Team:Chiba/Calendar-Home/10 September 2008

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9 September 2008 <|> 11 September 2008

Team:Input
no work

Team:Communication
(9/9)-->Colony Count
 * 1) LuxI(BBa_K084012)
 * 2) LuxI+LVA(BBa_K084014)
 * 3) LasI(BBa_K084007)
 * 4) Background(R0010)
 * 5) Background(BBa_C0261)

Pick colony

Colony-PCR
 * Colony PCR of 10 colonies from ligation plate(9/9-(2)) and 3 colonies from ligation plate(9/9-(3)) one from control plate(BBa_F2620(2007)).




 * 95&deg;C,5min -> ( 95&deg;C,1min -> 52&deg;C,1min -> 72&deg;C,1min )･･･25cycles -> 72&deg;C,10min -> 6&deg;C

-->Gel Check

--->(28/8)Miniprep

eluted with 50&mu;L of TE buffer.

Team:Output
TIME Responce(liquid)

TIME Responce(solid)

Colony PCR

-->Gel check

-->Mini prep