Team:The University of Alberta/2 October 2008


 * Tom
 * ETFA/B already have RBS site, they are ready for use
 * setup overnight of 21-25(1-4); ETFA/B (5-6)
 * redo 21-25 ligation tomorrow
 * religate 21+22+23(S/P) AND 24+25(X/P)
 * sequence ETFA/B with Vf and Vr

David
i picked candidate colonies from yesterdays transformations to grow up overnight in LB amp.

pTET+BisdA pTET+BisdB