User:University of Washington/14 August 2008

LuxR from AraC and TetR(Faifan)
QuikChange Approach

- miniprepped QuikChange overnight cultures (rxn1 x 4, rxn2) and submit sequencing:Forward

Elowitz

-dilute 1:10 of digested BBa_Elowitz

-3 way ligation(20 ul rxn)
 * mix
 * 30 mins incubate, room temp
 * 10 mins denature, 65 degree Celsius
 * 20 mins filtration

-transformed into XL1-Blue and plated on Kan

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