Team:University of Chicago/Notebook/Glycerol stocks

Materials

 * 40% glycerol solution
 * Cryogenic vials

Method

 * 1) Add 1 ml of 40% glycerol in H2O to a cryogenic vial.
 * 2) Add 1 ml sample from the culture of bacteria to be stored.
 * 3) Gently vortex the cryogenic vial to ensure the culture and glycerol is well-mixed.
 * 4) * Alternatively, pipet to mix.
 * 5) Use a tough spot to put the name of the strain or some useful identifier on the top of the vial.
 * 6) On the side of the vial list all relevant information - part, vector, strain, date, researcher, etc.
 * 7) Store in a freezer box in a -80C freezer. Remember to record where the vial is stored for fast retrieval later.