Team:University of Ottawa/30 July 2008

  Untitled Document  

 Home  Welcome Announcements</li> </ul> The Team</a> <ul> Who We Are</a> <ul> Advisors</a></li> Undergrads</a></li> </ul> </li> What We've Done</a></li> Where We're From</a></li> Contact Us</a></li> </ul> </li> The Project</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Project_Overview">Overview</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Pulsate_Gene_Expression">Expression</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Cell-to-Cell_Communication">Communication</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Oscillatory_Dynamics">Oscillation</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Applications">Application</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Journal_Club">Journal Club</a></li>

</ul> </li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Parts">BioBricks</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Modeling">Modeling</a></li> <li><a class="MenuBarItemSubmenu" href="http://2008.igem.org/Team:University_of_Ottawa/Wet_Lab">Wet Lab</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Lab_Protocols">Lab Protocols</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/WetWare">WetWare</a></li> </ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Notebook">Notebook</a></li> <li><a class="MenuBarItemSubmenu" href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors">Sponsors</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Acedemic_Sponsors">Academic</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Research_Sponsors">Research</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Corporate_Sponsors">Corporate</a></li> </ul> </li>

</ul> <script type="text/javascript">

=Today in the Lab= Tammy


 * <li> Digestion Step 1 of pSSA14 with PstI


 * <li> Digestion Step 1 of OB with PstI


 * <li>Digestion Step 2 of pSSA14 with ClaI


 * <li>Digestion Step 2 of OB with ClaI

Chris
PCR Confirmation
 * <li> Ran all 5 PCR product samples on a 1% gel for 40 minutes at 80 V
 * <li> Desired band appeared on gel; PCR amplification was successful.
 * <li> Used PCR cleanup kit to purify samples
 * <li> Measured absorbance of PTP2 samples

Digestion of PTP2
 * <li> Digested PTP2 with BamHI and XhoI; ran 5 tubes in parallel. Incubated at 37 C for on hour. Ran one water control.
 * <li> Used PCR cleanup kit to purify digestion products. Measured absorbance to determine concentration.
 * <li> Concentrations too low for ligation.

Matt

 * <li> Measured absorbance of pSSA42 gel extraction products from digestion.

Dan
Gel Extraction
 * <li>T123 amplification product was run on a gel and extracted.
 * <li>24 gel extraction were performed in total, care was taken to minimize UV exposure (samples were not exposed for longer than 20s)

Gel of extracted PCR amplification product
 * <li> Expected bands were seen, the T123 is good to go.

0A and 0B absorbances
 * <li> absorbances of 0A and 0B indicated very good concentrations

Ligation and digestion
 * <li> Digestion (with SphI and PstI) of T123 0A and 0B were all performed and left overnight