Team:Edinburgh/Protocols

= Protocols =

As part of our project we developed and tested a number of procedures to make BioBrick production easier, more reliable and less hassle. These are the following:


 * Use of Edinbrick1 in preparation of BioBricksTM from PCR products
 * Site-directed mutagenesis using the MABEL protocol
 * BABEL: BioBrickTM assembly with blunt-ended ligation
 * Bacillobricks: introduction of BioBricks into Bacillus subtilis