Team:Mississippi State/28 July 2008


 * 1) prepare 500uM stock solution of primers
 * 2) prepare working stock of primers
 * 3) prepare PCR mix: 40ul ddH2O, 5ul PfxBuffer, 1ul dNTP, 1ul Up2, 1ul Down2, 1ul RP#1, 1ul Pfx50
 * 4) Make low salt LB plates
 * 5) perform PCR purification using Qiagen kit
 * 6) prepare and run 1% agarose gel
 * 7) pour plates
 * 8) Transform pPIC6 into ecoli
 * 9) perform digestion of Purified PCR with EcoRI and NotI
 * 10) Prepare and run 1% agarose gel
 * 11) cut out gel and perform gel extraction using Qiagen kit