Waterloo/3 September 2008

080903 Wed

[x] run NotI diagnostic gel: - pJET(gene6) vs. pSB1A2(RBS-gene6?) --> [if looks okay, send for sequencing] - cI'? (both C0051'? and C0052'?) (in good box) vs. cI --> --> [if looks okay, send for sequencing] - pConst-rfp"? (purgatory) vs. rfp"? --> [if okay, send both for sequencing] - "stab" gfp (preppred pDNA) - pTetR (purgatory) - pKNG101 digest with E,X,S,P,N,E+X to see which biobrick enzymes we can use for ligation into pKNG101