Team:Hawaii/Notebook/2008-09-10

= Things we did today =

Construction of p+r + s(+gf)

 * Grace


 * Ran last night's RE digest on gel
 * Could not resolve nir+rbs, plac+rbs, slr1, pilA (too little DNA? flanking bands also close to size of desired band)
 * Cut out slr1+GFPf, pilA+GFPf bands
 * Treated RE digested pSB1A3 with SAP
 * RE digest overnight:
 * EcoRI + SpeI:
 * nir+rbs (PCR product)
 * SpeI + PstI:
 * nir+rbs (plasmid prep)
 * plac+rbs (plasmid prep)
 * XbaI + PstI:
 * slr1 (PCR product)
 * pilA (PCR product)

Preparation for sequencing

 * Grace


 * plac + rbs colonies 1, 2, 10, 12 (#10 is potential successful transformant)
 * C0012 derived vector used in plac+rbs 3A ligation, dephosphorylated colony 2 and phosphorylated colony 1
 * slr1 + GFPf
 * pilA + GFPf, 8/12 and 8/13 transformations

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"