Team:Cambridge/Signaling/Constructs

Lowercase letters are biobrick ends, uppercase are the actual pieces.

AgrA
atgaattcgcggccgcttctagagATGGAAATCGCACTGGCTACGGACAATCCATATGAGGTTCTGGAACAGGCTAAAAACATGAACGACATCGGCTGCTATTTCCTGGATATTCAGCTGAGCACCGACATCAACGGTATCAAACTGGGCTCTGAAATCCGTAAACATGATCCGGTTGGTAACATTATTTTCGTGACTTCTCATTCTGAGCTGACCTATCTGACGTTCGTATACAAGGTTGCAGCCATGGATTTCATCTTTAAAGACGATCCGGCAGAGCTGCGTACGCGCATCATCGACTGTCTGGAAACTGCGCACACGCGCCTGCAACTGCTGTCCAAAGATAACTCCGTGGAAACCATTGAACTGAAACGTGGCAGCAACAGCGTTTACGTTCAGTACGATGATATCATGTTCTTCGAAAGCTCCACTAAGTCCCACCGTCTGATCGCGCATCTGGATAATCGTCAGATCGAGTTCTATGGCAACCTGAAAGAGCTGAGCCAGCTGGACGATCGCTTCTTCCGTTGTCATAACTCCTTCGTTGTTAACCGTCACAACATCGAATCCATCGACTCCAAAGAGCGTATTGTATACTTCAAGAACAAAGAACACTGTTATGCGTCTGTACGCAATGTCAAGAAAATCTAAtactagtagcggccgctgcaggcggc

AgrB
atgaattcgcggccgcttctagagATGAACTATTTTGACAACAAGATCGACCAATTTGCTACTTACCTGCAAAAACGCAACAACCTGGACCACATCCAGTTTCTGCAAGTTCGTCTGGGCATGCAGGTTCTGGCTAAAAACATCGGCAAACTGATCGTTATGTACACCATCGCCTATATTCTGAACATCTTCCTGTTTACCCTGATCACCAACCTGACCTTCTATCTGATTCGCCGTCATGCCCACGGCGCTCATGCACCTTCTTCCTTTTGGTGTTATGTAGAGAGCATTATTCTGTTTATCCTGCTGCCGCTGGTAATCGTTAACTTCCACATCAATTTCCTGATTATGATCATCCTGACTGTGATTTCCCTGGGTGTAATCTCTGTTTACGCTCCGGCTGCCACTAAGAAAAAGCCGATTCCGGTGCGCCTGATCAAACGTAAAAAGTACTACGCTATCATCGTTTCCCTGACCCTGTTCATTATCACCCTGATTATCAAAGAACCGTTTGCACAGTTCATCCAGCTGGGTATTATCATCGAAGCGATCACTCTGCTGCCGATCTTTTTCATCAAAGAGGATCTGAAATAAtactagtagcggccgctgcaggcggc

