Team:Hawaii/Notebook/2008-09- 4

= Things we did today =

Re-replacement of BB-pRL1383a MCS

 * Grace
 * Ran last night's RE digest on agarose gel
 * Extracted J33207 and BB-pRL1383a from gel
 * Treated BB-pRL1383a w/ SAP
 * Checked DNA concentration of BB-pRL1383a (---ng/&mu;l)
 * Ligated J33207 with BB-pRL1383a
 * Negative controls = BB-pRL1383a ligated to self, J33207 ligated to self
 * Transformed into DH5&alpha; cells

3A construction of plac+rbs

 * Grace


 * Ligated:
 * C0012V (SAP treated) + plac + rbs (B0034 back ligation)
 * C0012V + plac + rbs (B0034 back ligation)
 * Negative controls = C0012V (SAP treated and no SAP treatment) ligated to self, plac + rbs ligated w/o vector
 * Transformed into DB3.1 cells

Cont'd construction of GFP + TT

 * Krystle
 * Transformed negative control and ligation product from Tuesday into DB3.1

Construction of B-H-R Vector Intermediates

 * Margaret


 * Ran a gel of Yesterday's restriction digest
 * Purified the bands that were the correct size, will re-do the others.
 * Set up cultures of constructs that did not appear on the gel.

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"