Imperial College/12 August 2008

= 12 August 2008 =

B.subtilis

 * Mini prep of 5ml E.coli cultures containing the pDR110/pDR111
 * Competent B.subtilis prepared

PCR Cloning

 * Designed Primers for PCR cloning of XylR gene and EpsE integration sequences from the B.subtilis chromosome


 * Designed Primers for PCR cloning of AmyE integration sequences and Spectinomycin resistance from plasmid pDR111

Dry Lab

 * wrote a code on matlab to obtain velocity, direction, run time and tumble time from the positions of the cell at each frame
 * Did analysis on exponential distribution, plotting of Posterior of parameter lambda in response to obtained synthetic data samples generated.