Team:University of Ottawa/26 June 2008

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 Home  Welcome  The Team</a>  Who We Are</a>  Advisors</a></li> Undergrads</a></li> </ul> </li> What We've Done</a></li> Where We're From</a></li> Contact Us</a></li> </ul> </li> The Project</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Project_Overview">Overview</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#The_Template">Template</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#The_Design">Design</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Applications">Application</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#References">References</a></li> </ul> </li> <li><a href="http://partsregistry.org/Part:BBa_K149001:Design">BioBricks</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Modeling">Modeling</a></li> <li><a class="MenuBarItemSubmenu" href="http://2008.igem.org/Team:University_of_Ottawa/Wet_Lab">Wet Lab</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Lab_Protocols">Lab Protocols</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/WetWare">WetWare</a></li> </ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Notebook">Notebook</a></li> <li><a class="MenuBarItemSubmenu" href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors">Sponsors</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Acedemic_Sponsors">Academic</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Research_Sponsors">Research</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Corporate_Sponsors">Corporate</a></li> </ul> </li>

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Dan
 * PCR gel
 * <li>The gel turned out well, S&D&T amplification products had some non specific binding, but there was enough separation to cut the good bands out and purify.
 * <li> After PCR cleanup (for 0&1 amplification products) and gel extraction and cleanup (for S&D&T plasmids) absorbtion was measured in order to determine the concentration of the PCR products.
 * <li> S&D&T amplification products showed low DNA concentration, however it is still within the usable range
 * Digestion
 * <li> 0 and 1 A and B PCR products were digested with ClaI so that they could be ligated in various combinations