Team:ESBS-Strasbourg/6 August 2008

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WetLab
Katja
 * PCR confirms the identy of the Gal4O, LacIO, LexAO, cIO, tetO BioBricks (Which are in the pSB1A2 backbone and in the Silver-standard)
 * Miniprep of the overnight cultures
 * Digestion with SpeI and PstI of the LacIO, LexAO, cIO and the tetO vectors -> back vectors
 * Ethanolic precipitation to get ride of the small fragments

Paul

-Digestion E+S of c1/lacI/lexA/tetR/EYFP/adh1-prom -> get insert -Digestion X+P adh1-ter -> get insert -Digestion E+X linker/Kozak -> get vector -Digestion S+P NLS -> get vector

Assembly we want to perform: c1+linker lacI+linker lexA+linker tetR+linker NLS+adh1term EYFP+linker adh1P+kozak