Team:The University of Alberta/6 October 2008

David
i digested pUC57 pTET-BisdA with SphI+PstI+CIP treatment pTET+BisdB with XbaI+PstI

i gel purified the products and set up a ligation of pUC57-pTET-BisdA+pTET-BisdB overnight at 16 degrees C.