Transforming Plasmid into Bacillus subtillis


 * Pipette 10μl plasmid to be transformed into 2mL recovery tube.
 * Add 400mL B. subtilis cells. (N.B. These cells must be prepped for transformation by nutrient starvation and heat stress.)
 * Mix by inverting the tube 5 times.
 * Incubate at 37C for 1 hour whilst shaking. To allow sufficient mixing tape the tubes to the rack and place the rack on its side on the shaking table (see diagram below).