Team:Hawaii/Notebook/2008-09-28

= Things we did today =

Ligation

 * Margaret


 * Ligation of promoter/rbs to rep into pSB1A3 (dephosphorylated)
 * Ligation of pSB1A3 (dephosphorylated) to self

Transformation into DH5-alpha

 * Margaret


 * promoter/rbs to rep into pSB1A3 (dephosphorylated)
 * pSB1A3 (dephosphorylated) to self ligation
 * (+) pUC18
 * (-) no plasmid

Culture DH5-alpha with plamids

 * Margaret


 * aadA (BB)with promoter/rbs/terminator
 * aadA (pRL)with promoter/rbs/terminator
 * rep
 * oriV
 * oriT (J33207)
 * oriTr (J33207)

Name of Task

 * Margaret


 * The sequence of rep (submitted 9/24) was verified using Seqman software
 * region between 1550-1600 was not covered by primers, so an additional primer was designed and ordered. Will submit again for sequencing

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"