Rensselaer/7 July 2008

Overviewed procedure for introducing gene of interest:

pick plasmid filter paper - into buffer

primer design - insert restriction site (comparable with plasmid)

PCR (primers, DNA polymerase, dNTP's,buffer)

Digestion w/ restriction enzymes

Ligation (plasmid and insert)

introduce to bacteria (transformation)

potential problem - psuedomonas - how to get plasmid DNA?

perform PCR on solution