Team:Edinburgh/cex

= cex =

For better alignment see .txt file

AMINO ACID SEQUENCE
MPRTTPAPGHPARGARTAlRTTLAAAAATLVVGATVvlPAQAATTLKEAA

DGAGRDFGFALDPNRLSEAQYKAIADSEFNLVVAENAMKWDATEPSQNSF

SFGAGDRVASYAADTGKElYGHTLVWHSQlPDWAKNLNGSAFESAMVNHV

TKVADHFEGKVASWDVVNEAFADGGGRRQDSAFQQKLGNGYIETAFRAAR

AADPTAKlCINDYNVEGINAKSNSLYDLVKDFKARGVPLDCVGFQSHLIV

GQvPGDFRQNlQRFADlGvDvRITELDIRMRTPSDATKLATQAADYKKVv

QACMQvTRCQGvTVWGITDKYSWvPDVFPGEGAAlvWDASYAKKPAYAAv

MEAFGASPTPTPTTPTPTPTTPTPTPTSGPAGCQvlWGVNQWNTGFTANV

TvKNTSSAPVDGWTLTFSFPSGQQVTQAWSSTVTQSGSAvTVRNAPWNGS

IPAGGTAQFGFNGSHTGTNAAPTAFSLNGTPCTVG*

DNA SEQUENCE
atgcctaggaccacgcccgcacccggccacccggcccgcggcgcccgcac 50

tacggatcctggtgcgggcgtgggccggtgggccgggcgccgcgggcgtg

cgctctgcgcacgacgctcgccgccgcggcggcgacgctcgtcgtcggcg 100

gcgagacgcgtgctgcgagcggcggcgccgccgctgcgagcagcagccgc

ccacggtcgtgctgcccgcccaggccgcgaccacgctcaaggaggccgcc 150

ggtgccagcacgacgggcgggtccggcgctggtgcgagttcctccggcgg

gacggcgccggccgggacttcggcttcgcgctcgaccccaaccggctctc 200

ctgccgcggccggccctgaagccgaagcgcgagctggggttggccgagag

ggaggcgcagtacaaggcgatcgccgacagcgagttcaacctcgtcgtcg 250

cctccgcgtcatgttccgctagcggctgtcgctcaagttggagcagcagc

ccgagaacgcgatgaagtgggacgccaccgagccctcgcagaacagcttc 300

ggctcttgcgctacttcaccctgcggtggctcgggagcgtcttgtcgaag

tccttcggcgcgggcgaccgcgtcgcgagctacgccgccgacaccggcaa 350

aggaagccgcgcccgctggcgcagcgctcgatgcggcggctgtggccgtt

ggagctgtacggccacacgctcgtatggcactcgcagctgcccgactggg 400

cctcgacatgccggtgtgcgagcataccgtgagcgtcgacgggctgaccc

cgaagaacctcaacggctccgcgttcgagagcgcgatggtcaaccacgtg 450

gcttcttggagttgccgaggcgcaagctctcgcgctaccagttggtgcac

acgaaggtcgccgaccacttcgagggcaaggtcgcgtcgtgggacgtcgt 500

tgcttccagcggctggtgaagctcccgttccagcgcagcaccctgcagca

caacgaggcgttcgccgacggcggcggccgccggcaggactcggcgttcc 550

gttgctccgcaagcggctgccgccgccggcggccgtcctgagccgcaagg

agcagaagctcggcaacggctacatcgagaccgcgttccgggcggcacgt 600

tcgtcttcgagccgttgccgatgtagctctggcgcaaggcccgccgtgca

gcggcggacccgaccgccaagctgtgcatcaacgactacaacgtcgaggg 650

cgccgcctgggctggcggttcgacacgtagttgctgatgttgcagctccc

catcaacgcgaagagcaactcgctctacgacctcgtcaaggacttcaagg 700

gtagttgcgcttctcgttgagcgagatgctggagcagttcctgaagttcc

cgcgcggcgtcccgctcgactgcgtcgggttccagtcgcacctcatcgtc 750

gcgcgccgcagggcgagctgacgcagcccaaggtcagcgtggagtagcag

ggccaggtgccgggcgacttccggcagaacctgcagcggttcgcggacct 800

ccggtccacggcccgctgaaggccgtcttggacgtcgccaagcgcctgga

gggcgtggacgtgcgcatcaccgagctcgacatccgcatgcggacgccct 850

cccgcacctgcacgcgtagtggctcgagctgtaggcgtacgcctgcggga

ccgacgcgaccaagctcgcgacccaggcggccgactacaagaaggtcgtg 900

ggctgcgctggttcgagcgctgggtccgccggctgatgttcttccagcac

caggcctgcatgcaggtgacccgctgccagggcgtgaccgtctggggcat 950

gtccggacgtacgtccactgggcgacggtcccgcactggcagaccccgta

caccgacaagtactcgtgggtgccggacgtcttcccgggcgagggggccg 1000

gtggctgttcatgagcacccacggcctgcagaagggcccgctcccccggc

cgctggtgtgggacgcgagctacgccaagaagccggcctacgccgccgtg 1050

gcgaccacaccctgcgctcgatgcggttcttcggccggatgcggcggcac

atggaggccttcggcgcgagcccgacgccgacgcccaccacgccgacccc 1100

tacctccggaagccgcgctcgggctgcggctgcgggtggtgcggctgggg

gacgcccacgacgccgacgccgaccccgacgtccggtccggccgggtgcc 1150

ctgcgggtgctgcggctgcggctggggctgcaggccaggccggcccacgg

aggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtc 1200

tccacgacaccccgcagttggtcaccttgtggccgaagtggcgcttgcag

accgtgaagaacacgtcctccgctccggtcgacggctggacgctcacgtt 1250

tggcacttcttgtgcaggaggcgaggccagctgccgacctgcgagtgcaa

cagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtca 1300

gtcgaagggcaggccggtcgtccagtgggtccgcacctcgagctgccagt

cgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcg 1350

gcgtcaggccgagccggcactgccaggcgttgcggggcaccttgccgagc

atcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacggg 1400

tagggccgcccgccgtggcgcgtcaagccgaagttgccgagcgtgtgccc

caccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacgg 1450

gtggttgcggcgcggctgccgcaagagcgagttgccgtgcgggacgtgcc

tcggctga 1458

agccgact

Forbidden Restriction Sites: PstI x 1.

See below for mutagenic primers.

BIOBRICKTM PRIMERS
Forward: CGT t tctagATG CCT AGG ACC ACG CC

Reverse: CGT ctgcag cggccgc t actagt a TTA TTA GCC GAC CGT GCA GG

MUTAGENIC PRIMERS
781-786 PstI (Gln CAG --> CAA in forward primer)

Forward (786-799): A CGG TTC GCG GAC CTG G (58°C)

Reverse (785-768): TG CAG GTT CTG CCG GAA G (58°C)