Team:Hawaii/Notebook/2008-10-28

= Things we did today =

Antibiotic test

 * Grace


 * Ran HindIII/BamHI digested J33207 on gel
 * Extracted from gel
 * Ligated with HindIII/BamHI digested pRL1383a
 * Used 5 &mu;l ligation reaction to transform 100 &mu;l DH5&alpha; MCR cells (from Doug in SC lab)
 * Also transformed 25 &mu;l DH5&alpha; MCR with 2 &mu;l BB-1 1025 and 1 &mu;l J33207 each.



Checked for Transformants

 * Krystle


 * BB-pRL1383a/K125820 produced a total of 27 transformants of varying size after incubation for 20 hours at 37oC

Inoculate for Tri-Parental Conjugation

 * Krystle


 * Inoculated 10ml cultures of LB with
 * BB-pRL1383a with sp100
 * BB-pRL1383a/K125820 with sp100
 * RP1 with kan50

Project page

 * Grace


 * Wrote up Materials, Methods and Results for Project Part B on team project page.

Sequence analysis

 * Grace


 * Same G->C transversion in CAP binding site for BBpRL-1 sequence.

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"