Team:University of Lethbridge/Notebook/Project4September

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Selina
Objective: Create glycerol stocks of the xylE plasmid for future use.

--- Set up transformation of xylE pVL1392 plasmid (50 pg) with 25 uL E. coli DH5a cells.

Followed manufacturer's (Invitrogen) 'high-yield' protocol.

Plated 100 uL on LB + Amp and incubated overnight at 37C.

Selina
Checked xylE transformation plates for colonies - success!

Inoculated one 5 mL LB + Amp culture tube and incubated at 37 C for 4.5 hours.

Christa, Selina
Created 3 glycerol stocks of E. coli DH5a xylE pVL1392 transformants and placed in -80C.