Team:Hawaii/Notebook/2008-10-13

= Things we did today =

Verification of transformants

 * Grace


 * Colony PCR of restreak plates
 * Bands ~600bp. Plasmid did not cut.


 * Krystle
 * RE digest of plasmid w/ EcoRI and PstI
 * RE digest of prpgt, rgt1 plasmids with EcoRI and PstI
 * Ran RE digests on gel
 * Bands did not correspond to what was supposedly loaded


 * Grace
 * PCR of prpgt #5, 7, 11, nrsg #6, rgt #1
 * Overnight RE digest of devices with EcoRI and PstI and rgt#1 with XbaI and PstI

Triparental Conjugation

 * Grace
 * Restreaked 5%LB+BG-11 plates onto BG-11+sp2.5+sm2.5 plates for isolation of cyanos
 * Inoculated BG-11+sp2.5+sm2.5 liquid media with conjugated E. coli/cyanos
 * Incubated at 37C with 2% CO2 for 11 days. Liquid cultures were shaken at 100 rpm also.

= Discussion =

= Quote of the Day = "History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson"