Team:Rice University/Notebook/11 July 2008


 * David Ouyang
 * Digest and Ligate Putative Amber Suppressors
 * Digest Mix
 * 1uL EcoRI
 * 1uL PstI
 * 5uL NEB3
 * 20uL Dilute DNA
 * 1uL BSA
 * 22uL dH2O
 * DCC
 * Ligation Mix
 * 10uL DCCed DNA
 * 1uL T4
 * 2uL Buffer
 * .5uL Vector
 * 7.5 dH2O


 * Taylor Stevenson
 * Prepare 5mL LB O/N of VCS257 (37*C) and VCS257 lambda lysogens (30*C) for preparation of electro-competent cells

