Team:Hawaii/Construction of pRL1383a

Ligation of Parts

 * The BioBrick parts of pRL1383a are to be ligated in a series of experiments.

Methods

 * 1) Round 1 of Restriction Digest and Ligation


 * 1) In gel purification of ligation products


 * 1) Round 2 of Restriction Digest and Ligation


 * 1) Transformation


 * 1) Verification with Colony PCR

Discussion

 * What was learned and how to do future experiments differently.