Team:Illinois/Protocols

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General Molecular Biology
A great textbook called Current Protocols in Molecular Biology available entirely online contains protocols for pretty much any molecular biology technique- great resource.

Polymerase Chain Reaction, Plasmids, and Recombinant DNA
PCR is the foundation for everything we will do ever. Below are some links on the basic principles and practice of pcr and related important topics; as we begin our own protocols those specific steps will be recorded here.

I would start here for a nice intro to the historical context of PCR.

The wikipedia page on PCR is good; I would skip the sections on 'variants' and history. Also their page on cloning genes into cells is good, and subcloning, all of which we'll probably end up using. And BACs and YACs.

A good animation on basic PCR, and an awesome animation on plasmid methods.

Decent explanation and some examples of Recombinant DNA techniques. A very very well done silent film on same.

The wikipedia page on gel electrophoresis, and specifically agarose gel electrophoresis for DNA, which you use to resolve and purify PCR products.

Something trippy.

Something not quite as trippy.

Yeast Culturing
Longterm storage and streaking protocols

Growth and manipulation of yeast

Yeast culture media

Yeast transformation protocols

Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual (1998 edition available in Biology library, newer editions currently checked out)

Yeast Info on OWW Contains General, Protocols, Materials, and References Info - Alzar

E. coli Culturing
http://cgsc.biology.yale.edu/

[http://www.ciwemb.edu/labs/koshland/Protocols/BACTERIA/ecolicells.html Frozen Competent E.Coli Cells. Methods and protocol for freezing E.Coli Bacteria Competent Cells in aliquots for later use re-use.]

Opimization of recombitant E. coli protein expression in growth medium

E. coli gene expression protocols (book available online on google books)

General indications and protocols for the expression in bacteria

Glycerol storage of E. coli bacterial stocks

Different liquid culture media for E. coli

Different dry culture media for bacterial growth