Team:Chiba/jk/γ/week 2

>実験ログ

>出力班

24,August 2008
Transformation
 * BBa_J63001(yeast optimized YFP)-->colony

Digestion
 * BBa_F2620(1)①
 * BBa_F2620(2)②
 * Menbren Venus YFP③

-->37℃ 30min

-->135V 30min

25,August,2008
PCR
 * BBa_E2030①
 * BBa_J33202②
 * BL21③
 * BBa_J52008④
 * BBa_I712019⑤

-->Gel Check

Mini prep
 * Venus
 * BBa_F2620

Transformation PCR
 * Venus
 * PUC19
 * pGFPUV
 * pGEX Venus YFP
 * Membren Venus YFP
 * BBa_J63001①

26,August,2008
Gel Check
 * BBa_F2620
 * BBa_F2620
 * BBa_J63001

Digestion
 * vector:BBa_F2620


 * insert:BBa_J52008,BBa_E2030

-->37℃　3hour -->SAP -->37℃ 30min -->65℃ 15min -->Zymo Clean

Ligation


 * vector:BBa_F2620 insert:BBa_J52008①
 * vector:BBa_F2620 insert:BBa_E2030②
 * Negative control:BBa_F2620③

-->RT 2hour

-->Transformation(XL10G)

-->37℃ over night

-->colony PCR

-->Mini prep

28,August,2008
Time Responce(liquid)

29,August,2008
TIME RESPONCE(solid)

30,August,2008
Transformation


 * mcherry

PCR


 * BBa_J52008(rLuc)

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