Utah State/9 July 2008

A restriction enzyme digest was performed with the three GFP constructs, the luciferase construct, and the GFP (needing IPTG). An electrophoresis gel was run. Colonies were prepared for testing of the GPF construct needing IPTG. A rogue colony that had the appearance of GFP under UV was also streaked on a plate containing IPTG and one without as a control. Liquid cultures were also prepared, with and without IPTG. The GFP needing IPTG had a reddish hue. Fluorescence testing will be performed tomorrow.