Team:Edinburgh/GBS1

= GBS1 =

For better alignment see .txt file

AMINO ACID SEQUENCE
MATvTASSNFvSRTSLFNNHGASSCSDVAQITLKGQSlTHCGLRSFNMvD

NLQRRSQAKPVSAKSSKRSSKVKTAGKIvCEKGMSvIFIGAEVGPWSKTG

GLGDVLGGLPPALAARGHRvMTICPRYDQYKDAWDTCVvVQIKVGDKVEN

VRFFHCYKRGVDRVFVDHPIFLAKVvGKTGSKIYGPITGVDYNDNQLRFS

llCQAALEAPQVlNlNSSKYFSGPYGEDVVFVANDWHTALLPCYLKSMYQ

SRGVYMNAKvVFCIHNIAYQGRFAFDDYSLLNlPISFKSSFDFMDGYEKP

VKGRKINWMKAAIlEAHRVLTVSPYYAQELISGVDRGvElHKYLRMKTVS

GIINGMDVQEWNPSTDKYIDIKYDITTVTDAKPlIKEALQAAVGLPvDRD

VPVIGFIGRlEEQKGSDILvEAISKFMGLNVQMVILGTGKKKMEAQILEL

EEKFPGKAVGvAKFNvPlAHMITAGADFIIVPSRFEPCGLIQlHAMRYGT

VPIvASTGGLvDTvKDGYTGFHIGRFNVKCEVvDPDDvIATAKAvTRAVA

VYGTSAMQEMVKNCMDQDFSWKGPARlWEKVLlSLNvAGSEAGTEGEEIA

PlAKENVATP*

DNA SEQUENCE
atggcaactgtgactgcttcttctaactttgtgtcaagaacttcactttt 50

taccgttgacactgacgaagaagattgaaacacagttcttgaagtgaaaa

caacaatcatggtgcttcttcatgctctgatgtcgctcagattaccttaa 100

gttgttagtaccacgaagaagtacgagactacagcgagtctaatggaatt

aaggccaatccttgactcattgtgggttaaggtcattcaacatggtggat 150

ttccggttaggaactgagtaacacccaattccagtaagttgtaccaccta

aaccttcagaggagatctcaagctaaacctgtttctgctaaatcctcaaa 200

ttggaagtctcctctagagttcgatttggacaaagacgatttaggagttt

gagatcttctaaagttaagactgctggtaagattgtgtgtgagaaaggaa 250

ctctagaagatttcaattctgacgaccattctaacacacactctttcctt

tgtctgtgatttttattggagctgaagttggtccatggagtaaaactggt 300

acagacactaaaaataacctcgacttcaaccaggtacctcattttgacca

ggtcttggtgatgttctcggtggtctacctccagctcttgctgctagagg 350

ccagaaccactacaagagccaccagatggaggtcgagaacgacgatctcc

ccaccgtgtgatgacaatttgtcctcggtatgaccaatataaagatgctt 400

ggtggcacactactgttaaacaggagccatactggttatatttctacgaa

gggacacttgtgttgtggttcagatcaaagttggggataaagttgagaat 450

ccctgtgaacacaacaccaagtctagtttcaacccctatttcaactctta

gttcgtttcttccattgctacaaacgaggagttgatcgtgtctttgttga 500

caagcaaagaaggtaacgatgtttgctcctcaactagcacagaaacaact

ccatccaatctttcttgctaaggttgtgggcaaaacaggatccaaaatct 550

ggtaggttagaaagaacgattccaacacccgttttgtcctaggttttaga

atggtcctataactggagtagactacaatgacaaccaactccggttcagt 600

taccaggatattgacctcatctgatgttactgttggttgaggccaagtca

ttgttgtgtcaggctgctcttgaggcaccacaggttctgaacctgaacag 650

aacaacacagtccgacgagaactccgtggtgtccaagacttggacttgtc

cagcaagtacttctctggaccatatggtgaagatgtagtctttgttgcca 700

gtcgttcatgaagagacctggtataccacttctacatcagaaacaacggt

atgactggcacactgctctacttccatgttacctcaaatctatgtatcaa 750

tactgaccgtgtgacgagatgaaggtacaatggagtttagatacatagtt

tcccgcggagtctacatgaatgcaaaggtggtcttctgcattcacaacat 800

agggcgcctcagatgtacttacgtttccaccagaagacgtaagtgttgta

