File:Jr rmf 3.jpg

Cell viability at increasing IPTG concentration. BL21 DE3 cells holding the pET28a-RMF plasmid with mutations R197A; R197F; T276A; T276F; R197A T276A; R197A T276F; R197F T276A; R197F T276F were plated on LB-agar with kanamycin without IPTG, with 1mM IPTG and with 10mM IPTG