User:University of Washington/25 July 2008

LuxR from AraC and TetR
-Received primers to biobrick Elowitz's plasmid.

-Did PCR amplification on Elowitz's plasmid (from 4 minipreps).


 * Reaction Set-Up
 * Thermocycling

- Good news. Ran gel on PCR product. Expected 172 bp. Got fragments shorter than 500 kb. =]

- Bad news. Sequencing result for second trial Quikchange came. The sequences did not show any mutation. =[

LuxR from pLac
-R0010+E0420 transformed cells' DNA was sent in for sequencing.

-Glycerol stock of DH5a+lacq strain made.

-One aliquot of electrocompetent DH5a+lacq cells were made.

-Sequence of part I0462 from the 2007 plates was received back and found to be the incorrect sequence. Sent an email to igem hq requesting bacterial stab of part I0462.

Back to Team:University_of_Washington/Notebook