Team:Hawaii/Protocols/Antibiotics

Ampicillin
1000X ampicillin stock is a 100 mg/ml solution.


 * 1) Combine 1 g ampicillin with 10 ml nanopure H2O.
 * 2) Sterile filter using a 0.2 &mu;m Corning filter.
 * 3) Aliquot into eppendorf tubes and store at -20C.

Kanamycin
1000X kanamycin stock is a 50 mg/ml solution.


 * 1) Combine 0.5 g kanamycin with 10 ml nanopure H2O.
 * 2) Sterile filter using a 0.2 &mu;m Corning filter.
 * 3) Aliquot into eppendorf tubes and store at -20C.

Spectinomycin
1000X spectinomycin stock is a 100 mg/ml solution.


 * 1) Combine 1 g spectinomycin with 10 ml nanopure H2O.
 * 2) Sterile filter using a 0.2 &mu;m Corning filter.
 * 3) Aliquot into eppendorf tubes and store at -20C.

Streptomycin
1000X streptomycin stock is a 50 mg/ml solution.


 * 1) Combine 0.5 g streptomycin with 10 ml nanopure H2O.
 * 2) Sterile filter using a 0.2 &mu;m Corning filter.
 * 3) Aliquot into eppendorf tubes and store at -20C.