Team:KULeuven/Model/Reset

Position in the system
The Pulse Generator-subsystem is directly linked to the Filter.

When the filter indicates that the input is zero (there is no desease), the system will (ideally) produce no lactonase. As soon as the output of the filter is one, the subsystem will produce a pulse of lactonase which will be high enough to 'remove' all HSL present in the system and in that way reset the timer.

ODE's
 NOT AVAILABLE

Parameters
All references for parameters can be found below, in the new section.

Problem
The idea of a pulsgenerator as reset mechanism doesn't meet the black-box requirements for the following reasons:


 * it takes too long before the proposed system generates a pulse-like event
 * the pulse itself is too long
 * a constant lactonase production sequence generates enough lactonase to reset the timer

Position in the system
The Constant Lactonase Production-system is directly linked to the Filter.

When the filter indicates that the input is zero (there is no desease), the system will (ideally) produce no lactonase. As soon as the output of the filter is one, the system starts producing lactonase and remains doing this untill the light goes off again. In this way all the HSL-molecules that are present will be 'removed' and the timer is reset.

Describing the system
see also: Project:Reset



ODE's
  

Simulation
During the first 50000 seconds, the input signal TetR (no aTc) is held constant at 5E-5 s-1. This results in a background signal of lactonase which will convert a small part of the HSL into hydroxy acid. From 50000 till 80000 seconds, the input signal is at its maximum value: 0.0125 s-1 (aTc added). This results in an increase of lactonase which converts almost every HSL molecule into the hydroxy acid. The timer is set to 0. After a delay of approximately 20000 seconds, the amount of HSL starts to increase again: the clock is ticking. A shorter pulse of TetR (1000 seconds) only partially resets the timer: not all the HSL is converted into hydroxy acid. The graph has amounts (number of molecules in the cell) plotted vs time, measured in seconds.