Team:Hawaii/Meeting/2008-09- 8

Agenda

 * 1) Exchange Lab Techniques & Protocols
 * 2) plasmid prep using low media volume, big flask (Margaret)
 * 3) SAP treatment of vector to prevent self-ligation, success? (Grace/Krystle)
 * 4) Gel cutting using SYBRSafe+Blue Light, Yield Loss at each step(NW)
 * 5) 70min PNK+Oligo Annealing Protocol (NW)
 * 6) Updates of progress from the past two weeks
 * 7) Grace
 * 8) Re-replacement of BB-pRL1383a MCS
 * 9) Krystle
 * 10) GFPf w/ tt? GFP w/ tt?
 * 11) Margaret
 * 12) Rep, OriT, OriV, etc...
 * 13) aadA, Omega
 * 14) P1 Lytic
 * 15) Norman
 * 16) Bioplastic synthase operon (phaABCE) construction progress
 * 17) site directed mutagenesis of two genes phaE and "sigma master switch"
 * 18) Logistics
 * 19) Travel itinerary (NW)
 * 20) Sign Waiver (All)

Minutes
Present: &lt;Person A&gt;, &lt;Person B&gt;



Action Items

 * &lt;Person A&gt;: Task