Team:Chiba/Calendar-Home/3 September 2008

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2 September 2008 <|> 4 September 2008

Team:Input
no work

Team:Communication

 * (2/9)--->Mini prep
 * BBa_K084009(R1, R3~R7)
 * BBa_K084010(C1,C2,C4~C8)
 * BBa_S03154


 * --->Gel Check


 * --->Digestion test
 * BBa_K084009(R1, R3~R7)
 * BBa_K084010(C1,C2,C4~C8)


 * --->Gel Check


 * (2/9)--->Phenotype-test
 * MIX
 * BBa_K084007(Plac+RBS+LasI, Competent Cells : JW1908) -> Sample Name : L1~L4
 * BBa_K084008(Plac+RBS+RhlI, Competent Cells : JW1908) -> Sample Name : R1~R4
 * BBa_S03623(Ptet+RBS+LuxI, Competent Cells : JW1908)
 * BBa_T9002(Ptet+RBS+LuxR+GFP, Competent Cells : JW1908)




 * Incubated for 8hours at 37 degrees
 * Spindown (max rpm, 3 min)
 * measured the intensity GFP(visual judgment)


 * Left for 12 at room temperture.
 * Resuspensioned
 * measured the intensity of GFP by Fluoroskan Ascent 2.5(program:Ascent Software Version 2.6)

Team:Output
Digestion test
 * BBa_R0079(1)
 * BBa_R0071(2)
 * BBa_R0077(3)
 * BBa_R0078(4)
 * BBa_R0062(5)

-->37&deg;C30min

-->Gel check