Team:Warsaw/Calendar-Main/24 May 2008

Rifampicin test #2 Piotr Conclusion: AID seems to work!

Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7 Michał K.

  Isolation of hypothetical pMPM-T5 with transcriptional fusion from cultures.

 Isolation of hypothetical pMPM-T5 with translational fusion from cultures.</li>

 Control digest</a> of pMPM-T5 with transcriptional fusion</a>with EcoRI (EcoRI buffer).</li>

 Control digest</a> of pMPM-T5 with translational fusion</a> with BamHI (BamHI buffer).</li>

 Gel electrophoresis (still no proper colonies found).</li> </ol>