Team:Mexico-UNAM-IPN/Horizontal-Transfer/9 September 2008

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CONTINUES FROM THE SEPTEMBER 8 ENTRY

New antibiotic calculus

Ampicilin
 * Stock 100mg/ml
 * Lethal concentration 512µg/ml
 * 5.12µl from the stock have to be added to the media.

Tetracycline
 * Stock 5mg/ml
 * Lethal concentration 7.5µg/ml
 * 1.5µl from the stock have to be added to the media.


 * 1) 4µl of each overnight culture was taken with 5ml of LB. Incubation 3hrs at 37°C at 150rpm. Three controls were made and three with Tet. Each control corresponds to one strain.
 * 2) After 3 hours 7.5µl of the Tet stock were added to kill the bacteria population that should be in the exponential phase.