User:University of Washington/22 August 2008

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(LuxR from AraC and TetR(Faifan))
 
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==LuxR from pLac==
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-Ligation prodouct of I0462 and R0010 was transformed into DH5a+LacIq cells, and plated on amp.
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==LuxR from AraC and TetR(Faifan)==
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-Got few colonies for LuxR construction, 1 for GFP
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-Re-inoculated the culture from transformation left over that was stored in the fridge from yesterday(Thurs, 8/21/8).
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*concentrate the culture by spinning down, taking 700 ul liquid out(150 ul left), re-suspending the palates and inoculating that on Kan plates.
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*Let them grow at room temp.
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==MG1655Z1(Faifan)==
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-minipreped and ran gel
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*5 ul DNA: Appeared the band still!
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*25 ul DNA: Didn't stay down the well. =[
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-Re-streaked the culture selected from culture#3 Tsy plate on Amp, Kan, Cam, and Tet plates. Let them grow at room temp.
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Back to [[Team:University_of_Washington/Notebook#Notebook]]
Back to [[Team:University_of_Washington/Notebook#Notebook]]

Latest revision as of 21:51, 22 August 2008

LuxR from pLac

-Ligation prodouct of I0462 and R0010 was transformed into DH5a+LacIq cells, and plated on amp.

LuxR from AraC and TetR(Faifan)

-Got few colonies for LuxR construction, 1 for GFP

-Re-inoculated the culture from transformation left over that was stored in the fridge from yesterday(Thurs, 8/21/8).

  • concentrate the culture by spinning down, taking 700 ul liquid out(150 ul left), re-suspending the palates and inoculating that on Kan plates.
  • Let them grow at room temp.

MG1655Z1(Faifan)

-minipreped and ran gel

  • 5 ul DNA: Appeared the band still!
  • 25 ul DNA: Didn't stay down the well. =[

-Re-streaked the culture selected from culture#3 Tsy plate on Amp, Kan, Cam, and Tet plates. Let them grow at room temp.



Back to Team:University_of_Washington/Notebook#Notebook