Virginia/19 October 2008

Overnight Results

 * A77(4) and A81 had colonies
 * A82 had no colonies
 * Butanolysis run: Conc of P3HB - 300 ul of 17.2ug of P3HB

Accomplished

 * Made overnight broth of A77(4) and A81
 * Two colonies each
 * Replated A82 from saved cells
 * Ligated A82(2) (1:1 ratio of molten low-melt gel volumes)
 * Gel of A79(2)-1,2 (X,P) looks good

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