Illinois/19 July 2008

LAB WORK

Receptor Tyrosine Kinase method

glycerol added to 10%, cells in freezer

Transformation:

1. cells put in ice 30 min with 1μL plasmid(100ng)

2. Heat Shock for 2 minutes at 42 degrees

3. ice for 8 minutes

4. add cells to 1μL LB

5. grow for 1 hour

6. Plate 100 to 200 μL on LB and amp, grow overnight