Team:Chiba/Calendar-Home/25 August 2008

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24 August 2008 <|> 26 August 2008

Team:Input
The following glycerol Stock was made.

BBa_J22136(insert check OK)

Picked the colony and incubate with 2mL of LB-Ampicillin Medium for 12 hours at 37 degrees. Took 180&mu;l from culture,and mixed with 880&mu;lLB-Amp Medium.(OD=0.485)

Team:Communication

 * (24/8)--->Digestion
 * Double Digestion:BBa_J04500(2007)
 * Double Digesiton:BBa_R0010(2007)
 * Single Digestion:BBa_R0010(2007)
 * BBa_R0010(2007)


 * --->Gel Check

--->Digestion (Double Digestion)
 * BBa_C0178
 * BBa_C0170
 * BBa_J04500(2007)

Team:Output
PCR
 * BBa_E2030(1)
 * BBa_J33202(2)
 * BL21(3)
 * BBa_J52008(4)
 * BBa_I712019(5)

-->Gel Check

Mini prep
 * BBa_E2030
 * BBa_F2620

Transformation PCR
 * BBa_E2030
 * PUC19
 * pGFPUV
 * pGEX Venus YFP
 * Membren Venus YFP
 * BBa_J63001(1)