Team:Warsaw/Calendar-Main/4 July 2008

Change of the reporter from pZC320 with B-galactosidase to GFP or RFP Piotr, Weronika, Emilia  Isolation of plasmids from transformants. Control  digest of plasmids with NotI. Gel electrophoresis of digested DNA. There where proper clones, but they weren't red or green in UV (the reason might be low copy number of the plasmid or something else).

 No sucess in changing of the reporter from pZC320</a> with B-galactosidase to GFP or RFP.

Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega Paweł   <a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of plasmids from bacterial cultures inoculated on previous day (<a href=http://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA_alpha</a> and <a href=http://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-omega>pET15b+OmpA_omega</a>). </li>  Control <a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of plasmids with SacI and BamHI (BamHI buffer). </li>  Gel electrophoresis. </li> </ol>