Virginia/25 July 2008

Overnight Results

 * EVERYTHING WORKED! all ligations were successful thanks to the new T4 ligase
 * Also the E0840 plate from the day before now had colonies

Accomplished

 * Made small overnight broth of all assemblies
 * Broth of A30 (RFP + GFP) received 10ul of IPTG to induce pLac in I715039
 * Digested pSB1A10 and B1002 with EcoRI and PstI and ligated, transformed

Back to Notebook