Constructs tested

Experiment to test the function of old and new Lac promoters

Each of the promoters is used to drive expression of GFP so that fluorescence can be used to measure promoter function. The data demonstrate that the new Lac promoters have improved function over the existing wild type promoter in the Registry. Experiments to test basal function of XOR gate constructs K091173 was coplated with the K09100 Lux receiver. Green fluorescence indicates that at least one of the two hybrid promoters in the XOR construct is driving expression of the LuxI gene.

XOR gate construct K091182 coplated with the K09100 Lux receiver. Green fluorescence indicates that at least one of the two hybrid promoters in the XOR construct is driving expression of the LuxI gene.