Team:Michigan/Project/LandingPads

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= BioBrick Landing Pads  =

We will be using landing pads to insert our clock onto the chromosome of E. coli. Noisy behavior has proven detrimental to clock studies throughout the past, and we hope to reduce the noise in our system using landing pads.

Landing Pads used for the Sequestilator
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Operon
Activator Operon

Repressor Operon

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Landing Pad
Leucine Landing Pad constructed by former Ninfa lab member, Don Eun Chang

Arabinose Landing Pad iGEM 2007 project by Alyssa Delke & Khalid Miri


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2nd Operon in Arabinose Landing Pad
This is what our repressor operon would look like in our Arabinose BioBrick landing pad.

Notice that the landing pad plasmids are Ampicilin resistant, to allow for additional screeining. There is also a ScaI restriction site located in the AmpR gene, that is found nowhere else on the plasmid, that can be used for linearizing the plasmid. It is necessary to linearize the plasmid in order to cross it onto the chromosome.

For testing and results on these landing pads, please visit last year's iGEM page on Landing Pads HERE.

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Learn more about Landing Pads [http://synthbio.engin.umich.edu/wiki/index.php/BioBrick_Landing_Pad Here! ]


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