Team:Warsaw/Calendar-Main/16 July 2008

Cloning omega-A fusion on pKS (second attempt) Michał L., Ewa, Marcin We have massive outbreak of some virulent bacteriophage strain in our lab. All our current E. coli cultures are gone :-(. Until we get rid of the phage there will be no microbiological work in our lab. Sorry.

Cloning of protein Z DNA to OmpA constructs Michał K.    Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_Z_omega).   Control digest of isolated plasmids with BamHI and SacI (BamHI buffer).  Gel electrophoresis - both checked colones are good (Fig. 1.).</li> </ol>

<img src="http://2008.igem.org/wiki/images/a/ac/Dwa.jpg" width=300/></a> Fig. 1. Control SacI/BamHI digests of isolated plasmids 1. Marker 2-3. digested plasmids pACYC177+OmpA_Z_omega