Team:ESBS-Strasbourg/Protocols

=Protocols= {|
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E.Coli

 * Preparation of competent cells (Barbara's Protocol)
 * Preparation of TOP10 chemically competent cells (iGEM)
 * Transformation of competent cells (Barbara's Protocol)
 * Heat shock transformation


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Cloning

 * PCR
 * Purification of PCR products
 * Miniprep:Plasmid Amplification
 * Plasmid Purification
 * Restriction Digestion
 * Agarose Gel Preparation
 * Dephosphorylation
 * Ligation
 * Concentrations Measurements
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Solutions and buffers

 * SOB for the preparation of competent cells
 * SOC for bacterial transformation purposes
 * TENS for the do it yourself MiniPrep
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Further source for protocols: Protocol-online.org