Team:Rice University/Notebook/7 July 2008


 * David Ouyang
 * Ran a gel of:
 * GFP overnights (over long weekend) were miniprepped
 * PCR product of SupF


 * Did quikchange of GFP to create Amber Suppressed GFP.
 * PCR
 * 5uL Buffer
 * 1uL RFP (60ish ng/mL)
 * 1uL dNTP
 * 2.5uL QC-RFP-F
 * 2.5uL QC-RFP-R
 * 1uL PfuTurbo
 * 37uL dH2O

First lane: 1kb ladder

Second lane: [ptet][RFP] plasmid, supercoiled? Should be around 6 or 7. Faint band around there.

Third lane: 100bp ladder

Fourth and Fifth lanes: +- DMSO, Colony PCR of SupF. Theoretical length (170). Should be around where the bottom band is. Second band could be artifact from genomic.