Waterloo/28 July 2008

08/07/28 Mon

[x] gel of PCR

[x] run check gel of gel extraction product - not successful. looks like no dna - will repeat PCR

[] if above successful, Clone T7 Gene6 with pjet kit

[x] repeat PCR with gene 6 again, in MQ, 5% DMSO, 10% DMSO - run longer program (40 cycles) [x] test Plac rfp, Plac gfp in induced and not-induced conditions (re streak in IPTG and non IPTG plates)

[x] inoculate stabs from iGEM

[x] washing buffer made (for new fermentas kit)