Melbourne/5 August 2008

Done today:

 * Red light retest.

4 transformations:
 * Cph8 + pPL-PCB
 * Cph8
 * PCB
 * -ve control

Protocol:
 * 2μL DNA
 * RU1012
 * 30 min ice
 * 1 min 42°C
 * 1 hour at 37°C + LB

Each transformation plated onto 2 plates, one of which is wrapped in foil. Placed in 37°C room at genetics which light on

Plates: Amp Kan Chlor Arabinose (2mM) IPTG (1mM) X-gal