Team:Warsaw/Calendar-Main/1 July 2008

Change of the reporter from pZC320 with B-galactosidase to GFP or RFP Piotr, Weronika  Isolation of plasmids from transformants. Control  digest of plasmids with NotI. Gel electrophoresis of digested DNA - there where no proper plasmids.

Isolation of pZC320.</li> Isolation of pSB1A2</a> standard plasmids carrying parts: <A href=http://partsregistry.org/wiki/index.php?title=Part:BBa_E0840>BBa_E0840</a>(GFP genetrator) and BBa_J04450</a> (RFP generator).</li>

Overnight digest of pSB1A2</a> standard plasmids carrying parts: <A href=http://partsregistry.org/wiki/index.php?title=Part:BBa_E0840>BBa_E0840</a>(GFP genetrator) and BBa_J04450</a> (RFP generator) with NotI.</li>

 Overnight digest of pZC320</a> with NotI and dephosphorylation</a> with CIAP. </li></ol>

Cloning alpha-A and omega-A fusions on pKS</a> Michał L., Ewa, Marcin <ol> Isolation</a> of plasmids from transformants.</li> Control  digest</a> of plasmids with SacI+NotI (BamHI buffer).</li> Gel electrophoresis of digested DNA.</li>

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We have successfully cloned A-alpha and A-omega fusions on pKS</a> vector. The DNA will be now sequenced to ensure that our constructs are correct.

Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega Paweł <ol> <li> <a href="http://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of <a href=http://www.emdbiosciences.com/docs/docs/PROT/TB045.pdf>pET15b</a> plasmid.</li> <li> Overnight <a href="http://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of <a href=http://www.emdbiosciences.com/docs/docs/PROT/TB045.pdf>pET15b</a> plasmid with NdeI and BamHI (Tango 2x buffer), <a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation with CIAP</a>.</li></ol>