Alberta NINT/14 July 2008

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lab work (SD): Growth rates of cells containing the plasmids K102022, K102032, K102032+K102011, K102032+K102034, K102032+K102035, K102032+K102036 and XL1-B were to be determined by measuring the cell count every hour for cells grown at 30 and 37 C. Growth was only observed in overnights for K102022 and XL1-B. t=1 hr    t=2 hr     t=3 hr     t=4 hr             LB               0.000      0.000      0.000      0.000 K102022 (30 C)  0.128      0.162      0.192      0.247 K102022 (37 C)  0.167      0.278      0.351      0.407 XL1-B  (30 C)   0.153      0.166      0.194      0.230 XL1-B  (30 C)   0.147      0.238      0.344      0.345

lab work (JD): Nothing grew from Friday's ligations so Wayne re-tranformed my ligations and carried out his own ligations Plate - # colonies K102031W - - 2 K102021W - - 1 NegativeW- - 2 K102031J - - 0 K102021J - - 0 NegativeJ - -0 Obviously, weekends ligations didn't work either.