Team:Hawaii/Notebook/2008-07-31

= Things we did today =

Ligation of PCR products

 * Grace & Krystle


 * Ligated nir+rbs at 6:1 and 3:1 insert to vector concentrations (we didn't clean up RE digest so GP suggests higher vector concentration)
 * Ligated GFP+tt and GFPf+tt at 6:1 insert to vector concentrations (didn't have enough vector to do 3:1)
 * Ligated J33207+pRL1383a at 2:1 and 4:1 insert to vector concentrations

Transformed DB3.1 with ligated products

 * Grace

Ran a gel to check 7/30 gel

 * Krystle


 * Loaded 2 &mu;l PCR product into each well
 * PCR tubes likely mislabeled

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"