Imperial College/28 August 2008

= 28 August 2008 =

WetLab

 * Performed midi preps for Terminator, GFP-T, RFP-T and AK3, all had high concentrations and good yields
 * All insert and vector sizes appear to be correct with minimal contamination
 * Double digests with Spe and EcoRI were performed on GFP-T and RFP-T and a 1% agarose gel run. The AK3 and terminator constructs will be digested and run a gel tomorrow morning.


 * Optimised vector genomics and single colony PCR protocols was also performed today. The results of these will be written out in more detail tomorrows notebook.

DryLab

 * Completed production of synthetic video. Updated wiki with regards to generation of synthetic trajectory and bacteria characteristics used to create synthetic video.