Team:Warsaw/Calendar-Main/27 August 2008

Tests for ampicillin resistance given by protein added to medium Piotr Results of growth from previous day

Cloning of protein A DNA to pET15b+OmpA-alpha plasmid Michał K.

 Digest of pGeneart+A and pET15b+OmpA-alpha with NdeI and NotI (Orange buffer), pET15b was also  dephosphorylated. 

 Gel electrophoresis of digested plasmids (Fig. 1.) and gel-out</a> of proper bands (400 bp for A lane and 6200 bp for pET15b lane). </li>  Ligation</a> of pET15b-Alpha and A</a>. </li>  Transformation of E. coli TOP10</a>. </li>  Transformants plating on LB + ampicillin.</li>

<img src=" http://2008.igem.org/wiki/images/1/1b/Go29.jpg" width=300/></a> Fig. 1. NdeI/NotI digest of plasmid pGeneart+A 1. Marker 2. Digested plasmid pGeneart+A