Team:Hawaii/Notebook/2008-07-17

= Things we did today =

PCR

 * Grace




 * Colony PCR for GFP fusion brick and new pRL1383a to verify inserts
 * Sequencing (25 mu;l reactions)
 * nir promoter
 * slr2016
 * pilA
 * BBa_B0015
 * BBa_B0024
 * BBa_B0030
 * BBa_C0012
 * BBa_E0040
 * BBa_J04430
 * BBa_J33207
 * BBa_R001
 * Ran all PCR products on a 4% gel to check
 * Gel ran funny -- loading dye ran 2x as fast as the DNA itself
 * 119V too much to handle? Wavy bands
 * Poor ladder resolution on gel
 * No bands seen for GFP fusion, pRL1383a, R0010
 * Incorrect bands for B0015 (multiple bands, what are the two bands at the top?) and J33207 (size too small, stuff still in wells)
 * Will redo plasmid preps for R0010, B0015, J33207
 * Will redo PCR reaction tomorrow for pRL1383a, GFP fusion, B0015, J33207, R0010

Plasmid Prep

 * Grace


 * Inoculated 7 ml LB + amp100 with single colony of B0015, R0010, J04430 for redo of plasmid prep tomorrow

Restreak to Purify

 * Grace


 * Restreaked GFP fusion and pRL1383a (MCS replaced) transformants to purify

pSB3K3 plasmid prep

 * Margaret

= Discussion =

= Quote of the Day = At the team meeting today:


 * "We have cookies, we can start."

Ten cookies later...


 * "The more cookies I have, the better I think!"


 * - Dr. Callahan