Team:Chiba/jk/γ

From 2008.igem.org

(Difference between revisions)
Line 73: Line 73:
<br>TRANSFORMATION
<br>TRANSFORMATION
<br>BBa_J63001(yeast optimized YFP)-->colony
<br>BBa_J63001(yeast optimized YFP)-->colony
 +
*'''2008,8,20(wed)'''
 +
Luciferase(J52008:2007,2008 I712019:2008),Lac Z(J33202:2007,2008),Venus(J32007:2007,2008),Craig Venus,T9002,F2620
 +
:結果:成功→Luciferase(J52008:2007),Lac Z(J33202:2007),Craig Venus,T9002,F2620
 +
::失敗→Luciferase(J52008:2008 J52008:2008),Lac Z(J33202:2008),Venus(J32007:2007,2008)
 +
*'''2008,8,21(thu)'''
 +
Lux CDABE(J32007:2007),RBS(B0034:2007)
 +
:結果:成功→RBS
 +
::失敗→Lux CDABE(二回行ったがどちらもコロニーができなかった)→三回目に成功
 +
*'''2008,8,22(sun)'''
 +
Venus YFP
 +
:結果
-
== [[PCR]] ==
 
-
== [[Transformation]] ==
 
== [[Mini prep]] ==
== [[Mini prep]] ==

Revision as of 06:21, 14 September 2008

17,August 2008
PCR
BBa_E0430①
BBa_E0420②
BBa_J06702③
BBa_S01416④
BBa_I73003⑤
BBa_I73004⑥
BBa_I730026⑦
BBa_I763002⑧

>
Sample No
DNA tamplate 11111111
FW primer 55555555
VR primer 55555555
dNTPmix 1010101010101010
thermo pol buffer 1010101010101010
DNA pol(VENT) 11111111
dH2O 6868686868686868
TOTAL 100100100100100100100100

20,August 2008
TRANSFORMATION
BBa_J52008(Luciferase Renilla)-->colony
BBa_I712019(Luciferase Fire fly)-->no colony
BBa_J332002(LacZ)-->colony
BBa_E2030(Venus YFP)-->not so good
YFP(P-GEX)-->colony
BBa_F2620-->colony
BBa_T9002-->colony


21,August 2008
TRANSFORMATION
BBa_J32007(Lux CDABE)-->no colony
BBa_B0034(RBS)-->colony

22,August 2008
MINI PREP
BBa_J52008
BBa_F2620
BBa_T9002
24,August 2008
TRANSFORMATION
BBa_J63001(yeast optimized YFP)-->colony

  • 2008,8,20(wed)

Luciferase(J52008:2007,2008 I712019:2008),Lac Z(J33202:2007,2008),Venus(J32007:2007,2008),Craig Venus,T9002,F2620

結果:成功→Luciferase(J52008:2007),Lac Z(J33202:2007),Craig Venus,T9002,F2620
失敗→Luciferase(J52008:2008 J52008:2008),Lac Z(J33202:2008),Venus(J32007:2007,2008)
  • 2008,8,21(thu)

Lux CDABE(J32007:2007),RBS(B0034:2007)

結果:成功→RBS
失敗→Lux CDABE(二回行ったがどちらもコロニーができなかった)→三回目に成功
  • 2008,8,22(sun)

Venus YFP

結果


Mini prep

AHL receiver

IPTG receiver

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