Minnesota/5 August 2008

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 * 1. Plasmid Prep: Plasmid prep from the 2mL cultures made from picked colonies of (a) TetR/p22mnt + RFP + Term, (b) Lac/LAMBDA + GFP 1, (c) Lac/LAMBDA + GFP 2, (d) Tetpro + LAMBDAcI + term.
 * 2. Spectrophotometry: Spec'ed the plasmid prep products to check for concentration of DNA.
 * 3. Digest Rxn: Run a digest reaction on the plasmid prep products. Follow the chart below:
 * 2. Spectrophotometry: Spec'ed the plasmid prep products to check for concentration of DNA.
 * 3. Digest Rxn: Run a digest reaction on the plasmid prep products. Follow the chart below:
 * 3. Digest Rxn: Run a digest reaction on the plasmid prep products. Follow the chart below:


 * 4. Vector Dephosphorylation of Digest Products: Dephosphorylated the BV, Lac/LAMBDA + GFP 1, and Lac/LAMBDA + GFP 2. Did not dephosphorylate the PCR products or the terminator. Used 1uL of antarctic phosphatase and 5.6uL of antarctic phosphatase buffer. 30 mins @ 37C in an incubator, then 15 minutes of heat inactivation.
 * 5. Run a gel on Digest Terminator Product: Using 1% agarose gel, check for correct amount/type of DNA bands. Only run terminator on gel. Will most likely not visualize band because terminator is approx. 40 bp's long.
 * 6. Gel purification: The terminator band was cut out of the electrophoresis gel and purified out using QIAGEN's QuickSpin gel purification protocol. DNA used in ligation below.
 * 7. Ligations: Ligated the digested, dephosphorylated, and gel purified products. After following the procedure/table of ligations below, we stored the ligated products @ -20C O/N to then transform tomorrow into competent cells. Folow the ligation table below:
 * 6. Gel purification: The terminator band was cut out of the electrophoresis gel and purified out using QIAGEN's QuickSpin gel purification protocol. DNA used in ligation below.
 * 7. Ligations: Ligated the digested, dephosphorylated, and gel purified products. After following the procedure/table of ligations below, we stored the ligated products @ -20C O/N to then transform tomorrow into competent cells. Folow the ligation table below:
 * 7. Ligations: Ligated the digested, dephosphorylated, and gel purified products. After following the procedure/table of ligations below, we stored the ligated products @ -20C O/N to then transform tomorrow into competent cells. Folow the ligation table below:
 * 7. Ligations: Ligated the digested, dephosphorylated, and gel purified products. After following the procedure/table of ligations below, we stored the ligated products @ -20C O/N to then transform tomorrow into competent cells. Folow the ligation table below:


 * 8. Sequencing Rxns: Send in sequences. Follow the mixture below, which was sent in today:
 * 8. Sequencing Rxns: Send in sequences. Follow the mixture below, which was sent in today: