Team:Warsaw/Calendar-Main/15 May 2008

Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7 Michał K.   PCR - AID for translational fusion.

Primers:

AIDlNrH AIDpLinB

Template DNA: pTrc99A-AID Annealing temperature: 55&deg;C

Elongation time: 60 s

20 cycles 

 PCR</a> - T7 RNA-polymerase for translational fusion; Taq polymerase with supplemented buffer (Łukasz Banasiak helped us with that problematic PCR).

Primers:

T7lLinkB</a> T7pXbSal</a>

Template DNA: E. coli Rosetta</a> genomic DNA

Annealing temperature: 62&deg;C

Elongation time: 4 minutes

20 cycles </li>

 PCR</a> - T7 RNA-polymerase for transcriptional fusion.

Primers:

T7lRBSHi</a> T7pXbSal</a>

Template DNA: E. coli Rosetta</a> genomic DNA

Annealing temperature: 62&deg;C

Elongation time: 4 minutes

20 cycles </li>