Team:The University of Alberta/6 August 2008

Chris
 * Got some strange results from the PCR I did yesterday. There was a band in the digested sample BUT it wasnt the size of GFP; rather it was ~1500bp, the expected size for Tryp. Even odder is that the same band is in the uncut sample as well, in addition to the expected GFP sized band. I discussed this with Doug for quite some time and we were unable to come to a conclusion about what's going on with Tryp. I figure tomorrow I'll just go back to the drawing board and start over...

Saima  Jason cam35sr +PR and CAMR and MCS
 * Mini-preped the O/N's of Col. 4,5,& 6 of I0500+ER01 and I0500+ER02. Can be found in Mini-prep Box 3.
 * Sequenced Minipreps of Col.4 & Col.5 of I0500+ER01, and Col.4 of I0500+ER02. To be taken to MBSU on Thrursday. Results to be back by Friday.
 * Set up the O/N's for I0500+ER01 col.6, I0500+ER02 col.4, I0500 in new vector, GFP+B0034 Col.1a & 1b.
 * overnight of transformants (MCS+CanR, LacIQERE+TETR)
 * transforming ligations