Team:Harvard/Shewie

table { background-color: #c4dbea; font-color: #333333; color:white; }

a.menu { background-color: #c4dbea; color: white; width: 12em; }

.firstHeading { color:white; }

background-color: #c4dbea; }
 * 1) bodyContent {

background-color: #c4dbea; }
 * 1) content {

background-color: #c4dbea; } p { color:#333333; } body { background-color:#c4dbea; } .firstHeading { display:none; }
 * 1) footer-box {

{| 
 * align="center" style="background:#c4dbea" border="0" cellpading="0" cellspacing="0"|

{|style="color:#1b2c8a;" cellpadding="0" cellspacing="0" border="0" bordercolor="#000" width="100%" align="center"|}

{|align="justify" style="background-color:#FFFFFF;text-indent: 15pt; text-align:justify" cellpadding="50" width="90%" =Organism= Most of our work this summer is founded upon the diverse metabolism of the bacterium Shewanella oneidensis MR-1. Throughout the summer, we came to better understand how to work with this organism, and we hope our findings will help establish S. oneidensis as a interesting chasis for synthetic biology.

So, who is this "Shewie"?
This summer we worked with Shewanella oneidensis MR-1, a gram-negative facultative anaerobe (Myers and Myers 1997). Under anaerobic conditions, it reduces a number of electron acceptors such as MN(IV). This ability can be harnessed by microbial fuel cells (MFC) to produce an electric current (Bretschger et al. 2007). When the bacteria are grown anaerobically in the anode chamber of an MFC, they release electrons onto the electrode, creating an electrical current. These diverse respiratory capabilities require a complex electron transport systems, including 39 c-type cytochromes (Heidelberg et al. 2002). These characteristics of S. oneidensis MR-1 make it an important organism for toxin-reduction based bioremediation and biotechnology applications.

Online resources for working with S. oneidensis
-| Codon usage table

-| Annotated genome, with information on RBS, terminators, protein domains, gene ontology, etc

-| In silico PCR of S. oneidensis genome

Chassis and the Registry
To facilitate easy manipulation in different organisms, it may be advantageous for the Registry to standardize a chassis specification sheet. Below, we provide a quick summary of S. oneidensis MR-1 following what we think may be a suitable documentation format. Since iGEM teams frequently work with species other than E. coli, if only to clone some interesting gene product, a set of such sheets could be built up to facilitate synthetic biology in a more diverse set of organisms. A PDF version with links is available.
 * }


 * }