Alberta NINT/17 July 2008

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lab work (SD): Since the double-plasmid cell cultures did grow on the LB+kan35 plates we will try again to             grow them on LB+amp+kan35. This time, 2.0ul of each of the plasmids is transformed into XL1-B cells and plated in an attempt to increase transformation efficiency. K102032+K102011 (LB+amp50+kan35) K102032+K102034 (LB+amp50+kan35) K102032+K102035 (LB+amp50+kan35) K102032+K102036 (LB+amp50+kan35)

lab work (JD): Gel purification of pSB4A5/E+P, K102003/E+P, K102006/E+P Quantitated the samples Carried out ligations: K102003+pSB4A5=K102031, and K102006+pSB4A5=K102021 Transformed XL1-B Cells Plated cells on LB+Amp plates