Team:University of Ottawa/18 June 2008

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 Home  Welcome  The Team</a>  Who We Are</a>  Advisors</a></li> Undergrads</a></li> </ul> </li> What We've Done</a></li> Where We're From</a></li> Contact Us</a></li> </ul> </li> The Project</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Project_Overview">Overview</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#The_Template">Template</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#The_Design">Design</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Applications">Application</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#References">References</a></li> </ul> </li> <li><a href="http://partsregistry.org/Part:BBa_K149001:Design">BioBricks</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Modeling">Modeling</a></li> <li><a class="MenuBarItemSubmenu" href="http://2008.igem.org/Team:University_of_Ottawa/Wet_Lab">Wet Lab</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Lab_Protocols">Lab Protocols</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/WetWare">WetWare</a></li> </ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Notebook">Notebook</a></li> <li><a class="MenuBarItemSubmenu" href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors">Sponsors</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Acedemic_Sponsors">Academic</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Research_Sponsors">Research</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Corporate_Sponsors">Corporate</a></li> </ul> </li>

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Today in the lab
Dan and Matt
 * Performed digestion of Tet plasmids
 * <li> T123, D12, and S1 were all digested with NcoI and EcoRV, results appeared to be as expected however EcoRI should have been used instead of EcoRV in order to obtain a better resolution
 * Innoculated BY4741
 * <li>BY4741 was innoculated in 5 mL of 2xYPD + 1/10 20% glucose. However the BY4741 strain was 2 months old.

Chris
 * <li>Too few colonies grew on incubated plates; linear AtCRE may not have ligated properly
 * '''Comparison of Ligated and Unligated Samples
 * <li>To determine of recircularisation worked, the ligated and unligated samples of AtCRE were run on a 0.8% gel for about 50 minutes. A difference was expected between the two samples when visualised.
 * <li>Neither sample turned up on the gel; concentrations of both were too low. This prompted a re-examination of AtCRE concentrations by absorbance.
 * <li>The concentrations of both samples were incredibly low; ligation did not work.
 * Starting Over
 * <li>Digested AtCRE: 37 uL DNA, 1 uL EagI, 5 uL Buffer 3, 7 uL water.