Team:Chiba/Calendar-Home/11 October 2008

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10 October 2008 <|> 12 October 2008

Team:Demo-Rs
~10 October
 * 1) Sender Wash
 * 2) Centrifuged 2 tubes containing(BBa_T9002(JW1908))at 20&deg;C,3600rpm for 6min and discarded supernatant.
 * 3) Added 10mL LB-Amp to each tube.
 * 4) Repeated wash twice.
 * 5) Creating bacterial plates
 * 6) LB-Amp pre-cultured Sender(BBa_S03623(JW1908)) tube(10mL) was mixed with LB-Amp-agar(50&deg;C)(10ml)to produce sender containing bacterialplate.
 * 7) Lifted with nitrocellulose
 * 8) Receiver(BBa_T9002(JW1908))colony was transfered to a nitrocellulose filter and placed on each of Sender(BBa_S03623(JW1908))containing bacterial plate and Sender-absent negative control plate (t=0).
 * 9) Method to detect fluorescence
 * 10) Plates cultured at 37&deg;C were exposed to UV (312nm) light once every 30 minutes to observe GFP fluorescence.