Team:Hawaii/Notebook/2008-09-24

= Things we did today =

Verified plasmid preps

 * Grace


 * PCR of plasmid preps from yesterday to verify inserts
 * Ran PCR products on gel
 * nrg #1 and nrsg #2 returned blanks. Bad plasmid prep?
 * BB-pRL1383a and colony PCR of BB-pRL1383a E. coli both blank. Yikes!
 * Quantified BB-pRL1383a and pSB1A3 plasmid preps
 * Blanks. Really low conc.? Will nanodrop to check.

Construction of secretion device (cont.)

 * Grace


 * Overnight RE digest:
 * nir, nrsg, and nrg PCR products with EcoRI and SpeI
 * rgt PCR products with XbaI and PstI
 * J33207 PCR product and pRL1383a plasmid with HindIII and BamHI

Prep for sequencing

 * Grace


 * nrg #4, nrg #7, nrsg #8, rgt #1, rgt #2
 * plac+rbs
 * PCR machine froze while ExoSAPing. Unable to retrieve samples from machine. Jamie says she will figure it out.

Making Emergency BB Vector Insert (help contaminant troubleshoot)

 * Norman
 * Cross posted in 2008-09-23 for comparison with contaminated PCR gel run.

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"