Team:University of Ottawa/2 July 2008

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 Home  Welcome  The Team</a>  Who We Are</a>  Advisors</a></li> Undergrads</a></li> </ul> </li> What We've Done</a></li> Where We're From</a></li> Contact Us</a></li> </ul> </li> The Project</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Project_Overview">Overview</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#The_Template">Template</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#The_Design">Design</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#Applications">Application</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Project#References">References</a></li> </ul> </li> <li><a href="http://partsregistry.org/Part:BBa_K149001:Design">BioBricks</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Modeling">Modeling</a></li> <li><a class="MenuBarItemSubmenu" href="http://2008.igem.org/Team:University_of_Ottawa/Wet_Lab">Wet Lab</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Lab_Protocols">Lab Protocols</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/WetWare">WetWare</a></li> </ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Notebook">Notebook</a></li> <li><a class="MenuBarItemSubmenu" href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors">Sponsors</a> <ul> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Acedemic_Sponsors">Academic</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Research_Sponsors">Research</a></li> <li><a href="http://2008.igem.org/Team:University_of_Ottawa/Sponsors#Corporate_Sponsors">Corporate</a></li> </ul> </li>

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Today in the Lab
Matt
 * Ligation
 * <li> I am using three different samples of 2:1, 3:1, 4:1 molar ratio of insert to vector being used for ligation of PTP2 to pSSA42.
 * <li> A gel confirmation was run however nothing appeared on the gel due to a very low DNA concentration of the PTP2.
 * <li> We decided it was better just to try and integrate into competent cells and incubate overnight for tomorrow morning.
 * Transformation
 * <li> The competent cells were transformed with the ligation product of all three samples and left overnight.The receptor component Atcre was also integrated into competent cells.
 * Dehydrogenase component
 * <li> The dehydrogenase was received in the mail, I streaked the Ecoli containing the component to grow overnight in the incubator. Tomorrow we will inoculate for mini prep the following day.

Dan
 * PCR of pSSRE constucts
 * <li> Turned out to be a disaster. Had nonspecific binding and >4 bands. We would be better of going back to the original gel extraction products.
 * Digestion
 * <li> Now I am using the original gel extraction products (1,2,3,4) and