Team:Johns Hopkins/Protocols

Protocols
[[Media:JHU_0708_Jaime-protocols.pdf|PCR Protocols from Jaime]] [[Media:JHU_0708_IGEM_Ligation_and_Transformation.pdf|Ligation/Cloning and Transformation]] [[Media:JHU_0708_Colony_Screening_PCR.pdf|Moodle Colony Screening PCR]] [[Media:JHU_0708_Plasmid_Miniprep_Protocol.pdf|Plasmid Miniprep]] [[Media:CMBD.pdf|Checking Minipreps to Prepare for Sequencing]] [[Media:SSS.pdf|Sequence Sample Submission]] [[Media:JHU_Glycerol_Stocks.pdf|Making Glycerol Stocks]] [[Media:JHU_Moving_Insert_to_iGEM_Vector.pdf| Moving Insert to iGEM Vector]]

Important Papers
Reshma P Shetty, Drew Endy, and Thomas F Knight Jr; Engineering BioBrick vectors from BioBrick parts

[[Media:JHU_0708_paper_Aregularoryheirarchy.pdf| Herskowitz I.  A regulatory hierarchy for cell specialization in yeast.  (REVIEW.) Nature. 342:749-757 (1989) ]]

[[Media:JHU_0708_paper_MolecularMechanismofcell-typedeterminationinbuddingyeast.pdf|Johnson AD.  Molecular mechanisms of cell-type determination in budding yeast. (REVIEW.) Current Opinion in Gen. & Dev. 5:552-558 (1995) ]] [[Media:JHU_0708_paper_The_N-end_Rule_Pathway_as_a_Nitric_Oxide_Sensor_Controlling_the_Levels_of_Multiple_Regulators.pdf| Hu R-G et al. The N-end rule pathway as a nitric oxide sensor controlling the levels of multiple regulators. Nature. 437:981-986 (2005) ]]

[[Media:JHU_0708_paper_TheN-endRule.pdf|Varshavsky A. The N-end Rule pathway of protein degradation. (REVIEW.) Genes to Cells. 2:13-28 (1997) ]]

[[Media:JHU_0708_paper_Optimizedcassettes.pdf| Sheff MA & Thorn KS. Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast. 21:661-670 (2004) ]]

[[Media:JHU_0708_paper_Awalk.pdf| Bardwell L. A walk-through of the yeast mating pheromone response pathway. (REVIEW.) Peptides. 25:1465-1476 (2004) ]]

Yeast Vector Papers
[[Media:JHU_0708_paper_Development_of_a_hybrid_cloning_vector_and_isolation_of_the_can1_gene.pdf|Broach JR. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene. 8(1):121-133 (1979) ]]

[[Media:JHU_0708_paper_Manipulating_Yeast_Genome_Using_Plasmid_Vectors.pdf|Stearns, T. Manipulating yeast genome using plasmid vectors. (REVIEW.)</I> Methods in enzymology. 185:280-297 (1990) ]]

[[Media:JHU_0708_paper_Shuttle_Vectors_with_Multiple_Unique_Restriction_Sites.pdf| Hill JE et al.</I> Yeast/</I>E. coli Shuttle Vectors with Multiple Unique Restriction Sites.</I> Yeast. 2:163-167 (1986) ]]

[[Media:JHU_0708_paper_System_of_Shuttle_Vectors_and_Yeast_Host_Strains_Designed_for_Efficient.pdf|Sikorski RS. A System of Shuttle Vectors and Yeast Host Strains Designed for Efficient Manipulation of DNA in </I>Saccharomyces cerevisiae. Genetics. 122:19-27 (1989) ]]

[[Media:JHU_0708_paper_Yeast_vectors_for_the_controlled_expression_of_heterologous_proteins.pdf| Mumberg D et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds.</I> Gene. 156:119-122 (1995) ]]

Other
[[Media:JHU_0708_paper_TransfofYeast.pdf|JHU_0708_paper_TransfofYeast.pdf]] [[Media:JHU_0708_paper_Three-prongedgenomicanalysis.pdf|JHU_0708_paper_Three-prongedgenomicanalysis.pdf]] [[Media:JHU_0708_paper_SCPD_a_promoter_database_of_the_yeast.pdf|JHU_0708_paper_SCPD_a_promoter_database_of_the_yeast.pdf]] [[Media:JHU_0708_paper_Human_Left-Handed_Z-DNA_-_Schwartz_-_Science.pdf|JHU_0708_paper_Human_Left-Handed_Z-DNA_-_Schwartz_-_Science.pdf]] [[Media:JHU_0708_paper_GlobalTranscriptionRegulators.pdf|JHU_0708_paper_GlobalTranscriptionRegulators.pdf]] [[Media:JHU_0708_paper_ForeignGeneExpression.pdf|JHU_0708_paper_ForeignGeneExpression.pdf]] [[Media:JHU_0708_paper_DNAbendingnegativeregulatoryproteins.pdf|JHU_0708_paper_DNAbendingnegativeregulatoryproteins.pdf]] [[Media:JHU_0708_paper_BentDNA.pdf|JHU_0708_paper_BentDNA.pdf]] [[Media:JHU_0708_paper_Aregularoryheirarchy.pdf|JHU_0708_paper_Aregularoryheirarchy.pdf]]