Team:Warsaw/Calendar-Main/17 July 2008

Cloning of protein Z DNA to OmpA constructs Michał K.    Digest and dephosphorylation (with CIAP) of pACYC177+OmpA_alpha with SacI and NotI (BamHI buffer).   Gel eloctrophoresis and gel-out (4300 bp band).   Ligation of isolated DNA fragment and Z DNA fragment isolated on 14 July</a>. </li>  Transformation</a> of E. coli TOP10</a> strain with ligation. </li>  Transformants plating on LB + kanamycin. </li> </ol>

Cloning of protein Z DNA to pET15b-OmpA-omega</a> in place of OmpA Paweł <ol>Digest</a> of pET15b-OmpA-omega</a> and Z (in Geneart vector</a>) with NdeI and NotI (Orange buffer).</li> Gel-out</a> of Z (~200 bp band).</li> Overnight ligation</a> of Z into digested pET15b-OmpA-omega</a>.</li>

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