Team:Edinburgh/bglX

= bglX =

AMINO ACID SEQUENCE
MKKITVLISIWLSAAAFFSCEKKTEAGSKPQAFDKEVQDLLKNMSLEEKAGQMTQIDI

RNLLNNGYGNTDEKLDTARLKEAIQTYHVGSILNCIQAYTPEKWVELISQIQNEALQS

PNKIPVLYGTDAMHGVGFIKDAVLFPHNIGMAASRNDQLVSQAAQVTSTEARSVGLTW

NFAPVLDVGREPYWSRFEETFGEDVYITTQMGSAAVQMMEGSDLTSKTNIASCLKHFI

GYSAPKNGIDRTQSHIPEIVLREYYLPPFREAINKGASSIMINSAEINGIPCHGNKWL

LTDLLRTELGFTGMVVSDWEDVIRLHTWHKVAATPKEAVMMAVNAGVDMSMVPNDYSF

PKYLVELVKEGKVSMARIDEAVGRILTLKIKLGLMKNPLPSIADVGVVGSDAHQQIAL

NAARESITLLKNDKNILPLAKDKKILLVGPAANSLSALHSSWSYTWQGSNESLYPETT

KTIREALEASGNKANIRTNATTGFDDAANYDVSFIQKNTAGVDVIIVCVGEAAYAEQP

GVIKDLNLPEAQKQLIVAAKKTGKPVIVCLVEGRPRLFPEEEALADAVIMCYRPGSKG

ADAFAEILYGDINPSGKLPFTYPRYDGDITTYDYKFKETEQQLKPGVSEFVAFNPQWP

FGHGLSYTTFAYSNLNVNKSNFTKNDSVLVTVDISNTGARTGKIAVELYSRDHFASIT

PSERRLRKYTKIELKAGEKQTVSFTIKAADLQFVNKDLKTVTEAGAFDLMIGNLQTEI

YFNE

DNA SEQUENCE
atgaaaaaaataaccgtattgatttccatctggctcagtgcagccgcttt 50

tactttttttattggcataactaaaggtagaccgagtcacgtcggcgaaa

tttctcttgtgagaaaaaaacagaagcggggagtaaacctcaggcctttg 100

aaagagaacactctttttttgtcttcgcccctcatttggagtccggaaac

ataaagaagtacaggatctgttaaaaaatatgtcgctggaagaaaaagcc 150

tatttcttcatgtcctagacaattttttatacagcgaccttctttttcgg

ggacagatgacgcagatcgatatccgtaatttattgaacaacggatatgg 200

cctgtctactgcgtctagctataggcattaaataacttgttgcctatacc

aaataccgatgaaaaattagataccgccagattaaaagaagccattcaaa 250

tttatggctactttttaatctatggcggtctaattttcttcggtaagttt

cctatcatgtcggttctatattaaattgcattcaggcatacacacctgaa 300

ggatagtacagccaagatataatttaacgtaagtccgtatgtgtggactt

aaatgggttgaattgatttctcagatccagaacgaagcattgcaatcacc 350

tttacccaacttaactaaagagtctaggtcttgcttcgtaacgttagtgg

taataaaattccggttttatacggaacagatgccatgcatggtgttggct 400

attattttaaggccaaaatatgccttgtctacggtacgtaccacaaccga

ttattaaagatgccgtattgtttccgcataacataggtatggctgcatcc 450

aataatttctacggcataacaaaggcgtattgtatccataccgacgtagg

cgcaacgatcaattggtttcgcaggcagctcaggtaacgtcaacagaagc 500

gcgttgctagttaaccaaagcgtccgtcgagtccattgcagttgtcttcg

gcgttccgttggccttacctggaattttgctccggtgcttgacgtaggca 550

cgcaaggcaaccggaatggaccttaaaacgaggccacgaactgcatccgt

gagaaccttactggtcgcgctttgaagaaacctttggtgaagatgtatac 600

ctcttggaatgaccagcgcgaaacttctttggaaaccacttctacatatg

ataacaacacaaatgggttcagcagccgtacaaatgatggaaggcagtga 650

tattgttgtgtttacccaagtcgtcggcatgtttactaccttccgtcact

cctgacgtctaaaacaaacatagcttcctgcctgaaacactttattggat 700

ggactgcagattttgtttgtatcgaaggacggactttgtgaaataaccta

attcagcacccaaaaatggtatagaccgcacacaatcacacattcctgaa 750

taagtcgtgggtttttaccatatctggcgtgtgttagtgtgtaaggactt

attgtgctgcgcgaatattatctgcccccgttcagagaagcgatcaataa 800

taacacgacgcgcttataatagacgggggcaagtctcttcgctagttatt

aggcgcttcatccattatgatcaactctgctgagatcaatggcattccgt 850

tccgcgaagtaggtaatactagttgagacgactctagttaccgtaaggca

gtcacggcaacaaatggctgttaacagatctgctccgtacagagcttgga 900

cagtgccgttgtttaccgacaattgtctagacgaggcatgtctcgaacct

tttaccggaatggttgtatcggattgggaagatgtgatccgtttgcacac 950

aaatggccttaccaacatagcctaacccttctacactaggcaaacgtgtg

ctggcataaagtagctgctacgccgaaagaagccgtaatgatggccgtta 1000

gaccgtatttcatcgacgatgcggctttcttcggcattactaccggcaat

