Team:PennState/diauxie/progress

background-image: url("http://2008.igem.org/wiki/images/e/e4/Bglogo.png") !important; background-repeat: repeat; }
 * 1) globalWrapper {

.ideasList{ margin-bottom:30px }

ul#sub { margin:0px; padding:0px; font-size:12px; list-style-image:none; list-style-type:none;

}


 * 1) sub li { margin-bottom:20px;}

color:#3300FF; text-decoration:underline; }
 * 1) sub li a {

text-decoration:none; }
 * 1) sub li a:hover {

.links { width:100%; background-color:#000; margin-bottom:30px; }

.links tr { background-color: rgb(155, 230, 159); }

.links td { padding: 0; }

.links a{ display: block; width: 100%; height: 100%; margin: 0; text-align: center; text-decoration: none; font-size: 14px; color: #000; }

.links a:active { }

.links a:hover { background-color: rgb(37,169,113); }

width: 100%; text-align: center; }
 * 1) header {

h4 { font-size: 14pt; font-weight: normal; }

dt { font-size: 12pt; }

padding: .4ex; }
 * 1) projectnav h4 {

font-weight: normal; margin: 1.5ex 0 0 0; }
 * 1) projectnav dt {

text-align: center; text-indent: 0; margin-left: 0; }
 * 1) projectnav h4, #projectnav dt, #projectnav dd {

background-color: rgb(0,80,200); border-left: dashed #FFF 3px; border-right: dashed #FFF 3px; padding: .8ex; }
 * 1) hbnav dd {

background-color: rgb(37,170,113); border-left: dashed #FFF 3px; border-right: dashed #FFF 3px; padding: 1ex; margin-top: 0; }
 * 1) denav dd{

color: #fff; }
 * 1) projectnav a {

text-indent: 1.25em; }
 * 1) pagecontent p {

p.start:first-line { font-weight: bold; letter-spacing: 1.2; }

.progresschecker input{ border-color: rgb(37,170,113); } <! -- END of style sheet -->



These graphs show the normalized fluoresence strength for PN, P1, and P3 xylose promoters induced with xylose, glucose, and a mixture. The W3110 cells have xylE and xylG knocked out while DH5α still contain the natural xylose transport and metabolim. This data shows that there is little effect on the fluorescence intensity using strains with xylE and xylG sequences deleted. Our next step is to transform these promoters into E. coli cells with deleted xylose metabolism and transporters.

The cells were induced iduced with sugars and then allowed to grow with sample being removed every half hour. The goal was to find the induction time where fluorescence starts to level off. The intensity has leveled off and begins to drop after 7.5 hours so this was the growth time for all future tests.

The three promoters analyzed show linear behavior in the optical density range. The strength of fluorescence did change depending on the promoter. With this information we were able to accurately normalize fluorescence data.