Team:The University of Alberta/21 October 2008

David
pUC57-pTET-BisdA with SphI+PstI with CIP treatment pTET-BisdB with XbaI+PstI laqIQ-ERE-TetR with XbaI+PstI
 * digested:

pUC57-pTET-BisdA+pTET-BisdB pSB1A3+laqIQ-ERE-TetR
 * set up the following ligations with the gel purified products:


 * overnight at 16 degrees C.

TOm

 * sequenced ETFA/B col5. with reverse universal

Benny

 * sequenced ETFA/B col5. with Vf and Vr
 * sequenced 21-25 col1. with Vf and Vr
 * digested 21-25, ETFA/B with X/P
 * run digest on a gel