Team:The University of Alberta/30 September 2008

can't tell much from the gel2 gel1. expected size 3000bp (2-9) and 2000bp(10-16). this is as expected gel2. expected size 4000bp (1-8) and 4200bp(10-16). result is as expected, but have some short band(contaminations?) they all seems good! have to sequence to make sure.
 * Tom

David
starting the bisdA and bisdB cassettes from scratch because i really, really, really want to see if this stuff will work.

i digested

the correct pTET vector complete with RBS site and 6xHis tag (pTET) with SphI+PstI+CIP treatment BisdA with XbaI+PstI BisdB with XbaI+PstI

i gel purified the products and set up the following ligations

pTET+BisdA pTET+BisdB