Illinois/10 June 2008

iGem Meeting Minutes 6/10/2008

The meeting began at 3:10

3:10 Aleem gave the presentation we made yesterday 4:00 Dr. Rao will take 4 people into his lab and teach them how to clone DNA 4:40 We decided who was going to be in each group. The groups are posted on the Powerpoint. If you weren't at the meeting pick a group to work with and contact the group members.
 * Advice from Professors (Rao and Golding):
 * try expressing a T-cell receptor in yeast
 * try using an FC receptor attached to an antibody-allows it to be generic and usable with various antibodies
 * Potential Challenges:
 * Get yeast--ask Dr. Orleans. He is in India for a month but go to his lab. Another option is Dr. Zhao
 * We need a pheremone for the schmoos to come together
 * Can we buy a pheremone? we should be able to, but look into details.
 * We need a reporter
 * GFP or lacZ(colorimetric reaction). lacZ maybe a better choice.
 * To Do this week and early next week:
 * Get yeast and begin culturing--it usually takes 2 days at room temperature in YPD media.
 * Get powdered media and pour it into dishes
 * Order NEB catalog
 * See what the yeast stock center in Seattle, has to offer
 * They should have free fluorescent proteins
 * Dr.Rao explained the procedure for Tuesday's experiment
 * Step 1 amplification-PCR
 * Step 2 separation-gel electrophoresis
 * Step 3 cut plasmid using restriction enzymes
 * Step 4 add gene A by binding sticky ends with DNA ligase

There is additional info and pictures describing what Dr. Rao explained today at the end of the powerpoint.

The meeting ended at 5:00.