Team:Mexico-UNAM-IPN/Horizontal-Transfer/19 September 2008

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6.Stocks of diluted tetracicline (100µl witha a concentration of 5µg/ml) were made on august 28. Stocks in tubes of 50ml, 15ml and 1ml were made and kept at -80°C.
 * 1) The material was sterilazed: Tip containers, LB agar media, LB media, CaCl 2 solution, falcon tubes, eppendorf tubes, etc...
 * 2) Plates were prepared with LB agar (stocks of bacterial strains) and LB plates with antibiotic.
 * 3) To prepare the stock solution of CaCl 2 1M add 4.44g of CaCl 2 to 40ml od distilled water.
 * 4) Agar plates with lethal dosis of Amp and Tet.
 * Amp lethal dosis:512µg/ml
 * Tet lethal dosis:7.5µg/ml