agcctaccagggaagatttgcctttgatgactattcccttctcaacttgc 850

tcggatggtcccttctaaacggaaactactgataagggaagagttgaacg

ccatcagctttaaaagttctttcgacttcatggacgggtatgaaaagcca 900

ggtagtcgaaattttcaagaaagctgaagtacctgcccatacttttcggt

gtaaaaggacggaaaattaactggatgaaggctgcaattctggaagctca 950

cattttcctgccttttaattgacctacttccgacgttaagaccttcgagt

ccgtgtcttaacagttagtccatactacgctcaagaactcatctctggag 1000

ggcacagaattgtcaatcaggtatgatgcgagttcttgagtagagacctc

v ttgatagaggcgtggaattgcataaatatcttcgaatgaaaacagtttcc 1050

aactatctccgcaccttaacgtatttatagaagcttacttttgtcaaagg

ggaattattaatggaatggatgttcaagaatggaacccgtctactgacaa 1100

ccttaataattaccttacctacaagttcttaccttgggcagatgactgtt

gtacatcgatatcaaatacgatattaccactgttacagatgctaaaccat 1150

catgtagctatagtttatgctataatggtgacaatgtctacgatttggta

tgatcaaagaagcacttcaggctgctgttggacttcccgtggacagggat 1200

actagtttcttcgtgaagtccgacgacaacctgaagggcacctgtcccta

gtcccggttatcggtttcatagggagattggaggagcagaagggttctga 1250

cagggccaatagccaaagtatccctctaacctcctcgtcttcccaagact

tattctagtggaagctatttccaagttcatggggctcaatgttcagatgg 1300

ataagatcaccttcgataaaggttcaagtaccccgagttacaagtctacc

ttatccttgggactggaaagaagaagatggaggctcagattcttgaacta 1350

aataggaaccctgacctttcttcttctacctccgagtctaagaacttgat

gaagagaagttcccagggaaggcggttggagtggcgaaattcaacgtgcc 1400

cttctcttcaagggtcccttccgccaacctcaccgctttaagttgcacgg

attggctcatatgatcactgctggagctgacttcatcattgtcccaagca 1450

taaccgagtatactagtgacgacctcgactgaagtagtaacagggttcgt

ggtttgagccgtgtggtctcattcagctgcacgcaatgagatatggaacc 1500

ccaaactcggcacaccagagtaagtcgacgtgcgttactctataccttgg

gtccctattgtggcatctactggtggacttgtggacactgtgaaagatgg 1550

cagggataacaccgtagatgaccacctgaacacctgtgacactttctacc

ctacacaggtttccacattggaagattcaacgtcaagtgtgaagttgtgg 1600

gatgtgtccaaaggtgtaaccttctaagttgcagttcacacttcaacacc

atccagatgatgtgatagcaacagcaaaggctgtgacaagagccgttgca 1650

taggtctactacactatcgttgtcgtttccgacactgttctcggcaacgt

gtatatggaacatccgcaatgcaagaaatggtcaagaactgcatggacca 1700

catataccttgtaggcgttacgttctttaccagttcttgacgtacctggt

agacttctcctggaagggacctgcgaggttgtgggagaaggtactattgt 1750

tctgaagaggaccttccctggacgctccaacaccctcttccatgataaca

cccttaatgtggcgggaagtgaagccggaaccgagggtgaagagatagct 1800

gggaattacaccgcccttcacttcggccttggctcccacttctctatcga

cctctggccaaggagaacgtagcgacgccgtga 1833

ggagaccggttcctcttgcatcgctgcggcact

No forbidden restriction sites!

BIOBRICKTM PRIMERS
Forward: CGT gaattc gcggccgc t tctagATG GCA ACT GTG ACT GCT (54ºC)

Reverse: CGT t actagt a TTA TTA CGG CGT CGC TAC G (52ºC)