Team:NTU-Singapore/Notebook/25 July 2008



  

=Friday 25 July= {|border="1" style="background-color:#ffffcc;" cellpadding="20" Heat inactivation at 80 celcius in thermal cycler before gel run. Lysis standard vector is successful, showed a band between 100bp and 200bp(140bp) LsrA standard vector failed. Lysis Terminator was kept in the fridge for saturday gel run due to time constraits i had on friday.
 * Darius
 * Miniprep 23 tubes of bacteria, E7-ter, Lysis Terminator, lysis, lsrA respectively.
 * Digestion of E7-terminator, lysis Terminator, Lysis Standard Vector, LsrA standard vector.
 * Digestion of E7-terminator, lysis Terminator, Lysis Standard Vector, LsrA standard vector.
 * Run Gel Check
 * E7 showed a 2k Band, however, this is not conclusive as GFP vector and E7 Is of the same size.


 * PCR verification of E7 terminator. Ran PCR with different constituents.
 * Old E7 Primers, New E7 primers, E7 verification primers.
 * Results seen on saturday morning.