Team:Warsaw/Calendar-Main/10 September 2008

Tests for ampicillin resistance given by protein added to medium Piotr, Emila

Inoculation of all pACYC177+OmpA variants from previous day to LB + 0.25 mM IPTG, kanamycin, ampicillin (50 μg/ml) and various concentration of protein (A-alpha, Z-alpha and Z-omega; negative control without protein).

 Inoculation of pACYC177+OmpA_A_alpha, pACYC177+OmpA_A_omega, pACYC177+OmpA_Z_omega, pACYC177+OmpA_alpha and pACYC177+OmpA_omega to LB + 0.25 mM IPTG and kanamycin.

Measurement of growth after 6 hours.</li></ol>

<img src="http://2008.igem.org/wiki/images/6/65/Wykres-piotr.jpg"> Bacterial growth depending on the amount of prey protein added to the medium. The amount is expressed in picomoles per mL of medium. Z_omega: 20323.75 Da, Z_alpha: 28734.24 Da, A_alpha: 37052.21 Da.

Z_omega was added to bacterial culture expressing pACYC177+OmpA_A_alpha</a> Z_alpha was added to bacterial culture expressing pACYC177+OmpA_A_omega</a>. A_alpha was added to bacterial culture expressing pACYC177+OmpA_Z_omega</a>.