Team:WEGO Taipei/Project

Overall project
Cancer cell is a kind of cell that its DNA has some damages, and those damages let it gain some different features with our normal somatic cell. Is is because its high replicated rate and its defects in cell cycle checking point, we start to think that if we put a antibiotic gene into a cancer cells, will the gene mutant rapidly? This is the begin of this experiment. The final goal of this experiment is to build a cancer cell's cell line that can produce antibiotic in vitro and secrete the antibiotic to its medium for us to retrieve it. By doing that, we might can produce new antibiotic easily and efficiently.

The Experiments
Following are the steps of our experiment:

[Preparation]

1. Geting the thing we need (such as cancer cells, E. coli and antibiotic gene). 2. Registering the proper labotory. 3. Making a realistic plan. 4. Making sure the detail of the experiment and making form for them.

[Experiment]

1. Making a antibiotic resisted E. coli. 2. Send antibiotic gene into the cancer cells. 3. Test if the gene will be perform and secrete outside the cells. 4. Test the mutant rate of the antibiotic gene. 5. Culture cancer cells and E. coli in the same plate.