Team:Hawaii/Notebook/2008-10- 1

= Things we did today =

Construction of secretion device (cont.)

 * Grace




 * Ran RE digest products on gel
 * nrp #17 band did not cut
 * plac+rbs band not visible on gel
 * GFPf+tt #21 band did not cut
 * Extracted bands from gel
 * Ligated:
 * nir & rbs+GFP
 * nir+rbs & slr1+GFPf
 * slr1+GFPf & tt
 * rbs+GFP & tt
 * nir & rbs+GFP+tt #1
 * plac & rbs+GFP+tt #1
 * nir & rbs+GFP+tt #2
 * plac & rbs+GFP+tt #2
 * plac & rbs+GFP
 * rbs + slr1+GFPf
 * pSB1A3 to self (control)
 * SAP treated pSB1A3 to self (to test for SAP/buffer contamination)
 * Used 5 &mu;l ligation reaction to transform DH5&alpha; cells

Made LB+Amp100 plates

 * Grace

Made Amp100 1000X stock solution

 * Grace

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"