Team:Warsaw/Calendar-Main/24 June 2008

Preparation of alpha-A and omega-A fusions Michał L., Ewa, Marcin

Still no success. We need to run gradient PCR to find optimal reaction conditions.

Preparation of constructs: OmpA_alpha and OmpA_omega Michał K.  PCR on isolated plasmids with OmpaL_N and OmpaP_link primers.

Gel electrophoresis (Fig. 1, no proper clones found). 

 Fig. 1. PCR product - searching for fusions with OmpA protein. Lower lanes - OmpA_alpha, upper - OmpA_omega.