Team:Warsaw/Calendar-Main/4 August 2008

Cloning of truncated fragment of protein A (&Delta;A) Piotr Inoculation of some pACYC177+OmpA_alpha + &Delta;A and pACYC177+OmpA_omega + &Delta;A colonies of tranformants.

Checking OmpA_omega_&Delta;A_alpha expression Piotr

Inoculation of OmpA_omega_&Delta;A_alpha with inductor (0,5 mmol/mL IPTG) and negative control without IPTG.

Checking if OmpA_omega_&Delta;A_alpha gives ampicillin resistance Emilia

Inoculation of OmpA_omega_&Delta;A_alpha into various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75, 1 mmol/mL.

Preparing pACYC177+OmpA_omega_&Delta;A construct Michał K. Digest of pACYC177+OmpA_omega_&Delta;A_alpha</a> (from 25 July) with BamHI and NotI (BamHI buffer). DNA ends blunting</a> with Klenow fragment (3 hr). </li> <li>Gel elctrophoresis (Fig. 1</a>) and gel-out</a> of proper band - 4300 bp. </li> <li>Overnight ligation</a> of isolated DNA fragment. </li> </ol>

<img src="http://2008.igem.org/wiki/images/1/17/Gucio.jpg" width=240/></a> Fig. 1. Gel-out of pACYC177_OmpA_omega_deltaA_alpha digested with NotI/BamHI after blunting ends. 1. Marker 2. pACYC177_OmpA_omega_deltaA_alpha