Waterloo/30 July 2008

08/07/30 Wed

[] p/s/i of POCI rfp and gfp - no growth observed - religate and retransform

[x] full digestion of c0052, c0051

[x] diagnostic digest of yesterday's miniprepped dna (c0052, c0051, J22106. b0025)

[x] check gel of T7 gene 5 (extracted from yesterday) and diagnostic dig [x] gel of full digestion

[x] gel extraction [] ligation of c0052', c0052', POCI rfp and gfp

[x] repeat PCR (28/07) of T7 gene 6

[x] promoter testing - plac rfp'' was restreaked and inoculated in liquid LB

[x] speed vac t7 gene 5 to 20 microlitre volume

[] if cloning of gene 6 worked, clone with pjet vector

[] gene 5 PCR, set up another PCR with different conditions/PCR program - shorter elongation time; spike mixes w/ polymerase partway through, etc..