Team:NTU-Singapore/Notebook/4 July 2008



  

=Friday 4 July= {|border="1" style="background-color:#ffffcc;" cellpadding="20"

Hung, Darius

 * Ligation of E7 into empty plasmid vector extracted from GFP plasmid.
 * Gel electrophoresis for the E7 plasmids synthesized by PCR (with 2 blunt ends recognized by EcoRI and PstI).
 * Gel extraction of E7 bands (around 2kb) to remove all the oligonucleotides.
 * Purification for E7 after extracted.
 * Digestion of GFP by EcoRI and PstI. Incubation for 3 hours.
 * Digestion of purified E7 by EcoRI and PstI. Incubation for 3 hours.
 * Gel electrophoresis and gel extraction for GFP to get the empty plasmid vector (around 2kb).
 * PCR purification for digested E7 to remove all the enzymes, buffers and small nucleotides.
 * Ligation of empty plasmid (from GFP) and E7 insert.
 * Transformation and cloning into homemade top10 cells.

Lu Chao, Hung
transformation and cell cloning of LacI-GFP and LacI-RFP again.

Lu Chao
inoculation of LacI-RBS cells.

Choon Kit
documentation of lab protocols.