Team:Paris/August 16

=Construction of OmpR*+RBS and EnvZ*+RBS: '''Ligations=

Cleaning of the DNA after the digestion
We used the QIAcube to wash the DNA, following the standard protocol.

Measure of DNA concentration of the digestion products
We used the biophotometer. Settings:
 * 10 µL of template DNA in 50 µL of pure water
 * Blank : 10 µL of EB buffer in 50 µL of water.

List of ligations

 * We ligated the DNA following the standard protocol.
 * T5 is the autoligation control for L148 and L149.

=Creation of a registry of pFliL, pFlhDC, and FlhDC=

Analysis of the transformation we did yesterday
L143, L144, T1 and T2 showed no colonies. The positive control with pUC19 worked well. We suppose that the ligation did not work. We will do it again today.

Cleaning of the DNA after the digestion
We used the QIAcube to wash the DNA, following the standard protocol.

Measure of DNA concentration of the digestion products
We used the biophotometer. Settings:
 * 10 µL of template DNA in 50 µL of pure water
 * Blank : 10 µL of EB buffer in 50 µL of water.

List of ligations

 * We ligated the DNA following the standard protocol.
 * T1, T2, T3 and T4 are the autoligation controls for L 143, L144, L145 and L147

=Construction of pLas-TetR-GFP tripart & rbs-LasR-dble ter=

Transformation
Transformation protocol

Digestion check from yesterday
Protocol Digestion