Alberta NINT/21 July 2008

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lab work (JD): Replated cells gave us more cells on the weekend: K102031: 4 K102021: 4 Negative: 0 Wayne made O/N of these cells as well. Mini-preps of K102031.1-K102031.4 and K102021.1-K102021.4 Sequencing reaction for all 8 and dropped off at MBSU

lab work (SD): Results from 18/07/08 Transformation: 1/100     4/100      1/50       2/50             LB+amp50        8        20         1          10 LB+kan35       6        15         0           8 LB+amp50+kan35 0         0         0           0 The concentrations of the DNA for the plasmids used must be checked as these are lower counts than expected. Inoculated a 96-well plate with overnight cultures in preparation for the lacZ assay.