Team:NTU-Singapore/Notebook/13 June 2008



  

=Friday 13 June= {|border="1" style="background-color:#ffffcc;" cellpadding="20"

Chin Chong

 * BL21 cells, containing the LacI-GFP, that were inoculated on thursday were not growing well as they were placed too long in the incubator
 * Regrew/Inoculate the BL21 cells at 5pm so that overnight growing of 16hrs would be acheived
 * Prepared 20 plates of agar containing Chloranphenicol and 20 plates of agar containing Kanamycin
 * Approached Phd students from BIE whom had experience in using the multiplate Fluroescene and luminescene reader
 * Varified the correct usage from the student, Zhang Zhengwen, who adviced on some slight modifications to the protocol set for the characterization experiment
 * Received the primer designs from 1st Base for: supD, T7ptag and direction checks for T7ptag as well as that for LsrA