Team:Bologna/Notebook

From 2008.igem.org

(Difference between revisions)
Line 25: Line 25:
* [[Team:Bologna/Protocols#Electrophoretic_run|Electrophoretic run]]
* [[Team:Bologna/Protocols#Electrophoretic_run|Electrophoretic run]]
* [[Team:Bologna/Protocols#Gel_extraction|Gel extraction]]
* [[Team:Bologna/Protocols#Gel_extraction|Gel extraction]]
-
 
+
* [[Team:Bologna/Protocols#Ligation_reaction|Ligation reaction]]
 +
* [[Team:Bologna/Protocols#Chemiocompetent_cells|Chemiocompetent cells]]
 +
* [[Team:Bologna/Protocols#Mediums_and_buffers|Mediums and buffers]]
 +
* [[Team:Bologna/Protocols#Antibiotics_stocks_preparation|Antibiotics stocks preparation]]
 +
* [[Team:Bologna/Protocols#IPTG_stocks_preparation|IPTG stocks preparation]]
== WEEK 1: from 07/21/08 to 07/27/08 ==
== WEEK 1: from 07/21/08 to 07/27/08 ==

Revision as of 08:55, 6 August 2008

Testata sx.jpg Testata dx.jpg
HOME THE PROJECT THE TEAM PARTS SUBMITTED TO THE REGISTRY MODELING NOTEBOOK


Under notebook are all relevant information regarding the work done every week in the laboratory and protocols followed.

Contents

PROTOCOLS

WEEK 1: from 07/21/08 to 07/27/08

  1. Enzymatic digestion of: C0051 E\S, LACI* X\P.
  2. Gel preparation , electrophoretic run.
  3. Amplification of: TETR , PUV, PRECA.


WEEK 2: from 07/28/08 to 08/03/08

  1. Transformation of 2008 registry plasmides: I714930 e I714927 (ceppo TOP10)
  2. Ligation of: R0051 + S0100-T ; J04500 + C0051; R0082 + E0240


WEEK 3: from 08/04/08 to 08/10/08