Team:NTU-Singapore/Modelling/Parameter



=Parameters=

Lactose controlled production of E7 + Imm

 * 1) LacI = A
 * 2) Lactose =B
 * 3) E7 = C

Iron and Ai2 controlled production of Lysis

 * 1) Ai-2			:	A
 * 2) ai-2-phos		: 	B
 * 3) LsrR			:	C
 * 4) SupD derivatives	: 	D
 * 5) T7ptag			:	E
 * 6) Lysis			:	F

=Parameter Estimation= Estimation of different parameters

Assumptions
 * 1) Rate of transcription is dependent on length of gene
 * 2) Number of amino acids is 1/3 of the number of nucleotides in a gene
 * 3) Rate of Translation is dependent on number of nucleotides
 * 4) For each gene mRNA = 10 at steady state
 * 5) Rate of degradation of average mRNA = 1100/ 5 min
 * 6) Rate of degradation of protein is equivalent to time for cell division i.e. 40 min



Detection & Lysis system
Some of the parameter values were obtained via trial and error as the values could not be found in literature. The values are chosen to produce the desired behaviour of the graph. More work has to be done to actually find out how much lysis protein quantity is really required for lysis of the cell as this is still an unknown. Readers may find some of the parameters unrealistic but the purpose of this exercise is to observe how changing certain parameters may affect the system overall output. We advise the reader to look more into the behaviour of the system rather than be focused upon the numerical values.