Template:Team:UC Berkeley/Notebook/AL construction

pBca1256-K112300
PCR al0001/al0002 on pSB3C6-Bjh1501    (508bp, EcoRI/BamHI/DpnI) Sub into pBca1256                     (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112300 --- al0001	Forward Biobricking of lambda lysozyme with start and stop codons CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg al0002	Reverse Biobricking of lambda lysozyme with start and stop codons CGttaGGATCCtcatacatcaatctctctgaccgttccgcc

pBca1256-K112301
PCR al0003/al0002 on pSB3C6-Bjh1501    (505bp, EcoRI/BamHI/DpnI) Sub into pBca1256                     (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112301 --- al0003	Forward Biobricking of Lambda Lysozyme with stop codon and no start codon CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg al0002	Reverse Biobricking of Lambda Lysozyme with stop codon and no start codon CGttaGGATCCtcatacatcaatctctctgaccgttccgcc

pBca1256-K112302
PCR al0001/al0004 on pSB3C6-Bjh1501    (505bp, EcoRI/BamHI/DpnI) Sub into pBca1256                     (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112302 --- al0001	Forward Biobricking of lambda lysozyme with start codon and no stop codon CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg al0004	Reverse Biobricking of lambda lysozyme with start codon and no stop codon CGttaGGATCCtacatcaatctctctgaccgttccgc

pBca1256-K112303
PCR al0003/al0004 on pSB3C6-Bjh1501    (502bp, EcoRI/BamHI/DpnI) Sub into pBca1256                     (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112303 --- al0003	Forward Biobricking of lambda lysozyme with no start/stop codon CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg al0004	Reverse Biobricking of lambda lysozyme with no start/stop codon CGttaGGATCCtacatcaatctctctgaccgttccgc

pBca1256-K112304
PCR al0005/al0002 on pSB3C6-Bjh1501    (512bp, EcoRI/BamHI/DpnI) Sub into pBca1256                     (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112304 --- al0005	Forward Biobricking of lambda lysozyme with spacer CCATAGAATTCatgAGATCTgaagatggtagaaatcaataatcaacgtaaggcgttcctcg al0002	Reverse Biobricking of lambda lysozyme with spacer CGttaGGATCCtcatacatcaatctctctgaccgttccgcc

pBca1256-K112305
PCR al0006/al0002 on pSB3C6-Bjh1501    (527bp, EcoRI/BamHI/DpnI) Sub into pBca1256                     (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112305 --- al0006	Forward Biobricking of lambda lysozyme with rbs site CCATAGAATTCatgAGATCTaaaaaagccggagtagaagatgg al0002	Reverse Biobricking of lambda lysozyme with rbs site CGttaGGATCCtcatacatcaatctctctgaccgttccgcc

pBca1256-K112306
PCR al0007/al0008 on pSB3C6-Bjh1502    (349bp, EcoRI/BamHI/DpnI) Sub into pBca1256                     (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112306 --- al0007	Forward Biobricking of lambda holin with start and stop codons CCATAGAATTCatgAGATCTatgccagaaaaacatgacctgttgg al0008	Reverse Biobricking of lambda holin with start and stop codons CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112307
PCR al0009/al0008 on pSB3C6-Bjh1502    (346bp, EcoRI/BamHI/DpnI) Sub into pBca1256                     (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112307 --- al0009	Forward Biobricking of lambda holin with stop codon and no start codon CCATAGAATTCatgAGATCTccagaaaaacatgacctgttggcc al0008	Reverse Biobricking of lambda holin with stop codon and no start codon CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112308
PCR al0007/al0010 on pSB3C6-Bjh1502    (346bp, EcoRI/BamHI/DpnI) Sub into pBca1256                     (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112308 --- al0007	Forward Biobricking of lambda holin with start and no stop codon CCATAGAATTCatgAGATCTatgccagaaaaacatgacctgttgg al0010	Reverse Biobricking of lambda holin wtih start and no stop codon CGttaGGATCCttgatttctaccatcttctactccggc

