Team:Hawaii/Notebook/2008-10- 7

= Things we did today =

Construction of secretion device (cont.)

 * Grace


 * Ran RE digests on gel
 * J33207 did not cut
 * Extracted bands from gel
 * Ligated:
 * nir + rbs+gfp+tt#1
 * plac + rbs+gfp+tt#1
 * nir+rbs + slr1+GFPf
 * plac+rbs + slr1+GFPf
 * nir+rbs + pilA+GFPf+tt
 * plac+rbs + pilA+GFPf+tt
 * pSB1A3 to self (control)
 * Used 5 &mu;l ligation mixture to transform DH5&alpha; cells

Drylab Work

 * Grace, Krystle, Margaret


 * Revised project abstract

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"