Alberta NINT/11 July 2008

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lab work (SD): Measured cell count using spectrophotometer of 10/07/08 overnights. Lac Z Activity Assay was unsuccessful - perhaps not enough cells were used?

lab work (JD): Carried out Day 2 protocol for Activity Assay Like stated above, was not successful. Collected quantitative data of restriction fragments for ligation Ligated K102003+pSB4A5=K102031 and K102006+pSB4A5=K102021 Transformed XL1-B Cells Plated cells on LB + Amp50