Team:ESBS-Strasbourg/24 July 2008

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WetLab
Manuel Sandra => find them in the -20°C fridge in our lab in tubes with different concentrations
 * tetR, lacI PCR on BB (Primers, VFPrimers)
 * Pfu Pol PCR on pGEX lacI containing plasmid
 * ON of BB:
 * J63002
 * J63003
 * J63005
 * J63008
 * Maxiprep of plasmid p10101A2 with final amount of 70 µg and a good quality (ratio 260/280 = 1,7)

Paul Digestion of tetR/lacI/c1 plasmids, in order to check if the biobricks are really there. Used of EcoR1 and Spe1.

=> no results, probably not enough DNA for the digestion. To redo next day.

General

 * Animation (MM)