Team:Hawaii/Notebook/2008-10-21

= Things we did today =

Insertion of lac-GFP device into BB-pRL1383a



 * Grace


 * Ran RE digests on gel
 * Bands were all incorrect size
 * Reinoculated TB+sp100 for plasmid prep tomorrow
 * PCR of J04430 from filter paper to verify part; used H/B primers

Team:Hawaii/Antibiotic test for BB-pRL1383a

 * Grace


 * Made LB+spvariable plates with 50 &mu;l 20X X-gal
 * Transformed 100 &mu;l DH5&alpha; with 2 &mu;l BB-pRL1383a (4 ng)
 * Plated 50 &mu;l cells + SOB on each LB+sp plate; plated 20 &mu;l untransformed cells + SOB on each control plate
 * Incubated at 37C for 2 days

Construction of GFP secretion device

 * Krystle
 * Gel purified overnight restriction digested nir+rbs
 * Loaded all of restriction digest into a 2% gel, which appeared blank after running for 1.5 hours
 * Re-PCR nir+rbs and slr+gfpf from plasmid prep
 * Restriction digest
 * nir+rbs with EcoRI and SpeI
 * slr+gfpf with XbaI and PstI

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"