Purdue/17 June 2008

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 * First trial of nebulized Sol-Gel
 * Coated bottom of petri dish w/ media (alpha-MEM)
 * Sprayed for 45s
 * Added 4 mL media and 1 mL LB afterwards
 * Wait to see if the cells grow
 * Got visual confirmation via microscope
 * GFP E. coli are alive
 * Sol-Gel is flaking off
 * Next time attempt with bottom layer of gelatin so Sol-Gel doesn't flake/leak/etc.

Side note: We were mistaken about the sam8 gene--it is the LacZ gene that encodes Beta-gal which cleaves X-gal to make a blue color. The sam8 is irrelevant. LacZ can be found as part I732005, and is available in the 2008 shipment of DNA.

Edited by Janie Stine