Alberta NINT/13 August 2008

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lab work (SD): Isolated DNA of TA2, TA3, TA6 and Ta7 from overnights using QIAquick spin miniprep standard protocol. Sequenced TA2, TA3, TA6 and TA7. Made overnights of the three colonies found on the pSB1A3 plate.

lab work (JD): From ligations: TA2In=76 colonies TA3In=90 colonies TA6In=66 colonies TA7In=68 colonies Negative=4 colonies (from Wayne) Created 4 O/N from each plate Annealed TA2, TA3, TA6, TA7 Nucleotide removal kit for TA2 TA3 TA6 TA7 Restriction Digest of TA2, 3, 6, 7 with BamH1 and NcoI Ran on gel 2% at 150V for 2 hours