Team:The University of Alberta/2 July 2008

Today
Chris Tom Jason Saima
 * Made two SDS-PAGE gels for running the purified proteins. Stained with Coomassie and left to destain overnight.
 * Streaked out colonies used in colony PCR for Tom (see below)
 * With help of Saima and Jason, did colony PCR of 22, 23, 25, 35 in I0500 (from diliuted and concentrated Kan plates) as well as 21 and 22 in J61003. Results were ambiguous (bands of wrong sizes, bands in the water controls, etc). They will be sequenced to figure out what's what.
 * Did some dishwashing
 * Learned how to harvest Arabidopsis seeds (its pretty easy!)
 * Made lots and lots of LBA and LBK plates
 * colony PCR of 22, 23, 25, 35 in I0500

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