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Team:Bologna/Notebook
From 2008.igem.org
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Revision as of 15:11, 8 August 2008
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| HOME | THE PROJECT | THE TEAM | PARTS SUBMITTED TO THE REGISTRY | MODELING | NOTEBOOK |
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Under notebook are all relevant information regarding the work done every week in the laboratory and protocols followed.
Contents |
PROTOCOLS
- Plates preparation
- Amplification
- Transformation
- Inoculation
- Miniprep
- Digestion reaction
- Gel preparation
- Electrophoretic run
- Gel extraction
- Ligation reaction
- Chemiocompetent cells
- Mediums and buffers
- Antibiotics stocks preparation
- IPTG stocks preparation
- Fluorescence test
- M9 supplemented media
WEEK 1: from 07/21/08 to 07/27/08
- Enzymatic digestion of: C0051 E\S, LACI* X\P.
- Gel preparation , electrophoretic run.
- Amplification of: TETR , PUV, PRECA.
WEEK 2: from 07/28/08 to 08/03/08
- Transformation of 2008 registry plasmides: I714930 e I714927 (ceppo TOP10)
- Ligation of: R0051 + S0100-T ; J04500 + C0051; R0082 + E0240
WEEK 3: from 08/04/08 to 08/10/08
