Team:Chiba/jk/γ/week 3

実験ログ

出力班

31,August,2008
Digestion


 * BBa_R0010①
 * PCR product(BBa_J52008)②

-->37℃ 3hour

-->①20μl refine

-->②20μl refine

1,September,2008
Ligation
 * vector:BBa_R0010 insert:BBa_J52008①
 * negative control:BBa_R0010②

-->R/T 2hour

Transformation


 * BBa_R0079(Las promoter)
 * BBa_R0071(RhlR promoter)
 * BBa_R0077(cinR promoter+RBS)
 * BBa_R0078(cinR promoter)
 * BBa_R0062(Lux promoter)

2,September,2008
TIME RESPONCE (Solid)

Colony PCR
 * BBa_R0010+BBa_J52008

Mini prep
 * BBa_R0079
 * BBa_R0071
 * BBa_R0077
 * BBa_R0078
 * BBa_R0062

3,September,2008
Digestion test
 * BBa_R0079①
 * BBa_R0071②
 * BBa_R0077③
 * BBa_R0078④
 * BBa_R0062⑤

-->37℃30min

-->Gel check

4,Septempber,2008
Transformation


 * Ligation product
 * rLUciferase(BBa_J52008+BBa_F2620 )  x3
 * Venus YFP(BBa_E2030+BBa_F2620)   x3

5,September,2008
TIME RESPONCE(liquid)

Digestion test
 * BBa_J52008+BBa_F2620 x2
 * BBa_E2030+BBa_F2620 x2

-->37℃ 30in

Gel Check
 * BBa_J52008+BBa_F2620 x2①
 * BBa_E2030+BBa_F2620 x2②
 * BBa_J52008(PCR Product)③
 * BBa_E2050④


 * Luciferase check-->no light

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