Team:Warsaw/Calendar-Main/25 July 2008

Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA Paweł  Isolation of plasmids from cultures inoculated on previous day. Control digest of isolated plasmids with NdeI and NotI. Two positives obtained (proper band: ~200 bp visible). One of positive plasmids transformed into TOP10 and plated on LB+amp, overnight, for further isolation of pET15b+Z+omega vector.</li> </ol>

Cloning of protein A DNA to OmpA constructs Michał K. <ol>Isolation of plasmids</a> from cultures inocluated on previous day (pACYC177+OmpA_A_omega</a>).</li> Control digest</a> of isolated plasmids with BamHI and SacI (BamHI buffer).</li> Gel electrophoresis - proper clone found. </li></ol>