User:University of Washington/25 July 2008

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(Difference between revisions)
(→LuxR from AraC and TetR)
(→LuxR from AraC and TetR)
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*Reaction Set-Up
*Reaction Set-Up
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<table>
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<table border=0.5>
<tr>
<tr>
   <th>reagents</th><th>amount per reaction(ul)</th>
   <th>reagents</th><th>amount per reaction(ul)</th>
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   <td>DNA template</td><td align=center>2</td>
   <td>DNA template</td><td align=center>2</td>
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</table>
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</table border=0.5>
*Thermocycling
*Thermocycling
<table>
<table>

Revision as of 21:39, 25 July 2008

LuxR from AraC and TetR

-Received primers to biobrick Elowitz's plasmid.

-Did PCR amplification on Elowitz's plasmid.

  • Reaction Set-Up
reagentsamount per reaction(ul)
sterile dH2O34.5
buffer for Taq (-MgCl2)5
10 mM dNTPs1
MgCl22
F primer(#436)2.5
R primer(#436)2.5
Taq0.5
DNA template2
  • Thermocycling
SegmentCyclesTemperatureTime
1195°C1 minute
23095°C30 seconds
50°C1 min
72°C1 min
3172°C7 minutes
4-4°Cinfinite

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