Alberta NINT/29 May 2008

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lab work (SD):

Colony PCR of 9 colonies from K102002 (XL1-B) and 9 colonies from K102004 (XL1-B) (plates from 28/05/09). DNA was run on 2% agarose gel. All 9 samples were identical on the gel. K102002.2, K102002.4, K102002.5, K102002.8, K102004.1, K102004.3, K102004.4, and K102004.5 were selected at random, inoculated into LB + amp50 culture tubes and incubated overnight at 37 C.

lab work (JD):

Gel purified R0011 band from gel electrophoresis. Carried out quantitative analysis Carried out ligation to create K102001 (R0011+T/A1) Transformed XL1-B cells with K102001. Prepared R0011 for sequencing and dropped it off.

lab work (WM): # colonies from K102010 ligation: ~100       negative ctl: 0 setup 8 O/Ns