Team:Chiba/Calendar-Home/26 August 2008

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25 August 2008 <|> 27 August 2008

Team:Input
no work

Team:Communication

 * (8/25)--->Gel Check

--->Gel Extract


 * --->Zymo Clean


 * eluted with following amount of elution buffer
 * 5μl
 * 5μl
 * 27μl


 * --->Gel Check


 * --->SAP


 * --->Zymo Clean
 * Sample No.3 -->9μl


 * --->Gel Check


 * --->Ligation


 * --->Transformation
 * insert(1)BBa_C0178
 * insert(2)BBa_C0170
 * vector(3)BBa_J04500
 * insert(1)BBa_C0178+vector(3)BBa_J04500
 * insert(2)BBa_C0170+vector(3)BBa_J04500

Team:Output
Gel Check
 * BBa_F2620(1)
 * BBa_F2620(2)
 * BBa_J63001(3)

Digestion
 * vector:BBa_F2620(1)


 * insert:BBa_J52008(1)
 * BBa_E2030(2)

-->37°C　3hour -->SAP -->37°C 30min -->65°C 15min -->Zymo Clean

Ligation


 * vector:BBa_F2620 insert:BBa_J52008(1)
 * vector:BBa_F2620 insert:BBa_E2030(2)
 * Negative control:BBa_F2620(3)

-->RT 2hour

-->Transformation(XL10G)

-->37°C over night

-->colony PCR

-->Mini prep