Team:BCCS-Bristol/Protocols-Transformation with heat shock



Transformation with heat shock

 * 1) 	Thaw the chemical competent bacteria (50 µl E. coli DH5α) on ice
 * 2) 	Add the DNA by pipetting it directly in the middle of the bacteria solution und mix by stirring gently with the pipette tip
 * 3) 	Incubate 20 min on ice
 * 4) 	Heat shock: incubate 60 s at 42°C in a water bath
 * 5) 	Incubate 2 min on ice
 * 6) 	Add 250 µl SOC broth (stored in the freezer; thaw before and check for no contamination!)
 * 7) 	Incubate for 1 h at 37°C 225 rpm
 * 8) 	Plate 50 µl and 100 µl on two LB plates with antibiotics
 * 9) 	Incubate the plates at 37°C overnight. Store the remaining bacteria in the fridge.