Waterloo/10 July 2008

08/07/10 Thurs

[x] competent cells (finish)

[x] gel continued from yesterday (PCI (1-3), Pconst (1-2), PtetR (1-2), PLacOCI(1-3), TTr(2,5) and uncuts)

[x] gel purification and extraction of rfp' and tt, rbs and cI

[x] make 2 L of LB media

[x] ligation of rfp' and tt, cI (coo51) and rbs, ci (coo52) and rbs - transformation tomorrow morning

- repeat diagnostic digestion frozen in the -20 to run on gel tomorrow.

- Strain of P. mendocina came in (KR1) not sure if it contains REN gene. - Plated on LB, and innoculated liquid media for glycerol stock

- weef presentation