Team:Chiba/jk/γ/time

Designs
In this plan, we induce cross-talk of quorum sensing by changing receiver protein.

Expetriments

 * sender
 * AHL sender(BBa_S03623)


 * reciever
 * LuxR
 * RhlR(BBa_K084004)
 * LasR(BBa_K084005)
 * CinR(BBa_K084006)


 * reporter
 * Plux-GFP(BBa_J37032)

Method

 * 1) Transformed Sender into E.coli strains(JW1908) and Receivers into E.coli strain(JW1908).
 * 2) Inoculated them independently in liquid media. Incubated at 37c&deg; 12h.
 * 3) Inoculated again at 37c&deg; upto about OD600=2.0
 * 4) Washed them.
 * 5) Mixed them (Sender:Receiver=1000&mu;l:1000&mu;l).
 * 6) Incubated at 30c&deg;.
 * 7) Measured intensity of green fluorescence at regular time intervals.