Team:Rice University/Notebook/9 June 2008

=Monday 9 June=
 * Selim Sheikh:
 * Designed 2 sets of sequencing primers (using Vector NTI Advance 10 http://www.invitrogen.com/site/us/en/home/LINNEA-Online-Guides/LINNEA-Communities/Vector-NTI-Community/Sequence-analysis-and-data-management-software-for-PCs.html) to be used in PCR of lambda DNA to amplify target regions:
 * Primer Set 1:
 * product of length 538
 * contains region of the molecule from 20481 to 21018
 * Tm = 81.7 C   TaOpt: 60.4 C    GC: 56.3
 * area of interest = 20833
 * sense primer: GAGACGAATGCCAGGTCATCTGAAA <---primer name: STF L
 * length: 25    Tm = 60.3 C     GC = 48.0
 * antisense primer: AAATCTGGATCATTCCCGAGCGCTG <---primer name: STF R
 * length: 25    Tm = 64.9 C     GC = 52.0
 * Primer Set 2:
 * product of length 309
 * contains region of the molecule from 23341 to 23649
 * Tm = 70.7 C  TaOpt: 51.4 C    GC: 31.7
 * area of interest = 23539
 * sense primer: ATCACATCGTCACCCATTGGATTGT <---primer name: ATTP L
 * length: 25   Tm = 60.1 C    GC = 44.0
 * antisense primer: CGATTTAGAAATGTATAGCGAGGCA <---primer name: ATTP R
 * length: 25   Tm = 55.9 C    GC = 40.0


 * Taylor Stevenson
 * XL1-Blue MR cells were prepared for phage infection as specified in packaging manual and infected with phage at roughly a 1/10 pfu/cfu ratio.  Resulting phage/bacteria mixture was incubated @ 37*C for 20 min and then streaked onto an LB plate.  Plate was incubated @ 30*C O/N.
 * Result-approximately 100 possibly lysogenic colonies grew on plate.

