Team:Mississippi State/Maintenance of Plasmids

=Maintenance of Plasmids=


 * The pPIC6alpha vector contains the blasticidin resistance gene to allow selection of the plasmid using blasticidin. To propogate and maintain the pPIC6alpha plasmid, use the following procedure:
 * 1) Resuspend each vector in 20ul sterile water to prepare a 1ug/ul stock solution. Store the stock solution at -20C
 * 2) Use the stock solution to transform a recA, endA E.coli strain like TOP10, DH5alpha, JM109, or equivalent.
 * 3) Select transformants on Low Salt LB plates containing 100ug/ml blasticidin.
 * 4) Prepare a glycerol stock from each transformant containing plasmid for long-term storage.