Team:Chiba/Calendar-Home/27 August 2008

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26 August 2008 <|> 28 August 2008

Team:Input
no work

Team:Communication
Digestion Test
 * Sample DNA :BBa_R0010


 * --->Gel Check


 * (26/8)--->Colony-PCR
 * Colony PCR of 5 colonies from ligation plates (26/8-(4),(5)) and one from control plate(BBa_F2620(2007)).




 * 95°C,5min -> ( 95°C,1min -> 52°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C


 * --->Gel Check


 * --->(28/8)Miniprep

Transformation
 * competent cells : XL10G
 * BBa_C0062(2007)
 * BBa_C0062(2006) -> no colony on plates
 * BBa_C0179(2007)
 * BBa_C0179(2006)
 * BBa_S03156(2007)
 * BBa_S03156(2006)
 * BBa_S03158(2007)
 * BBa_S03158(2006)
 * BBa_S03160(2007)
 * BBa_S0316(2006)


 * --->Mini prep


 * --->(29/8)Gel Check

Team:Output
no work