Team:Hawaii/Notebook/2008-09- 9

= Things we did today =

Cryostock

 * Grace


 * nir+rbs
 * rbs+GFP

Plasmid prep

 * Grace


 * nir+rbs
 * rbs+GFP
 * plac+rbs
 * slr1+GFPf
 * pilA+GFPf (8/12 and 8/13 transformations)

PCR

 * Grace
 * nir+rbs
 * rbs+GFP
 * plac+rbs
 * slr1+GFPf
 * pilA+GFPf (8/12 and 8/13 transformations)
 * slr1
 * pilA

Verified Krystle's PCR products from yesterday

 * Grace



RE digest

 * Grace


 * EcoRI and SpeI:
 * nir + rbs
 * plac + rbs
 * XbaI and PstI:
 * slr1
 * pilA
 * slr1 + GFPf
 * pilA + GFPf (8/12)
 * pilA + GFPf (8/13)
 * EcoRI and PstI:
 * pSB1A3

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"