Team:NTU-Singapore/Notebook/24 July 2008

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(New page: =Thursday 24 July= *RESULT from Wednesday ligation and transformation: **E7-Term got 7 colonies!! **Lysis-standard vector and LsrA-standard vector got lots of colonies!! **Lysis-Term also...)
 
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=Thursday 24 July=
=Thursday 24 July=
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*RESULT from Wednesday ligation and transformation:
*RESULT from Wednesday ligation and transformation:
**E7-Term got 7 colonies!!
**E7-Term got 7 colonies!!
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*Digestional gel check for Lysis-Terminator (with Lysis and Term. for comparision) again (this time, we used 2% gel and 100bp ladder). We tried digesting for 1 hour, and 2 hours.<br>'''RESULT:'''
*Digestional gel check for Lysis-Terminator (with Lysis and Term. for comparision) again (this time, we used 2% gel and 100bp ladder). We tried digesting for 1 hour, and 2 hours.<br>'''RESULT:'''
**Digesting for 1 hour and 2 hour didn't make much differences.
**Digesting for 1 hour and 2 hour didn't make much differences.
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**All 3 Lysis-Term bands were actually just Term. So all the 3 colonies we obtained just contained the uncut Terminator.<br>'''NOTE:'''
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***For 2% gel, we should run for at least 1 hour in order to see clear and distinct bands.
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***Terminator vector is 3kb. (as contain both AmpR and KanR), not 2kb as presumed.
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***Bluescript vector is 2.5kb.
*Inoculation: of the colonies we obtained this morning.
*Inoculation: of the colonies we obtained this morning.
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Latest revision as of 02:33, 28 October 2008


Thursday 24 July

  • RESULT from Wednesday ligation and transformation:
    • E7-Term got 7 colonies!!
    • Lysis-standard vector and LsrA-standard vector got lots of colonies!!
    • Lysis-Term also got 5 colonies!!

Hung,Darius:

  • Digestional gel check for Lysis-Terminator (with Lysis and Term. for comparision) again (this time, we used 2% gel and 100bp ladder). We tried digesting for 1 hour, and 2 hours.
    RESULT:
    • Digesting for 1 hour and 2 hour didn't make much differences.
    • All 3 Lysis-Term bands were actually just Term. So all the 3 colonies we obtained just contained the uncut Terminator.
      NOTE:
      • For 2% gel, we should run for at least 1 hour in order to see clear and distinct bands.
      • Terminator vector is 3kb. (as contain both AmpR and KanR), not 2kb as presumed.
      • Bluescript vector is 2.5kb.
  • Inoculation: of the colonies we obtained this morning.
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