TUDelft/28 August 2008

=28th August=

Double restriction
A double restriction was performed on the isolated plasmids to check insert size. It was done with EcoRI and SpeI from NEB, in a EcoRI buffer, using the protocol of iGEM-MIT. The result is in the gel below.

It can be seen in our gel that pSB1A3 and pSB1A7 have an insert of the wrong size. The other plasmids look fine.

Living stabs
We received our new requested living stabs from iGEM HQ. We put them in 5 ml LB + Amp. The parts received are:
 * R0080
 * B0034
 * B0032
 * B0030
 * I742161
 * B0015
 * B0021
 * B1006
 * F2620