Imperial College/22 August 2008

= 22 August 2008 =

B.subtilis

 * The transformation protocol 1, | click here for link to the protocol, was checked today. The plate containing B.subtilis transformed with no DNA had no colonies, B.subtilis transformed with 40ng DNA contained ~10 colonies and B.subtilis transformed with 400ng DNA contained ~10 colonies.

Cloning

 * Double digest results from the 21st checked. A second gel was visualised early (but no image recorded) to allow identification of the small Termiantor insert
 * Insert was succesfully identified on the gel
 * All Registry sequences that were succesfully cloned appear to be ok for use