TUDelft/14 August 2008

=August 14th 2008=

Today we started a set of new transformation with various slight differences in order to see if it would help in transformation efficiency. We tried solving the punches from the registry in either: - old TE - fresh TE - H2O

And with these we either: - added 4 ul to 50 ul competent cells - added 50 ul competent cells to all the DNA solution including the punch

As positive controls we transformed with pUC18 in H2O or in old TE with a blanc paper punch added, both incubated with the other samples at 42ºC for 20'.