Beijing Normal/21 July 2008

Results: The transformation of pSB1A3 is unsuccessful, however the situation of pSB1AT3 is no better: just 20~30 colonies. The steps as follows: 1.cut douwn the sports stricktly following the instructions provided and we do spin it. 2. add all the TE containing the plasmids to 50ul competence cell (Top10, tested before expet) 3 stock in the 4 degree frigde for 30mins 4 42 degree heat shock 5 4 degree stock for 5~6 mins 6 add 300ul SOB. 7 37 degree incubate for more than 60mins 8 plate the incubated cell culture on the plate containing amp 9 incubate the plate at 37 degree for 12~14h

Questions: However, we wanna to ask other teams members: 1 When u eject the sport into the tube, will the colour turn pink? 2 Is 5ul TE enough for u to elute the plamids? Or the solution is all absorbed by the the soaked paper? 3 What does the instruction mean by 10% bleach? We have several kinds of bleach in the lab and are confused about which is proper.