Team:University of Ottawa/5 August 2008

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Chris

 * <li> Inoculation control was contaminated; will reperform later today.

Digestion of Construct OA, OB and T Amp Product
 * <li> In order to increase the scope of our microwave digestion experiment, I added the following samples for comparison purposes.


 * <li> Digestion was performed according to previous protocol, including denaturation at 80&deg;C and addition of ligase+ ATP.
 * <li> Ran all products on a 1% gel; however, did not run digestion products as a control and had to redo the experiment.

Ligation Microwave Optimisation <li>'''1. Digestion'''


 * <li> Incubated at 37&deg;C for 15 minutes, followed by 20 minutes at 80&deg;C to denature the enzymes

2. Ligation
 * <li> Added 1&mu;L each ligase and ATP to the digestion samples before subjecting them to the following conditions:


 * <li> All samples stored at 4&deg;C following ligation
 * <li> Also: inoculated 5 samples each of Matt's 3:1 and 6:1 PTP2/PSSA42 construct for minipreparation tomorrow.