Team:Hawaii/Notebook/2008-06-12

= Things we did today =

Triparental conjugation

 * Grace


 * Began triparental conjugation between DH5&alpha; transformed with pRL1383a, E. coli with RP1, and PCC6803 (see experiment)
 * Innoculated BG-11 + 5% LB plates

Purified BBa_B0034 colonies

 * Krystle


 * Streaked out colonies onto fresh LB+amp100 plates

Preparation for making competent cells

 * Krystle & Grace


 * Autoclaved detergent-free erlenmeyer flasks
 * Innoculated 250 ml SOB w/ DH5&alpha; cryostock; shook overnight at 26C with shaking (150 rpm)

Made BG-11+sm+sp plates

 * Krystle


 * Made 20 plates
 * 2.5 &mu;g/ml each of sm and sp, labeled Sm2.5Sp2.5

Made SOB media

 * Krystle


 * 2 batches (and added antibiotic... oops! will autoclave again to get rid of it)

Made BG-11

 * Norman
 * stored in room temp incubator bottom shelf
 * will need to make bunch of 50 mL stock solution of TES and Thisulfate during some downtime.

Sequence synthesis

 * Grace


 * rbc promoter Biobrick sequence to be synthesized

Parts list

 * Norman
 * Parts list submitted by team members; Norman will send a request to iGEM for parts not on filter paper

Primer Design Review

 *  Norman 
 * will review some primers tonight before shelling out money to buy'm

Quote of the Day

 * "We like lab math, except when we have to divide by even numbers. We can't divide by even numbers therefore we can't do binary fissions." -GK, KS
 * "Bye" -GK, KS in synchronized tone...
 * "You girls should join a choir..." -GP
 * "You not only dress the same, you talk with the same voice at the same time, are you trying to confuse me again!?" -NW