Team:The University of Alberta/29 May 2008

Volunteers Scheduled for Today
130-4 NK 4-8 AH 5-9 KR 6-9 DL

On this Episode of "What we Did in the Lab Today"
Chris: I dunno if these are in the correct order but I don't think it really matters. As long as the enzymes are being produced and have the expected activity, things should be fine (correct me if I'm wrong!)
 * Got sequencing results on B0043 back. I aligned them with the sequences from last year to see what they were (just doing a Blast on the optimized sequences doesnt seem to work for some reason):
 * B0043-VF (the first gene) seems to be ß-Hydroxy Butyryl Co-A Dehydrogenase (1)
 * B0043+1 (the second gene) seems to be Enoyl-CoA Hydratase
 * B0043+2 (the third gene) seems to be Butyryl-CoA Dehydrogenase
 * B0043+3 (the fourth gene) seems to be Butyraldehyde Dehydrogenase
 * B0043+4 (the fourth gene) seems to be Butanol Dehydrogenase
 * B0043+5 (the fifth gene) aligned with nothing, which is good because there was no 5th gene in the operon
 * B0043-VR (sequencing from reverse) aligned with part of Butanol Dehydrogenase, which confirms that it is the last gene in the operon so far.

Jason
 * Researched the GAA promoter that we could used for our ERE.
 * Designed a Possible BioBrick
 * Learned how to run a protien gel

Tune in Later for the Exciting conclusion of "What we did in the lab today"