Team:NTU-Singapore/Notebook/26 June 2008



  

=Thursday 26 June= {|border="1" style="background-color:#ffffcc;" cellpadding="20"

10:30am to 12:30pm:

 * Min-Lin, Hung: miniprep (with new kit) for:
 * GFP self-ligated plasmid (control)
 * GFP producer E0480
 * Choon Kit: nanodrop for digested Fe, GFP to evaluate the amount for ligation.

2pm to 4pm:

 * Choon Kit: ligation of pFE and GFP (with some new improvements), using 2 pFe:GFP ratios (1:4 and 1:7)
 * Hung:
 * Prepare digestion mixture for GFP self-ligated and GFP E0480
 * Loading gel for GFP self-ligated, GFP E0480, Fe-GFP (1:4) and Fe-GFP (1:7)

4:30pm to 7pm:

 * Choon Kit, Hung: transformation + cloning for:
 * Fe-GFP (1:4) and Fe-GFP (1:7) : using commercial top 10
 * LacI-GFP: using homemade top10
 * Min-Lin: inoculation (2 tubes for each plate) for pFe, pT7, Terminator, empty plasmid with AmpR, RBS32, RBS34, GFP 0240, LacI-GFP (in BL21), LacI.