Purdue/18 September 2008

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Digested SOS plasmids:
 * Mixture:
 * 40uL DNA
 * 5uL NEB Buffer 2 (10x)
 * 13uL ddH2O
 * 0.5uL BSA
 * 1uL EcoRI
 * 1uL SpeI
 * Mixed, stored at 37C for 1 hour
 * Placed in -20C to halt reaction

We'll run it through a gel and purify the SOS and LacZ parts soon. Then ligation, transformation, testing!

Edited by Janie Stine