Imperial College/24 September 2008

=24 September 2008=

Miniprep

 * 8 Minipreps were carried out for samples G7-1, G7-2, G8-1, G9-1, G9-3, G10-3, G11-2 and G11-3

Miniprep Digestion

 * All the mini-preps were digested with EcoRI and PstI




 * All digested bands appear to be approximately the right size, no trace of contamiantion beyond occaisonal bits of denatured DNA


 * Minipreps of PgsiB-gsiB, PgsiB-spoVG and Pveg-spoVG were digested with EcoRI and PstI and run on a gel along with uncut DNA from the minipreps of GeneArt constructs 4 and 5




 * The sizes of inserts for PgsiB-gsiB and PgsiB-spoVG appear correct and the mini-preps seem fairly pure. No insert was cut from the Pveg-spoVG minipreps and they will need to be re-ligated for transformation

Modelling Motility

 * Managed to run trajectory modelling on 90 cells yesterday.
 * Manual segmentation of runs done today to obtain a better curve fit. Hence certain cells will have two or more runs, and two or more parameter sets respectively.
 * Example is shown with 100908 Video 15 Cell 6. Notice that the model for a single run does not fit the data properly. Hence we introduce a change in orientation (parameters) at frame 75. This results in two separate runs, each with its respective set of parameters A, B and alpha.