Team:The University of Alberta/1 October 2008


 * James
 * Eco R1 is digested as per Tom's request
 * run a gel: plasmid is ~2000bp so colonies 1-4, w/c are 21-25 are way undersized, that is we expect 5.5kb+2kb=approximally 7.5kb in size
 * ETFA/B is matchs with expected size of 4300bp

David
i transformed competent cells with the following ligations from yesterday pTET+BisdA pTET+BisdB