Rensselaer/8 September 2008

DNA purification digest

Fe(13ug) Plasmid(11ug)

DNA    280ul     95ul

XbaI   10        10

SprI   20        20

Buffer2 40       17.5

BSA    4         1.75

dH2O   46        30.75

____     _____

400 ul    175 ul

Insert checks digest

Fe-mRFP (~1ug)

DNA(30ug/ul)   33

MsCI(3U/ul)    6.7

EcoRI(2U/ul)   1

EcoBuff        5

BSA            0.5

dH2O           3.8

_____

50 ul