User:University of Washington/9 July 2008

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(RP4 Conjugation)
 
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== RP4 Conjugation ==
== RP4 Conjugation ==
-
·Success!!!  Bacteria-Bacteria conjugation works
+
·Success!!!  Bacteria-Bacteria conjugation works<br\>
 +
·Received lambda red primers for RP4 manipulation
 +
 
 +
== LuxR from AraC and TetR ==
 +
 
 +
- Took AraC plasmid after thermo cycling out from the machine, stored in the fridge.
 +
 
 +
- Plated GFP generator (BBa_E0240: received from BioCircuit Lab) on LB Agar + Amp.
 +
 
 +
== BioBrick Promoter Measurements ==
 +
 
 +
- Overnight promoter-construct cultures were diluted 1:100, then incubated in a rotator for 3 hours.
 +
 
 +
- Using a 96-well plate reader, fluorescence and OD600 measurements were taken every five minutes for eight hours for the four promoter-construct cultures, an empty culture of TOP10 cells, M9 media, and M9+Kan.
 +
 
 +
==LuxR from pLac==
 +
 
 +
-Part I736004 sequence was confirmed.
 +
 
 +
-Overnight culture of I736004 transformed cells were started in M9 media.
 +
 
   
   
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Back to [[Team:University_of_Washington/Notebook#Notebook]]
Back to [[Team:University_of_Washington/Notebook#Notebook]]

Latest revision as of 23:40, 24 July 2008

Contents

RP4 Conjugation

·Success!!! Bacteria-Bacteria conjugation works
·Received lambda red primers for RP4 manipulation

LuxR from AraC and TetR

- Took AraC plasmid after thermo cycling out from the machine, stored in the fridge.

- Plated GFP generator (BBa_E0240: received from BioCircuit Lab) on LB Agar + Amp.

BioBrick Promoter Measurements

- Overnight promoter-construct cultures were diluted 1:100, then incubated in a rotator for 3 hours.

- Using a 96-well plate reader, fluorescence and OD600 measurements were taken every five minutes for eight hours for the four promoter-construct cultures, an empty culture of TOP10 cells, M9 media, and M9+Kan.

LuxR from pLac

-Part I736004 sequence was confirmed.

-Overnight culture of I736004 transformed cells were started in M9 media.



Back to Team:University_of_Washington/Notebook#Notebook