Waterloo/23 June 2008

08/06/23 Monday Morning [x] Mini prep cultures from Sunday (2007 and 2008 registry)           10:00 - 12:30 - 2007 done, but need to redo 2008 miniprep on Wednesday [x] need to re-innoculate 2008 (samples were mixed up) - Keep it at 4C tomrrow for wed to mini prep with 2007 registry parts.

[x] need more solution 1 [x] update 1-2-3 mini prep protocol

[x] Diagnostic digest of mini preps (12:45 - 1:30) and gel (1.5 hr) - Look at DNA concentrations for previous ligations to trouble shoot low transformation efficiency of the ones that we have enough vol (11 out 14) all have concentrations > 30ng/uL

[x] elute the rest of the 2007 registry parts from the registry plate. - One transformant didn't work, will be repeated