Team:Waterloo/Notebook/Protocols/Ligation

 Ligation (10µL recipe with 5 insert : 1 vector ratio) 

Materials  MQ water Ligase buffer Ligase Eppendorf tube Vector DNA Insert DNA

Instructions  1. Calculate ligation volumes. Total volume should be 10 µL and insert to vector ratio between 3:1 –5:1. 2. In one tube mix - Vector DNA - Insert DNA - 10× ligase buffer - Ligase - MQ if needed 3. Leave on the bench for 1 hour (or even overnight)