TUDelft/22 October 2008

=October 22nd=

Luciferase & Protein Content: Bead Beater style
Today we've done a new luciferase assay using the beadbeater. Results of BCA (protein content) are displayed in figure 1.



Corrected luciferase measurements are displayed in figure 2.



We've done some luciferase measurements also using the old protocol with lysis buffer. These are displayed in figure 3, but aren't corrected for protein content but for OD.



Miniprep
Three different vectors were prepped with a Qiagen kit according to manufacturer's protocol. These three didn't yield a sufficient DNA concentration yesterday. Today after prepping these were fine and so all vectors are ready to be sequenced and also ready to be sent to Boston.