Wiki/Team:Warsaw/vectors/pTrc99A-AID

pTrc99A-AID vector Description This vector was a kind gift from:

Svend Petersen-Mahrt DNA Editing Laboratory Cancer Research UK Clare Hall Laboratories South Mimms Hert EN6 3LD United Kingdom 44-1707-625770 44-20-7269 3801 Resistance: ampicillin Features: Contains AID (Activation-Induced Deaminase) coding sequnece Usage: All AID constructs were made basing on AID sequence taken from this vector. Was also used in tests for mutagenic activity of AID in E.coli cells Vector map  Vector sequence

gtttgacagcttatcatcgactgcacggtgcaccaatgcttctggcgtca ggcagccatcggaagctgtggtatggctgtgcaggtcgtaaatcactgca taattcgtgtcgctcaaggcgcactcccgttctggataatgttttttgcg ccgacatcataacggttctggcaaatattctgaaatgagctgttgacaat taatcatccggctcgtataatgtgtggaattgtgagcggataacaatttc acacaggaaacagaCCATGGACAGCCTCTTGATGAACCGGAGGAAGTTTC TTTACCAATTCAAAAATGTCCGCTGGGCTAAGGGTCGGCGTGAGACCTAC CTGTGCTACGTAGTGAAGAGGCGTGACAGTGCTACATCCTTTTCACTGGA CTTTGGTTATCTTCGCAATAAGAACGGCTGCCACGTGGAATTGCTCTTCC TCCGCTACATCTCGGACTGGGACCTAGACCCTGGCCGCTGCTACCGCGTC ACCTGGTTCACCTCCTGGAGCCCCTGCTACGACTGTGCCCGACATGTGGC CGACTTTCTGCGAGGGAACCCCAACCTCAGTCTGAGGATCTTCACCGCGC GCCTCTACTTCTGTGAGGACCGCAAGGCTGAGCCCGAGGGGCTGCGGCGG CTGCACCGCGCCGGGGTGCAAATAGCCATCATGACCTTCAAAGATTATTT TTACTGCTGGAATACTTTTGTAGAAAACCATGAAAGAACTTTCAAAGCCT GGGAAGGGCTGCATGAAAATTCAGTTCGTCTCTCCAGACAGCTTCGGCGC ATCCTTTTGCCCCTGTATGAGGTTGATGACTTACGAGACGCATTTCGTAC TTTGGGACTTTCTAGTTAATAAGAATTCGAGCTCCGTCGACAAGCTTggc tgttttggcggatgagagaagattttcagcctgatacagattaaatcaga acgcagaagcggtctgataaaacagaatttgcctggcggcagtagcgcgg tggtcccacctgaccccatgccgaactcagaagtgaaacgccgtagcgcc gatggtagtgtggggtctccccatgcgagagtagggaactgccaggcatc aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt tgtttgtcggtgaacgctctcctgagtaggacaaatccgccgggagcgga tttgaacgttgcgaagcaacggcccggagggtggcgggcaggacgcccgc cataaactgccaggcatcaaattaagcagaaggccatcctgacggatggc ctttttgcgtttctacaaactctttttgtttatttttctaaatacattca aatatgtatccgctcatgagacaataaccctgataaatgcttcaataata ttgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattc ccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctg gtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacat cgaactggatctcaacagcggtaagatccttgagagttttcgccccgaag aacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggta ttatcccgtgttgacgccgggcaagagcaactcggtcgccgcatacacta ttctcagaatgacttggttgagtactcaccagtcacagaaaagcatctta cggatggcatgacagtaagagaattatgcagtgctgccataaccatgagt gataacactgcggccaacttacttctgacaacgatcggaggaccgaagga gctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatc gttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacacc acgatgcctacagcaatggcaacaacgttgcgcaaactattaactggcga actacttactctagcttcccggcaacaattaatagactggatggaggcgg ataaagttgcaggaccacttctgcgctcggcccttccggctggctggttt attgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgc agcactggggccagatggtaagccctcccgtatcgtagttatctacacga cggggagtcaggcaactatggatgaacgaaatagacagatcgctgagata ggtgcctcactgattaagcattggtaactgtcagaccaagtttactcata tatactttagattgatttaaaacttcatttttaatttaaaaggatctagg tgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttt tcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttg agatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccac cgctaccagcggtggtttgtttgccggatcaagagctaccaactcttttt ccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttct agtgtagccgtagttaggccaccacttcaagaactctgtagcaccgccta catacctcgctctgctaatcctgttaccagtggctgctgccagtggcgat aagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggc gcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagc gaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagc gccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcag ggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggt atctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgattt ttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgc ggcctttttacggttcctggccttttgctggccttttgctcacatgttct ttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgag tgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagt gagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatc tgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctct gatgccgcatagttaagccagtatacactccgctatcgctacgtgactgg gtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacg ggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccg ggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagg cagcagatcaattcgcgcgcgaaggcgaagcggcatgcatttacgttgac accatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccgga agagagtcaattcagggtggtgaatgtgaaaccagtaacgttatacgatg tcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaac caggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgat ggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggca aacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcg ccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgc cagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaag cggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaac tatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaa tgttccggcgttatttcttgatgtctctgaccagacacccatcaacagta ttattttctcccatgaagacggtacgcgactgggcgtggagcatctggtc gcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgt ctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatc aaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggt tttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgat gctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccg agtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgat accgaagacagctcatgttatatcccgccgttaaccaccatcaaacagga ttttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctc agggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaa agaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgtt ggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcg ggcagtgagcgcaacgcaattaatgtgagttagcgcgaattgatctg