Team:Hawaii/Notebook/2008-09- 2

= Things we did today =

Plasmid prep

 * Grace & Krystle


 * BB-pRL1383a
 * GFPf + tt colonies #8, 17, and 21

Colony PCR

 * Grace


 * Colony PCR of 3A ligation transformants

PCR

 * Grace


 * J33207 with HindIII/BamHI primers

RE digest

 * Grace


 * J33207 and BB-pRL1383a with HindIII and BamHI

Made media plates

 * Grace


 * 1 sleeve of LB+sp2.5+sm2.5
 * 2 sleeves of LB+amp100

Construction of GFP + TT

 * Krystle


 * Ligated GFP (cut with SP) with tt (synthesized) using T4 ligase
 * negative control: re'd GFP ligated to self

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"