Team:Hawaii/Notebook/2008-09- 5

= Things we did today =

Construction of Broad-Host-Range Vector Intermediates

 * Margaret


 * Plasmid Prep: Everything that didn't work yesterday
 * 2 Plac + B0030, 1 Plac + B0034, aadA(BB)colony 5, pSB3k3, pSB1A3
 * Restriction Digest: same as Yesterday
 * Ligation
 * Transformation into DH5alpha (we need more competent cells)

Verification of transformants from yesterday

 * Grace


 * BBpRL1338a related plates all had a lawn (no distinct colonies observed). Too low antibiotic conc.? Will attempt to restreak/replate on Mon.
 * Colony PCR of 3A assembly related colonies
 * Oops. Loaded 1 &mu;l colony #11 into colony #10's well
 * Colony #10 has potential. Will restreak on Mon.

Sequencing

 * Margaret


 * Analysis of Sequencing submitted (oriV1-4, 2 aadA(BB), 2 aadA (pRL))


 * Grace


 * Confirmed:
 * nir+rbs
 * rbs+GFP


 * YAY!

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"