Alberta NINT/27 May 2008

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lab work (SD):

DNA digest of K102000 with SpeI + PstI. Incubated at 37 C for 1 hour. Run on 2% agarose gel. ~3 kbp fragment expected. Ligated K102000 with E0433 and K102000 with null_output.

lab work (JD):

Recovered Part BBA_R011 from iGEM package Transformed XL1-B cells and plated on LB+Amp100 plates. Created 2 O/Ns as well.

lab work (WM):

Prepare BBa_K102010 fragments for ligation. K102010 is AraC::PromBad::TA0In AraC::PromBad comes is in BBa_I0500/SpeI+PstI (5.6kb fragment, including plasmid) TA0In (200bp) was synthesized commercially and placed in a vector. Gel-purify BBa_I0500/SpeI+PstI and TA0In?XbaI+PstI fragments from 2% agarose gel.