Waterloo/8 September 2008

080908 Mon

[x] check Sunday's transformation results

Putative good constructs yet to be sequenced: [x] inoculate culture from transformants of parts for sequencing (and making glycerol stock) [x] inoculate culture from transformants of synthesized parts in original GeneArt vector (for glycerol stock)

[x] save glycerol stock of Plac-rfp"

[x] miniprep pSB2K3(gene6) and pSB2K3(PmeI) for diag digest and gel [x] digest of above miniprep [x] gel of above digest [x] for each part, subculture one correct-looking sample in duplicate to kit prep and save stock of tomorrow

[] design crossover PCR primers - Half done

[x] pick time for lab meeting - Meeting has been set for Wednesday Sept. 10th at 5:30