Team:Mississippi State/LiPA Amplification

=LiPA Amplification=


 * primers: LiPA1(lpoA_EcoRI), LiPA2(lpoA_NotI)
 * Use PCR tube, and add reagents in following order:
 * 1) 40ul ddH2O
 * 2) 5ul Pfx50 Buffer
 * 3) 1ul dNTP
 * 4) 1ul each of LiPA1 and LiPA2.
 * 5) 1ul cDNA
 * 6) 1ul Pfx50 DNA Polymerase
 * Label tube "LiPA"
 * hit it with a vortex
 * short spin (5sec)
 * Put in PCR Machine.
 * Wait until done, remove and make PCR Gel