File:Fig 4-cloningstrategy colicinreceivers small.png

Cloning strategy of the LuxR-colicin-receiver parts (E1: BBa_K150009, E9 long: BBa_K150011, E9 short: BBa_K150013) First of all the LuxR receiver part (BBa_T9002) was amplified by PCR. The product, containing a Ptet promoter, the luxR gene followed by the PluxR promoter and a ribosome binding site was cloned into pSB1A2 vector. This vector was extracted from BBa_T9002. In a second cloning step the different colicin operons, amplified from pColE1 or pColE9-J were cloned behind the PLuxR promoter.