Team:Mississippi State/Isolated Vector


 * 1) After centrifuge, empty supernatant into flask.
 * 2) Resuspend pellet in 250ul pl buffer.
 * 3) Vortex until cell pellet dissolves(looks milky.)
 * 4) Add 250ul p2 buffer, mix thoroughly by INVERTING 4-6 TIMES!!, don't vortex.
 * 5) Immediately add 350ul N3 buffer, mix immediately by inverting 4-6 times, mix thoroughly.
 * 6) Centrifuge 10min @ 13000rpm (17900g.)
 * 7) Get 3 spin columns from kit.
 * 8) Put supernatant into spin column via pipette.
 * 9) Centrifuge 30-60s, discard flow-through.
 * 10) Wash spin column, add 0.5ml PB buffer and centrifuge 30-60s, discard flow-through.
 * 11) Wash spin column, add .75ml PE buffer, centrifuge 60s.
 * 12) Discard flow-through, centrifuge additional 1min to remove residual wash buffer.
 * 13) place QIAprep column in clean 1.5ml centrifuge tube. To elate DNA, add 50ul water to the center of each QIAprep spin  column, let stand for 5-10min, centrifuge 1min.
 * 14) Run Gel.