Team:Chiba/jk/β/week 2

>送受信班

>Protocols

24 August, 2008

 * (22/8)--->Mini prep
 * BBa_J04500(2007)
 * BBa_J04500(2006)
 * BBa_J13002(2007)
 * BBa_J13002(2006)


 * --->Digestion test
 * BBa_J04500(2007)
 * BBa_J04500(2006)
 * BBa_J13002(2007)
 * BBa_J13002(2006)


 * --->Gel Check

25 August, 2008

 * (24/8)--->Digestion
 * Double Digestion:BBa_J04500(2007)
 * Double Digesiton:BBa_R0010(2007)
 * Single Digestion:BBa_R0010(2007)
 * BBa_R0010(2007)


 * --->Gel Check

--->Digestion (Double Digestion)
 * BBa_C0178
 * BBa_C0170
 * BBa_J04500(2007)

26 August, 2008

 * (8/25)--->Gel Check

--->Gel Extract


 * --->Zymo Clean
 * 5μl
 * 5μl
 * 27μl


 * --->Gel Check


 * --->SAP


 * --->Zymo Clean
 * Sample No.3 -->9μl


 * --->Gel Check


 * --->Ligation


 * --->Transformation
 * insert①C0178
 * insert②C0170
 * vector③J04500
 * insert①C0178+vector③J04500
 * insert②C0170+vector③J04500

27 August, 2008
Digestion Test
 * Sample DNA : BBa_R0010


 * --->Gel Check


 * (26/8)--->Colony-PCR
 * Colony PCR of 5 colonies from ligation plates (26/8-(4),(5)) and one from control plate(BBa_F2620(2007)).




 * 95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃


 * --->Gel Check


 * --->(28/8)Miniprep

Transformation
 * competent cells : XL10G
 * BBa_C0062(2007)
 * BBa_C0062(2006) -> no colony on plates
 * BBa_C0179(2007)
 * BBa_C0179(2006)
 * BBa_S03156(2007)
 * BBa_S03156(2006)
 * BBa_S03158(2007)
 * BBa_S03158(2006)
 * BBa_S03160(2007)
 * BBa_S0316(2006)


 * --->Mini prep


 * --->(29/8)Gel Check

28 August, 2008

 * (27/8)--->'''Mini prep
 * insert:C0170 + vector:J04500
 * insert:C0178 + vector:J04500


 * --->Digestion Test
 * insert:C0170 + vector:J04500
 * insert:C0178 + vector:J04500


 * --->(29/8)Gel Check

29 August, 2008

 * --->Digestion Test
 * BBa_R0010(2007)
 * BBa_C0062(2007)
 * BBa_C0179(2007)
 * BBa_C0179(2006)
 * BBa_S03156(2007)
 * BBa_S03156(2006)
 * BBa_S03158(2007)
 * BBa_S03158(2006)
 * BBa_S03160(2007)
 * BBa_S03160(2006)


 * --->Gel Check


 * (27/8)--->Gel Check

Digestion
 * BBa_I9026(2007)
 * BBa_I9030(2007)
 * BBa_S03154(2007)
 * BBa_R0010(2006)


 * --->Gel Check


 * --->Gel Extract


 * --->Zymo Clean
 * ->15μL


 * --->SAP


 * --->Zymo Clean


 * --->Suspension! (We got information, by the team-output, to the effect that the density of the sample is too low.)

30 August, 2008

 * Transformation
 * competent cells : XL10G
 * BBa_I9026(2007)
 * BBa_I9030(2006)
 * BBa_S03154(2007)
 * BBa_S03156(2007)
 * BBa_S03158(2007)
 * BBa_S03160(2007)
 * BBa_C0062(2007)
 * BBa_C0179(2007)


 * --->(31/8)Mini prep


 * (29/8)--->Mini prep
 * insert:C0170 + vector:J04500
 * insert:C0178 + vector:J04500


 * --->Digestion Test
 * insert:C0170 + vector:J04500 -> Sample No.1~4
 * insert:C0178 + vector:J04500 -> Sample No.5~8
 * BBa_T9002(2007) -> Sample No.9
 * Single Digestion of Sample No.1~9 -> Sample No.10~18
 * Double Digestion of Sample No.1~9 -> Sample No.19~27




 * --->Gel Check