Edinburgh/18 September 2008

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Thursday 18 September ~ Friday 19 September 08

 * Sent M213, M218 (dxs+crtEBI) for sequencing. (AM)
 * Single (EcoRI) and double (EcoRI+PstI) digests of:
 * M198 (SOB2+SU1)
 * M201~203 (SOB2-rbs+SU1)
 * M204~206 (SOB2+ISO2)
 * M207~209 (SOB2-rbs+ISO2). (Yan)
 * Single (PstI) digest of P97 (cex mut). (AM)
 * M198~M206 run on Gel 80. All failed: single and double digests yielded the same results for all samples. (Yan)
 * M207~209 and P97 run on Gel 81. All failed: single and double digests yielded the same results for M207~209. PstI digest of P97 resulted in a smear. (AM)
 * Transformation/plating of CHU1727 failed twice. (CKL)

Follow-Up: Sequencing Results

 * M212 is pSB1A2-glgC16 with desired prefix and suffix.
 * M213 is NOT dxs+crtEBI. Both forward and reverse sequences indicate crtI.
 * M218 is NOT dxs+crtEBI. Forward sequence is dxs; reverse sequence is gibberish.