Team:Paris/August 23

=Construction for FIFO=

Aim : Construction of pFlgA - YFP tripart (+/- LVA) "pFlgA-RBS-YFP-dbl ter" (pFlgA-E0430/E0432)

Results of the transformation we did Yesterday
=> Need to screen to know which clones we can use for the of  pFlgA promotor cconstruction.

=Construction of pFlgA - GFP Generator=

Aim : Construction of  "pFlgA-RBS-GFP-dbl ter" (pFlgA-E0240)

Transformation of the ligations we did yesterday
Protocol

=Construction for Synchronization=

Number of colonies
=> We decided to do again the digestions of the ligation's reactions

Digestion
Protocol

=Promoter characterization plasmids=

Transformation results: ligations from August 21th
Top 10 cells were used

Protocol