Template:Team:UC Berkeley/Notebook/Molly construction

Molly's Construction Files
Molly's Notebook

Construction of {xis!} Biobrick Part K112200 PCR mea001/mea002 on pAH57		(230 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112200

mea001	Forward Biobricking of {xis!}	ccataGAATTCatgAGATCTatgtacttgacacttcaggag mea002	Reverse Biobricking of {xis!}	cgttaGGATCCtcatgacttcgccttcttccc

Construction of {} Biobrick Part K112202 PCR mea001/mea004 on pAH57		(227 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112202

mea001	Forward Biobricking of {xis!}	ccataGAATTCatgAGATCTatgtacttgacacttcaggag mea004	Reverse Biobricking of {xis>}	cgttaGGATCCtgacttcgccttcttcccatttc

Construction of { } Biobrick Part K112203 PCR mea003/mea004 on pAH57		(224 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112203

mea003	Forward Biobricking of {}	cgttaGGATCCtgacttcgccttcttcccatttc

Construction of {a~xis!} Biobrick Part K112204 PCR mea017/mea002 on pAH57		(234 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112204

mea017	Forward Biobricking of {a~xis!}ccagtGAATTCatgAGATCTtgcgatgtacttgacacttcagg mea002	Reverse Biobricking of {xis!}	cgttaGGATCCtcatgacttcgccttcttccc

Construction of {rbs.xis!} Biobrick Part K112205 PCR mea018/mea002 on pAH57		(249 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112205

mea018	Forward Biobricking of {rbs.xis!}ccagtGAATTCatgAGATCTtaattgcggagactttgcgatg mea002	Reverse Biobricking of {xis!}	cgttaGGATCCtcatgacttcgccttcttccc

Construction of {int!} Biobrick Part K112207 PCR mea005/mea006 on pAH57		(1102 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112207

mea005	Forward Biobricking of {int!}	ccataGAATTCatgAGATCTatgggaagaaggcgaagtcatg mea006	Reverse Biobricking of {int!}	cgttaGGATCCttatttgatttcaattttgtcccactccc

Construction of {} Biobrick Part K112209 PCR mea005/mea008 on pAH57		(1099 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112209

mea005	Forward Biobricking of {int!}	ccataGAATTCatgAGATCTatgggaagaaggcgaagtcatg mea008	Reverse Biobricking of {int>}	cgttaGGATCCtttgatttcaattttgtcccactccc

Construction of { } Biobrick Part K112210 PCR mea007/mea008 on pAH57		(1096 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112210

mea007	Forward Biobricking of {}	cgttaGGATCCtttgatttcaattttgtcccactccc

Construction of {a~int!} Biobrick Part K112211 PCR mea019/mea006 on pAH57		(1106 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112211

mea019	Forward Biobricking of {a~int!}ccataGAATTCatgAGATCTagaaatgggaagaaggcgaag mea006	Reverse Biobricking of {int!}	cgttaGGATCCttatttgatttcaattttgtcccactccc

Construction of {rbs.int!} Biobrick Part K112212 PCR mea020/mea006 on pAH57		(1120 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112212

mea020	Forward Biobricking of {rbs.int!}ccataGAATTCatgAGATCTttttgaagaggatcagaaatggg mea006	Reverse Biobricking of {int!}	cgttaGGATCCttatttgatttcaattttgtcccactccc

Construction of {ihfA!} Biobrick Part K112213 PCR mea009/mea010 on MG1655		(331 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112213

mea009	Forward Biobricking of {ihfA!}ccataGAATTCatgAGATCTatggcgcttacaaaagctgaaatg mea010	Reverse Biobricking of {ihfA!}	cgttaGGATCCttactcgtctttgggcgaagcg

Construction of {} Biobrick Part K112215 PCR mea009/mea012 on MG1655		(328 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112215

mea009	Forward Biobricking of {ihfA!}ccataGAATTCatgAGATCTatggcgcttacaaaagctgaaatg mea012	Reverse Biobricking of {ihfA>}	cgttaGGATCCctcgtctttgggcgaagcg

Construction of {} Biobrick Part K112216 PCR mea011/mea012 on MG1655		(325 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112216

mea011	Forward Biobricking of {}	cgttaGGATCCctcgtctttgggcgaagcg

Construction of {a~ihfA!} Biobrick Part K112217 PCR mea021/mea010 on MG1655		(335 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112217

mea021	Forward Biobricking of {a~ihfA!}ccataGAATTCatgAGATCTacctatggcgcttacaaaagc mea010	Reverse Biobricking of {ihfA!}	cgttaGGATCCttactcgtctttgggcgaagcg

Construction of {rbs.ihfA!} Biobrick Part K112218 PCR mea022/mea010 on MG1655		(350 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112218

mea022	Forward Biobricking of {rbs.ihfA!}ccataGAATTCatgAGATCTtcattgagggattgaacctatggcgc mea010	Reverse Biobricking of {ihfA!}	cgttaGGATCCttactcgtctttgggcgaagcg

