Imperial College/10 August 2008

= 10 August 2008 =

''B.subtilis
Team members - James and Krupa + more if work load is too much.

Monday

 * Prepare an overnight culture of DL1-blue E.coli with the pDR110 integration vector,
 * Prepare an 2x overnight culture of B.subtilis,
 * Prepare the reagents required for transformation,

Tuesday

 * Miniprep of the XL1-blue overnight culture,
 * Make competent cells for both of the transformation protocols

Wednesday

 * Perform transformation of the competent cells using both protocols,

Thursday

 * Check successfulness of the transformation. If successful then perform transformation of linear DNA using previously made competent cells.

Friday

 * Check successfulness of the linear DNA transformation,

Produce cell number against OD600nm calibration curve for use in characterisation

Cloning
Chris and Tom for this week

Monday

 * Design all primers for PCR cloning

Tuesday

 * Transform XL1-blue E.coli with Biobricks containing BBa_B0015, BBa_C0012 and pSB1A3

Wednesday to Friday

 * PCR clone AmyE from pDR111 and XylR from the B.subtilis genome when primers arrive
 * Prepare ALL remaining Protocols
 * Depending on arrival of primers, connect XylR and BBa_C0012 to BBa_B0015 (separately) to begin construction of inducible promoters

Microscope

 * Prepare B.subtilis for first microscopy session on thursday - a set of dilutions to ascertain optimal amount of cells per mL
 * Chris, James, Prudence and Clinton will attend microscope training

End of week five
Finish tutorial 2:
 * Maximum Likelihood Estimation
 * Moments
 * The Bayesian Approach

Next week

 * Start working on tutorial 3 (hypothesis testing)
 * Generate data sets from bacteria motility movies

Thursday

 * Attend microscope training (Clinton and Prudence)