Team:Mississippi State/9 July 2008


 * 1) Make 500uM stock solution of Upstream and Downstream primers.
 * 2) Make working solution of primers: 1ul stock + 49ul ddH2O
 * 3) Prepare PCR mix (for each sample, BKM and RP): 39ul ddH2O + 5ul Buffer + 1ul dNTP + 1ul Upstream + 1ul Downstream + Template + Taq = 50ul total
 * 4) Run PCR
 * 5) Prepare 2nd PCR mix using 1ul of 1st PCR product
 * 6) Prepare and run 1% Agarose Gel w/ both 1st and 2nd PCR products