Template:Team:UC Berkeley/Notebook/CC construction

K112500
Construction of {} basic part K112500

Wobble PCR of cc001/cc002                 ( 50 bp, EcoRI/BamHI) Sub into pBca1256                         (EcoRI/BamHI, 2227+ 910,L) Product is pBca1256-K112500

cc001            Forward Biobricking of {} CgATAgaattcATGagatcttacccatacgacgtcccagac

cc002            Reverse biobricking of {} ccagtggatccccagcgtagtctgggacgtcgtatggg

K112501
Construction of {} CgATAgaattcATGagatcttacccatacgacgtcccagac

cc003            Reverse biobricking of {<HA!} ccagtggatccttaccagcgtagtctgggacgtcgtatgggtaag

K112502
Construction of { } basic part K112502

Wobble PCR of cc004/cc005                 ( 51 bp, EcoRI/BamHI) Sub into pBca1256                         (EcoRI/BamHI, 2227+ 910,L) Product is pBca1256-K112502

cc004            Forward Biobricking of { } CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG cc005            Reverse Biobricking of { } ccagtggatccCAGATCCTCTTCTGAGATGAGTTTTTG

K112503
Construction of {<myc!} basic part K112503

Wobble PCR of cc004/cc006                 ( 54 bp, EcoRI/BamHI) Sub into pBca1256                         (EcoRI/BamHI,2227+ 910,L) Product is pBca1256-K112503

cc004            Forward Biobricking of { } CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG cc006            Reverse Biobricking of {<myc!} ccagtggatccTTACAGATCCTCTTCTGAGATGAGTTTTTGTTC

K112504
Construction of { } basic part K112504

PCR CC007/CC008 on barnase gene           ( 361 bp, EcoRI/BamHI) Sub into pBca1256                         (EcoRI/BamHI, 2227+ 910,L) Product is pBca1256-K112504

cc007           Forward Biobricking of { } CGATAgaattcATGaGATCTGCACAGG CC008           Reverse Biobricking of { } CCAGTggatccTCTGATTTTTGTAAAGGTCTG

K112505
Construction of {<barnase!} basic part K112505

PCR CC007/CC009 on barnase gene           ( 364 bp, EcoRI/BamHI) Sub into pBca1256                         (EcoRI/BamHI, 2227+ 910,L) Product is pBca1256-K112505

cc007           Forward Biobricking of {<barnase!} CGATAgaattcATGaGATCTGCACAGG CC009           Reverse Biobricking of {<barnase!} CCAGTggatcCTTATCTGATTTTTG

K112232 (Molly's)
Construction of {rbs.barstar!} Biobrick Part K112232 PCR mea035/mea034 on Bacillus amyloliquefaciens gen         (320 bp, EcoRI/BamHI) Sub into pBca1256                                           (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112232

mea035          Forward Biobricking of {rbs.barstar!} cgataGAATTCatgAGATCTcataagaaaggagccgcacatg mea034          Reverse Biobricking of {rbs.barstar!} cgttaGGATCCttaagaaagtatgatggtgatgtcgcagcc

K112233 (Molly's)
Construction of {} Biobrick Part K112233 PCR mea036/mea037 on pBca9145-Bca1126		(1219 bp, EcoRI/BamHI) Sub into pBjh1601AC-Bca1144			(EcoRI/BamHI, 3195,910 L) Product is pBjh1601AC-K112233

mea036  Forward Biobricking of {}	cgatagaattcatgAGATCTGGAGATTCTG mea037  Reverse Biobricking of {}	cgttaGGATCCCTTCAGGCCCAGCGCCGCTTTCATG

K112506
Construction of {} basic part K112506

Wobble PCR of cc001/cc010                 ( 51 bp, EcoRI/BamHI) Sub into pBca1256                         (EcoRI/BamHI, 2227+ 910,L) Product is pBca1256-K112506

cc001            Forward Biobricking of {} CgATAgaattcATGagatcttacccatacgacgtcccagac

cc010            Reverse biobricking of {} ccagtggatcccccagcgtagtctgggacgtcgtatggg

K112507
Construction of {} CgATAgaattcATGagatcttacccatacgacgtcccagac

cc011            Reverse biobricking of {<HA!} ccagtggatccttacccagcgtagtctgggacgtcgtatgggtaag

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Cici Chen All notebook