User:University of Washington/21 August 2008

LuxR from AraC and TetR(Faifan)
- nanodropped the gel purified DNA at 230 nm

- ligation
 * mix
 * 1 hr incubate at room temp
 * 15 mins denature at 65 degree Celsius
 * 20 mins filtration
 * transformed 2ul DNA into XL1-Blue, inoculated on Kan plate

MG1655Z1(Faifan)
-None of the four cultures grew on Tet, Kan, Cam

-Only culture#3 didn't grow on Amp
 * Streaked #3 to new Tsy plate
 * grew overnight of #3 in Tsy

LuxR from pLac
--I-insert and R-vector parts were isolated from their gel fragments and ligated together.

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