EPF-Lausanne/6 August 2008

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Miniprep of last part that had failed: I1466

Digested E1010 and F1060 and purified for ligation

Simulation: Did a little. We have to find a way to minimise feedback.

Research hasn't yet found anything good. Looking at a few ideas: -Protease to degrade when state has been chosen -RhlR mutation -low detect to build in a time delay.