Utah State/20 June 2008

Today we looked at the electrophoresis results from yesterday's pictures. The RFP, Banana, Wintergreen, and CFP DNA runs were as expected and are indicative of successful transformations. The Salicylate Generator, YFP/CFP, and GFP DNA runs were not as expected. The Salicylate Generator well showed no DNA whatsoever, which doesn't necessarily mean the transformation didn't work - possibly just an error in the DNA isolation. It appears the GFP transformation did not work at all, as it had only one band in the gel. Another GFP transformation will be done next week with a different GPF insert.