PCR Purification

PCR Purification
PCR Purification allows for a buffer exchange and the ability to get rid of unwanted enzymes still surrounding the plasmid DNA.

Using QIAquick kit

1.	Add 5 volumes of Buffer PBI to 1 volume of sample.

2.	Pipette into a QIAquick spin column(max 770 µl) and centrifuge for 60 sec at 10,000g

3.	Discard flow-through.

4.	Wash: add 0.75ml Buffer PE(make sure that the buffer has ethanol added to it) to column and centrifuge for 1 min

5.	 Discard flow-through & centrifuge for 1 min

6.	 Place column into clean Eppendorf tube

7.	 Add 50ul Buffer EB or water to center of membrane

8.	 Let stand at RT for 3 min

9.	 Centrifuge for 5 min.

Measure the concentration using the UV spectrophotometer