Virginia/13 August 2008


 * Made small broth of A58-A60
 * Not sure about A58 and A59 - few colonies, satellites
 * Made small broth of re-streaked B0034
 * Still not confident in this
 * Digested I715039 with E,S and B1002 with E,X to create RFP + term ligation
 * Cooled off butanolysis apparatus, will do extraction soon
 * Extracted with 3ml MilliQ water. Saved top organic layer in vials labeled 'butanolysis product'
 * Bought 1ml (5 * 0.2ml) of Library Efficiency DH5a cells (Invitrogen)

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