Minnesota/24 June 2008



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 * 2. Plating from 6-23-08 transformations again.
 * a. Since plating of the 6-23 transformations provided no colonies for parts 15-18, the remaining cells from those transformations were re-plated. 75 uL of cell culture was spread on each of two plates for each culture; plates contained LB media and the corresponding antibiotic. A metal spreading tool was used to spread the culture suspension on the plates, and this was sterilized between each sample by dipping it in 100% ethanol (EtOH) and flaming it. 75 uL cell culture was pipetted on, and spread around plate.
 * b. Plates were placed at 37C in an incubator and allowed to grow overnight.
 * 3. Sequencing primers ordered on 6-20-08 were picked up. All primers were diluted to mircomoles according to the following additions of sterile H20:
 * b. Plates were placed at 37C in an incubator and allowed to grow overnight.
 * 3. Sequencing primers ordered on 6-20-08 were picked up. All primers were diluted to mircomoles according to the following additions of sterile H20:
 * 3. Sequencing primers ordered on 6-20-08 were picked up. All primers were diluted to mircomoles according to the following additions of sterile H20:
 * 3. Sequencing primers ordered on 6-20-08 were picked up. All primers were diluted to mircomoles according to the following additions of sterile H20: