Team:Hawaii/Notebook/2008-07-18

= Things we did today =

PCR of pRL1383a Parts

 * Margaret


 * for details concerning the running conditions, please look here.
 * Ran a 0.8%gel at 95V. [[Image:PCR_7_18_08.jpg|right|thumb|300px|Please refer to the annotated picture.]]

Extraction of Biobricks and Transformation in DB3.1

 * Margaret
 * streaked plates on Amp100 plates


 * for details please look here.

Sequencing

 * Grace


 * Redid PCR reactions for GFP fusion, BB-pRL1383a, B0015, J04430, R0010
 * Gel OK this time (95V for 50 min; ladder resolved, loading dye didn't run funny)
 * Incorrect bands still for B0015, J04430, R0010; Krystle will redo the plasmid preps for these
 * Correct bands for GFP fusion and BB-pRL1383a. BB-pRL1383a band faint -- why?
 * Treated PCR products w/ ExoSAP
 * Determined DNA concentrations using nanodrop spectrometer (measured twice)


 * Sent 22 samples to CORE Hawaii for sequencing

Media Making
Margaret, Krystle (thanks for the help!)
 * Made one sleeve of Amp100 plates.

Plasmid Prep of GFP fusion Brick, R0010, J3340

 * Krystle


 * did a large scale (75 ml) plasmid prep for the GFP fusion brick
 * Performed 3 mini preps each of the BioBrick parts

Project Description (Abstract)

 * Grace, Krystle, Margaret


 * Wrote description of project (abstract)

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"