Team:Hawaii/Notebook/2008-07- 1

= Things we did today =

PCR gel

 * Margaret


 * Ran 1% gel for oriV, rep, mob, and omega PCR products

Glycerol stocked Biobricks

 * Grace


 * Glycerol stocked B0030, C0012, E0040, J33207, and R0010

Made LB liquid media

 * Grace


 * Two 750ml batches

Plasmid prep

 * Grace


 * Took 1 ml of the overnight Biobrick cultures to inoculate 100 ml LB+amp100. Incubated 8 hours at 37C with shaking (150 rpm). Stored in 4C until tomorrow morning.

Biobrick extraction

 * Grace


 * B0014 (double terminator), plated on LB+amp50+kan20
 * B0015 (double terminator), plated on LB+amp50+kan20
 * E0026 (enhanced cyan fluorescent protein), plated on LB+amp100
 * E0240 (GFP device), plated on LB+amp100
 * I14032 (lac promoter), plated on LB+kan50
 * J04430 (IPTG induced GFP device), plated on LB+amp100

Project description write-ups

 * Margaret & Grace


 * Updated project descriptions on wiki

Secretion project presentation

 * Krystle & Grace


 * Worked on powerpoint and flowchart for Thursday's meeting

Plans for the rest of the week

 * Margaret, Krystle, Grace, and Norman


 * Tomorrow
 * Redo PCR and run on gel (MR)
 * Plasmid prep for B0030, C0012, E0040, J33207, and R0010 (MR)
 * Restock cyanos (KS)
 * Restreak any transformed Biobrick plates from today (GK)
 * Thursday
 * Organize cryostocks (GK)

= Discussion =


 * FYI: Spectrophotometer broken. Make arrangements to use someone else's spectrophotometer if needed.

= Quote of the Day = "GK: 'It depends on if we're dinnering tomorrow.' KS: 'Since when did dinner become a verb?'"