Team:Edinburgh/ISO2

= ISO2 =

Amino Acid Sequence
MASSLPAPPASPSSSWRGLTPRCPPPRCGPLLARAAARSYRYRFRTDDDG VVDVAVAGKDGDAGYVVAIEAPTHGQRGGLVLRPAGSGEGVPLAPAAPGG ALVAELSYDVARAPFHVSFTLADAMGAEIRTHRGTSFRVPVGVGRGCPSP LGLSQSKDGAANFAVYSKIAKGMVLCLFGGGGGDGPALEIELDPYVHRTG DVWHVSMESVEGYARYGFRSGLFAMFGIDRPLLDPYAKVIGDFVAGDSVD EDGLAVPSIRCLASLKNAPNYDWGRDKHPCLPLQKLVVYRANVALFTKDR SSRLADNAAGTFSGMSAKVEHFRHLGVNAVLLEPVFPFHQVKGPYFPYHF FSPMSLYSSECSSVSAIKSMKDMVKTMHRNGIEVLLEVVFTHTAEGGAEC QMISLRGIDGSSYYIADGIAGCKASVLNCNHPVTQKLILDSLRHWVLDFH VDGFCFINAPFLVRGPGGEGLSRPPLLEAIAFDPVLSKTKIIADPWSPLD ISNVQFPFPHWKRWAEMNTRFSMDVRKFLKGEALISDLATRLCGSGDLFS SRGPAFSFNYVSRNSGLTLVDLVSFSSDELASEFSWNYGEEGPSENNAVF QTRLRQIHNFLFILFISLGIPVLNMGDECGNSAAGSTSYKDRGLLNWKAL KTAFVKEVTGFISFLSALRSRRADIFQRCEFLKLENIHWYGSDLSEPCWE DPTSNFLCLHINAELDEKLPDSTGGDLYICFNANEESASATLPAIAEGSM WLRLVDTSLAFPGFFSRGSSHETHQVLGFSSYQVKAHSCVLFESKRVLS*

DNA Sequence
atggcctcctccctccccgcgccgccggcctcgccctcttcctcctggcg 50 accggaggagggaggggcgcggcggccggagcgggagaaggaggaccgc cggactcacgccccgctgccctccgcctcgctgcggtcccctcctcgccc 100 gcctgagtgcggggcgacgggaggcggagcgacgccaggggaggagcggg gcgcggcagcgcgttcttaccgttaccgcttccgaaccgacgacgacggc 150 cgcgccgtcgcgcaagaatggcaatggcgaaggcttggctgctgctgccg gtggtggacgtggccgtcgccgggaaagacggcgatgcggggtatgtggt 200 caccacctgcaccggcagcggccctttctgccgctacgccccatacacca cgctatcgaggctcctacccatggacagaggggcggtcttgtgctccgcc 250 gcgatagctccgaggatgggtacctgtctccccgccagaacacgaggcgg ccgccggctccggcgagggcgtccctctggccccagccgcgccgggaggt 300 ggcggccgaggccgctcccgcagggagaccggggtcggcgcggccctcca gccctcgtggctgagttgtcctacgacgtggcccgcgcgccgttccacgt 350 cgggagcaccgactcaacaggatgctgcaccgggcgcgcggcaaggtgca ctcgttcacgctggccgacgcgatgggagcggagatacggacacaccgcg 400 gagcaagtgcgaccggctgcgctaccctcgcctctatgcctgtgtggcgc ggacgagcttccgcgtgcctgttggcgtcggacggggctgcccctcgccg 450 cctgctcgaaggcgcacggacaaccgcagcctgccccgacggggagcggc ctcggcctgtcccagtccaaggatggggccgctaacttcgcggtttacag 500 gagccggacagggtcaggttcctaccccggcgattgaagcgccaaatgtc caagatcgccaagggcatggtgctctgcctcttcggtggtggcggcgggg 550 gttctagcggttcccgtaccacgagacggagaagccaccaccgccgcccc acggacccgcgcttgagattgagctcgacccgtacgtccaccggaccggc 600 tgcctgggcgcgaactctaactcgagctgggcatgcaggtggcctggccg gatgtctggcacgtctcgatggagagcgtggaggggtacgcccgctacgg 650 ctacagaccgtgcagagctacctctcgcacctccccatgcgggcgatgcc cttccgcagcgggctgttcgcaatgtttggcattgaccgcccgctactcg 700 gaaggcgtcgcccgacaagcgttacaaaccgtaactggcgggcgatgagc acccgtacgccaaggtgatcggggacttcgtcgctggcgactctgttgat 750 tgggcatgcggttccactagcccctgaagcagcgaccgctgagacaacta gaggatgggctagctgtgccatccataaggtgtctcgcgtccttgaagaa 800 ctcctacccgatcgacacggtaggtattccacagagcgcaggaacttctt tgcacccaactacgattggggcagggacaagcacccatgcttgccattgc 850 acgtgggttgatgctaaccccgtccctgttcgtgggtacgaacggtaacg agaagctggtggtctaccgggcaaatgtagctttgttcaccaaggatagg 900 tcttcgaccaccagatggcccgtttacatcgaaacaagtggttcctatcc tcgagtcggctggcagacaatgccgctggtactttctccggcatgtctgc 950 agctcagccgaccgtctgttacggcgaccatgaaagaggccgtacagacg aaaggtggaacacttcaggcatcttggtgtcaatgcagttttgctggagc 1000 tttccaccttgtgaagtccgtagaaccacagttacgtcaaaacgacctcg cagttttcccattccaccaagtgaagggaccatattttccataccatttt 1050 gtcaaaagggtaaggtggttcacttccctggtataaaaggtatggtaaaa ttttcacctatgagcttgtatagcagtgaatgctccagtgtttcagctat 1100 aaaagtggatactcgaacatatcgtcacttacgaggtcacaaagtcgata caagtctatgaaggatatggtcaaaacaatgcacagaaatggaatagagg 1150 gttcagatacttcctataccagttttgttacgtgtctttaccttatctcc ttctcttggaggttgttttcacgcatactgctgaaggaggggcggagtgt 1200 aagagaacctccaacaaaagtgcgtatgacgacttcctccccgcctcaca cagatgatatcacttcgaggcatcgatggttcctcgtactacattgctga 1250 gtctactatagtgaagctccgtagctaccaaggagcatgatgtaacgact tggaatcgctggatgcaaggcaagtgtgttgaattgcaaccatccagtga 1300 accttagcgacctacgttccgttcacacaacttaacgttggtaggtcact ctcagaagctgattttggacagcctccgccattgggtgctcgacttccat 1350 gagtcttcgactaaaacctgtcggaggcggtaacccacgagctgaaggta gttgatgggttctgcttcatcaatgctcctttcctcgtcagaggtccagg 1400 caactacccaagacgaagtagttacgaggaaaggagcagtctccaggtcc tggtgagggcctctcacggcctccacttctggaagccatagcatttgatc 1450 accactcccggagagtgccggaggtgaagaccttcggtatcgtaaactag ctgttctttcaaagactaagatcattgcagatccttggtctccgcttgac 1500 gacaagaaagtttctgattctagtaacgtctaggaaccagaggcgaactg atatctaatgtgcaatttccattccctcattggaaaagatgggctgagat 1550 tatagattacacgttaaaggtaagggagtaaccttttctacccgactcta gaacacaagattctctatggatgtgcgcaagtttcttaagggagaagcac 1600 cttgtgttctaagagatacctacacgcgttcaaagaattccctcttcgtg ttatcagtgatcttgctacacgtttgtgtggcagtggggacttattttcc 1650 aatagtcactagaacgatgtgcaaacacaccgtcacccctgaataaaagg tcaaggggcccagcattttcgttcaattatgtatccaggaattctggact 1700 agttccccgggtcgtaaaagcaagttaatacataggtccttaagacctga cactcttgttgatctagtgagcttcagcagtgatgagcttgcttctgagt 1750 gtgagaacaactagatcactcgaagtcgtcactactcgaacgaagactca tcagctggaattatggtgaagaaggaccatcggagaacaacgcagtcttt 1800 agtcgaccttaataccacttcttcctggtagcctcttgttgcgtcagaaa caaaccaggctaagacagatacacaacttcctgtttattctattcatttc 1850 gtttggtccgattctgtctatgtgttgaaggacaaataagataagtaaag ccttggtattcctgttcttaacatgggggatgaatgtggaaactcagctg 1900 ggaaccataaggacaagaattgtaccccctacttacacctttgagtcgac ctggttcaacatcatacaaggatagagggcttctgaactggaaagccttg 1950 gaccaagttgtagtatgttcctatctcccgaagacttgacctttcggaac aagaccgcttttgttaaggaagttaccgggtttatttcgtttctatctgc 2000 ttctggcgaaaacaattccttcaatggcccaaataaagcaaagatagacg actaaggagtcgacgagcagacattttccagagatgcgagtttctaaaac 2050 tgattcctcagctgctcgtctgtaaaaggtctctacgctcaaagattttg ttgaaaatatacattggtatgggagtgatttatctgagccatgttgggag 2100 aacttttatatgtaaccataccctcactaaatagactcggtacaaccctc gatcctactagcaactttctttgcttgcacataaatgcagagctggacga 2150 ctaggatgatcgttgaaagaaacgaacgtgtatttacgtctcgacctgct gaagctaccagattcgactggaggtgatttgtatatctgtttcaatgcaa 2200 cttcgatggtctaagctgacctccactaaacatatagacaaagttacgtt acgaggagtcagcgagtgctactttaccagcaattgcagaaggatccatg 2250 tgctcctcagtcgctcacgatgaaatggtcgttaacgtcttcctaggtac tggctgcgcttggttgatacatcacttgcatttccaggtttcttttccag 2300 accgacgcgaaccaactatgtagtgaacgtaaaggtccaaagaaaaggtc agggtctagtcatgaaacacaccaggtgctaggattttcctcatatcaag 2350 tcccagatcagtactttgtgtggtccacgatcctaaaaggagtatagttc tgaaggcacatagctgtgttttgttcgaatccaagagggttctttcatag 2400 acttccgtgtatcgacacaaaacaagcttaggttctcccaagaaagtatc

EcoRI FOUND 1.

BioBrickTM Primers
Forward: gat gaattc gcggccgc t tctag atg gcc tcc tcc ctc cc

Reverse: gct actagt a tta tta tga aag aac cct ctt gg

Mutagenic Primers
1689-1694 EcoRI (Asn AAT --> AAC in forward primer)

Forward (1692-1708):  c tct gga ctc act ctt g Reverse (1691-1674): tt cct gga tac ata att g