Protocols Ligation

Cocktail: 5.5ul d.s.H2O 1ul Backbone (keep on ice) 2ul Insert (keep on ice) 1ul Buffer (T4 DNA buffer) 0.5ul Ligase (T4 DNA ligase) == Negative Control 1 == == Negative Control 2 == == Control for PNK == Let stand at room temperature for 20 minutes
 * Use 2mM Tris-Cl in place of insert
 * Use 2mM Tris-Cl in place of backbone
 * Use 2ul of d.s. H2O instead of insert

Protocols