Team:Chiba/Sender experiments/Senders(JW1908) T9002(JW1908)



Sender culture：100&mu;L,Receiver culture：1000&mu;L

 * Fig. 1,Fig. 2
 * Results
 * Green fluorescence intensity didn't increase in the culture containing BBa_K0840010(plac+CinI+LVA).We thought BBa_K084010 didn't work properly or LuxR gene didn't interact with signal molecules synthesized by BBa_K084010.
 * Others responded at the same time(4 hours after induction).Final fluorescence intensity differd.

Sender culture：500&mu;Lm,Receiver culture：500&mu;L

 * Fig. 3
 * Results
 * Response time and final fluorescence intensity showed no significant difference.
 * Discussion
 * We thought AHL concentration was quickly reached the threshold concentration.The fluorescence intensity increased as gfp maturured.


 * Fig. 4
 * Results
 * No significant difference in fluorescence intensity between the culture containing BBa_K084007(plac+RhlI) gene transformed cells and the culture containing BBa_K084008(plac+RhlI(LVA)) gene transformed cells.
 * Discussion
 * We thought that the rate of AHL synthesis by each autoinducer synthase was much faster than the rate of its degradation by protease.

Sender culture：500&mu;L,Receiver culture：500&mu;L



 * Fig. 6
 * Results
 * No significant difference in fluorescence intensity between the cultures containing BBa_K084007(plac+RhlI) gene transformed cells and the culture containing BBa_K084008(plac+RhlI(LVA)) gene transformed cells.
 * Discussion
 * We thought that the rate of AHL synthesis by each autoinducer synthase was much faster than the rate of its degradation by protease.

Sender culture：100&mu;L,Receiver culture：1000&mu;L

 * Fig.7
 * Results
 * No significant difference in fluorescence intensity between the culture containing BBa_K084007(plac+RhlI) gene transformed cells and the culture containing BBa_K084008(plac+RhlI(LVA)) gene transformed cells.
 * Discussion
 * We thought that the rate of AHL synthesis by each autoinducer synthase was much faster than the rate of its degradation by protease.

Sender culture：10&mu;L,Receiver culture：1000&mu;L

 * Fig.9
 * Results
 * The final fluorescence intensity of the culture containing BBa_K084008 transformed cells which express LVA-tagged RhlI protein was lower than the culture containing cells which express untagged RhlI protein.It shows that LVA-tagged RhlI protein was degraded by LVA-specific protase.However,fluorescence intensity of both cultures increased at the same time.
 * Discussion
 * We thought it was because the AHL concentration was quickly reached the threshold concentration and cells began to express gfp.After 2 hours,fluorescence intensity started to increase.

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