Team:University of Ottawa/11 July 2008

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Today in the Lab
Matt
 * PTP2 Digestion
 * <li> Ran two digestion's of the PTP2 in parallel with one another: AatII/NcoI, EcoRV/NcoI. Neither Gel gave proper band sizes.
 * PCR confirmation
 * <li> PCR confirmation of 97 plasmid showed up nicely on the gel, confirming that the integration was a success.

Chris
 * Confirmation of AtCRE
 * <li> ran the 1% gel at 80 V for about forty minutes
 * <li> only primer bands showed after this time period
 * <li> it was suggested that phusion polymerase may not have been added before PCR, so 1 uL of phusion was added to the samples and phusion PCR was re-initiated
 * <li> following PCR, the results were run on a gel with the same results as above.