Team:Edinburgh/SU1

= SU1 =

Amino Acid Sequence
MAQQLPCVSSPRPlLAvPAGRWRAGvRGRPNvAGlGRGRlSLHAAAARPv AEAvQAEEDDDDDDEEvAEERFAlGGACRvLAGMPAPLGATALRGGVNFA VYSSGASAASlCLFAPGDLKADRvTEEvPLDPlLNRTGNvWHvFIHGDQl HGMLYGYRFDGvFAPERGQYYDvSNVvvDPYAKAvVSRGEYGvPAPGGSC WPQMAGMIPLPYNKFDWQGDLPLGYHQKDLVIYEMHlRGFTKHNSSKTKH PGTYIGAvSKLDHLKELGvNCIELMPCHEFNELEYFSSSSKMNFWGYSTI NFFSPMARYSSSGIRDSGCGAINEFKAFVREAHKRGIEvIMDVVFNHTAE GNEKGPILSFRGIDNSTYYMLAPKGEFYNYSGCGNTFNCNHPVVREFIvD ClRYWVTEMHVDGFRFDLASIlTRGCSLWDPVNvYGSPMEGDMITTGTPL VAPPLIDMISNDPILGNVKLIAEAWDAGGLYQVGQFPHWNVWSEWNGKYR DTvRQFIKGTDGFAGAFAECLCGSPQLYQAGGRKPWHSINFVCAHDGFTl ADlVTYNSKYNlSNGEDNRDGENHNLSWNCGEEGEFASlSVRRLRKRQMR NFFVCLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDHYVNYFRWDKKEEQS SDlYRFCRLMTKFRKECESLGLEDFPTSERlKWHGHQPGKPDWSEASRFV AFTMKDETKGEIYvAFNTSHLPvVVGLPERSGFRWEPvvDTGKEAPYDFL TDGLPDRAVTVYQFSHFLNSNLYPMLSYSSIILVlRPDV*

