Team:Chiba/jk/β/week 5

>送受信班

>Protocols

14 September, 2008

 * (13/9)--->Mini prep


 * Transformation
 * Competent cells : XL10G
 * BBa_T9002(2007)
 * Plac+RhlI+LVA
 * Plac+CinI+LVA


 * --->(15/9)

15 September, 2008

 * Transformation
 * competent cells : JW1908
 * BBa_S03623
 * BBa_K084007
 * BBa_K084008
 * BBa_K084009
 * BBa_T9002

(15/9)-->T9002 check(AHL sensitivity and time response test)

16 September, 2008
(15/9)->
 * time-delay test
 * conditions
 * 1) strain Senders:JW1908 Receiver:JW1908
 * 2) temparature 37℃ and 30℃

17 September, 2008

 * time-delay test
 * conditions
 * 1) strain Senders:JW1908 Receiver:JW1908
 * 2) temparature 37℃ and 30℃


 * time-delay test
 * conditions
 * 1) strain Senders:XL10GOLD Receiver:JW1908
 * 2) temparature 37℃ and 30℃

18 September, 2008

 * time-delay test
 * conditions
 * 1) strain Senders:XL10GOLD Receiver:JW1908
 * 2) temparature 37℃ and 30℃

19 September, 2008
-->(?/9) Gel Check

--->Gel Extract (各サンプル well３つずつ)
 * note:ADB buffer添加後,37℃,6h
 * --->Zymo Clean


 * elution
 * J0450-->27
 * C0161-->11μL

--->Gel Check


 * --->Ligation


 * --->Transformation above ligation onto a LB-amp plate.


 * note
 * competent cell:XL10GOLD
 * DNA:1μL,SOC:120μL

--->(20/9)'''Mini prep


 * time-delay test
 * conditions
 * 1) strain Senders:XL10GOLD Receiver:JW1908
 * 2) temparature 30℃ and Room Temparature

20 September, 2008
--->(19/9)'''Mini prep

--->Gel Check
 * From left;
 * -> OK
 * -> OK
 * -> OK
 * -> OK


 * --->Digestion Test


 * --->Gel Check
 * From left;
 * -> OK
 * -> OK
 * -> OK
 * -> OK
 * -> OK
 * -> OK
 * -> OK
 * -> OK
 * -> OK

--->(?/9)Transformation

--->(22/9)'''phenotype check