Purdue/18 June 2008

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Lab Meeting

In attendance:


 * Craig Barcus
 * Janie Stine
 * Jessamine Osborne
 * David Jaroch
 * Dr. Rickus
 * Dr. Clase

Minutes
 * 1) Commercial concerns
 * 2) Encapsulate in something so could withstand pool chlorination? (e.g. Reverse Osmosis Filter)
 * 3) Acquiring DNA/starting labwork
 * 4) Need to check to make sure promoters do not need any other parts to start transcription of the genes (Jess)
 * 5) Talk to the registry about gettting the lycopene gene (Part I742120/I742136)
 * 6) Make/order phr promoter (check prices or synthesize via PCR)
 * 7) Talk to Dr. Sanca about any DNA he has...
 * 8) TO DO THIS WEEK:
 * 9) Check promoters
 * 10) Figure out how to get lycopene and/or phr promoter DNA
 * 11) Talk to Dr. Sanca about K-12's natural promoters (either of ours taken out?) and/or see if he has anything he'd like to donate
 * 12) Check about ordering synthesized genes
 * 13) Start labwork!!!!! (Monday with Dr. Clase)
 * 14) Get competent cells (or make them)
 * 15) Transform with LacZ and/or SOS promoter
 * 16) Make glycerol stocks?
 * 17) Start attempting to make part: SOS + beta-gal

Information about parts to be transformed:
 * LacZ: Part I732005, Well 6F, plate 1018, plasmid pSB1AK3 (Kan/Amp resistant)
 * SOS promoter: Part J22106, Well 12F, plate 1003, plasmid pSB1A2 (AmpR)

Edited by Janie Stine