Team:Hawaii/Notebook/2008-08- 7

= Things we did today =

Plasmid prep

 * Krystle


 * Finished plasmid prep of BB-pRL1383a, nir, GFPf from yesterday

Cyanobacteria seed stocks

 * Krystle


 * Reinoculated liquid and solid media seed stocks

Verification of transformants

 * Grace


 * Colony PCR of transformants
 * Ran on EtBr stained 2.5% agarose gel at 95V for 75 min.
 * Looks like nir+rbs and I14032+rbs worked. YAY :o). Will prep for sequencing tomorrow.
 * GFP, GFPf, J33207 don't look like they went in. Will sequence to confirm anyway.

Plasmid Prep
Margaret
 * Re-did yesterday's plasmid prep of pSMC121 because I don't believe the band was big enough. I e-mailed Dr. Callahan to get the exact size, but I know, because it contains the omega interposon, that it is at least 2kb.

Extraction from Gel
Margaret


 * extracted 3 bands of 50ul reaction for rep and oriV first by cutting out using long wave UV for visualization.
 * Purification from gel with Qiagen Minelute gel purification kit.

PCR
Margaret


 * Large-scale PCR amplification of aadA (it was lost in a gel cutting mishap) and p1 lytic.

= Discussion =

= Quote of the Day = Gernot: "So would you eat the E. coli'' to save them if we lose the -80C in like a war?" Loren: "Well, I'll eat them, but you'll be collecting them."