Team:Hawaii/Notebook/2008-07- 9

= Things we did today =

Transformation of DH5a with synthetic oligonucleotide ligations

 * Krystle


 * Followed protocol used for transformation of cells with BioBrick plasmids from filter
 * pilA, slr1, slr2 combined with homemade competent cells (batch 3)
 * pnir combined with commercial supercompetent cells.

Streak Out DB3.1

 * Krystle
 * colony purification for downstream culture-upscaling and preparation of DB3.1 competent cells
 * DB3.1 strain will be used to accept plasmids containing the ccdB "death gene"
 * Sm plate grew, plate lacking antibiotics grew, Kan plate did not grow (as expected)

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Run PCR Products on Gel

 * Margaret
 * PCR amplification of Omega cassette and OriR
 * see here for updates for the pRL1383a amplification

Wiki updates

 * Grace


 * Updated "Protocols" section with new protocols.
 * Updated Initial Oligo Synthesis experiment

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"