Alberta NINT/4 June 2008

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lab work (SD):

Transformed XL1-B cells with plasmids K102008, K102009 and negative controls.

lab work (JD):

From sequencing results K102001.1 becomes the K102001. Digested K102001 with SpeI and PstI. Ran gel electrophoresis and cut out desired band ~2500bp

lab work (WM): Miniprep 4 O/Ns of K102011 transformants (2a, 2b, 3a, 3b)