Purdue/25 September 2008

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Performed ligation reaction with LacZ and SOS:
 * Mixture:
 * 1uL T4 ligase
 * 2uL T4 buffer
 * 5uL LacZ DNA
 * 12uL SOS DNA
 * Let sit for 40 minutes at room temperature (should have been 10, but had to wait for the water bath to heat up)
 * Heat inactivated enzyme for 20 min at 55C


 * Craig, involved in learning Matlab, forgot about the enzyme. The water bath was at 58C when he took the enzyme out at 2:45PM.  Heat inactivation for 1 hour, 15 minutes.

Edited by Janie Stine