Protocols Electrophoresis

Making Gel 1% gel by dilution => 0.6g Low melt PCR agarose => 1.5mL 40x TAE => 58.5mL ddH2O ==> 1.5ul Ethidium Bromide Running Gel 20ul digestion product Add 5ul of 5x loading buffer Load lane 10ul ladder marker Run gel for 30-45 minutes Protocols