Waterloo/24 June 2008

08/06/24 Tuesday [x] streak/patch/innoculate 2007 and 2008 registry parts (13:00 - 14:00) [x] re-transform one sample from yesterday and PocI (13:00) - No growth [x] diagnostic digest of PluxRgfp" (1-8) with gfp`` positive control (repeat from 20/6), and with sketchy DNA from 23/6 digest (TT, CI, PtetR, PoCI, PlacoCI, Pcost)  (~1.5 hr) [x] gel (1.5hr) [x] analyze 07registry gel from yeserday - Bad ladder, some samples ran ok for extraction, but needs to repeat other ones with better control [x] prepare more LB tubes and LB agar [x] make frozen stocks (20uL) of : - PocI and for the following also prepare eluted DNA. - PcI (R0052-2-9G) - PrecA (J22106-3-12F) - TT (B0014-1-1A) innoculate after confirming with dignostic gel