Team:Hawaii/Notebook/2008-07-11

= Things we did today =

Plasmid prep of Biobricks

 * Krystle


 * Plasmid prep for B0015, E0026, R0010, J33207, B0030

Construction of pnir, slr2016, pilA with vector

 * Grace


 * Extracted DNA from gel using Qiagen MiniElute Gel Extraction Kit
 * Ligated pnir, slr2016, pilA with BBa_C0012 harboring vector
 * Ligated C0012 with vector as positive control
 * Ligated annealed products with vector
 * Ran ligations on gel
 * Annealed products + vector = smear
 * Rest of gel blank = not enough DNA

Made stock solutions

 * Grace


 * For plasmid prep:
 * GTE
 * NaOH/SDS
 * Potassium Acetate
 * General:
 * 0.25 M D-glucose
 * Autoclaved nanopure water

Made DB3.1 competent cells

 * Margaret

OriT Ligation Continued

 * Margaret


 * digested ligation with XbaI amd PstI, ran gel, cut it out (no picture). It is sitting in the 4&deg;C until Monday.

= Discussion =

= Quote of the Day =