Team:Warsaw/Calendar-Main/26 September 2008

Mutagenesis of protein A Paweł

Results of mutageneses:  ADelL+KpnI and ADelP primers mutagenesis:  pACYClac+OmpA-&Delta;A-omega: 5 colonies pACYClac+OmpA-&Delta;A-alpha: 10 colonies pACYClac+OmpA-omega-&Delta;A</a>: 3 colonies</li> </ul> AMutL+KpnI and AMutP mutagenesis:</li> <ul> pACYClac+OmpA-&Delta;A-omega</a>: 20 colonies</li> pACYClac+Ompa-&Delta;A-alpha</a>: 12 colonies</li> pACYClac+OmpA-omega-&Delta;A</a>: 17 colonies</li> </ul> </ul> Each colony cultured overnight in LB + kanamycin for further analyses. </ol>

Preparation of &Delta;A (BBa_K103003)</a>

Piotr <ol>Isolation</a> of plasmid from culture inoculated on previous day.</li> Control digest</a> of isolated plasmid with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.</li></ol>