Team:Hawaii/Notebook/2008-08-22

= Things we did today =

Verification of transformants

 * Grace


 * Colony PCR of transformants
 * Ran PCR reaction on 2% agarose gel
 * Can't tell 15bp difference between GFP and rbs+GFP
 * Restreaked single colony and inoculated for plasmid prep to confirm
 * nir+rbs colonies #11-19 appear promising (larger than nir)
 * Restreaked colony #19 and inoculated for plasmid prep to confirm

Plasmid prep of J33207

 * Grace

Overnight RE digest

 * Grace


 * BB-pRL1383a and J33207 with XbaI and PstI
 * pSB1A2 with EcoRI and PstI
 * GFP, GFPf, nir with EcoRI and SpeI

Made LB+amp100 plates

 * Grace

Ligation
Margaret
 * ligation of rep+pSB1A3, P1lytic region+pSB1A3, aada(BB&pRL1383a)+pSB1A3

Omega interposon

 * Margaret
 * streak on Sm&Sp plate to confirm insertion.

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"