Team:NTU-Singapore/Notebook/26 July 2008



   =Saturday 26 July= {|border="1" style="background-color:#ffffcc;" cellpadding="20" However, unsuccessful gel showed a band between 100bp and 200bp. Correct size of Lysis terminator should be 300bp.
 * Darius
 * Did a gel run for Lysis terminator.
 * Did a gel run for Lysis terminator.
 * miniprep another 12 tubes of LsrA standard vector innoculated yesterday.
 * digestion of the plasmid extracted.
 * Gel run check

LsrA is successful. band at 140bp. Though abnormality similar to Lysis, a double band is observed. 110bp and 150bp respectively, very close to each other( estimation of values)