Team:Paris/August 9

Measure of DNA concentration
First of all, we have to determine the concentration of DNA of the different templates.

We used the biophotometer.
 * Sample: 5µL of template DNA + 95µL of water
 * Blank: 5µL of EB buffer + 95 µL of water

Protocol of the ligation

 * 2 µL T4 Ligase Buffer 10X
 * X µg/µL vector
 * 3 or 4 x X µg/µL insert
 * Pure water qsp 20 µL
 * 1 µL T4 ligase
 * O/N at 16°C + sunday 16°C