Team:Chiba/jk/β/weak 5

>送受信班

14 September, 2008

 * (13/9)--->Mini prep


 * Transformation
 * Competent cells : XL10G
 * BBa_T9002(2007)
 * Plac+RhlI+LVA
 * Plac+CinI+LVA


 * --->(15/9)

15 September, 2008

 * Transformation
 * competent cells : BW⊿FliC
 * BBa_S03623
 * BBa_K084007
 * BBa_K084008
 * BBa_K084009
 * BBa_T9002

(15/9)->cultured in new LB-Amp medium.  added AHL(total concentration was 0nM,1nM,10nM,100nM,1000nM)
 * -->check green fluorescence intensity(Excitation:485nm,Emission:527nm)

-->T9002 protocol

Cultured transformants(LB,37℃,14h) cultured above in new LB medium. -->mix(16/9)
 * BBa_K084007~BBa_K084009
 * BBa_T9002
 * BBa_S03623

16 September, 2008
(15/9)->mix cultures in the way prescribed in the Table below.
 * -->check green fluorescence intensity(Excitation:485nm,Emission:527nm)