Template:Team:UC Berkeley/Notebook/BX construction

pBca1256-K112000
PCR bx1/bx2 on Bjh1301		(688 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI) Product is pBca1256-K112000

bx1		Forward Biobricking of {T4 Holin!} ccagtGAATTCatgAGATCTatggcagcacctagaatatc bx2		Reverse Biobricking of {T4 Holin!} cgttaGGATCCttatttagcccttcctaatattctggccgcc

pBca1256-K112001
PCR bx3/bx2 on Bjh1301		(685 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI) Product is pBca1256-K112001

bx3		Forward biobricking of {} ccagtGAATTCatgAGATCTatggcagcacctagaatatc bx4		Reverse biobricking of {T4 Holin>} cgttaGGATCCtttagcccttcctaatattctggcc

pBca1256-K112003
PCR bx3/bx4 on Bjh1301		(682 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI) Product is pBca1256-K112003

bx3		Forward Biobricking of {} ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc bx4		Reverse Biobricking of {} cgttaGGATCCtttagcccttcctaatattctggcc

pBca1256-K112004
PCR bx5/bx2 on Bjh1301		(692 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI) Product is pBca1256-K112004

bx5		Forward Biobricking of {a~T4 Holin!} ccagtGAATTCatgAGATCTgtctatggcagcacctagaatatc bx2		Reverse Biobricking of {a~T4 Holin!} cgttaGGATCCttatttagcccttcctaatattctggccgcc

pBca1256-K112005
PCR bx6/bx2 on Bjh1301		(704 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI) Product is pBca1256-K112005

bx6		Forward Biobricking of {rbs.T4 Holin!} ccagtGAATTCatgAGATCTcttaaaaggagggtctatggcagcacctagaatatc bx2		Reverse Biobricking of {rbs.T4 Holin!} cgttaGGATCCttatttagcccttcctaatattctggccgcc

pBca1256-K112006
PCR bx7/bx8 on Bjh1303		(325 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI) Product is pBca1256-K112006

bx7		Forward biobricking of {T4 Antiholin!} ccagtGAATTCatgAGATCTatggccttaaaagcaacagcac bx8		Reverse biobricking of {T4 Antiholin!} cgttaGGATCCtcattcagtctccaatttaatgttc

pBca1256-K112007
PCR bx9/bx8 on Bjh1303		(322 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI) Product is pBca1256-K112007

bx9		Forward biobricking of {} ccagtGAATTCatgAGATCTatggccttaaaagcaacagcac bx10	Reverse biobricking of {T4 Antiholin>} cgttaGGATCCttcagtctccaatttaatgttc

pBca1256-K112009
PCR bx9/bx10 on Bjh1303		(319 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI) Product is pBca1256-K112009

bx9		Forward biobricking of {} ccagtGAATTCatgAGATCTgccttaaaagcaacagcac bx10	Reverse biobricking of {} cgttaGGATCCttcagtctccaatttaatgttc

pBca1256-K112010
PCR bx11/bx8 on Bjh1303		(329 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI) Product is pBca1256-K112010

bx11	Forward biobricking of {a~T4 Antiholin!} ccagtGAATTCatgAGATCTctttatggccttaaaagcaacagc bx8		Reverse biobricking of {a~T4 Antiholin!} cgttaGGATCCtcattcagtctccaatttaatgttc

pBca1256-K112011
PCR bx12/bx8 on Bjh1303		(339 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI) Product is pBca1256-K112011

bx12	Forward biobricking of {rbs.T4 Antiholin!} ccagtGAATTCatgAGATCTgataggaggcctttatggccttaaaagcaacagc bx8		Reverse biobricking of {rbs.T4 Antiholin!} cgttaGGATCCtcattcagtctccaatttaatgttc

pBca1256-K112012
PCR bx13/bx14 on Bjh1302		(526 bp, EcoRI/BamHI/DpnI) Sub into pBca1256				(EcoRI/BamHI) Product is pBca1256-K112012

bx13     Forward biobricking of {T4 Endolysin!} ccagtGAATTCatgAGATCTatgaatatatttgaaatgttacg bx14     Reverse biobricking of {T4 Endolysin!} cgttaGGATCCttatagatttttatacgcgtcccaagtgcc

pBca1256-K112013
PCR bx15/bx14 on Bjh1302		(523 bp, EcoRI/BamHI/DpnI) Sub into pBca1256				(EcoRI/BamHI) Product is pBca1256-K112013

bx15     Forward biobricking of {} ccagtGAATTCatgAGATCTatgaatatatttgaaatgttacg bx16     Reverse biobricking of {T4 Endolysin>} cgttaGGATCCtagatttttatacgcgtcccaagtgcc

pBca1256-K112015
PCR bx15/bx16 on Bjh1302		(520 bp, EcoRI/BamHI/DpnI) Sub into pBca1256				(EcoRI/BamHI) Product is pBca1256-K112015

bx15     Forward biobricking of {} ccagtGAATTCatgAGATCTaatatatttgaaatgttacgtatagatg bx16     Reverse biobricking of {} cgttaGGATCCtagatttttatacgcgtcccaagtgcc

pBca1256-K112016
PCR bx17/bx16 on Bjh1302		(530 bp, EcoRI/BamHI/DpnI) Sub into pBca1256				(EcoRI/BamHI) Product is pBca1256-K112016

bx17     Forward biobricking of {a~T4 Endolysin!} ccagtGAATTCatgAGATCTtattatgaatatatttgaaatgttacgtatagatg bx16     Reverse biobricking of {a~T4 Endolysin!} cgttaGGATCCtagatttttatacgcgtcccaagtgcc

pBca1256-K112017
PCR bx18/bx16 on Bjh1302		(542 bp, EcoRI/BamHI/DpnI) Sub into pBca1256				(EcoRI/BamHI) Product is pBca1256-K112017

bx18     Forward biobricking of {rbs.T4 Endolysin!} ccagtGAATTCatgAGATCTatacttaggaggtattatgaatatatttgaaatgttac bx16     Reverse biobricking of {rbs.T4 Endolysin!} cgttaGGATCCtagatttttatacgcgtcccaagtgcc

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