Team:ESBS-Strasbourg/25 July 2008

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WetLab
Manuel Paul -Digestion of tetR, lacI, c1, in order to check if the biobricks are really in the plasmid. Digestion with EcoR1 and Spe1
 * PCR VFprimer lacI BB
 * Gelpurification
 * 2nd PCR
 * on product of first PCR with original primer
 * original primer directly on BB plasmid
 * Miniprep of J6300X (x=2,3,5,8)
 * Verification gel of miniprep

-Digestion of the PCR products tetR, lexA, Gal4, in order to have them in a silver standard and to insert them into the vector p1010 1A2. In this way, this vector is also digested for future insertion of biobricks. Enzymes used: EcoR1 and Spe1.

General

 * Rechecked Primers to order (MM)