Waterloo/21 August 2008

08/08/21 Thurs

[x] gel of full digestion digestion - not left long enough, will repeat - TT did not work, will reinoculate [] gel extraction [] ligation [] transformation

[x] inoculate TT, RBS X4 [] upload inventory / purchase list [] transform and make glycerol stocks of lasI" (29/5), PlacluxR (12/6), PlaclasI" (20/6), TTr B0014 (4/7), rfp" (17/7), gfp", T7 gene 6 27 and 29 (12/8) [] transform and test Pconst rfp' -1 and -2, Plac rfp"-3 [x] Check with diagnostic gel: PlacI (16/7), PocI (11/7) and more

[x] prepare 5mL culture tubes [x] Stuff tips and autoclave