DNA Sequence
atggcgcagcagctcccctgcgtctcgtcgccgcgcccgctgctcgccgt 50

accgcgtcgtcgaggggacgcagagcagcggcgcgggcgacgagcggca

gcccgcgggccggtggcgcgccggcgtgcggggccggcccaatgtggcgg 100

cgggcgcccggccaccgcgcggccgcacgccccggccgggttacaccgcc

gactggggcgggggcggctgtctctccacgccgccgccgcgcggcccgtg 150

ctgaccccgcccccgccgacagagaggtgcggcggcggcgcgccgggcac

gccgaggcggtgcaggcggaggaggacgacgacgacgacgacgaggaggt 200

cggctccgccacgtccgcctcctcctgctgctgctgctgctgctcctcca

ggccgaggagaggttcgcgctgggcggcgcgtgccgggtgctcgcgggaa 250

ccggctcctctccaagcgcgacccgccgcgcacggcccacgagcgccctt

tgcccgcgccgctcggcgccaccgcgctccgcggcggtgtcaacttcgcc 300

acgggcgcggcgagccgcggtggcgcgaggcgccgccacagttgaagcgg

gtctactccagcggtgcctccgccgcgtcgctgtgcctcttcgctcccgg 350

cagatgaggtcgccacggaggcggcgcagcgacacggagaagcgagggcc

cgacctcaaggcggatagggtgaccgaggaggtgcccctcgatcccctgc 400

gctggagttccgcctatcccactggctcctccacggggagctaggggacg

tcaaccgaacgggaaacgtgtggcacgtgttcatccacggggaccagctg 450

agttggcttgccctttgcacaccgtgcacaagtaggtgcccctggtcgac

cacggcatgctctacggatacaggttcgatggcgtgttcgcccctgagcg 500

gtgccgtacgagatgcctatgtccaagctaccgcacaagcggggactcgc

cggacagtactacgatgtgtccaacgttgtggtggatccatacgctaagg 550

gcctgtcatgatgctacacaggttgcaacaccacctaggtatgcgattcc

cagtggtaagccgaggtgaatatggtgtgcctgcgcctggtggtagttgt 600

gtcaccattcggctccacttataccacacggacgcggaccaccatcaaca

tggcctcaaatggctggtatgatccctcttccctataataagtttgattg 650

accggagtttaccgaccatactagggagaagggatattattcaaactaac

gcaaggtgacctaccccttgggtaccatcagaaggaccttgtcatatatg 700

cgttccactggatggggaacccatggtagtcttcctggaacagtatatac

aaatgcatttgcgtggattcacaaagcacaactcaagcaagacaaaacac 750

tttacgtaaacgcacctaagtgtttcgtgttgagttcgttctgttttgtg

ccaggaacttacattggtgctgtgtcaaagcttgaccatctaaaggaact 800

ggtccttgaatgtaaccacgacacagtttcgaactggtagatttccttga

tggagtgaactgtatagagctaatgccctgccatgagttcaatgagctag 850

acctcacttgacatatctcgattacgggacggtactcaagttactcgatc

agtacttcagctcctcttcgaagatgaacttctggggatattccacaata 900

tcatgaagtcgaggagaagcttctacttgaagacccctataaggtgttat

aattttttctcaccaatggcaagatattcttcaagtggcataagagactc 950

ttaaaaaagagtggttaccgttctataagaagttcaccgtattctctgag

tggatgtggtgccataaatgaatttaaagcttttgtaagggaggcccaca 1000

acctacaccacggtatttacttaaatttcgaaaacattccctccgggtgt

aacggggaattgaggtgatcatggatgttgtcttcaatcatacagctgaa 1050

ttgccccttaactccactagtacctacaacagaagttagtatgtcgactt

ggtaatgagaaaggcccaatattatcctttagggggatagataatagtac 1100

ccattactctttccgggttataataggaaatccccctatctattatcatg

atactacatgcttgcacctaagggagagttttataattattctggttgtg 1150

tatgatgtacgaacgtggattccctctcaaaatattaataagaccaacac

gaaataccttcaattgtaatcatcctgtagtccgtgaatttatagtggat 1200

ctttatggaagttaacattagtaggacatcaggcacttaaatatcaccta

tgcttgagatactgggtaacagaaatgcatgttgatggttttcgttttga 1250

acgaactctatgacccattgtctttacgtacaactaccaaaagcaaaact

ccttgcatctatactgaccagaggatgcagtctatgggatccagttaatg 1300

ggaacgtagatatgactggtctcctacgtcagataccctaggtcaattac

tgtatggaagtccaatggaaggtgacatgattacgacagggacacctctt 1350

acataccttcaggttaccttccactgtactaatgctgtccctgtggagaa

gttgccccaccacttattgacatgattagcaatgacccaattcttggaaa 1400

caacggggtggtgaataactgtactaatcgttactgggttaagaaccttt

tgtcaagctcattgctgaagcatgggatgcaggaggtctctatcaagttg 1450

acagttcgagtaacgacttcgtaccctacgtcctccagagatagttcaac

gtcagtttcctcactggaacgtttggtcagagtggaatggaaagtatcgc 1500

cagtcaaaggagtgaccttgcaaaccagtctcaccttacctttcatagcg

gataccgtgcgtcagttcatcaaaggcacagatggatttgctggtgcttt 1550

ctatggcacgcagtcaagtagtttccgtgtctacctaaacgaccacgaaa

tgctgaatgcctatgtggaagtccacagttataccaggcaggggggagga 1600

acgacttacggatacaccttcaggtgtcaatatggtccgtcccccctcct

agccttggcacagtatcaactttgtatgtgcacacgatggatttacactg 1650

tcggaaccgtgtcatagttgaaacatacacgtgtgctacctaaatgtgac

gctgatttggtcacatacaatagcaagtacaacttgtcaaatggtgagga 1700

cgactaaaccagtgtatgttatcgttcatgttgaacagtttaccactcct

caacagagatggggaaaatcataatcttagctggaattgtggggaggaag 1750

gttgtctctaccccttttagtattagaatcgaccttaacacccctccttc

gagaatttgcaagtctgtcagtccgaagattaaggaagaggcaaatgcgc 1800

ctcttaaacgttcagacagtcaggcttctaattccttctccgtttacgcg

aatttctttgtttgtcttatggtttctcagggagttccaatgttctacat 1850

ttaaagaaacaaacagaataccaaagagtccctcaaggttacaagatgta

gggcgatgaatatggtcacacaaagggagggaacaacaatacgtactgcc 1900

cccgctacttataccagtgtgtttccctcccttgttgttatgcatgacgg

atgaccattatgtcaactatttccgttgggataagaaggaagaacaatcc 1950

tactggtaatacagttgataaaggcaaccctattcttccttcttgttagg

tctgatttgtacagattctgccgtctcatgaccaaattccgcaaggaatg 2000

agactaaacatgtctaagacggcagagtactggtttaaggcgttccttac

tgaatctcttggccttgaggacttcccgacttcagaacggttgaaatggc 2050

acttagagaaccggaactcctgaagggctgaagtcttgccaactttaccg

acggtcatcagcccgggaagcctgactggtcagaggcaagccgattcgtt 2100

tgccagtagtcgggcccttcggactgaccagtctccgttcggctaagcaa

gccttcaccatgaaggacgaaaccaaaggcgagatctacgtggccttcaa 2150

cggaagtggtacttcctgctttggtttccgctctagatgcaccggaagtt

caccagtcaccttccggtggttgtcgggcttccagagcgctctgggttcc 2200

gtggtcagtggaaggccaccaacagcccgaaggtctcgcgagacccaagg

gatgggagccggtggtggacaccggcaaggaggcaccatatgacttcctc 2250

ctaccctcggccaccacctgtggccgttcctccgtggtatactgaaggag

accgatggcctaccagatcgtgctgtcaccgtctaccagttctctcattt 2300

tggctaccggatggtctagcacgacagtggcagatggtcaagagagtaaa

cctcaactccaatctctatcctatgctcagctactcctccatcatccttg 2350

ggagttgaggttagagataggatacgagtcgatgaggaggtagtaggaac

tattgcgccctgatgtctga 2370

ataacgcgggactacagact

No forbidden restriction sites.

Primers
Forward: gat gaattc gcggccgc t tctag atg gcg cag ctc ccc tg

Reverse: gct actagt a tta tta gac atc agg gcg caa tac