Edinburgh/27 July 2008


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Sunday 27 July 08

 * Result of transformations: no growth for L19 (pSB1A2+rbs+dxs), L20 (pSB1A2+rbs+appY) or L21 (pSB1A2+rbs+crtB). For L22 (pSB1A2+rbs+crtI) there are some blue and white colonies. For L23 (pSB1A2+PzntA) there are many blue colonies but only a few white ones, suggesting that the SacI/SpeI double digest is less efficient than the XbaI/PstI which was (I hope) used for the others. For L24 (pSB1A2+crtE) there are many white colonies and very few blue - this is as expected, since in this case the vector and insert bands were purified from a gel. (CF)


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