Alberta NINT/23 July 2008

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lab work (SD): Results from 22/07/08 transformation: LB+amp50    LB+kan35    LB+amp50+kan35 WM     >100         >100         1 SD     >100         >100        16 Proceeded with B-Gal Activity Assay when cell cultures grown in the 96-well plate from 21/07/08 showed sufficient cell growth after two days at 37 C. Prepared media (LB+antibiotics+IPTG+glucose/arabinose) and inoculated 1 ml of media with 20 ul of cell cultures from the 21/07/08 96-well plate. Incubated at 37 C overnight. Isolated DNA from overnights of K102011, K102034, K102035, and K102036, using 3 ml to pellet cells using QIAprep spin miniprep standard protocol (to obtain more DNA for these constructs).