Alberta NINT/24 June 2008

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lab work (SD): Transformation results: 2 colonies on K102008 plate and 2 colonies on K102008 negative control plate Redid ligation of K102002/B+N with TA0/B+N (1/100) and transformation into XL1-B cells. Made overnights of the colonies from 23/06/08 transformation. Made glycerol stock of K102002.

lab work (JD): Digested K102003 with BamHI and NcoI and incubated for 2 hrs at 37C Ran gel slowly on 2% agarose Cut out desired fragment and gel purified Counted and recorded colonies from yesterdays ligation: Negative       200 K102007        200 From results it seems ligation didn't work. Quantified K102003/B+N.