Team:Hawaii/Notebook/2008-10-23

= Things we did today =

Antibiotic test

 * Grace


 * No change from yesterday
 * Colony PCR of 6 colonies from sp40, 50, 75 and 8 colonies from sp100 plates
 * sp75,100 colonies correct
 * Restreaked sp2.5 blue colony, sp100 #1 & 2 on LB+amp100, LB, LB+sp100 + X-gal

Large scale plasmid prep

 * Grace


 * From sp100 plate: #1, 2
 * From 9/28 sp2.5 plate: #1
 * Gel showed no DNA in lanes migrated. Huh? Will try one more time to plasmid prep tomorrow.

Inoculated for plasmid prep

 * Grace
 * In 10 ml TB+amp100:
 * nir
 * nir+rbs
 * slr1
 * pilA
 * GFPf
 * slr1+GFPf
 * In 50 ml TB+sp100:
 * BB1 (sp100 plate)
 * Incubated at 37C for 18 hours with shaking at 250 rpm

Verification of transformants

 * Grace


 * Colony PCR of 4 BB-1 transformants
 * None were correct

RE digest

 * Grace


 * EcoRI/PstI: nir+rbs
 * XbaI/SpeI: slr1+GFPf

Plasmid Prep

 * Krystle
 * Plasmid prep of
 * lac+rbs+slr+gfpf #1,3,4
 * nir+rbs+slr+gfpf #2,3 (from last week)

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"