acgcaggtgtggatatgagcatggtgccgaatgattactcgtttccgaaa 1050

tgcgtccacacctatactcgtaccacggcttactaatgagcaaaggcttt

tatctggtagaattggtgaaagaaggaaaagtttctatggcgcgcatcga 1100

atagaccatcttaaccactttcttccttttcaaagataccgcgcgtagct

cgaagcggtgggacgtattctgaccctgaaaatcaaactgggcttgatga 1150

gcttcgccaccctgcataagactgggacttttagtttgacccgaactact

aaaatccattgccatccattgcagatgttggtgtagtgggttctgatgca 1200

ttttaggtaacggtaggtaacgtctacaaccacatcacccaagactacgt

catcaacagatcgcactaaatgcagcgcgtgaatcaattacattattgaa 1250

gtagttgtctagcgtgatttacgtcgcgcacttagttaatgtaataactt

gaacgataaaaatattctgcctcttgcaaaagataaaaaaatactattgg 1300

cttgctatttttataagacggagaacgttttctatttttttatgataacc

ttggtccggccgctaacagtttatctgcgctgcacagttcatggtcatac 1350

aaccaggccggcgattgtcaaatagacgcgacgtgtcaagtaccagtatg

acatggcagggaagcaatgaatcgctgtatcctgaaacaactaaaacaat 1400

tgtaccgtcccttcgttacttagcgacataggactttgttgattttgtta

ccgtgaggcattagaagcgagcggtaataaagctaacatcagaacgaatg 1450

ggcactccgtaatcttcgctcgccattatttcgattgtagtcttgcttac

cgactacaggatttgatgatgcagcaaattatgatgtttcctttattcaa 1500

gctgatgtcctaaactactacgtcgtttaatactacaaaggaaataagtt

aaaaatacagcaggggtggatgtaattattgtgtgtgtaggtgaagctgc 1550

tttttatgtcgtccccacctacattaataacacacacatccacttcgacg

ctatgccgaacagcctggagtaatcaaggatctcaatctgccggaagcgc 1600

gatacggcttgtcggacctcattagttcctagagttagacggccttcgcg

agaagcaattgattgttgctgcaaagaaaacaggcaaacctgtcattgta 1650

tcttcgttaactaacaacgacgtttcttttgtccgtttggacagtaacat

tgcctggtagagggcaggccgcgtttgtttccggaagaagaagcacttgc 1700

acggaccatctcccgtccggcgcaaacaaaggccttcttcttcgtgaacg

agatgctgtaataatgtgctaccgtccgggcagcaaaggtgcagacgcat 1750

tctacgacattattacacgatggcaggcccgtcgtttccacgtctgcgta

ttgcagagattctatacggtgacatcaatcccagcggtaagcttccgttc 1800

aacgtctctaagatatgccactgtagttagggtcgccattcgaaggcaag

acctatccgagatacgacggagatattactacatacgattataaatttaa 1850

tggataggctctatgctgcctctataatgatgtatgctaatatttaaatt

ggaaaccgagcagcagttaaaaccgggcgtgagtgaatttgttgcgttca 1900

cctttggctcgtcgtcaattttggcccgcactcacttaaacaacgcaagt

atccgcaatggccattcggacatggcttgagctacacaacgtttgcatat 1950

taggcgttaccggtaagcctgtaccgaactcgatgtgttgcaaacgtata

tcaaaccttaacgtgaacaaaagtaattttacgaagaacgatagtgtgct 2000

agtttggaattgcacttgttttcattaaaatgcttcttgctatcacacga

ggtaacggtagacataagcaatacaggagcacgtacaggtaagatagccg 2050

ccattgccatctgtattcgttatgtcctcgtgcatgtccattctatcggc

tagagttatattccagagaccacttcgcatcgattacgccttctgaaaga 2100

atctcaatataaggtctctggtgaagcgtagctaatgcggaagactttct

cgcttacgtaaatacacaaagattgaactgaaagcaggagagaagcaaac 2150

gcgaatgcatttatgtgtttctaacttgactttcgtcctctcttcgtttg

cgtttcgtttacgatcaaagcagcggatctgcaatttgtaaataaagatc 2200

gcaaagcaaatgctagtttcgtcgcctagacgttaaacatttatttctag

tgaaaacagtaaccgaagcaggcgcttttgatctgatgatcggaaatctc 2250

acttttgtcattggcttcgtccgcgaaaactagactactagcctttagag

cagacagaaatatattttaatgagtaa 2277

gtctgtctttatataaaattactcatt

Forbidden Restriction Sites: None!

BIOBRICKTM PRIMERS
Forward: CGT gaattc gcggccgc t tctagATG AAA AAA ATA ACC GTA TTG

Reverse: CGT t actagt a TTA TTA CTC ATT AAA ATA TAT TTC TG