Team:Hawaii/Notebook/2008-06-17

= Things we did today =

Tested competency of new batch of competent cells

 * Grace


 * Same protocol as 6/3/08 competency test
 * Plated 20 &mu;l untransformed competent cells on LB+amp100 as negative control

Biobricks extraction

 * Grace


 * Extracted BBa_B0024, BBa_B0025, and BBa_E0240 from filter paper using same protocol 6/9/08

Alkaline lysis

 * Grace


 * Made stock solutions
 * 0.5M EDTA
 * 1M Tris-HCl (pH 8.0)
 * 5M KOAc (3M potassium, 5M acetate, pH 4.8)
 * Made alkaline lysis solutions
 * GTE (50 mM glucose, 25 mM Tris-HCl, 10 mM EDTA)
 * 0.2M NaOH/1% SDS
 * Extracted pRL1383a from transformed cells (see experiment)

Made TES buffer

 * Norman

Primers

 * Norman


 * Refined primers

= Discussion =
 * Plasmid has not dried yet (ethanol still evaporating) so it will dry in the hood overnight. Norman will resuspend the pellet in 30 &mu;l TE buffer tomorrow morning.

= Quote of the Day = "'You could have had a Nature publication, you know.' - Dr. Presting, in reference to a cytochrome article and Grace's refusal to work with the pigments"