Team:University of Ottawa/25 June 2008

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 Home  Welcome  The Team</a>  Who We Are</a>  Advisors</a></li> Undergrads</a></li> </ul> </li> What We've Done</a></li> Where We're From</a></li> Contact Us</a></li> </ul> </li> The Project</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Project_Overview">Overview</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#The_Template">Template</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#The_Design">Design</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Applications">Application</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#References">References</a></li> </ul> </li> <li><a href="http://partsregistry.org/Part:BBa_K149001:Design">BioBricks</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Modeling">Modeling</a></li> <li><a class="MenuBarItemSubmenu" href="http://2008.igem.org/Team:University_of_Ottawa/Wet_Lab">Wet Lab</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Lab_Protocols">Lab Protocols</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/WetWare">WetWare</a></li> </ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Notebook">Notebook</a></li> <li><a class="MenuBarItemSubmenu" href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors">Sponsors</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Acedemic_Sponsors">Academic</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Research_Sponsors">Research</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Corporate_Sponsors">Corporate</a></li> </ul> </li>

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Today in the Lab
Matt
 * PCR Amplification
 * <li> F60, F61 primers used for PTP2 cassette. I also labelled all the primers we received that we will be using for the next few months.
 * <li> I decided to use the Kaern Lab protocol for PCR Amplification.
 * <li> The PTP2 PCR product was then run on a gel - gel results were as expected with 2275 size band.
 * Glycerol Stock
 * <li>Glycerol stock of plasmids D12, S1, T123, pSS042 with LB + glycerol was performed.

Dan
 * Ran gel of pSSA42, S1, D12, T123 plasmids
 * <li> This time the marker was added and expected results were obtained. SB, DA, TB, and 42B colonies were chosen for glycerol stock.
 * Cleaned out DNA box
 * <li> Cleaned out all DNA that will not be used from our box
 * <li> Our names are getting complicated so I am renaming our DNA using the following legend (all other DNA was discarded)
 * {| class="wikitable"


 * Plasmid || Strain || New Name
 * 587 || 7B || 7
 * 598 || 8B || 8
 * 600 || 0A || 0
 * 601 || 1C || 1
 * S1 || SB || S
 * D12 || DA || D
 * T123 || TB || T
 * pSSA42 || 42B || 42
 * }
 * PCR amplification of S, D, T, 7 and 8
 * <li> S, D, and T plasmids were PCR amplified overnight using primers F69 and F70
 * <li> PCR program for 0&1 was: PHUSION
 * <li> PCR program for S&D&T was: PHN1
 * <li> The 0 and 1 plasmids were amplified according to the following chart: (Primer F66 is :Int TetR Repl F (*PstI) (B). primer F67 is Int GFP Repl F(*SphI) (A), and primer F68 is:Int SSRE R(*ClaI )
 * {| class="wikitable"
 * pSSA42 || 42B || 42
 * }
 * PCR amplification of S, D, T, 7 and 8
 * <li> S, D, and T plasmids were PCR amplified overnight using primers F69 and F70
 * <li> PCR program for 0&1 was: PHUSION
 * <li> PCR program for S&D&T was: PHN1
 * <li> The 0 and 1 plasmids were amplified according to the following chart: (Primer F66 is :Int TetR Repl F (*PstI) (B). primer F67 is Int GFP Repl F(*SphI) (A), and primer F68 is:Int SSRE R(*ClaI )
 * {| class="wikitable"
 * {| class="wikitable"


 * Sample || F66 || F67 || F68
 * 0A ||   || Y || Y
 * 1A ||   || Y || Y
 * 0B || Y ||   || Y
 * 1B || Y ||   || Y
 * }
 * 0B || Y ||   || Y
 * 1B || Y ||   || Y
 * }
 * 1B || Y ||   || Y
 * }