Protocols PCR

Cocktail:

37ul H2O 5ul 10X PCR Buffer 1ul dNTP (10mM) 0.5ul Polymerase 0.5ul of each Primer (100uM) 1ul Template _____________ 50ul ThermoCycle Program 98*C.......30 sec _____________ 98*C.......10 sec 65*C.......30 sec 72*C.......(20 sec/kb {Min 20sec}) _____________ 72*C.......5 min 10*C........Forever

Alternate ThermoCycle Program  90*C.......30 sec _____________ 92*C.......10 sec 62*C.......30 sec 72*C.......(20 sec/kb {Min 20sec}) _____________ 72*C.......5 min 10*C........Forever

Alternate ThermoCycle Program  90*C.......30 sec _____________ 92*C.......10 sec 62*C.......60 sec 72*C.......(20 sec/kb {Min 20sec}) _____________ 72*C.......5 min 10*C........Forever

Protocols