Team:MIT/Notebook

Procedural Daily Notebook

 * Click on any day below to see what wet-lab procedures were conducted.

Organizational Daily Notebook

 * Please see our daily notebook for a detailed daily summary of our daily meetings, brainstorming, discussions, and progress.

Information

 * Primers/Plasmids/PCR sequences
 * Lactobacillus delbrueckii subsp. bulgaricus Information & Protocols
 * Vocabulary List

Protocols

 * DNA Ligations
 * Top10 chem competent cell transformation
 * BLR electrocompetent cell transformation
 * DNA sequencing
 * PCR
 * Running DNA Gels
 * Restriction Digests
 * Preparing Antibiotic Stocks
 * Protein Purification
 * Novel Tooth Binding Assay
 * Lactococcus lactis Transformation Protocol
 * Colony PCR

Data

 * Toothbinding assay

Useful Links

 * DNA Plasmid Editor
 * Peptide Sequence Manipulations
 * Primer Analyzer Tool (check temperature & GC content) | One | Two
 * PMID 9920267 A synthetic peptide adhesion epitope as a novel antimicrobial agent.
 * PMID 9062560 Inhibition of Streptococcus mutans adsorption to hydroxyapatite by low-molecular-weight chitosans.
 * PMID 4055036 Albumin as a blocking agent in studies of streptococcal adsorption to experimental salivary pellicles.
 * PMID 12788739 Determination of the domain of the Lactobacillus delbrueckii subsp. bulgaricus cell surface proteinase PrtB involved in attachment to the cell wall after heterologous expression of the prtB gene in Lactococcus lactis. (a secretion signal sequence described; can use the promoter, RBS and secretion signal for this protein for p1025).
 * PMID 11772607 Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids.
 * High efficiency electrotransformation of Lactococcus lactis spp. lactis cells pretreated with lithium acetate and dithiothreitol