EPF-Lausanne/6 October 2008

<>

Cloning
Results of the plating of RBS-LuxI-Term:

40 colonies, and 6 on the vector autoligation.


 * 8 colonies picked and miniprep'd.
 * Ctrl. digestion with X,P.

Expected bands:
 * 2.1kb (vector pSB1A2)
 * 800bp (luxI by 2step pcr)

Clones 2, 3, 5 and 8 are correct.

Cloning of A1 into B1:

Cloning was succesful.