Utah State/17 June 2008

We learned a good amount about how to use the Spectrofluorometer today by finding optimal Excitation and Emission wavelengths for the four fluorescent proteins we have transformed into E. coli. We found that RFP is best excited at 550 nm and best tested for emission at 603 nm, CPF at 425 and 480, GFP at 480 and 510, and YFP at 520 and 540.