Team:The University of Alberta/21 May 2008

Volunteers Scheduled for Today

5-8 AS 6-8 DL

Things to do: Mini-preps of O/N's Sequence gel purified fragments from yesterday Transform I0500 Digest J61003 with Xba/Pst to insert ORF's Digest minipreps with Xba/Pst to insert into J61003 Run gel of digests and excise correct fragments Ligate vector/inserts Transform ligation reactions Lab clean up

Jason :  Started investigating the Cry1 blue light detection pathway  Started to build the LUX operon plant biobrick  Went to Dougs awesome Modeling Course James : Checked all O/N's set up by SZ and WX - all grew except 023 WX. Will set up again tonight. Followed standard Qiagen miniprep protocol for all o/n's.

Winnie and Saima : Digested J61003 using Xba and Pst Digested minipreps of last year's genes using Xba and Pst Began sequencing the o/n minipreps

Chris : Got the results back from the thiolase gene sequencing. Chromatograms look good; unfortunately the sequence doesnt seem to be correct. I BLASTed the sequences and came up with Acetyl-CoA Acetyltransferase. Very odd indeed. EDIT: Seems thiolase is a synonym for Acetyl-CoA Acetyltransferase. The sequence still doesnt match the one from last year, though.

The Good and Bad news
The Good News Our system will work in E.coli The Bad News In 2004 Texas submitted the cyanbacterial equivalent of our system, so... Our biobricks would be unique but they would do the exact same thing. The Good News</U> I looked up a very promising pathway that detects blue light instead The Bad News</U> I can only find a summary of the paper... Here is the link to it http://www.arabidopsis.org/servlets/TairObject?type=publication&id=501724582

Lab Tip of the Day
When using the NanoVu to measure your DNA concentration, please ensure that you press the measure button and not the calibration button. Also, remember that the instructions for using the NanoVu are posted above the NanoVu. Please refer to these instructions in times of doubt instead of getting Chris to do it for you.

PS: Falafel does not a good pizza make.

For your Consideration
Sound Emitting Bacteria http://www.jstage.jst.go.jp/article/jgam/44/1/44_49/_article