Team:Mississippi State/12 August 2008


 * redigest Lip and pPIC6 with EcoRI and NotI
 * Prepare ligation mix
 * prepare PCR mix to make more of the gene stock
 * prepare and run 1% agarose gel
 * purify PCR product
 * digest pcr w/ notI and ecoRI
 * cut out gel and purify w/ qiagen kit
 * make overnight culture (xlI-blue)
 * prepare ligation mix of digested Lip and pPIC6