Team:NTU-Singapore/Notebook/23 June 2008



  

=Monday 23 June= {|border="1" style="background-color:#ffffcc;" cellpadding="20"

Morning (9am-1230pm)

 * DNA extraction (using Miniprep kit) for 21 samples that Choon Kit inoculated on Sunday
 * Digestion of the above 21 plasmids by EcoRI and PstI, followed by Gel electrophoresis check after 2 hours incubation.
 * Digestion LacI promoter insert using SpeI and EcoRI sequentially with QiAgen PCR purification kit in between.
 * Digestion of RBS 0034 vector using XbaI and EcoRI sequentially and QIAgen PCR purification kit.

Chin Chong

 * Preapre LBA agar plates of Top10 and BL21 containing LacI-GFP and allowed to incubate overnight at 37 deg cel
 * Designed the Lysis gene (with Promoter and RBS) and LsrA gene and requested for Quotations from companies
 * Inoculate top10 and BL21 cells containing LacI-GFP and grow in two seperate tubes containing 5 ml of LBA
 * allowed to incubate overnight at 37 deg cel
 * Autoclaved MgSO4, CaCl2 and M9 salts