Team:Chiba/jk/β/weaks 3-4

31 August, 2008

 * Transformation
 * Competent Cells : XL10G
 * Plac+RBS+LasI
 * Plac+RBS+RhlI

Digestion
 * BBa_I9026(2007)
 * BBa_I9030(2006)
 * BBa_S03154(2007)
 * BBa_R0010(2007)


 * --->(1/9)Gel Check


 * (30/8)--->Mini prep


 * --->Digestion test
 * Plac+RBS+RhlI Sample No.2~5
 * BBa_T9002①、②

--->Gel Check

1 September, 2008

 * (31/8)--->Gel Check


 * --->zymo
 * vector-4(R0010)
 * -> 15μL


 * --->SAP
 * vectr-4(R0010)


 * --->Zymo
 * 1 -> 7μL
 * 2 -> 7
 * 3 -> 7
 * 4 -> 20

Transformation
 * Competent cells : BW ΔFliC 40μL
 * Plac+RBS+LasI : Sample No.L1~L4
 * Plac+RBS+RhlI : Sample No.R1~R4


 * --->(2/9)OD

2 September, 2008

 * (31/8)--->Gel Check


 * (1/9)--->Colony-PCR
 * Colony PCR of 8 colonies from ligation plates (1/9-(1),(2)) and one from control plate(BBa_F2620(2007)).




 * 95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃

--->Gel Check


 * --->(3/9)Mini prep


 * (1/9)--->OD測

Transformation
 * Competent cells : XL10G 30μL
 * C0161(2007)
 * C0161(2006)
 * C0261(2007)
 * C0261(2006)


 * --->(4/9)miniprep

3 September, 2008

 * (2/9)--->Mini prep


 * --->Gel Check


 * --->Digestion test
 * R1,R3~R7 (Plac+RBS+RhlI+LVA)
 * C1,C2,C4~C8 (Plac+RBS+CinI+LVA)


 * --->Gel Check

(2/9)--->Phenotype-test : mix
 * 8h
 * V
 * Spindown
 * V
 * the intensity GFP
 * V
 * the intensity GFP
 * V
 * the intensity GFP

4 September, 2008

 * --->Gel Check

13 September, 2008

 * Gel Check
 * BBa_C0161(2007)
 * BBa_J04500(2007)
 * BBa_R0010(2007)

--->[Team:Chiba/protocol/digestion|Digestion]]
 * BBa_C0161(2007)
 * BBa_J04500(2007)
 * BBa_R0010(2007)