GROUP 1: Fluorescent Proteins

GROUP 1: Fluorescent Proteins
Date: July 1 2008 status report by: Ingrid (work done by James) Part no.: BBa_K110017 Part Description: yESapphire Part Location (in build a genome lab): In James and Jasper's PCR product Box, Stainless Steel 4 degree PCR successful?; Yes Cloning of PCR product successful: Y/N Sequencing of cloned PCR product successful: Not done Joining of validated part to adjacent part(s) status: Not done Current status of this part: This part is being Sequenced

Date: July 1 2008 status report by: Ingrid (work done by James) Part no.: BBa_K110010 Part Description: yESapphire Part Location (in build a genome lab): In James and Jasper's PCR product Box, Stainless Steel 4 degree PCR successful?; Yes Cloning of PCR product successful: Y/N Sequencing of cloned PCR product successful: No Joining of validated part to adjacent part(s) status: Not done Current status of this part: This part is being sequenced

Date: July 17 2008 status report by: Ingrid Part no.: BBa_K110018 Part Description: mCherry: Part Location (in build a genome lab): In silver fridge by door PCR successful?; Yes (http://baderlab.bme.jhu.edu:8888/moodle18/mod/data/view.php?d=4&rid=1471) Cloning of PCR product successful: Inconclusive Venus YFP and mCherry Miniprep check via digest Sequencing of cloned PCR product successful: Not done Joining of validated part to adjacent part(s) status: Not done Problems to be solved: Had some extra unknown products, with unknown bands in the gel Current status of this part: Done with touchdown PCR and gel. Next step is to clone product.

Date: July 17 2008 status report by: Ingrid Part no.: BBa_K110019 Part Description: mCherry: Part Location (in build a genome lab): In silver fridge by door PCR successful?; Yes (http://baderlab.bme.jhu.edu:8888/moodle18/mod/data/view.php?d=4&rid=1471) Cloning of PCR product successful: Inconclusive Venus YFP and mCherry Miniprep check via digest Sequencing of cloned PCR product successful: No Joining of validated part to adjacent part(s) status: Not done Problems to be solved: Need to figure out if gel after digest shows correct products

Date: July 17 2008 status report by: Ingrid Part no.: BBa_K110020 Part Description: Venus Enhanced YFP Part Location (in build a genome lab): In silver fridge by door PCR successful?; Yes (http://baderlab.bme.jhu.edu:8888/moodle18/mod/data/view.php?d=4&rid=1471) Cloning of PCR product successful: inconclusive Venus YFP and mCherry Miniprep check via digest Sequencing of cloned PCR product successful: No Joining of validated part to adjacent part(s) status: Not done Problems to be solved: Had some extra unknown products, with unknown bands in the gel Current status of this part: Done with touchdown PCR and gel. Next step is to clone product.

Date: July 17 2008 status report by: Ingrid Part no.: BBa_K110021 Part Description: Venus Enhanced YFP Part Location (in build a genome lab): In silver fridge by door PCR successful?; Yes (http://baderlab.bme.jhu.edu:8888/moodle18/mod/data/view.php?d=4&rid=1471) Cloning of PCR product successful: Inconclusive Venus YFP and mCherry Miniprep check via digest Sequencing of cloned PCR product successful: No Joining of validated part to adjacent part(s) status: Not done Problems to be solved: Had some extra unknown products, with unknown bands in the gel Current status of this part: Done with touchdown PCR and gel. Next step is to clone product.