Imperial College/15 August 2008

=15 August 2008=

Cloning

 * Plates form yestreday's transformations checked.
 * J31005, B0015, J04650 and I13401 transformants grew overnight but no growth was registered for the C0012 transformant nor the controls.


 * PCR primers for XylR, LacI and AmyE integration sequences designed and sent for synthesis

B.subtilis

 * The transformation protocol 2 | (click link here for the protocol) was performed yesterday. The transformed cells have not yet grown on the LB agar plates containing 100ug/ul streptinomycin. We will leave over the weekend to grow further.