Model

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  Figure Three, the concentraiton of antibiotic resistant protiens against time and its degration rate. Assume that the initial concentration of the two antibiotics (kanamycin and  chloramphenicol) are the same, and the amount of the antibiotic resistant  proteins comsumed per minute is [s]. When [s] is below 0.5uM, the expressions of the two antibiotic resistant genes will be enhanced when AHL or BHL is added  into the culture, and enter to the muatualism phase where the survival of one  strain is beneficial for the other. However, when [s] passes the threshold of 0.5μM, the extinction of one strain may happen before the two enter the  muatualism stage.  a        Figure Four, the  concentration of cells against time.  As is shown in the figure from the starting point to the third hour the gene circle  starts to work and finally get to a stable stage. And during this process there is some amount of chloramphenicol resistant protein and the kanamycin resistant  protein accumulated. However the expression of the antibiotic resistant protein has not reached the highest level. The third hour is the critical point for the AHL for the concentration of AHL will  get down to the same level as the BHL within 24 minutes and keep on this  cooperation. When the plux and prhl promoters have been activate to their greatest capacity there will be a small peak because of the leakiness of the  PBad/araC promoter. And in this situation the expression of the antibiotic resistant protein get to the highest level. By adding arabinose or IPTG at the eighth hour there still will be a small peak. However both of them will fall until the restarting of the PBad/araC promoter. From the starting point to the twelfth hour the whole mechanism can be seen as a  completely regulating process which shows the shifting from the competent state  to the mutualism state. The robustness of the whole system depend on several key factors listed below:        <li class="STYLE1"> <p class="STYLE9">The different inherent behaviors of the promoters determines the performance of the toggle switch. </li> <li class="STYLE1"> The degradation rates of the AHL（BHL）and arabinose(IPTG) will have some impact  in the next control. </li> </ul> </ol> <td height="20" colspan="2" valign="top" bgcolor="#0099FF"> <td colspan="2" valign="top" bgcolor="#03438A"><p align="center" class="STYLE9"> Figure Five, The expression of kanamycin resistant genes against time and Hill coefficient(Xm)  of the promoter Plux and Prhi. <p class="STYLE9">As is shown, the graph takes a look of Figure 1 only when Xm is within the range of 2.6-2.84. <p class="STYLE9">The result of this model, indicates that the capacity of promoters affect the viability of the system to a great extent, thus provide a powerful tool for the optimization of the design in terms of the choice promoters. <p class="STYLE9"> The assumptions of the model 1 The concentration of the signal molecules is even in all regions of the culture,  as well as within the cells. 2 The amounts of the antibiotic resistant proteins are enough to make sure the cells’  survival. <p class="STYLE9">3 The amount of antibiotics resistant proteins consumed per minute [s] is a constant when the  concentration of antibiotics is stable, and will vary with the variance of the  concentration of antibiotics. A strain is assumed to be extinguished when the amount of antibiotic resistant protein expressed is negative. <p class="STYLE9"> <p class="STYLE9">4 The inducers IPTG or arobinose will function for long, and their concentration  won’t be affected significantly when they combine the repressors. <p class="STYLE9">5 The survival ability of a cell is demonstrated by the amount of antibiotic resistant  proteins expressed. <td height="60" colspan="2" valign="top" bgcolor="#03438A"> P back to <a href="http://2008.igem.org/Project">Project</a> or <a href="http://2008.igem.org/Experiment">Experiment</a>