Waterloo/5 September 2008

080905 Fri

[x] analyze Thursday's gel; select which parts to move forward with [x] redo diag digest and gel of gene6 vs. putative gene6' - gene6' looks too much larger than gene6 than it should [x] full digest (3.5 h): a) vector to clone pRecA b) parts chosen to move forward with c) synthesized parts [x] gel extraction of a) and b) (2 - 2.5 h) [x] ligation of a) with b) (0.5 - 1 h)

[] make media (2 h)

[] transformation of ligations and pDNA of synthesized parts [] reorganize freezer boxes

Crossover PCR: - design/order primers for:
 * gene6"
 * cI"