Team:Hawaii/Notebook/2008-07- 7

= Things we did today =

Biobrick Extraction

 * Margaret


 * Extracted Biobricks BBa_E0240, BBa_I4032, BBa_pSB3K3
 * Transformed into E. coli
 * 7/08/08: checked and there are no transformants (I made a mistake about the death protein...), decided to drop this experiment until DB3.1 cells come in.

Oligonucleotide experiment

 * Grace


 * Ligated annealed products using 10-2 dilution of products
 * Reran on EtBr stained 4% agarose gel (result: light bands for annealed products, correct size; no bands for ligated product)
 * Ran annealed products (10-1 dilution) on SYBR Safe stained 2% agarose gel
 * Restriction digested ligation from 7/3/08 using XbaI and PstI in NEBuffer 3
 * Reran on EtBR stained 4% agarose gel

Plasmid prep

 * Krystle


 * Grew up E. coli from cryostock and performed an alkaline lysis plasmid prep for C0012

PCR of pRL1383a parts



 * Margaret


 * Amplification of rep and mob regions. Neither of these parts came out very well, so trying them with red and green taq.

= Discussion =

= Quote of the Day =  ''"I assume if there are jellyfish, it's not completely pelleted?" - KS ''