Team:Hawaii/Notebook/2008-07- 8

= Things we did today =

Completed oligonucleotide experiment

 * Grace


 * Ran RE digested ligations on 4% gel. Too low DNA concentration so no ligated products were seen.
 * Redid ligation and RE digest. Loaded 20 &mu;l in 4% gel.
 * RE digested C0012.
 * Gel extraction of C0012 vector and ligated products.
 * Ligated C0012 vector with pnir, slr2016, and pilA.

Biobrick extraction

 * Krystle


 * Extracted BBa_E0240 and BBa_I14032 using commercial grade DH5&alpha; super competent cells.

PCR of pRL1383a parts

 * Margaret

* Ran a gel of the rep and mob region.

= Discussion =

= Quote of the Day =