Wiki/Team:Warsaw/igem notebook.htm





Protocols 

 Purification of His_Z_alpha and His_Z_omega Purification of His_A_alpha

Testing various hunter/prey combinations</li>

Plasmid DNA isolation</a></li> DNA isolation from agarose gel</a></li> DNA purification after enzymatic reaction</a></li> Genomic DNA isolation</a></li> Preparation of chemocompetent bacteria</a></li> Preparation of electrocompetent bacteria</a></li> Chemotransformation</a></li> Electrotransformation</a></li>

<li><a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a></li>

<li><a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#blunting">Blunting</a></li> <li><a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#concentrations">Standard concentrations of antibiotics and other supplements </a></li> <li><a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#rif">Rifampicin test </a></li> <li><a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">Removing 5' phosphate groups from DNA ends </a></li> <li><a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#taxi">TAXI protocol (Tet+Ap 30+X-Gal+IPTG) </a></li> <li><a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#bca">Protein concentration measurement (BCA method) </a></li> <li><a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">DNA digest </a></li> <li><a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR </a></li> <li><a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcl">Polymerase Chain Ligation (PCL) </a></li>

</ol>

<a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/primers">Primers</a> <a href="http://2008.igem.org/Wiki/Team:Warsaw/vectors">Vectors</a> <h3 align="left" OnClick="switchMenu('strains','menu_item')" onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Strains <div name="menu_item" id="strains" style="display:none;"> Rosetta(DE3)pLysS</a> F- ompT hsdSB(RB- mB-) gal dcm &lambda;(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR) TOP10 (Invitrogen)</a> F- mcrA &Delta;(mrr-hsdRMS-mcrBC) &phi;80lacZ&Delta;M15 &Delta;lacX74 nupG recA1 araD139 &Delta;(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 &lambda;- <a name="mutD5">NR9458 (<a href="http://www.ibb.waw.pl/">Institute of Biochemistry and Biophysics</a> collection; in notebook called mutD5)</a> ara thi &Delta; pro lac mutD5 zaf-13 ::Tn10 GM2163</a> ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2 <h3 align="left" OnClick="switchMenu('javascript','menu_item')" onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">JavaScript tools developed to make work with iGEM notebook more comfortable <div name="menu_item" id="javascript" style="display: none"> <ol> <li><a href="http://2008.igem.org/Team:Warsaw/Calendar-Main/NoAtribution">Author attribution analyzer</a></li> <li><a href="http://2008.igem.org/Team:Warsaw/JSTest2">Inretactive Notebook topics list</a></li> </ol> <img src="http://2008.igem.org/wiki/images/5/52/Spacer.gif" width="1" height="530" border="0" alt="" />

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