Team:University of Ottawa/14 July 2008

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Today in the Lab
Matt
 * PCR Amplification
 * <li> Corey decided it would be best to start from the beginning and amplify the PTP2 out of Genomic DNA again. I redid the PCR and I am running it overnight.
 * Inoculation
 * <li> 97 plasmid was inoculated from master plate so that it can glycerol stocked for tomorrow.
 * PCR Confirmation
 * <li> Corey wanted me to do another PCR confirmation of the 97 plasmid with primers F58,F59. Expecting a band size of 2200 for this PCR when I run it on a gel for tommorrow.

Chris
 * Digestion of AtCRE (again)
 * <li> AtCRE was digested with EcoRI again for one hour at 37 C
 * <li> the result was run on a 1% gel, resulting in five bands where only two were expected.
 * <li> using Vector NTI, we figured out that the incorrect enzyme was used for the digestion; in fact, EagI should have been used instead.

Dan
 * PCR amplification of construct 1
 * <li> The amplification product was run on a gel however no clear bands showed up on the kodac machine. However the correct band was seen on the cutting UV machine.
 * <li> The band was extracted using the Sigma kit and eluted with water.
 * <li> Absorbance measurements indicated that no DNA was obtained, next time elution solution at 65 C will be used
 * <li> PCR amplification of 0A and 0B was run overnight.