Team:Bologna/Notebook
From 2008.igem.org
(Difference between revisions)
Line 32: | Line 32: | ||
| '''Safety level''' | | '''Safety level''' | ||
|- align="center" | |- align="center" | ||
- | | | + | | [[Team:Bologna/Protocols#Plates_preparation|Plates preparation]] |
| 1 | | 1 | ||
|} | |} |
Revision as of 13:43, 9 October 2008
HOME | THE PROJECT | THE TEAM | PARTS SUBMITTED TO THE REGISTRY | MODELING | NOTEBOOK | BIOSAFETY |
---|
Under notebook are all relevant information regarding the work done every week in the laboratory and protocols followed.
Contents |
Protocols
Protocol | Safety level |
Plates preparation | 1 |
- Transformation
- Inoculation
- Miniprep
- Digestion reaction
- Gel preparation
- Electrophoretic run
- Gel extraction
- Ligation reaction
- Chemiocompetent cells
- Mediums and buffers
- Antibiotics stocks preparation
- IPTG stocks preparation
- Fluorescence test
- M9 supplemented media
Week 1: from 07/21/08 to 07/27/08
- Enzymatic digestion of: C0051 E\S, LACI* X\P.
- Gel preparation , electrophoretic run.
- Amplification of: TETR , PUV, PRECA.
Week 2: from 07/28/08 to 08/03/08
- Transformation of 2008 registry plasmides: I714930 e I714927 (ceppo TOP10)
- Ligation of: R0051 + S0100-T ; J04500 + C0051; R0082 + E0240
Week 3: from 08/04/08 to 08/10/08