Team:Rice University/Notebook/17 July 2008

=Thursday, July 17th= (1kb standard, BleoC PCR product, Stf PCR product) 
 * David Ouyang
 * Digest miniprep amber suppressor
 * ligate in front R0040
 * transform!
 * Taylor Stevenson
 * Primers for lambda phage genomic modification have finally arrived (NOTE: to view primers download this link and change extension to .doc (word file))
 * PCR conditions
 * 1uL of template (miniprepped BleoC in pSBA13, and NEB lambda DNA)
 * annealing temp = 55*C
 * extension time = 3min
 * 30 cycles