Judging/Variance/UC Berkeley

Request
Dear iGEM Judges,

I'm writing on behalf of the 2008 UC Berkeley iGEM Team to request permission to submit parts in the BBb standard of Biobricks to the registry for this year's Jamboree.

For the second year in a row, the UC Berkeley iGEM team is using the BBb standard of Biobricks, where parts are flanked by BglII and BamHI sites rather than XbaI and SpeI sites. Over 1,000 parts have been developed in this standard and the BBb standard has been described and discussed in the BioBricks community. More information about this standard is described at http://openwetware.org/wiki/Arking:JCATutorialIntro1 In the spirit of iGEM, we hope to share these parts with the community, and we are constructing them using biobricks, but with a variation on the original version. Given the strengths the BBb Biobrick standards, we hope you will accept our constructs for this year's competition.

Thank you for your consideration,

Madhvi Venkatesh (Anderson Lab)

UC Berkeley iGEM Team

Response
Madhvi,

Thanks for your email. Terrific / request approved!

We would be grateful if you could please refer to the assembly standard that you are using as "BioBricks beta option 1" or "BBb1" for short.

Several "BBb" standards have been proposed, developed, or are in use, and we are working to provide clarity across the world-wide BioBricks community. More information is online here: http://openwetware.org/wiki/The_BioBricks_Foundation:Standards/Technical/Formats

Also, please coordinate with the Registry staff regarding how you will be preparing any submitted DNA so that they can prepare to handle any necessary BBb1 plasmids, et cetera.

All best, Drew