Protocols E.coliTransformation

Cocktail: 70 ul d.s.H2O 10 ul Ligation product 100 ul Competent cells

___________________ 180 ul Total

Control: 70ul d.s.H2O 10ul ligation product 100ul Competent cells

___________________ 180ul Total

Procedure: Let product sit on ice for 10 minutes Take out plates with corresponding antibiotics Label plates Heat shock in 42*C water bath for 1.5 minutes Put on ice for 5 minutes Turn on Bunsen Burner Pipette all cells and put on plate Spread cells on plate with streaks for cell/colony dillution Put in 37*C incubator lid facing down for 8 hours or overnight

Protocols