Team:Hawaii/Effect of Water Motion on Clumping of Liquid Cultures

Hypothesis
Liquid Cultures of Synechocystis PCC6803 clump when incubated in Erlenmeyer Flasks and does not when cultured in 'box' culture flasks: Is this due to the motion of the media in different containers, the surface area of the culture, is it reproducible?

Methods
1. Question 1: Does the clump go back to homogeneous when placed in square culture flask? 1. Test: Took 0.5mL of a clump and placed it in 2 square culture bottles. (1 of the bottles was fresh BG-11 media, the other was the old stock to which buffer was added today) 2. Compare to: Took 0.5mL of a homogeneous liquid culture and placed it in 2 square culture bottles. (1 of the bottles was fresh BG-11 media, the other 1 was the old stock to which buffer was added today) 2. Question 2:Will previously homogeneous culture go to clump? 1. Homogeneous liquid culture placed in BG-11 media in an Erlenmeyer flask 1:10, Vfinal=50 mL        2. Homogeneous liquid culture placed in BG-11 media in an Culture flask 1:10, Vfinal=50 mL

Results

 * The homogeneous culture is now clumping. Clumps are reverting to homogeneous

Conclusions
This is a good way to get out cultures to clump if that is ever needed.

Discussion
"History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"
 * If our action is not recorded, we are doomed to either repeat the error, or fail to replicate the success.