Newcastle University Wetlab/10 September 2008

Wednesday 10th September
- 12.5μl pGFPrrnB-BBa_746107 + 100μl DH5α cells - 12.5μl pGFPrrnB + 100μl DH5α cells (positive control) - 100μl DH5α cells (negative control)
 * Overnight ligation mixtures transformed into competent DH5α E. coli cells (see protocol).


 * All transformants were then plated onto spectinomycin agar in both 25μl and 250μl volumes and incubated overnight at 37˚C.