Team:Mississippi State/YPD Plate

=Preparing YPD Plates=


 * 1) Prepare 20% Dextrose solution by weighing out 40g Dextrose. Pour into dry beaker.  Also get an autoclaved 500ml bottle.
 * 2) Add 50ml water to beaker, stirring with stirbar.
 * 3) When dissolved, pour contents into graduated cylinder.
 * 4) Add water until volume is 200ml.
 * 5) Pour contents into 500ml Bottle.
 * 6) Get another beaker.
 * 7) Add 2.5g yeast extract, 5g Peptone, and 200ml H2O.
 * 8) Stir using stirbar.
 * 9) When dissolved, pour contents into graduated cylinder.
 * 10) Add H2O until volume is 225ml.
 * 11) Weigh out 5g Agar, and pour into empty, autoclaved flask.
 * 12) Pour contents of graduated cylinder into flask, covering with foil.
 * 13) Take both Dextrose solution and Yeast/Peptone/Agar solution to autoclave: 20min, liquid exhaust.
 * 14) Go to fume hood on 3rd floor and turn on UV light to sterilize area.
 * 15) When autoclave finishes, immediately swirl Yeast/Peptone/Agar solution insuring everything is well dissolved.
 * 16) Go to fume hood on 3rd floor.
 * 17) Turn off UV light, and turn on normal light.
 * 18) Place Bottle and flask into hood.
 * 19) Using sterile graduated cylinder, measure out 25ml 20% Dextrose solution.
 * 20) Add solution to Yeast/Peptone/Agar solution to get 250ml YPD media.
 * 21) Allow to cool to 55C in waterbath.
 * 22) Pour plates (7).