Wisconsin: Lignin Project/23 June 2008



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 * Team Sorbitol:

Decided to use Herculase instead of TAQ in the PCR reactions (Herculase has proofreading ablitity) Ran a new PCR reaction using the specific volumes designated by the Herculase designers. No Bands formed.

Team Fungus: Ran a touchdown PCR of Carbon and Nitrogen-limited cDNA using Herculase II polymerase Ran PCR products through 0.9% agrose gel at 100V: bands present for both at about 1.1kb Grew overnight culture of pET28a