Team:Chiba/Calendar-Home/18 October 2008

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17 October 2008 <|> 19 October 2008

Team:Demo-Rs
17 October~
 * 1) Sender Wash
 * 2) Centrifuged 2 tubes containing(BBa_T9002(JW1908))at 20&deg;C,3600rpm for 6min and discarded supernatant.
 * 3) Added 10mL LB-Amp to each tube.
 * 4) Repeated wash twice.
 * 5) Creating bacterial plates
 * 6) LB-Amp pre-cultured Sender(BBa_S03623(JW1908)) tube 1 (10mL) was mixed with LB-Amp-agar(50&deg;C)(10ml)to produce sender containing bacterialplate-1.
 * 7) Lifted with nitrocellulose
 * 8) Receiver(BBa_T9002(JW1908))colony was transfered to a nitrocellulose filter and placed on each of Sender(BBa_S03623(JW1908))containing bacterial plate (1~3) and Sender-absent negative control plate (t=0). Determined the time required for the colonies to fluoresce depending on the bacterial concentration (100 and 1000-fold dilution).
 * 9) Method to detect fluorescence
 * 10) Plates cultured at 37&deg;C were exposed to UV (312nm) light once every 30 minutes to observe GFP fluorescence.

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