Team:Hawaii/Notebook/2008-08- 2

= Things we did today =

PCR

 * Grace


 * Colony PCR of nir-1, nir-2, GFPf-1, GFPf-2
 * Annealed at 62C; extended for 1 min.
 * Used all new PCR water, primer solution, and Green Taq
 * Cut out bands for nir-1, GFP-1 (correct size)
 * PCR of PCR components to test for contamination
 * New primer solution + new PCR water + old Taq = no bands
 * Old primer solution + new PCR water + new Taq = band at 300bp = primer solution was contaminated
 * New primer solution + old PCR water + new Taq = bands at 270bp and 350bp = PCR water was contaminated



Cryostocked

 * Grace


 * nir-1, GFPf-1
 * Threw out old (incorrect) cryostocks of nir and GFPf

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"