Team:NTU-Singapore/Notebook/5 July 2008



  

=Saturday 5 July= {|border="1" style="background-color:#ffffcc;" cellpadding="20"
 * Among the plates collected, only the plate of LacI-RFP has colonies. Plates with E7-empty plasmid and LacI-GFP do not have colony.
 * Among the plates collected, only the plate of LacI-RFP has colonies. Plates with E7-empty plasmid and LacI-GFP do not have colony.

Lu Chao

 * minipreps for LacI-RBS 34.

Hung, Lu Chao

 * Nanodrop to determine concentrations and purities of LacI-RBS 34 plasmids, which show good results.
 * Prepare digestion mixtures for gel confirmation.

Darius

 * Gel electrophoresis to check if there are LacI-RBS 34 plasmids. Result: All have the 2000 kb band, the empty vector plasmid (expected). Besides that band:


 * There is 1 sample that only has 250 bp band, which is LacI-RBS 34. (Good!!)
 * There is 2 samples that have both 250 bp band and less than 50 bp band, which can be interpreted as RBS (due to self-ligation of RBS).
 * The rest only have the RBS band.