Team:NTU-Singapore/Notebook/30 June 2008



   =Monday 30 June:= {|border="1" style="background-color:#ffffcc;" cellpadding="20"

Chin Chong

 * Stock check of items purchased so far and shifted them into new lab with Darius's help
 * Received quotation for LsrA-RBS and Lysis sequencing from Science Werke, confirmed the purchase and sequence should arrive in 2-4 weeks
 * Purchased items from SBS general store
 * Did inoculation of colonies from Top cells containing:
 * 1) LacI-GFP BBa_I763004,
 * 2) Standard promoter with GFP reporter BBa_I20260
 * 3) GFP reporter devices with Weak BBa_J23150,
 * 4) Medium BBa_J23151 and
 * 5) Strong BBa_J23102 promoters
 * Incubate cells in 5ml of LB broth containing the respective anitbiotics and allow them to grow overnight
 * This is the slight modification from the standard protocol suggested in the Newsletter Vol.1 which used supplemented M9 medium instead of LB broth
 * M9 medium with glycerol will be introduced in the following day after the cells have been allowed to grow overnight in LB

Choon Kit, Zhen Fu,Hung:

 * Nanodrop to measure concentrations and purities of pFe,pLacI,GFP,RBS32,RBS34,pT7,Terminator. The data show a ratio of A260/A280 varying around 1.85 to 1.95, which mean the purities are quite good.