Edinburgh/Notebook/Ligation products

< Back to Notebook

Ligation Products
L1: Edinbrick1+dxs (26.08.08)

L2: Edinbrick1+appY (26.08.08)

L3: Babel1+glgC (26.08.08)

L4: Babel2+glgC (26.08.08)

L5: Edinbrick1+appY repeat (initially labelled L4 in error) (04.07.08)

L6: Self ligation of mutagenic PCR P6 (BABEL2+glgC-mut1). (07.07.08)

L7: Self ligation of mutagenic PCR P7 (BABEL2+glgC-mut2). (07.07.08)

L8: Ligation of synthetic ribosome binding site to M2 (pSB1A2+dxs, BBa_K118000). (09.07.08)

L9: Ligation of Edinbrick1 with crtB. (10.07.08)

L10: Ligation of Edinbrick1 with crtI (mutant lacking PstI sites, I hope). (10.07.08)

L11: repeat ligation of synthetic ribosome binding site to M2 (pSB1A2+dxs, BBa_K118000). (11.07.08: Yan, HX)

L12: rbs ligated with pSB1A2+appY (M15). (14.07.08)

L13 and L14: self ligations of P13 and P14 (BABEL2+glgC-mut1,2). (14.07.08)

L15: ligation of Edinbrick1 with rbs+dxs fusion PCR product (P12). (14.07.08) - Failed

L16: Ligation of Edinbrick1 with rbs+appY fusion PCR product (P15) (17.07.08) - Failed

L17: Ligation of rbs to pSB1A2+crtB (M36) (22.07.08: OG, Yan)

L18: Ligation of rbs to pSB1A2+crtI (M42) (22.07.08: OG, Yan)

L19: pSB1A2+rbs+dxs ligation product (From P12) (25.07.08: Yan, OG)

L20: pSB1A2+rbs+appY ligation product (From P15) (25.07.08: Yan, OG)

L21: pSB1A2+rbs+crtB ligation product (From P28) (25.07.08: Yan, OG)

L22: pSB1A2+rbs+crtI ligation product (From P29) (25.07.08: Yan, OG)

L23: pSB1A2+pZntA ligation product (From P31) (25.07.08: Yan, OG)

L24: Ligation of crtE to pSB1A2 (25.07.08: CF)

L25: Ligation of glgC-mut1,2 (P36) to Edinbrick1, (28.07.2008: AM)

L26: Ligation of P15 (rbs+appY) to Edinbrick1. (30.07.2008: HX)

L27: Ligation of P28 (rbs+crtB) to Edinbrick1. (30.07.2008: HX)

L28: Putative cenA ligated to Edinbrick1. (30.07.08: AM)

L29: Putative cex ligated to Edinbrick1. (30.07.08: AM)

L30: Self-ligation of P50 (3rd mutagenesis of glgC-mut1,2) (05.08.08: HX)

L31: Ligation of P36 (glgC-mut1,2, hopefully, could be P39) to Edinbrick1 (05.08.08: OG)

L32: Ligation of rbs+crtB (M67) to rbs+crtI+pSB1A2 (M50) to create pSB1A2+crtB+crtI (07.08.08: AM, Yan)

L33: Ligation of PcstA (P54) to Edinbrick1. (08.08.08: OG)

L34: Ligation of pSB1A2+rbs+dxs with rbs+crtE to make pSB1A2+dxs+crtE. (08.08.08: Yan)

L35: Ligation of pSB1A2+rbs+dxs with rbs+LIMS1 to make pSB1A2+dxs+crtE.(08.08.08: Yan)

L36: pSB1A2+crtY (P59 self-ligation). (09.08.08: CF)

L37: pSB1A2+rbs+crtY (P60 self-ligation). (09.08.08: CF)

L38: crtE+pSB1A2 (M79 into Edinbrick1) (12.08.08: AH)

L39: cex into Edinbrick1 (13.08.08:YAN)

L40: cenA into Edinbrick1 (13.08.08:YAN)

L42: dxs+LIMS (M137) to appY (M82). (18.08.08: YAN)

L43: crtB+crtI (M109) to appY (M82). (18.08.08: YAN)

L44: PcstA (M130) to lacZ. (18.08.08: YAN)

L45: glgC mut 1+2 (M71A) to SOB2 (18.08.08: AM)

L46: glgC mut 1+2+3 (M71) to SOB2 (18.08.08: AM)

L47: glgC mut 1+2+3, a.k.a. glgC16 (M72) to SOB2 (18.08.08: AM)

L48: appY into dxs+LIMS (19.08.08 YAN)

L49: appY into crtBI (19.08.08 YAN)

L50: Self-ligation of P78 (SOB2-glgC mut 1+2) (20.08.08 AM)

L51: Self-ligation of P79 (SOB2-glgC mut 1+2+3) (20.08.08 AM)

L52: Self-ligation of P80 (SOB2-glgC mut 1+2+3) (20.08.08 AM)

L53: Self-ligation of P81 (SOB2-rbs-glgC mut 1+2) (20.08.08 AM)

L54: Self-ligation of P82 (SOB2-rbs-glgC mut 1+2+3) (20.08.08 AM)

L55: Self-ligation of P83 (SOB2-rbs-glgC mut 1+2+3) (20.08.08 AM)

L56: lacZ into PcstA (20.08.08 YAN)

L57: appY into crtBI (28.08.08 YAN) L58: Self-ligation of P91 (03.09.08: YAN) L59: Self-ligation of P92 (03.09.08: YAN) L60: Self-ligation of P93 (03.09.08: YAN) L61: Self-ligation of P94 (03.09.08: YAN) L62: dxs+crtE into crtBI (09.09.08: YAN) L63: P90 (bglX) + Edinbrick 1 (09.09.08: AM) L64: P64 (cenA) + Edinbrick 1 (09.09.08: AM) L65: Plac+ dxs+LIMS+appY (CF) L66: PcstA+lacZ (CF) L68: self-ligation of P91 (sob-SU1)(CF, 22-9-8) L69: self-ligation of P93 (sob-ISO2)(CF, 23-9-8) L70: self-ligation of P96 (mutagenesis of cex)(CF, 23-9-8) L73: ligation of P65 (cenA) to Edinbrick1. (CF, 24-9-8) L74: ligation of P90 (bglX) to Edinbrick1. (CF, 24-9-8) L75: ligation of P99 (rbs+glgC) to Edinbrick1. (CF, 25-9-8) L76: ligation of P100 (rbs+glgC16) to Edinbrick1. (CF, 25-9-8) L77: ligation of crtBI to dxs.crtE. (CF, 26-9-8) L78: ligation of xylE (BBa_J33204) to PcstA. (CF, 29-9-8) L79: ligation of Plac+lacZ'  (BBa_J33207) to rbs+glgC. (CF, 29-9-8) L80: ligation of Plac+lacZ'  (BBa_J33207) to rbs+glgC16. (CF, 29-9-8) L81: ligation of rbs to cex. (CF, 30-9-8) L82: ligation of rbs to cenA. (CF, 30-9-8) L83: ligation of Plac+lacZ'  (BBa_J33207) to crtBI. (CF, 30-9-8) L84: ligation of Plac+lacZ'  (BBa_J33207) to dxs.crtE. (CF 30-9-8) L87: ligation of bglX to Edinbrick2. (CF, 7-10-8) L88: ligation of P105 (bglX with complete suffix) to Edinbrick1. (CF, 10-10-8) L89: ligation of P105 (bglX with complete suffix) to Edinbrick2. (CF, 10-10-8) L90: self-ligation of P107, mutagenesis of SU1. (CF, 10-10-8) L91: self-ligation of P107, mutagenesis of ISO2. (CF, 11-10-8) L92: repeat L83 (CF, 12-10-8) L93: repeat L84 (CF, 12-10-8) L94: ligation of Plac+lacZ'  (BBa_J33207) to rbs+cenA (later turned out to be wrong clone). (CF, 29-9-8) L95: ligation of Plac+lacZ'  (BBa_J33207) to rbs+cex (later turned out to be wrong clone). (CF, 29-9-8) L96: ligation of Plac+crtBI to dxs.crtE. (CF, 16-10-8) L97: ligation of Plac+dxs.crtE to crtBI. (CF, 16-10-8) L98: repeat of L90. (CF, 16-10-8) L99: repeat of L91. (CF, 16-10-8) L100: ligation of rbs to bglX. (CF, 22-10-8) L101: ligation of P110 (rbs+cex) to Edinbrick1. (CF, 23-10-8). L102: ligation of P111 (rbs+cenA) to Edinbrick1. (CF, 23-10-8). L103: ligation of P112 (rbs+bglX) to Edinbrick1. (CF, 23-10-8). L104: ligation of P113 (dxs.crtEBI) to Edinbrick1. (CF, 27-10-8).