Team:Waterloo/Notebook/Protocols/Glycerol Stock Preparation

 Glycerol Stock Preparation 

Before you start  - It is important to use aseptic technique when carrying out the procedures

Materials  5 mL culture tube 60% Glycerol Sterile screw-top cryotube

Instructions  1. Inoculate a 5 mL culture tube. 2. Incubate overnight on the shaker. 3. Pipette 500 μL of 60% glycerol culture into a sterile screw-top cryotube. 4. Piptette 500 μL of culture into the cryotube. 5. Label and date cryotube. 6. Vortex. 7. Cover the label with clear tape. 8. Store in -80°C freezer. 9. Add strain to database.