Alberta NINT/29 July 2008

< Back to notebook

< Previous entry | Next entry >

lab work (SD): Isolated DNA (K102031 and K102032) from 28/07/08 LB + amp50 culture tubes using QIAprep spin miniprep protocol, using 5 ml of culture and a minelute spin column, eluting in 15 ul of Buffer EB            to maximize the concentration of DNA obtained. Digested K102032 and K102031 with EcoRI + PstI. Ran on 2% agarose gel (110V 50 minutes) and excised the 50 bp fragment from K102032 and the 3 kb             fragment from K102031. Extracted DNA from gel usig QIAquick gel extraction protocol. Ligated K102032/E+P with pSB4A5 (from K102031 /E+P)(Negative control K102031 /E+P).

lab work (JD): Began creating new input plasmids Anneal oligos: TAIn_2, TAIn_3, TAIn_6, TAIn_7 Digest oligos with XhoI and HindIII Digest K102011 with XhoI and HindIII Nucleotide removal kit for TAIn_X/XhoI and HindIII PCR purification kit for K102011/XhoI and HindIII