Team:Hawaii/Notebook/2008-06-26

= Things we did today =

Large Scale Plasmid Prep

 * Margaret, Krystle
 * Found concentration using spectrophotometer
 * Ran gel but DNA did not migrate.
 * Restriction digest of prep with EcoRI and ran gel.

Cell density count

 * Grace


 * Completed cell density count experiment

Purified triparental conjugants

 * Grace


 * Restreaked transformed PCC6803 on new BG-11+sm2.5+sp2.5 plates for purification

Timeline for Secretion Project

 * Grace, Krystle


 * Planned timeline (still in works) for secretion project
 * Will convert pRL1383a into a BioBrick format plasmid so testing in PCC6803 can begin ASAP

Large Scale Plasmid Prep

 * Margaret


 * I am updating the wiki with the details of this experiment: the how to and the what went wrong.

= Discussion =

= Quote of the Day = "Oooh, look at the pretty colors - Krystle, refering to the agarose gel"