Team:Hawaii/Notebook/2008-10- 2

= Things we did today =

Verification of transformants

 * Grace


 * Colony PCR of transformants\
 * Most lanes blank; lanes with bands are mostly faint. Didn't load enough PCR product? Will reload gel tomorrow with remainder of PCR product.
 * Restreaked:
 * rbs+GFP+tt #9, 10, 16, 17
 * slr1+GFPf+tt #13
 * nir+rbs+slr1+GFPf #1, 2, 15, 17

Plasmid Prep

 * Margaret
 * oriV 3, 4, & 6

Restriction Digest

 * Margaret
 * oriV3 E,S
 * oriV4 E,S
 * oriV6 E,S
 * oriV11 E,S
 * plac/rbs/rep 8 X,P
 * pSB1A3 E,P
 * oriT E,S, and another sample of oriT with X,P

Culture

 * Margaret
 * ap1, and ap2. Because I need to digest them with E,S so that I can use as a front insert

PCR Verification

 * Margaret


 * 10 more colonies of plac/rbs/rep
 * check if SAP and SAP buffer is contaminated

Sequencing

 * Margaret


 * oriV 3, 4, 6
 * plac/rbs/rep 8 with 11 primers to cover whole sequence

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"