Team:Warsaw/Calendar-Main/19 September 2008

MutD5 testing Piotr Testing MutD5 ampicillin resistance - MutD5 isn't resistant to ampicillin

'Hunter and prey' system tests: Competition tests Emilia

 Samples of LB (0.25 mM IPTG; ampicillin 50 μl/ml) was inoculated with following strain combinations: pACYC177+OmpA_A_omega, pACYC177+OmpA_omega and pACYC177+OmpA_Z_omega - A_alpha protein added ~100 μg  pACYC177+OmpA_A_omega, pACYC177+OmpA_omega</a> and pACYC177+OmpA_Z_omega</a> - Z_alpha protein added ~100 μg</li> pACYC177+OmpA_A_alpha</a>, pACYC177+OmpA_alpha</a> and pACYC177+OmpA_Z_alpha</a> - Z_omega protein added ~100 μg</li> </ul> </li>Bacteria centrifuged and frozen </li></ol>

Preparation of &Delta;A (BBa_K103003)</a> Michał K., Piotr <ol>  Gel electrophoresis to estimate concentration of purified DNA from previous day. </li>  Ligation</a> of isolated DNA fragments of pSB1A3</a> and &Delta;A. </li> E. coli TOP10</a> transformation</a> with the above ligation. </li>

Plating on LB + ampicillin.</li>

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