Team:Hawaii/Experiment

Experiment Template

In Progress

 * Ligation of pRL1383a Parts
 * Construction of Broad-Host-Range Expression Vector
 * Construction of Omega Interposon BioBrick
 * Antibiotic test for aadA construct
 * Conjugation Test: Comparing oriT with oriTr

Not Started

 * Electroporation of PCC6803

Completed

 * PCR Amplification of pRL1383a
 * PCC6803 cell density test
 * PCC6803 Cryostock Thaw Test
 * Effect of CO2 on Growth Experiment
 * Effect of Water Motion on Clumping of Liquid Cultures
 * Make Competent E. Coli
 * Test Competent E. Coli
 * Initial E. Coli Transformation
 * Initial BioBrick Plasmid Extraction From Filter Paper
 * Biobricks extraction/transformation competency test
 * Initial PCC6803 Transformation
 * Tri-parental conjugation between E. coli and PCC6803
 * Mini Plasmid Prep
 * Large-Scale Preparation of Plasmid
 * Initial Synthetic Oligonucleotide Assembly
 * Conversion of GFP into a fusion brick and pRL1383a into a Biobrick vector
 * Antibiotic test for BB-pRL1383a