Alberta NINT/3 June 2008

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lab work (SD):

Ran K102002/B+N and K102004/B+N on 2% agarose gel to purify. Used QIAquick nucleotide removal standard protocol to purify T/A 0. Ligated T/A 0 into K102002/B+N and K102004/B+N.



lab work (WM): #colonies K102011:   48           negative ctl:  0 Picked 4 colonies for O/Ns