AgrC
atgaattcgcggccgcttctagagATGATTCTGATGTTCACCATTCCAGCAATTATTAGCGGTATTAAGTACAGCAAGCTGGATTATTTCTTCATCATCGTAATCTCTACCCTGTCCCTGTTCCTGTTCAAAATGTTCGATTCCGCGAGCCTGATTATCCTGACTTCCTTCATCATCATCATGTATTTCGTAAAAATCAAATGGTATAGCATCCTGCTGATCATGACCTCCCAGATTATCCTGTACTGTGCCAACTACATGTATATCGTTATCTACGCGTACATCACGAAAATCTCCGATTCTATCTTCGTTATCTTTCCGTCTTTCTTCGTGGTGTACGTTACGATCTCTATTCTGTTTTCCTATATTATCAATCGCGTTCTGAAAAAGATCTCTACTCCGTACCTGATTCTGAACAAAGGTTTTCTGATCGTCATCTCCACTATCCTGCTGCTGACCTTCTCTCTGTTCTTCTTTTACTCTCAGATCAACTCCGATGAGGCAAAAGTCATCCGTCAGTACAGCTTCATCTTTATCGGTATTACTATCTTCCTGAGCATTCTGACTTTCGTTATCAGCCAGTTCCTGCTGAAAGAAATGAAGTACAAGCGCAACCAAGAAGAAATCGAGACTTACTACGAGTACACCCTGAAGATTGAAGCGATCAACAACGAAATGCGTAAATTTCGTCACGACTACGTTAACATTCTGACCACCCTGAGCGAATACATCCGTGAAGACGACATGCCTGGCCTGCGTGATTATTTCAACAAAAACATTGTGCCGATGAAAGATAACCTGCAAATGAACGCTATTAAGCTGAACGGTATCGAAAACCTGAAAGTACGTGAGATCAAGGGTCTGATTACCGCTAAAATCCTGCGTGCGCAGGAAATGAACATCCCAATTAGCATCGAAATCCCAGACGAAGTTTCTAGCATTAACCTGAACATGATTGACCTGTCTCGTAGCATCGGCATCATCCTGGACAACGCTATCGAAGCTTCCACTGAAATCGACGACCCGATTATTCGCGTTGCTTTTATCGAGAGCGAAAACTCCGTGACCTTTATCGTGATGAACAAATGCGCGGACGATATCCCGCGTATTCACGAACTGTTCCAGGAATCTTTCAGCACCAAGGGTGAAGGTCGCGGCCTGGGCCTGTCCACCCTGAAAGAGATCGCGGATAACGCCGACAACGTTCTGCTGGACACTATTATCGAAAACGGTTTCTTCATTCAGAAAGTCGAAATCATCAATAACTAAtactagtagcggccgctgcaggcggc

AgrD
atgaattcgcggccgcttctagagATGAATACTCTGTTCAATCTGTTTTTCGATTTCATTACTGGCATCCTGAAGAACATCGGTAACATCGCAGCGTACAGCACCTGCGACTTCATCATGGATGAAGTAGAGGTCCCGAAAGAGCTGACCCAGCTGCATGAATAAtactagtagcggccgctgcaggcggc

pXyl Promoter
With BB sites: 163 Without: 115 atgaattcgcggccgcttctagagGAAAAACTAAAAAAAATATTGAAAATACTGATGAGGTTATTTAAGATTAAAATAAGTTAGTTTGTTTGGGCAACAAACTAATGTGCAACTTACTTACAATATGACATAAAATGCAtactagtagcggccgctgcagatc

pSpac Promoter
With BB sites: 155 Without: 107 atgaattcgcggccgcttctagagAGAACAACCTCTGCTAAAATTCCTGAAAAATTTTGCAAAAAGTTGTTGACTTTATCTACAAGGTGTGGCATAATGTGTGGAATTGTGAGCGGATAACAATTAAGCTTtactagtagcggccgctgcagata

pPac Promoter
With BB sites: 125 Without: 75 atgaattcgcggccgcttctagagAAACGAGGTCATCATTTCCTTCCGAAAAAACGGTTGCATTTAAATCTTACATATGTAATACTTTCAAAGACTACAtactagtagcggccgctgcaggcggc

pUpp Promoter
With BB sites: 285 Without: 239 gaattcgcggccgcttctagagATTTTTAAAGTATGTATACAAATGATGAATAAATTTTGGCGATATAATGAAGGATACAGCTCCCATAATTGGTAAAGATACTAGATAGATTCATCGTAAAATCATGATTTTGCCAAATTTGCCCTTGAATATTAGTAGCGTTTTCTTTACAATCGTAAATAGTGTAAAAAAGCGTGCAAACGCATGAATATCATCTAAAGGAGAGATTCACATGGGAAAACTGTATGTATTTGATCCTCtactagtagcggccgctgcagat

consensus B. subtilis RBS
With BB sites: 54 Without: 11 gaattcgcggccgcttctagagAAAGGAGG TGTtactagtagcggccgctgcag

weak B. subtilis RBS
With BB sites: 54 Without: 11 gaattcgcggccgcttctagagAGAGGTGG TGTtactagtagcggccgctgcag