Team:Imperial College/Protocols

Experiments & Protocols
{{Imperial/Box1|Protocols|

1. Transformation Protocols for integration vectors in Bacillus subtilis

 * Protocol 1
 * Protocol 2

2. Transformation Protocol for E.coli

 * Preparation of XL1-blue electrocompetent cells

3. PCR

 * PCR

4. Calibration Curves

 * Calibration Curves
 * O.D 600 vs cell number
 * Fluorescence vs GFPmut3b

5. Promoter-RBS Testing Protocols

 * Constitutive Promoters and RBS
 * pHyperspank Promoter and RBS
 * Light Inducible Promoters and RBS

6. Antibiotic Resistance Testing

 * Chloramphenicol Resistance Assay
 * }}