Team:Mississippi State/PCR Gel

=Making PCR Gel=


 * the Gel is a 1% Agarose gel
 * 0.25g/25ml Agarose/TAE
 * 1) Weigh 0.25g Agarose into flask(Remember weight).
 * 2) Add 25ml TAE.
 * 3) Microwave 1min to boil
 * 4) wait 7min
 * 5) Reweigh
 * 6) Add H2O to difference.
 * 7) Add 5ul EtBr (Wear Gloves)
 * 8) Pour into gel box, inserting templates.
 * 9) When gel dries, remove templates and pour TAE until it reaches top of box
 * 10) Make sure nodes are on back side
 * 11) get ladder (1ul)
 * 12) touch to TAE first, then load first well
 * 13) get 1ul loading dye, place drop on parafilm
 * 14) put 2ul PCR product on drop and mix.
 * 15) load 2nd well
 * 16) put PCR product on ice(-20C)
 * 17) Hook up gel, black-left, red-right; where black is (-) and red is (+).
 * 18) set to 80V for 60min.
 * 19) Remove gel and take to get picture.