Team:ESBS-Strasbourg/8 October 2008

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WetLab
Katja:   results of the last two days: We have  So further assemblies will be:
 * mCherry_L_Vp16_L_lexA_L
 * CFP_cin8_adh1term (Manuels work)
 * Gal1_CFP_NLS_adh1term
 * Gal1_CFP_hsl1_adhterm
 * Gal1_mCherrry_L_lexA_L_VP16_L
 * Gal1 (BV - in the freezer) + mCherry_L_Vp16_L_lexA_L (BI - to be done)
 * Gal1 (BV - in the freezer) + lexA_L_Vp16_L_mCherry_L (BI - to be done)
 * Gal1 (BV - in the freezer) + CFP_cln2_adhterm (BI - to be remake)
 * Gal1 (BV - in the freezer) + CFP_cin8-adhterm (BI - to be done)
 * Gal1_mCherry_L_lexA_L_Vp16_L (FI - to be done) + NLS_adhterm (FV - to be done)
 * Gal1_mCherry_L_lexA_L_Vp16_L (BV - to be done) + NLS_adhterm (BI - to be done) (for savety)


 * Leu2 (BI) in pSB1A2 (X+P)
 * Ura3 (FI) in pSB1A2 (E+S)

General
Katja: I ordered primers for the Mutagenesis of Leu2 (silent mutation) and Ura3 (in non coding region) so that we can deleted the EcoRI and Pst1 site respectively. According to Mr. Chatton there shouldn't be a problem with transcriptional/translational regulation at this point.