Team:Warsaw/Calendar-Main/28 July 2008

Cloning omega-A fusion on pKS (second attempt) Michał L., Ewa, Marcin Sequencing confirms that we have obtained construct with omega-A fusion, but A in our constructs turned out to be truncated and contain only one B-domain (which we called 'delta A'). We inoculated bacteria containing this plasmid to obtain it for futher cloning.

Purification of proteins: Z-alpha and Z-omega

Piotr Single transformations of Rosetta with plasmids pET15b+Z-alpha and pET15b+Z-omega. Plating them on LB with chloramphenicol and ampicillin.