Team:Warsaw/Calendar-Main/8 July 2008

Preparation of constructs: OmpA_alpha and OmpA_omega #2 Piotr   Transformation of E. coli TOP10 strain with ligation from previous day.   Transformants plating on LB + kanamycin.

Cloning omega-A fusion on pKS (second attempt) Michał L., Ewa, Marcin  Gel electrophoresis of PCR products. <a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Gel-out</a> of proper band.</li> <a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcl">PCL</a> reaction to create omega-A fusion:

</li> Gel electrophoresis of PCL products.</li> We have obtained no PCL product (weird?).</li>

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