Team:Hawaii/Notebook/2008-08-10

= Things we did today =

Checked DNA products via gel electrophoresis

 * Grace
 * Ran RE digests on an EtBr stained 2% agarose gel at 72V for 100 min.
 * Picture really dark. Adjust camera? Restain with more EtBr tomorrow?
 * Resuspended plasmid preps from yesterday in 50 &mu;l TE buffer and ran 5 &mu;l on an EtBr stained 1.2% agarose gel at 95V for 1 hour
 * Plasmid prep for I14032 (2008 distribution) was discarded. Solution was very thick (like egg whites) during resuspension, indicating high levels of proteins present.
 * Plasmids didn't run. Still really close up to to wells. Trouble shoot tomorrow.

Cryostocks

 * Grace


 * E. coli did not grow. Reinoculate tomorrow.

= Discussion =

= Quote of the Day =