Team:NTU-Singapore/Notebook/29 May 2008



  

Wednesday
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ChinChong

 * Requested E coli strain B2B110 with the ColE7 plasmid from Professor David Gordon (Australia)
 * Requested quotation from Invitrogen on some chemicals and kits
 * Discussed possible experiments design to patch up the parts of our BioBrick
 * Finding parameters for the modeling from literature

Darius

 * Come up with provisional LacI experiment design
 * Discussed possible experiments design to patch up the parts of our BioBrick

Choon Kit

 * Plasmid Extraction using Qiagen Miniprep. Ligation using EcoRI and PstI
 * Alias with Professor Yuan Hanna (Taiwan)to get E7+Imm plasmid, expected arrival 5th Jun 08
 * Alias with Professor Chris Viogt (UCSF) to get AND modular plasmids, expected arrival 5th Jun 08
 * Requested full DNA sequence of ColE7 and Imm plasmid from Zhong Zhi Hao (Taiwan)
 * Requested full DNA sequence of AND modular plasmids from Chris Voigt (UCSF)
 * Documented laboratory work for 28 and 29 May 2008

Hung

 * Plasmid Extraction using Qiagen Miniprep. Ligation using EcoRI and PstI (basically phase II and up to step 3 of phase III of experiment 3 for iron promoter. We left the DNAs incubated overnight instead of for 2hrs due to time constraint.)
 * Finding parameters for the modeling from literature

ZhenFu

 * Improve our group's model with some literature parameters
 * Added equations to our model
 * Puzzled over results of simulation, shall work on it further with other members of the group