Team:NTU-Singapore/Notebook/15 August 2008



  

=Friday 15 August= {|border="1" style="background-color:#ffffcc;" cellpadding="20" Ligation of:
 * 1) E7(I)-Terminator(V)
 * 2) LsrA(V)-Lysis(I)
 * 3) Lysis(V)-Terminator(I)
 * 900-930:Hung: digestion of E7-I(E/S), Terminator-V(E/X), GFP (E/S) to compare the bands with E7, LsrA-V(S/P), Lysis-I (X/P), Lysis-V(S/P), Terminator-I(X/P). Incubation for 2.5 hours.
 * 1200-1230: Lu Chao: Minelute PCR purification.
 * 1230-1430: Lu Chao: gel loading+running+staining.
 * 1430-1600: gel extraction + nanodrops. All the bands needed were shown under UV!
 * 1600-1645: ligation.
 * 1645-1715: MinElute for ligation mixtures.
 * 1715-1900: transformation into Top10.