Team:Hawaii/Notebook/2008-06- 5

= What we did today =

Made PCR gel

 * Norman
 * 12g Agarose
 * 12 ml 50X TAE
 * 588 ml nanopure water
 * 6 &mu;l EtBr

Transformed DH5&alpha; with PRL1383a

 * Grace & Krystle
 * Used same protocol as competency test on 6/3/08
 * Shaking at 235 rpm
 * Plated on LB+sp plate
 * E. coli from batch 2 (2-14) (OD600=0.3)

= Discussion =
 * GK and KS will read up on PCC6803 transformation tonight; transformation will be conducted tomorrow morning.
 * Tomorrow afternoon, we will practice extracting BioBrick plasmids off filter paper.
 * Also need to make TE tomorrow.

= Quote of the day = "So Grace, what promoter are you going use?"
 * "Um... my name is Krystle."

(an hour later, via Skype)

"Okay, see you all next week. Bye Grace... er... Margaret."