Template:Team:UC Berkeley/Notebook/CDB construction

pBca1256-K112400
Biobricking of promoter for grpE protein PCR grpE-F and grpE-R on MG1655 	 (129 bp, EcoRI/BamHI) Sub into pBca1256	                 (EcoRI/BamHI 910+2472bp, L) Product is pBca1256-K112400 cb0001-grpE-F Forward Biobricking   cgataGAATTCatgAGATCTccgaggtccttgttgcgaag cb0002-grpE-R Reverse Biobricking   cgtagGGATCCcgcattcatctcgctaacttc

pBca1256-K112401
Biobricking of promoter for recA protein PCR recA-F and recA-R on MG1655 	 (317 bp, EcoR1/BamH1) Sub into pBca1256	                 (EcoR1/BamH1 910+2472bp, L) Product is pBca1256-K112401 cb0003-recA-F Forward Biobricking   cgataGAATTCatgAGATCTagagaagcctgtcggcaccg cb0004-recA-R Reverse Biobricking   cgtagGGATCCcccagtgctgccgccaacgc

pBca1256-K112402
Biobricking of promoter for fabA protein PCR fabA-F and fabA-R on MG1655 	 (285 bp, EcoR1/BamH1) Sub into pBca1256	                 (EcoR1/BamH1 910+2472bp, L) Product is pBca1256-K112402 cb0005-fabA-F Forward Biobricking  cgataGAATTCatgAGATCTggccattacgttggctgaac cb0006-fabA-R Reverse Biobricking  cgtagGGATCCgaagttaccacccgtttcgg

pBca1256-K112403
Biobricking of signal peptide (prepro) of phoA (alkaline phosphatase) PCR cb10007-F and cb10008 on MG1655	(114 bp, EcoRI/BamHI) Sub into pBca1256	       	(EcoRI/BamHI 910+2472bp, L) Product is pBca1256-K112403 cb10007-phoA-F	cgataGAATTCatgAGATCTgtacatggagaaaataaaatgaaacaaagc cb10008-phoA-R	cgtagGGATCCgggcttttgtcacaggggtaaac

pBca1256-K112404
Biobricking of signal peptide (prepro) of lamB protein PCR cb10009-F and cb10010 on MG1655	(132 bp, EcoRI/BamHI) Sub into pBca1256	       	(EcoRI/BamHI 910+2472bp, L) Product is pBca1256-K112404 cb10009-lamB-F 		cgataGAATTCatgAGATCTgaaaagcaatgactcaggagatag cb10010-lamB-R 		cgtagGGATCCcagccattgcctgagcagacattac

pBca1256-K112405
Biobricking of promoter for cadAB genes PCR cad-F and cad-R on MG1655 	         (402 bp, EcoRI/BamHI) Sub into pBca1256	                 (EcoRI/BamHI 910+2472bp, L) Product is pBca1256-K112405 cb10011-cad-F          cgataGAATTCatgAGATCTggtatattccagacttctgttcc cb10012-cad-R          cgtagGGATCCtgaccagaccaaactggcg

pBca1256-K112406
Biobricking of cadC promoter and cadC gene PCR cadC-F and cadC-R on MG1655 	  (2336 bp, EcoR1/BamH1) Sub into pBca1256	                 (EcoR1/BamH1 910+2472bp, L) Product is pBca1256-K112406 cb10013-cadC-F                cgataGAATTCatgAGATCTcaccagcaatttgcgggcatatc cb10012-cad-R                cgtagGGATCCtgaccagaccaaactggcg

pBca1256-K112407
Biobricking of ygeF pseudogene promoter PCR ygeF-F and ygeF-R on MG1655 	  (540 bp, EcoR1/BamH1) Sub into pBca1256	                 (EcoR1/BamH1 910+2472bp, L) Product is pBca1256-K112407 cb10014-ygeF-F     cgataGAATTCatgAGATCTcagtttactcagtgaatcaatagagg cb10015-ygeF-R     cgtagGGATCCatttgattagagcaaaatccCaattcgcagc

pBjh1601CA-K112408
T4 derived lysis device Sub into pBjh1601KA		   (5025 bp, EcoR1/BamH1) Product is pBjh1601KA-K112408     (EcoR1/BamH1 3196/1824 S)