Team:Harvard/Recipes

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{|align="justify" style="background-color:#FFFFFF;text-indent: 15pt;text-align:justify" cellpadding="50" width="90%" =Recipes= We experimented with several types of growth media for Shewanella growth, both aerobically and anaerobically. The most effective growth media recipes are listed as follows.

Orianna's growth media

 * 50 mM K-PIPES             18.925 g/L
 * 7.5 mM NaOH               7.5 mL/L
 * 28 mM NH4Cl    1.5 g/L
 * 1.3 mM KCl                433 uL/L
 * 4.3 mM NaH2PO4 0.59 g/L
 * 100 mM NaCl 5.84 g/L
 * 18 mM Lactate 1.62 g/L
 * 10 mL Vitamin solution
 * 10 mL Amino acid solution
 * 10 mL Trace mineral solution

LML medium (for anaerobic Shewanella growth)

 * 0.02% yeast extract
 * 0.01% peptone
 * 20 mM lactate
 * 10 mM HEPES [pH 7.4]

LML agar (for Shewanella reduction test)

 * LML medium
 * 0.8% agar
 * 2 mM MnO2- Mn(IV) oxide (turns from brown to clear upon reduction to Mn(II))

(LML recipes from | Beliaev and Saffarini 1998)

Chamber media
7.0 pH
 * 5.844 g/L 100mM NaCl
 * 15.1185 g/L 50mM PIPES (hydrogen)

Phosphate buffer
7.0 pH
 * 2.918 g/L Monosodium phosphate, monohydrate
 * 4.095 g/L Disodium phosphate, anhydrous
 * 5.844 g/L 100mM NaCl

Lactate solution

 * 13.51 g/L Lactic acid
 * neutralize to pH 7 with NaOH


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