Construction of {ihfB!} Biobrick Part K112219 PCR mea013/mea014 on MG1655		(316 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112219

mea013	Forward Biobricking of {ihfB!} ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagacttgccaccc mea014	Reverse Biobricking of {ihfB!}	cgttaGGATCCttaaccgtaaatattggcgcgatcg

Construction of {} Biobrick Part K112221 PCR mea013/mea016 on MG1655		(313 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112221

mea013	Forward Biobricking of {ihfB!} ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagacttgccaccc mea016	Reverse Biobricking of {ihfB>}	cgttaGGATCCaccgtaaatattggcgcgatc

Construction of {} Biobrick Part K112222 PCR mea015/mea016 on MG1655		(310 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112222

mea015	Forward Biobricking of {}	cgttaGGATCCaccgtaaatattggcgcgatc

Construction of {a~ihfB!} Biobrick Part K112223 PCR mea023/mea014 on MG1655		(320 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112223

mea023	Forward Biobricking of {a~ihfB!}ccataGAATTCatgAGATCTaatcatgaccaagtcagaattgatag mea014	Reverse Biobricking of {ihfB!}	cgttaGGATCCttaaccgtaaatattggcgcgatcg

Construction of {rbs.ihfB!} Biobrick Part K112224 PCR mea024/mea014 on MG1655		(337 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112224

mea024	Forward Biobricking of {rbs.ihfB!}ccataGAATTCatgAGATCTctttaaggaaccggaggaatcatg mea014	Reverse Biobricking of {ihfB!}	cgttaGGATCCttaaccgtaaatattggcgcgatcg

Construction of {ompT>} Biobrick Part K112225 Wobble PCR mea025/mea026 		(97 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112225

mea025	Forward Biobricking of {ompT>}	cgataGAATTCatgAGATCTatgcgggcgaaactcctaggaatagtcctgacaacccctatcg mea026	Reverse Biobricking of {ompT>}	cgttaGGATCCcgtcgacgcaaaagagctgatcgcgataggggttgtcaggactattc

Construction of {a~ompT} Biobrick Part K112226 Wobble PCR mea027/mea026 		(101 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112226

mea027	Forward Biobricking of {a~ompT}	cgataGAATTCatgAGATCTacatatgcgggcgaaactcctaggaatagtcctgacaacccctatc mea026	Reverse Biobricking of {ompT>}	cgttaGGATCCcgtcgacgcaaaagagctgatcgcgataggggttgtcaggactattc

Construction of {rbs.ompT} Biobrick Part K112227 Wobble PCR mea028/mea026 		(114 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112227

mea028	Forward Biobricking of {rbs.ompT}cgataGAATTCatgAGATCTaagaaggagatatacatatgcgggcgaaactcctaggaatagtcctgacaacccc mea026	Reverse Biobricking of {ompT>}	cgttaGGATCCcgtcgacgcaaaagagctgatcgcgataggggttgtcaggactattc

Construction of {pelB>} Biobrick Part K112228 Wobble PCR mea029/mea030 		(97 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112228

mea029	Forward Biobricking of {pelB>}	cgataGAATTCatgAGATCTatgaaatacctgctgccgaccgctgctgctggtctgctgctcc mea030	Reverse Biobricking of {pelB>}	cgttaGGATCCggccatcgccggctgggcagcgaggagcagcagaccagcagcagcgg

Construction of {a~pelB} Biobrick Part K112229 Wobble PCR mea031/mea030 		(101 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112229

mea031	Forward Biobricking of {a~pelB}	cgataGAATTCatgAGATCTacatatgaaatacctgctgccgaccgctgctgctggtctgctg mea030	Reverse Biobricking of {pelB>}	cgttaGGATCCggccatcgccggctgggcagcgaggagcagcagaccagcagcagcgg

Construction of {rbs.pelB} Biobrick Part K112230 Wobble PCR mea032/mea030 		(116 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112230

mea032	Forward Biobricking of {rbs.pelB} cgataGAATTCatgAGATCTttaagaaggagatatacatatgaaatacctgctgccgaccgctgctgctggtctgctg mea030	Reverse Biobricking of {pelB>}	cgttaGGATCCggccatcgccggctgggcagcgaggagcagcagaccagcagcagcgg

Construction of {rbs.xis.int!} Biobrick Part K112234 PCR mea018/mea006 on pAH57 	       (1317 bp, EcoRI/BamHI/DpnI) Sub into pBca1256			(EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112234

mea018	Forward Biobricking of {rbs.xis!}ccagtGAATTCatgAGATCTtaattgcggagactttgcgatg mea006	Reverse Biobricking of {int!}	cgttaGGATCCttatttgatttcaattttgtcccactccc

Molly's Notebook