User:University of Washington/4 August 2008

LuxR from pLac
-R0010 and E0240 ligation was transformed into DH5a strain.

-Original R0010 and E0240 ligation (from two? weeks ago) that had an inconclusive sequencing was sent in for sequencing again.

-Part I0462 (receieved from igem hq) was sent in for sequencing.

MG1655Z1
- MG1655Z1 from section 1 and 5 were miniprepped. Glycerol stock were made for both.

LuxR from AraC and TetR
- Ran gel for P1010 (AC x 3, Kan x 3) to combine the DNA into one tube.

- Restriction digest the P1010 only one on pSB1AC3, and the PCR Elowitz's promoter D29 with XbaI and PstI.
 * mixed two reaction
 * incubated 37 degree Celcius about 1 hour
 * 20 mins in 80 degree Celcius water bath
 * stored in the fridge

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