Waterloo/14 August 2008

8/08/14 Thurs

[x] send gene 6 for sequencing

[] p/s/i of c0052', c0051', T7 gene 6 + RBS

[x] miniprep of inoculatants from wed

[x] Gel of overnight PCR of phbA and phbB [] if PCR didn't work - keep testing PCR conditions - PCR worked

[] pjet gene 5 ligation [] pjet gene 5 transformation [x] check gel of pjet extractions gene 5 - note: PCR worked, but no DNA was seen from minprep samples [x] PCR - PHB testing again.

[x] colony PCR condition testing [x] patch colonies from transformation