pBca1256-K112309
PCR al0009/al0010 on pSB3C6-Bjh1502    (343bp, EcoRI/BamHI/DpnI) Sub into pBca1256                     (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112309 --- al0009	Forward Biobricking of lambda holin with no start and stop codon CCATAGAATTCatgAGATCTccagaaaaacatgacctgttggcc al0010	Reverse Biobricking of lambda holin with no start and stop codon CGttaGGATCCttgatttctaccatcttctactccggc

pBca1256-K112310
PCR al0011/al0008 on pSB3C6-Bjh1502    (353bp, EcoRI/BamHI/DpnI) Sub into pBca1256                     (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112310 --- al0011	Forward Biobricking of lambda holin with spacer CCATAGAATTCatgAGATCTgaagatgccagaaaaacatgacctg al0008	Reverse Biobricking of lambda holin with spacer CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112311
PCR al0012/al0008 on pSB3C6-Bjh1502    (369bp, EcoRI/BamHI/DpnI) Sub into pBca1256                     (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112311 --- al0012	Forward Biobricking of lambda holin with rbs site CCATAGAATTCatgAGATCTattgggggtaagacCtgaag al0008	Reverse Biobricking of lambda holin with rbs site CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112312
PCR al0013/al0008 on pSB3C6-Bjh1503    (355bp, EcoRI/BamHI/DpnI) Sub into pBca1256                     (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112312 --- al0013	Forward Biobricking of lambda antiholin with start and stop codons CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc al0008	Reverse Biobricking of lambda antiholin with start and stop codons CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112313
PCR al0014/al0008 on pSB3C6-Bjh1503    (352bp, EcoRI/BamHI/DpnI) Sub into pBca1256                     (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112313 --- al0014	Forward Biobricking of lambda antiholin with stop codon and no start codon CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg al0008	Reverse Biobricking of lambda antiholin with stop codon and no start codon CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112314
PCR al0013/al0010 on pSB3C6-Bjh1503    (352bp, EcoRI/BamHI/DpnI) Sub into pBca1256                     (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112314 --- al0013	Forward Biobricking of lambda antiholin with start codon and no stop codon CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc al0010	Reverse Biobricking of lambda antiholin with start codon and no stop codon CGttaGGATCCttgatttctaccatcttctactccggc

pBca1256-K112315
PCR al0014/al0010 on pSB3C6-Bjh1503    (349bp, EcoRI/BamHI/DpnI) Sub into pBca1256                     (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112315 --- al0014	Forward Biobricking of lambda antiholin with no start and stop codons CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg al0010	Reverse Biobricking of lambda antiholin with no start and stop codons CGttaGGATCCttgatttctaccatcttctactccggc

pBca1256-K112316
PCR al0015/al0008 on pSB3C6-Bjh1503    (359bp, EcoRI/BamHI/DpnI) Sub into pBca1256                     (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112316 --- al0015	Forward Biobricking of lambda antiholin with spacer CCATAGAATTCatgAGATCTagacatgaagCtgccagaaaaacatgacc al0008	Reverse Biobricking of lambda antiholin with spacer CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112317
PCR al0016/al0008 on pSB3C6-Bjh1503    (375bp, EcoRI/BamHI/DpnI) Sub into pBca1256                     (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112317 --- al0016	Forward Biobricking of lambda antiholin with rbs site CCATAGAATTCatgAGATCTccccttattgggggtaagacatg al0008	Reverse Biobricking of lambda antiholin with rbs site CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112318
PCR al0017/al0018 on MG1655    (467bp, EcoRI/BamHI/DpnI) Sub into pBca1256                     (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112318 --- al0017	Forward Biobricking of bolA promoter CCATAGAATTCatgAGATCTtgctgtggcagtgtaatcgtc al0018	Reverse Biobricking of bolA promoter CGttaGGATCCcttctatccgctcacgtatc

pBca1256-K112319
PCR al0019/al0024 on MG1655 (404bp, gp = A) PCR al0023/al0023 on MG1655 (86bp, gp=B) --- PCR al0019/al0020 on A+B    (465bp, EcoRI/BamHI/DpnI) Sub into pBca1256                     (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112319 --- al0019	Forward Biobricking of ftsQ promoter CCATAGAATTCatgAGATCTttgattgaaaaatggctaagtgggccgg al0020	Reverse Biobricking of ftsQ promoter CGttaGGATCCcctcttcttcgctgtttcgc al0023 Forward Removing of EcoRI ggtagtacgaattGtggaactggcg al0024 Reverse Removing of EcoRI cgccagttccaCaattcgtactacc

