Team:Hawaii/Notebook/2008-09-30

= Things we did today =

Plasmid Prep

 * Grace


 * Plasmid prep of BBpRL1383a #1, 3, 13, 18

Construction of secretion device (cont.)

 * Grace
 * PCR:
 * nir+rbs
 * slr1+GFPf
 * plac+rbs
 * rbs+GFP
 * nir
 * nir+rbs+pilA #17 (yet to be confirmed via sequencing)
 * rbs+GFPf+tt #1, 2 (yet to be confirmed via sequencing)
 * GFPf+tt #21
 * Overnight RE digest:
 * EcoRI/PstI:
 * pSB1A3
 * EcoRI/SpeI:
 * nir
 * rbs+GFP
 * nir+rbs
 * plac+rbs
 * slr1+GFPf
 * nir+rbs+pilA #17
 * XbaI/PstI:
 * slr1+GFPf
 * rbs+GFPf+tt #1, 2
 * GFPf+tt #21
 * rbs+GFP

Plasmid Prep

 * Margaret


 * pSB1A3, plac/rbs/rep + pSB1A3 (also made a glycerol stock of this), and oriT in pSB1A3

RE-digest from yesterday

 * Margaret


 * gel purification of aadaBB, aadApRL, and oriV
 * de-phosphorylate pSB1A2, pSB1A3a, pSB3K3

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"