Team:NTU-Singapore/Notebook/27 July 2008



  

=Sunday 27 July= {|border="1" style="background-color:#ffffcc;" cellpadding="20" Darius
 * Digested Lysis and terminator for an 1 and a half hour.
 * did a variation of lysis and terminator between inserts and vectors.
 * Gel extracted lysis insert(E & X)(sequential digestion, PCR purification)and lysis vector(E and S)( Non sequential). Gel extracted terminator insert(S & P) and terminator vector(E & X) (sequential).
 * Fast ligation.
 * Transformation