Team:University of Ottawa/3 July 2008

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 Home  Welcome  The Team</a>  Who We Are</a>  Advisors</a></li> Undergrads</a></li> </ul> </li> What We've Done</a></li> Where We're From</a></li> Contact Us</a></li> </ul> </li> The Project</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Project_Overview">Overview</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#The_Template">Template</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#The_Design">Design</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Applications">Application</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#References">References</a></li> </ul> </li> <li><a href="http://partsregistry.org/Part:BBa_K149001:Design">BioBricks</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Modeling">Modeling</a></li> <li><a class="MenuBarItemSubmenu" href="http://2008.igem.org/Team:University_of_Ottawa/Wet_Lab">Wet Lab</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Lab_Protocols">Lab Protocols</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/WetWare">WetWare</a></li> </ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Notebook">Notebook</a></li> <li><a class="MenuBarItemSubmenu" href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors">Sponsors</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Acedemic_Sponsors">Academic</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Research_Sponsors">Research</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Corporate_Sponsors">Corporate</a></li> </ul> </li>

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Today in the Lab
Matt
 * Competent Cells
 * <li> Ecoli integrated with ligation products were successful in growing overnight however Atcre did not show any signs of growth
 * <li> The cells containing the ligation product will then be inoculated and left to incubate overnight for a miniprep tomorrow.
 * <li> The Ecoli containing the dehydrogenase component failed to grow from streaking a plate yesterday,I will try and streak again today and then glycerol stock the remaining Ecoli.
 * Ligation
 * <li> Atcre was ligated to circularize the plasmid and will be integrated into Ecoli again today.

Dan
 * Atcre integration
 * <li> Plate had ~4 colonies so I decided to redo integration.
 * 1,2,3,4 and S,D,T ligation
 * <li> The concentration of these constructs was determined to be decent
 * <li> A gel was run to verify the integrity of the constructs, S,D,T showed some contamination
 * <li> 1,2,3,4 did not show up on the gel for unknown reasons.

Dan and Matt
 * Integration
 * <li> Atcre, and 1S 1D 1T 2S 2D 2T 3S 3D 3T 4S 4D 4T were integrated