Team:Hawaii/Notebook/2008-10-20

= Things we did today =

Large scale plasmid prep of BB-pRL1383a (blue)

 * Grace

PCR of J04430 (lac+rbs+GFP+tt+tt)

 * Grace


 * Will act as (-) control for GFP secretion in cyanos

Overnight RE digest

 * Grace


 * EcoRI and PstI digest of BBpRL1383a and J04430

Made LB+sp100 plates

 * Grace

Prepped for sequencing

 * Krystle
 * Sent out correct-sized product to CORE for sequencing
 * nir+rbs
 * slr+gfpf

Construction of GFP secretion device

 * Krystle
 * Restriction digested additional PCR product
 * nir+rbs with EcoRI and SpeI

= Discussion =

= Quote of the Day = "Garbage truck dripping E. coli... this has got to stop. - Roswell Mayor, http://www.youtube.com/watch?v=f7mEb-p4F44 (ok maybe we should start taking Amp + Kan + Sm + Sp before going to the bathroom)"