Team:Hawaii/Notebook/2008-10-15

= Things we did today =

Construction of secretion device (cont.)

 * Grace


 * Transformed DH5&alpha;
 * Used 5 &mu;l of n/p + rgt ligation reaction
 * Used 1 &mu;l of J33207/pRL1383a ligation reaction
 * Plated 25 &mu;l, 50 &mu;l, 75 &mu;l, and 100 &mu;l plates in hopes of isolating single colonies


 * Krystle
 * PCR
 * nir+rbs
 * slr+gfpf
 * Restriction digest overnight
 * nir+rbs with EcoRI and SpeI
 * slr+gfpf with XbaI and PstI
 * pSB1A3 with EcoRI and SpeI

Ligation

 * Margaret


 * lac promoter/rbs(34) (E,S) + rep2 (X,P) + pSB1A3 (de-phosphorylated, E,P)
 * lac promoter/rbs(30)rep/oriV(6) (E,S) + lac promoter/rbs(34)aada/oriT(A) (X,P) +pSB1A3 (de-phosphorylated, E,P)
 * lac promoter/rbs(30)rep/oriV(6) + lac promoter/rbs(34)aada/oriT (A)
 * lac promoter/rbs(30)rep/oriV (6) (E,S) + lac promoter/rbs(34)aada/oriT(B) (X,P) +pSB1A3(de-phosphorylated, E,P)
 * oriV (E,S) + oriT (X,P) + pSB1A7 (E,P)
 * pSB1A7 self (did not de-phosphorylate) (E,P)

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"