File:Jr pulsegen 3.jpg

A 1% agarose gel of XbaI/PstI test digests of plasmids holding the lac generator behind the tet-controlled promotor (RL1, RL2, RL3) and of XbaI/PstI test digests of plasmids holding the constitutive tetR expression cassette behind the promotorless lacI generator (TL1, TL2, TL3). The insert size agrees with the expected size in the case of RL1, RL2, RL3, TL1, TL2. TL3 apparently religated despite dephosphorylation treatment with alkaline phosphatase and does not contain the tetR expression cassette.

B 1% agarose gel of XbaI/SpeI test digests of assembled pulse generator precursor. Insert size conforms with the expected size in all cases.