User:University of Washington/2 July 2008

BioBrick Promoter Measurements
- The four overnight cultures of TOP10 cells containing promoter constructs I20260, I20268, I20269, and I20270 were centrifuged at 14,000 RPM for 3 minutes. Then, the supernatant was decanted out, leaving only pelleted cells.

- The pelleted cells were miniprepped using a Qiagen miniprep kit.

- 4 uL of each plasmid sequence was added to its own two tubes. One tube had 8 uL of forward primer added to it, and the other had 8 uL of reverse primer added to it. These reaction tubes were submitted to the UW Sequencing Facility for sequencing.

- The remaining purified plasmid solutions were stored at -20 degrees Celsius.

LuxR from pLac
- I0462 transformed cells failed to grow on Amp plate. I0462 has now failed to transform three times!

- The I0462 DNA spot from the notebook was nanodropped to verify that it actually contains DNA. It did indeed contain DNA: 55.8 ng/ul, 1.25 (260/280), 1.30 (260/230).

- Part I763004 was transformed into DH strain, in substition for defective part J04430.

LuxR from AraC and TetR
- Nanodrop AraC: 162.5 ng/ul, 1.93 (260/280), 1.47 (260/230)

Induction of Conjugative Transfer (Bryan & Scott)
- Designed and ordered primers for Lambda Red recombination of RP4 promoter sequences

Team:University_of_Washington/Notebook