Newcastle University Wetlab/13 August 2008

Wednesday 13th August
- pGFPrrnB restricted with EcoRI + NheI
 * pGFPrrnB and pJWV021 were restricted using 10μl plasmid and 0.5μl each enzyme in 50μl total reaction volume:

- pJWV021 restricted with NheI then BglII in a 2 step reaction (the second step used 48μl purified plasmid in 100μl total reaction volume). These were stored overnight at -25˚C.


 * BBa_I746101 (from Plate 1018 Spot 7A) and BBa_I746107 (containing the agr 2-component system) were received as cell stocks from the iGEM Registry.