Making Frozen Glycerol Cell Stocks


 * Pippette 2μl overnight culture into an Eppendorf tube.
 * Add 0.5ml glycerol and vortex briefly to mix.
 * Prepare a freeze box by decanting a small amount of liquid nitrogen into the box. (N.B: wear protective gloves and face mask.)
 * Place the tube in the box and keep at -80°C.