Team:Mississippi State/17 July 2008


 * 1) Extract plasmid using P1,P2,P3 buffers
 * 2) Prepare and run 1% agarose gel
 * 3) Check O.D. for samples: BKM#1 and BKM#6
 * 4) Make sequencing mix(x2): 4ul TRR, 1ul Template, 1ul Sp6(#1) 1ul T7(#2), 2ul 5xBuffer, 12ul ddH2O
 * 5) Run PCR: 96C, 2min; 96C, 10sec; 50C 5sec; 60C 4min;