Reference

1 A sensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds. Patricia Spiekermann · Bernd H. A. Rehm · Rainer Kalscheuer · Dirk Baumeister · Alexander Steinbüchel. Arch Microbiol (1999) 171:73–80

This literature explains how pBHR68 works and how PHA granules are detected.

2 Construction and Selection of  the  Novel  Recombinant Escherichia cob Strain  for Poly(Hydroxybutyrate)  Production.HUIMIN YU,JIN  YIN,HONGQI LI,SHENGLI   YANG,AND  ZHONGYAO. JOURNALOF BIOSCIENCEAND BIOENGWEERING Vol. 89, No. 4, 307-311. 2000

This literature indicates how PHA sysnthesis is realized in Escherichia coli.

3 Spontaneous liberation of intracellular polyhydroxybutyrate granules in Escherichia coli.Il Lae Jung, Ki Heon Phyo, Kug Chan Kim, Hyo Kook Park, In Gyu Kim.Research in Microbiology 156 (2005) 865–873

This literature introduced a method to release PHB granules from Escherichia coli.

4 Simultaneous Expression of Vitreoscilla Globin Gene and Lytic Genes of Phage lambda Novel RecombinantEscherichia Coli Used for Production of PHB.YU Huimin,SHI Yue,YIN Jin,and SHEN Zhongyao.ChineseJ.ofChem.Eug，(4)407一411(2001)

This literature introduced phage lambda lytic gene into Escherichia coli strains.

5 Autoregulator Protein PhaR for Biosynthesis of Polyhydroxybutyrate [P(3HB)] Possibly Has Two Separate Domains That Bind to the Target DNA and P(3HB): Functional Mapping of Amino Acid Residues Responsible for DNA Binding.Miwa Yamada,Koichi Yamashita,Akiko Wakuda,Kazuyoshi Ichimura,Akira Maehara,Michihisa Maeda,and Seiichi Taguchi.JOURNAL OF  BACTERIOLOGY, Feb. 2007, p. 1118–1127

This literature illustrates how PhaR works in the PHB synthesis regulation.

6 Influence of  homologous  phasins  (PhaP)  on PHA  accumulation  and  regulation  of  their expression  by  the  transcriptional  repressor PhaR  in  Ralstonia  eutropha  H16.Markus  Po¨tter,  Helena  Muller  and  Alexander  Steinbuchel.Microbiology  (2005),  151,  825–833.

This literature illustrates in the interaction of PhaP and PhaR.