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Hello 6/29/2009 R. palustris TIE-1 and S. oneidensis WT strains appear to have grown. Gram staining of S. oneidensis WT revealed what appeared to be gram-negative rods; primers being designed for a unique S. oneidensis sequence to confirm species is what we suspect. Plan now to: - inoculate liquid media with single colonies from these strains. -Inoculate - Re-streak individual colonies from these strains, along with TIE-3 (R. palustris) and MtrB and OmpB strains (S. oneidensis)

Tomorrow: -Order designed primers -Grow mutants in liquid media

6/30/2009 Grew mutants alongside WT Shewanella. They don't look alike. I'm suspicious.