Team:Warsaw/Calendar-Main/30 June 2008

Change of the reporter from pZC320 with B-galactosidase to GFP or RFP Piotr, Weronika  There were blue and white colonies but no red or green in UV.

Inoculation of liquid LB medium + Amp with white colonies. Inoculation of 10ml LB with E.coli TOP10 carrying pZC320. Inoculation of E.coli TOP10 carrying pSB1A2 standard plasmids carrying parts: BBa_E0840(GFP genetrator) and BBa_J04450</a> (RFP generator).

</li></ol>

Cloning alpha-A and omega-A fusions on pKS</a> Michał L., Ewa, Marcin

Inoculation of liquid LB medium with 6 transformant colonies.

</ol> Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega Paweł Inoculation of  E. coli with <A href=http://www.emdbiosciences.com/docs/docs/PROT/TB045.pdf>pET15b</a> plasmid on LB + ampicillin.