Team:Mississippi State/Making LB Media for Plates


 * Making LB Media for plates and making LB broth are exactly the same, except for the addition of Agar to plate media.


 * 1) For every 100ml LB to be made, get 2.5g LB powder.
 * 2) !!!Important!!! Only do this step if making media for plates. For every 100ml LB to be made, get 1.5g Agar.
 * 3) Combine LB powder and 100ml water in 250ml autoclaved flask.
 * 4) !!!Important!!! Only do this step if making media for plates. Add Agar.
 * 5) Cover with foil and autoclave tape.
 * 6) Swirl until dissolved.
 * 7) Autoclave: Fill water in autoclave to top of from lip. Close, set time to 20-25min.  It will usually be 40-50min before you can open it.  Make sure exhaust is set to Slow(Liquids).
 * 8) After 40-50min., put on heat gloves and slowly open machine. Watch out for steam!!!
 * 9) Remove flask, place on metallic surface until it cools.
 * 10) If making LB Broth, you are done at this point. Close bottle.
 * 11) Don't allow Media to cool too much, when warm to touch, pour into plates.
 * 12) Get 1 plate for every 20-25ml Media.
 * 13) Pour media into each plate until surface is covered.
 * 14) If media remains, distribute evenly among other plates.
 * 15) Allow plates to cool, then flip upside-down.
 * 16) Wait overnight.