Team:NTU-Singapore/Notebook/4 June 2008



  

=Wednesday 4 June= {|border="1" style="background-color:#ffffcc;" cellpadding="20"

Personnel
Choon Kit, Hung, Darius:
 * Morning:
 * Take out 4 cryotubes (2 terminators, 2 LacI-GFP) from yesterday incubation, then spin them down, pour out the liquid to obtain cell pellets.
 * Prepare 6 digestion solutions for gel electrophoresis in the afternoon:
 * 2 for terminators
 * 2 for LacI-GFP
 * 1 for CoE7 plasmid from BW strain (from -20 oC fridge)
 * 1 for CoE7 plasmid from W3110 strain (from -20 oC fridge)


 * Afternoon:
 * Amplification for the following plasmids (from Registry):
 * GFP generator BBa_E0240
 * RBS BBa_B0032 and RBS BBa_B0034
 * T7 promoter BBa_I719005
 * Run gel for morning samples.
 * Inoculation of BW (2x),W3110 (2x), MG1655 (2x) for glycerol stock on Thursday.
 * Prepare LB broth
 * Prepare 17x LBA agar plates