Team:Hawaii/Notebook/2008-09-23

= Things we did today =

Plasmid prep

 * Grace


 * pSB1A3
 * rbs+GFPf+tt #1, 2
 * nir+rbs+GFP #1, 4, 7
 * nir+rbs+slr1+GFPf #2, 8
 * nir+rbs+pilA #18

Sequencing

 * Margaret




 * PCR of rep, ran a gel to verify size, and exo-sap. will send in tomorrow

PCR: pRL1383a MCS Replacement

 * Norman


 * x6 parallel MCS replacements fragment by PCR amplification. PCR out each MCS replacement insert with HindIII-VF2BB_fx._sb.1 and BamHI-VRBB_rx._sb.1
 * BBa_I52002 from pSB4A5 (Spring 2008 Plate 1022 1C)
 * BBa_I52001 from pSB4T5 (Spring 2008 Plate 1020 1A)
 * BBa_I52002 from BBa_I51020 (BBa_I51020 Base Vector from glycerol stock Inventory INV-Z80-R1-A3)
 * BBa_P1010 from pSB1A3 (pSB1A3 from glycerol stock Inventory INV-Z80-R1-A3)
 * BBa_P1010 from pSB1A7 (pSB1A7 from glycerol stock Inventory INV-Z80-R1-A3)
 * BBa_J33207 from pSB1A2 (BBa_J33207 harboring pSB1A2 from plasmid prep)
 * x2 (or x3) Controls
 * positive control: BBa_K125805 from pSB1A3 (BBa_K125805 harboring pSB1A3 from glycerol stock Inventory INV-Z80-R1-D4)
 * negative control: H2O primer set used on 1-7
 * negative control: H2O old Bam-VF2BB Hind-VRBB primers


 * Are you sure the controls had contaminated primers, that they weren't contaiminated during the pipetting process? There's only a single band in each old primer control lane (contaminants usually give more than one band). Also, the band is different between the first gel (~850bp?) and the second (~920bp).*Gracek 07:35, 25 September 2008 (UTC)

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"