Team:Hawaii/Notebook/2008-07- 4

= Things we did today =

Hybridized and ligated nir, slr2016, pilA synthesis oligos

 * Grace & Krystle


 * Redid yesterday's hybridization and ligation
 * Used 10-6 dilutions of annealed products for agarose gel run and in ligations
 * Combined 1 &mu;l each part, 7 &mu;l nanopure water, 10 &mu;l 2x Quick ligation mixture, and 1 &mu;l Quick T4 ligase for ligation reaction. Incubated one set at room temperature, another at 40C for comparison.
 * Checked hybridization and ligation on 4% agarose gel (95V, 28 min.)
 * Gel turned out blank. Did not add enough DNA (used 10-6 dilution). Redo calculations and run gel again on Monday.

Reorganized Biobrick and plasmid cryostocks

 * Grace & Krystle

= Discussion =
 * For inventory listing, see Inventory

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"