Team:Warsaw/Calendar-Main/24 July 2008

Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA Paweł Results of ligation: 6 colonies grown. Each colony cultured overnight in LB + ampicillin.

Cloning of protein A DNA to OmpA constructs Michał K.  Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega. Primers used:

AL+SacI and AP+NotI.</li> Gel electrophoresis.</li> Confirmed transformant colonies inoculated to liquid LB with kanamycin. </li></ol>

Preparation of chemocompetent bacteria Antoni

Preparation of <a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemocompetent">chemocompetent</a> bacteria E. coli <a href="http://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htmtop10">TOP10</a>.