Team:NYMU-Taipei/Project/Phosphate/Experiments/GEL Electrophoresis

Experiments to be verified by Electrophoresis

 * Gene cloning (PCR)
 * Digestion for vectors and inserts (Enzyme digestion)
 * Ligation
 * Transformation (Colony PCR)

PCR for new parts

 * PST operon
 * PST left
 * PST right
 * PPK (ppk mutant = PPK 1 + PPK 2)
 * PPK 1: 09/06 ready but lost; 09/11 OK
 * PPK 2: 09/17 ready

PCR for assembly

 * Basic Parts
 * B0032 (RBS, done)
 * R0010 (pLac promoter, done)
 * R0040 (pTet promoter, done)
 * P0440 (TetR with RBS and D-term, done)
 * Composition Parts
 * existed combination
 * pLac + E0240 (from Tina)
 * pPhoB + E0240 (non-standard cutting sites: E-part-S-P from dodo)
 * new combination
 * pLac + P0440: ligation A (8+10+20 failed) and A'(10 failed)
 * pTet + E0240: ligation B (10 successful)
 * RBS + PPK: ligation C (10+20 failed)
 * RBS + PST: ligation D