Brown: Team Resistance/27 October 2008

October 27, 2008

 * Another miniprep of the S105 gene transformation performed.
 * Optical Density Measurement: 71.6 ng/ul
 * Restriction Digest Verification done of S105 gene with restriction enzymes EcoRI and PstI (Buffer H). Placed in thermocycler for 1 hour at 37 degrees and heat shock 20 minutes at 80 deg C.


 * Performed another ligation of PVJ4 and pSB1AK3.


 * We did see growth for the transformations of pVJ4 and S105 Cassette!! Cultures were placed in the incubator overnight for miniprepping tomorrow.


 * Optical Density and conductance test run again with little success. Solution is not clearing up as expected.  Cultures will be made from the glycerol stock plates (Colonies 2,3, and 4).