Team:Chiba/jk/γ/week 6

>実験ログ >出力班

22,September,2008
Time delay

23,September,2008
Digestion test BBa_E0240

TRANSFORMATION BBa_I6107 BBa_I6206 BBa_I6101 BBa_I6042 BBa_I0462

Digestion Vector:BBa_R0062① Insert:BBa_E0240② Vector:BBa_I7100③ Insert:BBa_R0062④ Insert:BBa_J37033⑤

->1hour 37℃ ->SAP 1μl(Vector only) ->1hour 37℃ ->30min 65℃ ->Zymo ->10μl Gel cheack

->③&④&⑤のLigationはしない

24,September,2008
Ligation Vector:BBa_R0062① Insert:BBa_E0240②

->TRANSFORMATION

25,September,2008
colony PCR Vector:BBa_R0062 Insert:BBa_E0240 Vector:BBa_R0062

->95℃ 5min ->(95℃ 1min 50℃ 30sec　72℃ 1min)x25 ->72℃ 10min

Digestion Test BBa_I6206 BBa_I6101 BBa_I6042 BBa_I0462

->30min 37℃ Gel cheack BBa_I6206(digestion product) BBa_I6101(digestion product) BBa_I6042(digestion product) BBa_I0462(digestion product) BBa_I0642(natural)

Digestion BBa_I0462

->3hour 37℃ ->Zymo ->10μl

Ligation Vector:BBa_I7100 Insert:BBa_R0062 Insert:BBa_I0462

->3hour 37℃ ->Transformation

26,September,2008
TIME DELAY TEST

colony PCR Ligation product(26,September)x7 Vector:BBa_R0062

->95℃ 5min ->(95℃ 1min 50℃ 30sec　72℃ 1min)x25 ->72℃ 10min ->Gel cheack ->MINI Prep

27,September,2008
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