Team:iHKU/Notebook

Wet lab log book for the biobrick subgroup
(Many Thanks to MIT team for setting a good example for us ^_^)


 * Click on any day below to see what wet-lab procedures were conducted.


 * log book summary

0703 done:  punched: and transformed. BBa_Q04510   BBa_R0062  BBa_R0011 BBa_I732096

Planning and summary section for the biobrick subgroup
here is the Biobrick subgroup progress,


 * Roy has got these following biobircks:

Part:BBa_E0240  Colony   standard measurement for promotor

p1010 psb3K3


 * parts already obtained by peter：

Part:BBa_F2622  Colony

Part:BBa_T9002  Punch Part:BBa_I731007   Punch

Part:BBa_B0030  Punch

S17 strain colony ready for ccdB


 * parts we further need to get

Part:BBa_Q04510 1013 7A  Transf

Part:BBa_R0062 1000  4G  Transf

Part:BBa_R0011 1000 4b Transf

BBa_I732096 1014 3G  Transf reporter containing the standard GFP and LacZa


 * parts that may be useful

http://partsregistry.org/Part:BBa_B0031

http://partsregistry.org/Part:BBa_B0032

http://partsregistry.org/Part:BBa_B0033

http://partsregistry.org/Part:BBa_B0034


 * parts that we need to build:

1 Part:BBa_K094001CheZ  assumed without rbs

2 Part:BBa_K094002LuxRI  assumed with rbs and terminator


 * composite we need to construct:

1plaLuxRpluxCheZGFPLva BBa_F2622 BBa_B0030 BBa_K094001 BBa_B0030 BBa_E0022 BBa_B0015 （something wrong with GFPLva and i will change them later）

2plaLuxRpluxCIpldCheZGFPLva BBa_F2622 BBa_Q04510 BBa_B0030 BBa_K094001 BBa_E0430

3plaLuxRpluxLacZ BBa_F2622 BBa_B0030 BBa_I714901 BBa_B0015

4plaLuxRpluxCIpldLacZ BBa_F2622 BBa_Q04510 BBa_B0030 BBa_I714901 BBa_B0015

5plaLuxRIpluxCheZGFPLva BBa_R0011 BBa_K094002 BBa_R0062 BBa_B0030 BBa_K094001 BBa_E0430

6 first

7 Part:BBa_K094009

parts entered on 080822

11

12

13

14

15

16

17

18

Notebook
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