Team:Warsaw/Calendar-Main/15 October 2008

Preparation of linker_alpha (BBa_K103009) Michał K.

Inoculation of colonies from plate with ligation of pSB2K3 + linker_alpha (BBa_K103009).

Preparation of OmpA-linker-omega-linker (BBa_K103016) Piotr, Michał K.  Repetition of ligation of isolated DNA fragments: pACYC177 + OmpA-linker-omega-linker (BBa_K103016) (from 30 September</a>).</li> <li> Transformation</a> of TOP10</a> with above ligation.</li> <li> Plating on LB with kanamycin.</li></ol>

Preparation of AID under pBAD/araC (BBa_K103002)</a> Michał K. Inoculation of colonies from plate with ligation of pSB1A3</a> + AID under pBAD/araC (BBa_K103002)</a> to LB with ampicillin.

Preparation of OmpA_linker_alpha_linker under Plac (BBa_K103017)</a> Michał K. Inoculation of colonies from plate with ligation of pSB2K3</a> (with lactose promoter and OmpA_linker) + alpha2 to LB with kanamycin.

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