Team:Warsaw/Calendar-Main/28 August 2008

Cloning of protein A DNA to pET15b+OmpA-alpha plasmid Michał K.  Colony PCR on colonies from plates with transformation pET15b+A_alpha. Primers used:

AP+NotI and AL+SacI.  Gel electrophoresis (Fig. 1.).</li>  Confirmed transformant colonies inoculated to liquid LB with ampicillin. </li></ol>

<img src="http://2008.igem.org/wiki/images/8/8a/Kolos.jpg" width=300/></a> Fig. 1. PCR on colonies from plates with transformation 1. Marker 2-23. PCR products