pBca1256-K112320
PCR al0021/al0022 on MG1655    (804bp, EcoRI/BamHI/DpnI) Sub into pBca1256                     (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112320 --- al0021	Forward Biobricking of ftsAZ promoter CCATAGAATTCatgAGATCTaacgacagtgttggtgagcg al0022	Reverse Biobricking of ftsAZ promoter CGttaGGATCCgtaccaatctccagtcctactaccag

pBjh1601AC-K112321
PCR JH088F/JHO88R on Salmonella L2. (435bp, EcoRI/BamHI/DpnI) Sub into pBjh1601AC                     (EcoRI/BamHI, 3195+910, L) Product is pBjh1601AC-K112321 --- JH088F  Forward biobricking of {H-NS!} cgataGAATTCatgAGATCTATGAGCGAAGCACTTAAAATTC JH088R  Reverse biobricking of {H-NS!} cgttaGGATCCTTATTGCTTGATCAGGAAATCG

pBjh1601CK-K112322
PCR JH089F/JH089R on Salmonella L2. (379 bp, EcoRI/BamHI/DpnI) Sub into pBjh1601CK            (EcoRI/BamHI, 3134+910, L) Product is pBjh1601CK-K112322 --- JH089F  Forward biobricking of {Pdps} cgataGAATTCatgAGATCTaacgattcgacgcgaaccgc JH089R  Reverse biobricking of {Pdps} cgttaGGATCCaatctcatatcctcttgatg

pBCa1601AC-K112323
PCR ca998/JH090R on pBjh1601AC-K112321-2   (445 bp, EcoRI/BamHI) Sub into pBjh1601AC                     (EcoRI/BamHI, 3195+910, L) Product is pBjh1601AC-K112323 --- ca998  Forward biobricking of {H-NS!} Salmonella gtatcacgaggcagaatttcag JH090R  Reverse biobricking of {H-NS!} Salmonella cgttaGGATCCTTATTcCTTGATCAGG

pBCa1601CK-K112324
Digest (BgIII methylated) K112902 CA, (BamHI methylated) K112709 AK     (BamHI/BgIII/XHoI) Product is pBjh1601CK-K112324

pBCa1601CK-K112325
Digest (BgIII methylated) K112704 CA, (BamHI methylated) K112709 AK     (BamHI/BgIII/XHoI) Product is pBjh1601CK-K112325

pBCa1601AC-K112327
Digest (BgIII methylated) K112230 AK, (BamHI methylated) K112203 KC     (BamHI/BgIII/XHoI) Product is pBjh1601AC-K112327

pBCa1601AC-K112328
Digest (BgIII methylated) K112227 AK, (BamHI methylated) K112102 KC     (BamHI/BgIII/XHoI) Product is pBjh1601AC-K112328

pBCa1601AC-K112329
Digest (BgIII methylated) K112230 AK, (BamHI methylated) K112113 CK      (BamHI/BgIII/XHoI) Product is pBjh1601AC-K112329

pBCa1601AC-K112330
Digest K112614 AK (BgIII, XhoI) Digest K112105 KC(BamHI, XhoI) Product is pBjh1601AC-K112330

pBCa1601AC-K112331
Digest K112615 AK (BgIII, XhoI) Digest K112108 KC(BamHI, XhoI) Product is pBjh1601AC-K112331

pBCa1601AC-K112332
Digest (BgIII methylated) K112615 AK, (BamHI methylated) K112216 KC      (BamHI/BgIII/XHoI) Product is pBjh1601AC-K112332

pBCa1601AC-K112333
Digest (BgIII methylated) K112614 AK, (BamHI methylated) K112222 KC     (BamHI/BgIII/XHoI) Product is pBjh1601AC-K112333

pBca1601AK-K112334
Digest (BgIII methylated) K112230 AC, (BamHI methylated) K112324 CK    (BamHI/BgIII/XHoI) Product is pBjh1601AK-K112334

pBca1601AK-K112335
Digest (BgIII methylated) K112227 AC, (BamHI methylated) K112324 CK     (BamHI/BgIII/XHoI) Product is pBjh1601AK-K112335

