Newcastle University Wetlab/29 August 2008

Friday 29th August

 * Today we restricted our pUC57-ncl08 plasmid. In one reaction we used EcoR1 and Nhe1, and in other we used BglII and Nhe1.  We also carried out a restriction with just BglII to check for the correct sized linear DNA.  In addition we ran unrestricted plasmid on the gel.

Expected band sizes: * For digestion with BglII only: ~ 4.9kb * For digestion with Nhe1 and EcoR1: ~ 2.2kb and 2.7kb * For digestion with Nhe1 and BglII: ~ 2.2kb and 2.7kb


 * As you can see, the restrictions appear successful.