Team:University of Ottawa/24 July 2008

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Today in the lab
Chris
 * Double-Check of AtCRE
 * <li> Digested samples showing desired bands with EcoRI for one hour.
 * <li> Ran against a water control and DQ2325601 digested with EcoRI for one and a half hours on a 1% gel at 80V
 * <li> One of two samples still showed desired bands following double-check. This sample was streaked onto a master plate, which was incubated at 37 C overnight, and glycerol stocked in the -80 C fridge for further use.

Matt
 * Ligation Results
 * <li> A gel was run after two hours for ligation of PTP2/pSSA42 - unsuccessful.
 * <li> A gel was run with overnight ligation - vector and H2O controls were clean and ligation product band is slightly visible in 3:1 ratio. However there is auto ligation occuring with the vector.
 * PCR
 * <li> Dan and I decided that the initial extraction of PTP2 after PCR yielded a product that was too concentrated to be properly extracted - so I am redoing the PCR amplification with elongation time raised to 1 min 15 sec and annealing temp raised to 58 C.
 * <li> I will also spread the PTP2 amplification product over many different lanes on the gel so that it can be properly extracted.
 * Digestion
 * <li> pSSA42 was redigested with BamHI and xhoI.

Dan
 * 'Wet Ware
 * <li> Worked a little bit on trying to get some wet ware tools working.
 * 'Innoculation
 * <li> Innoculated 6 colonies and a control