Alberta NINT/18 July 2008

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lab work (SD): The double-plasmid cell cultures showed one-two colonies on the LB+amp+kan35 plates. To test transformation efficiency, we will plate 150ul of XL1-B with pSB4A5+pSB4K5 on LB+amp50, LB+kan35, and LB+amp50+kan35 plates using different amounts of plasmid and cells. pSB4A5 (ul)  pSB4K5 (ul)   XL1-B (ul) A             1.0           1.0           100 B             4.0           4.0           100 C             1.0           1.0            50 D             2.0           2.0            50

lab work (JD): Ligations seem to have worked: K102031: 1 K102021: 3 Negative: 0 Made O/Ns from these colonies Replated cells left at the bottom of tubes from yesterday's transformation