Rensselaer/23 August 2008

Promoter Group

Aplification of J61002 mRFP plasmid

Pick 2 colonies to 2 15 ml tubes with 5 mls 2XYT and 75 ug/ml ampicillin.

Grow @ 37C ON w/ shaking. '''

Fe Promoter Ligation

Looked @ ligation plates.

Semiconfluent, but several colonies expressed mRFP.

Pick two red colonies to 5 mls 2XYT and 75 ug/ml ampicillin.

Grow ON @ 37C w/ shaking.