pBca1601AK-K112336
Digest (BgIII methylated) K112614 AC	 (BamHI/XhoI) Digest (BamHI methylated) K112324 CK     (BgIII/XHoI) Product is pBjh1601AK-K112336

pBca1601AK-K112337
Digest (BgIII methylated) K112615 AC, (BamHI methylated) K112324 CK     (BamHI/BgIII/XHoI) Product is pBjh1601AK-K112337

pBca1601AK-K112339
Digest (BgIII methylated) K112327 AC    (BamHI/XhoI) Digest (BamHI methylated) K112324 CK    (BgIII/XHoI) Product is pBjh1601AK-K112339

pBca1601AK-K112340
Digest (BgIII methylated) K112328 AC, (BamHI methylated) K112324 CK     (BamHI/BgIII/XHoI) Product is pBjh1601AK-K112340

pBca1601AK-K112341
Digest (BgIII methylated) K112329 AC, (BamHI methylated) K112324 CK     (BamHI/BgIII/XHoI) Product is pBjh1601AK-K112341

pBca1601AK-K112342
Digest (BgIII methylated) K112330 AC, (BamHI methylated) K112324 CK     (BamHI/BgIII/XHoI) Product is pBjh1601AK-K112342

pBca1601AK-K11234
Digest (BgIII methylated) K112332 AC, (BamHI methylated) K112324 CK    (BamHI/BgIII/XHoI) Product is pBjh1601AK-K112344

pBca1601AK-K112348
Digest (BgIII methylated) K112328 AC, (BamHI methylated) K112325 CK    (BamHI/BgIII/XHoI) Product is pBjh1601AK-K112348

pBca1601AK-K112349
Digest (BgIII methylated) K112329 AC, (BamHI methylated) K112325 CK    (BamHI/BgIII/XHoI) Product is pBjh1601AK-K112349

pBca1601AK-K112350
Digest (BgIII methylated) K112330 AC	 (BamHI/XhoI) Digest (BamHI methylated) K112325 CK    (BgIII/XHoI) Product is pBjh1601AK-K112350

pBca1601AK-K112351
Digest (BgIII methylated) K112331 AC	 (BamHI/XhoI) Digest (BamHI methylated) K112325 CK    (BgIII/XHoI) Product is pBjh1601AK-K112351

pBca1601CK-K112354
Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112334 AK    (BamHI/BgIII/XHoI) Product is pBjh1601CK-K112354

pBca1601CK-K112355
Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112335 AK    (BamHI/BgIII/XHoI) Product is pBjh1601CK-K112354

pBca1601CK-K112356
Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112336 AK    (BamHI/BgIII/XHoI) Product is pBjh1601CK-K112354

pBca1601CK-K112357
Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112337 AK    (BamHI/BgIII/XHoI) Product is pBjh1601CK-K112354

pBca1601CK-K112360
Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112340 AK    (BamHI/BgIII/XHoI) Product is pBjh1601CK-K112360

pBca1601CK-K112361
Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112341 AK    (BamHI/BgIII/XHoI) Product is pBjh1601CK-K112361

pBca1601CK-K112362
Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112342 AK    (BamHI/BgIII/XHoI) Product is pBjh1601CK-K112362

pBca1601CK-K112363
Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112343 AK    (BamHI/BgIII/XHoI) Product is pBjh1601CK-K112363

pBca1601CK-K112368
Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112348 AK    (BamHI/BgIII/XHoI) Product is pBjh1601CK-K112368

pBca1601CK-K112369
Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112349 AK    (BamHI/BgIII/XHoI) Product is pBjh1601CK-K112369

pBca1601CK-K112370
Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112350 AK    (BamHI/BgIII/XHoI) Product is pBjh1601CK-K112370

pBca1601CK-K112371
Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112351 AK    (BamHI/BgIII/XHoI) Product is pBjh1601CK-K112371

pBca1601CK-K112372
Digest (BgIII methylated) K112121 CA, (BamHI methylated) K112709 AK    (BamHI/BgIII/XHoI) Product is pBjh1601CK-K112372

pBca1601CK-K112373
Digest (BgIII methylated) BBa_K112232 CA, (BamHI methylated) K112709 AK    (BamHI/BgIII/XHoI) Product is pBjh1601CK-K112373