User:University of Washington/9 September 2008

LuxR from AraC and TetR(Faifan)
-Ran gel of the PCR products x14 colonies, got no DNA in all lanes-probably because cells weren't lysed or there weren't any cells at all from the beginning(resuspension wasn't good enough to disperse the cells in dH2O)

-Resuspended 28 colonies into 20 ul dH2O (14 from yesterday's resuspension, 14 from plate), boiled at 100 degree Celsius for 16 mins.

-Did Colony PCR again
 * mix 12.6ul dH2O + 2 Buffer + 0.4ul 10mM-dNTP + 0.8ul 50mM-MgCl2 + 1ul 10mM-VF2 + 1ul 10mM-VR + 0.2ul Taq + 2ul Template
 * Thermocycling
 * 1 : 95 : 1 min
 * 30: 95 : 30 s
 * __: 53 : 30 s
 * __: 72 : 1 min
 * 1 : 72 : 7 mins
 * 1 : 4 : forever

-Grew overnight of MG1655Z1 from stock in Tsy

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