Team:Warsaw/Calendar-Main/9 May 2008

Preparation of pMPMT5+AID construct Michał K.

  Restriction digest of pTrc99A-AID with HindIII and NcoI (1x Tango buffer).

 DNA electrophoresis and isolation of DNA encoding AID from proper band (600 bp). Fig. 1.

 Restriction digest</a> of pMPM-T5</a> with HindIII and NcoI (1x Tango buffer), dephosphorylation</a> (CIAP).</li>  DNA electrophoresis and isolation</a> of pMPM-T5</a> vector from proper band (4250 bp). Fig. 2</a>.</li>

Electrophoresis to estimate the concentration of isolated DNA.</li>  Ligation</a> of isolated DNA fragments. </li> </ol> <img src="http://2008.igem.org/wiki/images/8/89/Gel_out_aid_WAW.jpg" width=170/></a> Fig. 1. 1-DNA ladder; 2-pTRC99a-AID digested with NcoI and HindIII <img src="http://2008.igem.org/wiki/images/0/0f/Gel_out_pmpm_WAW.jpg" width=200/></a> Fig. 2. 1-DNA ladder; 2-pMPM-T5 digested with NcoI and HindIII