Team:Hawaii/Notebook/2008-07-16

= Things we did today =

Transformation

 * Margaret


 * pSB3K3 was restreaked yesterday, and today I am growing them up in LB media in preparation for a plasmid prep.


 * checked transformants from yesterday. The controls came out as expected (positive: lawn, negative:lawn(oops, i didn't add selection to the plate)), neither pSB3K3, pSB1A2, nor pSB1AK3 transformed.


 * I extracted pSB1A2 & pSB1AK3 today with 7uL TE. All 7uL was used to transform DB3.1 cells again.

OriT Ligation

 * Last night, I digested the oriT ligation with PstI, left it overnight. This morning, I stopped the digestion, precipitated the DNA and stored it in TE in the -20C freezer. The protocol can be found here.

Name of Task

 * Margaret, Krystle, Grace


 * Worked on project summary

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"