Team:Warsaw/Calendar-Main/26 June 2008

Change of the reporter from pZC320 with B-galactosidase to GFP or RFP Piotr, Weronika  Isolation of pZC30.

Inoculation of E.coli TOP10 carrying pSB1A2 standard plasmids carrying parts: BBa_E0840(GFP genetrator) and BBa_J04450 (RFP generator).



Preparation of constructs: OmpA_alpha and OmpA_omega Michał K.

Repetition of PCRs, gel-out isolations and digests from <a href="http://2008.igem.org/Team:Warsaw/Calendar-Main/17_June_2008">17 June.</a>

Preparation of alpha-A and omega-A fusions Michał L., Ewa, Marcin <a href="http://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcl">Polymerase Chain Ligation (PCL)</a>

PCL program for fusion A with alpha and omega