Team:NTU-Singapore/Notebook/18 June 2008



  

=Wednesday 18 June= {|border="1" style="background-color:#ffffcc;" cellpadding="20" These tasks are carried out for E7 insert and empty plasmid vector for RBS 0034:
 * 4:30 pm: 1st digestion
 * 6:30 pm: PCR purification, followed by 2nd digestion
 * 11:30 pm: collect digested product and freeze at -20 to prevent reaction.
 * Also, at 6:15pm, we plate LacI promoter in Homemade Top 10 and DH5α