Team:Warsaw/Calendar-Main/23 June 2008

Blue/white and rifampicin test #2 Piotr Analysis of lacZ sequence from pZC320: There is no mutation in lacZ gene. Why are the colonies white? Reason of white phenotype unknown; this might be chromosomal mutation, transcription supression or something else).

Preparation of constructs: OmpA_alpha and OmpA_omega Michał K. Inoculation other separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha  and pACYC177+OmpA_omega (liquid LB + kanamycin).

Preparation of alpha-A and omega-A fusions Michał L. We have successfully amplified Omega-linker (Fig. 1), but were unable to obtain the other. two PCRs were repeated. New annealing temp.: 48&deg;C. Mg2+ concentration elevated to 4 mM.  Fig. 1.PCR products. Lane 1 - lack of product for A-linker; lane 3 - product for Omega-linker.