Alberta NINT/16 July 2008

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lab work (SD): Since the double-plasmid cell cultures failed to grow on the LB+amp50+kan35 plates we will try to             grow them on LB+kan35. Each of the plasmids is also transformed alone into XL1-B cells and plated on kan35 plates as well. K102022 (LB+amp50) K102032 (LB+amp50) K102032+K102011 (LB+kan35) K102032+K102034 (LB+kan35) K102032+K102035 (LB+kan35) K102032+K102036 (LB+kan35) K102011 (LB+kan35) K102034 (LB+kan35) K102035 (LB+kan35) K102036 (LB+kan35)

lab work (JD): Mini-prep of pSB4A5, K102003, K102006 Restriction digests: pSB4A5/E+P, K102003/E+P and K102006/E+P Ran gel electrophoresis, 2% gel, 100V