Team:Hawaii/Notebook/2008-07-24

= Things we did today =

Verification of parts

 * Krystle


 * Colony PCR of nir+rbs, gfp+tt, gfpfusion+tt transformants
 * Ran 3% gel for colony PCR reactions and plasmid preps of nir, slr1, slr2, pilA, GFP fusion, pRL1383a, B0015, and J04430.

Plasmid prep

 * Krystle


 * Inoculated 7 ml LB+amp with transformants for plasmid prep of nir+rbs, gfp+tt, gfpfusion+tt tomorrow

Sequencing

 * Grace


 * Aligned and checked sequences received from CORE Hawaii for DNA sent in last week

Added BioBrick parts to registry

 * Grace


 * Added BB-pRL1383a, nir, pilA, slr2016, and GFP fusion parts to BioBrick registry

= Discussion =


 * Sequencing for J33207 returned the sequence for E0026 (not sequenced). Need to run all plasmid preps of these two BioBricks on a gel to verify plasmid preps are properly labeled.

= Quote of the Day = ""