Team:Warsaw/Calendar-Main/11 September 2008

Tests for ampicillin resistance given by protein added to medium Piotr

Inoculation of pACYC177+OmpA_variants from previous day to LB + 0.25 mM IPTG, kanamycin and 50 μl/ml ampicillin with minimum concentration of prey (yesterday's results). Measurement of growth after 6 hours - 2-fold growth increase in presence of interacting proteins (pACYC177+OmpA_A_omega twice as much as pACYC177+OmpA_omega with protein Z_alpha, pACYC177+OmpA_Z_omega twice as much as pACYC177+OmpA_omega with protein A_alpha and pACYC177+OmpA_Z_alpha twice as much as pACYC177+OmpA_alpha with protein Z_omega.)!!!!</li></ol>

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