Team:University of Ottawa/18 July 2008

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Today in the Lab
Matt
 * PCR Amplification
 * <li> A PCR Amplification was performed on 600/1 plasmids with integration primers so that yeast integration can be performed next week. Corey suggested it is better to make new stock of this PCR amplification product for integration.
 * Transformation
 * <li> The transformation was a success with both plates streaked growing numerous colonies.
 * <li> For miniprep on Monday, I think I will choose 5 colonies per plate.
 * <li> An inoculation will be performed today into liquid media so that a miniprep can be performed.

Dan
 * PCR
 * <li> PCR amplification of 0B and 1 A was performed today, absorbance measurements indicated decent concentrations
 * Digestion
 * <li>1A 0B and T were pooled and digested with PstI and SphI as part of our new strategy for designing the constructs. It should help eliminate virtually all possible byproducts.
 * Ligation
 * <li> Was performed overnight.

Tammy
 * Gel Electrophoresis of pDR197::AtCKX2 Digestion Products
 * <li> Total plasmid size = 7.9 kb
 * <li> AtCKX2 insert size = 1.67 kb (NM_127508)
 * <li> pDR197 vector only = 6.23 kb
 * Glycerol Stock confirmed pDR197::AtCKX2
 * <li> Stocked PURE I and 1:10 II
 * <li> 0.5 mL Sample
 * <li> 0.5 ml LB + Glucose