Team:Chiba/protocol/gelcheck

>Protocol

Agarose gel electrophoresis


 * Agalose Gel casting
 * 1) Measure out the appropriate mass of agarose into glass bottle with the appropriate volume of TAE buffer
 * 2) Microwave until the agarose is fully melted
 * 3) Pour the agarose solution into the gelbox and let it cool for about 30 minutes, until the gel is solid
 * 4) Remove comb


 * Running agalose gel


 * 1) Load 5 μL prepared 1kbp ladder
 * 2) Mix DNA solution with loading dye(6x) and water
 * 3) Load it into agalose gel
 * 4) Run the gel at ~100 volts for 35 mins.


 * Visualizing agarose gels
 * 1) Remove gel from gel box
 * 2) Soak the gel in ethidium bromide solution
 * 3) Let it 30 min.
 * 4) Place the gel in Trans-Illuminator and turn on UV light after make sure the door closing.
 * 5) Print the picture.
 * 6) Remove gel and throw in trash
 * 7) Wipe down Trans-Illuminator if necessary.