Team:Hawaii/Notebook/2008-09-21

= Things we did today =

Transformation from last week

 * Margaret


 * Included in my last transformation were pSB1A3, de-phosphorylated pSB1A3, and restriction digested (E,P) pSB1A3. All had colonies on DH5 alpha, and I want to see what they are.
 * Colony PCR, 62&deg;C annealing, 1 minute extension.
 * Run 2%gel to compare them to colonies of curious size. I will also run the 8 rep colonies from this transformation that I ran a PCR on yesterday.
 * PCR order: 2 pSB1A3, restriction digested (E,P) pSB1A3, & 2 de-phosphorylated pSB1A3, (+) pSB1A3 from a plasmid prep, and (-) water. All using VF2&VR primers.

Transformation of ligations into DH5&alpha;

 * Grace


 * Used 5&mu;l of ligation reaction from yesterday

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"