Alberta NINT/15 July 2008

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lab work (SD): Since the double-plasmid cell cultures failed to grow in LB+amp50+kan35 broth, perhaps the colonies grown on LB+amp50+kan35 plates were not selected for kan resistance (ie. kan was not present on             these plates). New LB+amp50+kan35 plates were made and the transformation was redone. K102022 (LB+amp50) K102032 (LB+amp50) K102032+K102011 (LB+amp50+kan35) K102032+K102034 (LB+amp50+kan35) K102032+K102035 (LB+amp50+kan35) K102032+K102036 (LB+amp50+kan35)

lab work (JD): We've decided to re-do the ligation another time from scratch: Created O/N from glycerol stocks of: pSB4A5 K102003 K102006