Team:Chiba/Calendar-Home/25 September 2008

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24 September 2008 <|> 26 September 2008

Team:Output
colony PCR Vector:BBa_R0062 Insert:BBa_E0240 Vector:BBa_R0062

->95&deg;C 5 min ->(95&deg;C 1 min 50&deg;C 30sec　72&deg;C 1 min)&times;25 ->72&deg;C 10 min

Digestion test BBa_I6206 BBa_I6101 BBa_I6042 BBa_I0462

->30 min 37&deg;C gelcheck BBa_I6206(digestion product) BBa_I6101(digestion product) BBa_I6042(digestion product) BBa_I0462(digestion product) BBa_I0642(natural)

digestion BBa_I0462

->3 hours 37&deg;C ->Zymo ->10&mu;L

Ligation Vector:BBa_I7100 Insert:BBa_R0062 Insert:BBa_I0462

->3 hours 37&deg;C ->Transformation