Imperial College/9 October 2008

=9 October 2008=

Wetlab

 * The ligations of various parts were transformed and single colony PCR was carried out to check for correct ligation. The following parts that were ligated were:
 * 3'AmyE + GFP-T
 * 3'AmyE + RFP-T
 * 6L(LipA-Elastin)
 * The pSB primers were used on the following cycle:
 * 1 cycle - 95oC for 30 sec
 * 30 cycle - 95oC for 30 sec, 60oC for 45 sec, 72oC for 1 min


 * All ligations appear to have worked and now midi preps will be prepared.