Alberta NINT/20 August 2008

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lab work (SD): Digested TA2+(PCR) with BamHI+NcoI. Incubated at 37 C for 1 hr.            Ran TA2+(PCR) (10ul) and TA2+(PCR)/B+N (10ul) on 2% agarose gel 110 V 60 min Used nucleotide removal purification kit to purify 10ul of TA2+(PCR)/B+N. 4.4 ng/ul 1.96 A260/280 67 bp = 99.499 fmol/ul The gel did not have a band for the 67 bp TA2+(PCR)/B+N. This may be because the concentration is             too low.

lab work (JD): Miniprep of O/N of cotransformed cells Day 2 of LacZ Assay