User:University of Washington/10 July 2008

From 2008.igem.org

(Difference between revisions)
(non-RP4 Conjugation)
(RP4 Conjugation)
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== RP4 Conjugation ==
== RP4 Conjugation ==
-
'''Conjugation protocol #1'''<br\>
+
'''[[Protocols| Conjugation protocol #1]]'''<br\>
·DH5α <br\>
·DH5α <br\>
·DH5α + S17-1 w/ RP4 <br\>
·DH5α + S17-1 w/ RP4 <br\>

Revision as of 00:22, 10 July 2008

RP4 Conjugation

Conjugation protocol #1
·DH5α
·DH5α + S17-1 w/ RP4
·DH5α + naked RP4 DNA
·DH5α + amp resistant RFP plasmid

Lambda Red

·Synthesis tetR insert for KorA and trbA replacement

non-RP4 Conjugation

Mini Prep & Glycerol Stock
·CV13(Yep13)
·pDPT51


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