EPF-Lausanne/14 July 2008

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Week 2 of our lab experience starts. This Journal is starting with it as well.

Today, we are working on expressing the predator-prey system in E. Coli. We got colonies for the predators in two different antibiotic resistances (Kan and Cam). Control is growing well.

We do the transformation to get the plasmids pHD (3 CamR), pLD (KanR), and the prey E. coli which did not work before (in Cam and Kan). We also use the punched-out DNA no.4c-1000 and 2E-1000 from the iGEM notebook to transform the cells with ampicillin resistance and amplify the fragments.

We prepare Top10 cells for competence this week, so started growing them in SOB.