Team:Valencia/Notebook/September



September 2nd
-We carried out the following experiment: Four strains in LCCs with SP medium. 1 % Galactose induction at the beginning. Initial O.D. 0,7 275 rpm.

-Team meeting: Checking project progress.

September 3rd
-We characterized LCC temperature evolution with water.

September 5th
-We characterized LCC temperature evolution with SP medium and SP medium with bleach (to avoid contamination).

September 8th
- We observed the culture after the experiment under the microcope. There was no contamination but we did observe some elongated microorganisms, same size as S. cerevisiae.

- We discovered that our plaque with the four strains for the following experiment had suffered cross contamination.

September 9th
-We carried out the following experiment: Four strains in LCCs with SP medium. 1 % Galactose induction at the beginning. Initial O.D. 0,6 270 rpm. Initial temperature 31 ºC. Too high.

September 11th
-We repeated the same experiment: Four strains in LCCs with SP medium. 1 % Galactose induction at the beginning. Initial O.D. 0,6 270 rpm. Initial temperature 31 ºC. Too high again.

September 15th
-We repeated the same experiment: Four strains in LCCs with SP medium. 1 % Galactose induction at the beginning. Initial O.D. 0,6 270 rpm. Initial temperature 28 ºC. Perfect!

September 20th
-Trying to reproduce the previous experiment...     Four strains in LCCs with  SP medium. 1 % Galactose induction at the beginning. Initial O.D. 0,6 270 rpm. Initial temperature 28 ºC. But room temperature too high

September 24th
- We repeated the same experiment: Four strains in LCCs with SP medium. 1 % Galactose induction at the beginning. Initial O.D. 0,6 270 rpm. Initial temperature 28 ºC.

September 26th
- Team meeting: Checking project progress and inmediate future actions.

September 27th
- We tryied and increased initial O.D.      Four strains in LCCs with  SP medium. 1 % Galactose induction at the beginning. Initial O.D. 2 270 rpm. Initial temperature 28 ºC.

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