EPF-Lausanne/21 July 2008

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Transformation of Friday : Results : The control worked well. One of the part, who had been treated with elution buffer instead of TE, had a few colonies (about 10). This part, treated with TE, had also 4-5 colonies but all at the same place... The other part had no colonies, whichever buffer we had used. We put 8 colonies in tube with 3 ml of LB + antibiotic and let them grow overnight at 37 degrees.

We tried to make a new transformation with 2 random parts in TE and in an other buffer(elution buffer from a miniprep kit). We followed the iGEM protocol, but this time, we incubated the DNA in the buffer for 20 minutes at 42degrees. We also tried to make an other transformation with 2 other random parts in 10 microliter of TE or elution buffer.

As our transformations didn't work, we worked on the design of primers for different parts we need. They have been ordered this afternoon.

We also worked on the design of our final plasmid.