

 * Oligonucleotide hybridization
 * DNA ligation
 * DNA restriction digest
 * Gel extraction
 * ExoSAP

E. coli

 * Cryopreserve cells
 * Making competent cells
 * Transformation of  E. coli
 * Alkaline lysis / plasmid isolation
 * Colony PCR

Synechocystis sp. PCC6803

 * Triparental conjugation of Synechocystis sp. PCC6803

Media

 * Solid growth media
 * Liquid growth media
 * Antibiotics

BioBrick

 * BioBrick Construction Tutorial
 * Silver:BioBrick Strategy
 * Designing BioBrick Primers
 * BioBrick Part Fabrication