Team:Montreal/Notebook/May

May 22
Prepared TOP10 competent cells for eventual transformation. Performed Mini-prep on Reporter+ Cells Performed Digest and Gel on Reporter plasmid extract -- no DNA present, suggest follow-up maxi-prep

May 23
Transformed Top10 cells with Puc19 to ensure that the competent cell procedure was successful. Growth was observed, therefore procedure was successful.

May 25
Diluted Reporter cells 1/1000 with 5ul Kan/mL culture for 16h incubation at 5:30pm. To be used for Maxiprep at 9:30am-1:30pm.

May 28
Ran gel on Elowitz Reporter DNA cut with EcoR1; 2 bands 0.7 kb and 2.0 kb, confirms identity of reporter DNA. Seeded syn-I and J-40001 into amp/kan LB and kan LB.

May 29
Growth of J-brick in culture - No growth of I-brick on culture Seeded J-brick for Midi-Prep in 40mL LB with ampicillin Transformed TOP10 cells with both I brick and Reporter Plasmid