Template:Team:UC Berkeley/Notebook/MJV construction/corrected assembly vector

Removing attR2 point mutation in pBjh1601CA PCR mv042F/mv042R on pBjh1601CA     (3861bp, gp, BglII/MfeI) Sub in pBca1256-attR2                (EcoRI/BamHI/DpnI, 2472+151, S) Product is pK112128CA --- mv042F	Forward oligo for pBjh1600CA to patch attR2 (BglII) gcagtAGATCTcttacAGATCCcggttatcc mv042R	Reverse oligo for Bjh1600CA to patch attR2 (MfeI) gcagtCAATTGctgcagactggctgtg