Team:Montreal/Notebook/July

July 1

 * Maxi-prep of J-brick, assuming high copy number plasmid.

July 2

 * Gel of J-brick maxi-prep, results indicate large plasmid at well over 13K. Assumed incorrect, to be repeated.

July 3

 * Repeat gel, of J-brick maxi prep from July 1st, negative results. Gel from July 2nd assumed contamination from other lanes.

July 4

 * Preparation of media.

July 7

 * Seeding of J-brick for mini-prep, 4x5mL cultures with ampicillin.

July 8

 * Mini-prep of J-brick cultures followed by gel giving negative results - no DNA seen, but ladder appears normal.