Edinburgh/5 August 2008


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Tuesday 5 August 08

 * M72 (pSB1A2+rbs+dxs), M79 (BABEL2+crtE) and M82 (pSB1A2+rbs+appY) submitted for seqencing. (CF)
 * Minipreps M97-M104 of pSB1A2+cenA (plate 85, steaks 2 and 7-13) and M105-M108 of pSB1A2+cex (plate 86) were made. (Yan)
 * M97-M108 were double-digested with EcoRI/PstI and run on gel 37. The results do not look good except for M97. (Yan)
 * Purification and self-ligation of P50 (3rd mutagenesis of glgc-mut1,2) (L30). (HX)
 * M68-M69 (pSB1A2+glgc-mut1,2) and M92 (pSB1A2+cex) were digested with a) PstI and b) EcoRI/PstI, but gel leaked. Entire process needs repeating tomorrow! (CF, OG)
 * Digested P36 (glgC-mut1,2) with Edinbrick1 using Spe1/EcoR1 and ligated (L31). (OG)


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