Team:University of Sheffield

From 2008.igem.org

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[[Image:UniShefBanner.jpg|center]]
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This is a template page. READ THESE INSTRUCTIONS.
 
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
 
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook.  PLEASE keep all of your pages within your Team:Example namespace. 
 
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{| style="color:#888888;background-color:##888888;" cellpadding="5" cellspacing="2" border="2" bordercolor=#888888 width="85%" align="center"
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!align="center"|[[Team:University_of_Sheffield |Introduction]]
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!align="center"|[[Team:University_of_Sheffield /Project|Our project]]
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!align="center"|[[Team:University_of_Sheffield /Modelling|Modelling]]
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!align="center"|[[Team:University_of_Sheffield /Wet Lab|Wet Lab]]
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!align="center"|[[Team:University_of_Sheffield /Lab Books| Our team]]
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!align="center"|[[Team:University_of_Sheffield /Timetable| Timetable]]
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!align="center"|[[Team:University_of_Sheffield /Misc| Miscellaneous]]
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__NOTOC__  <!-- No table of contents please, this is our homepage. -->
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=On a Mission for Fluorescence...=
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[[image:GFP_structure.jpg|250px|thumb|right|Structure of GFP courtesy of [http://www.tsienlab.ucsd.edu Tsien Lab]]]
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:Example_logo.png|200px|right|frame]]
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|align="center"|[[Team:University_of_Sheffield | Team Example 2]]
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<!--- The Mission, Experiments --->
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Hygiene related diseases have an undeniably huge impact on the world we live in, where many deaths are preventable if sanitary conditions could be improved. This issue is not limited to developing countries: outbursts of diseases caused by water contamination still occur all around the world including the most developed regions. Efficient testing of water is an essential part of preventing these incidents, especially after natural disasters. It needs to be quick, easy enough for anyone to use, cheap and reusable. This was the idea behind the University of Sheffield’s project in its first entry into iGEM.
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By manipulating a pathogen's quorum sensing system and hijacking a pathway already in E.coli, we wanted to build an E.coli cell that could detect ''Vibrio cholerae'' and produce a GFP glow. This would provide the criteria for a good water tester, and hopefully a springboard into the development of other pathogen-detecting E.coli.
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!align="center"|[[Team:University_of_Sheffield|Home]]
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Adding further depth to the project, our sensor was to be focused around the building of a fusion receptor protein. This would consist of the ‘sensing part’ of the pathogen’s receptor (which detects its own quorum signals) fused to the response regulator of the chosen E.coli receptor. GFP fused downstream of the E.coli receptor’s target genes mean that pathogen present = glow!
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!align="center"|[[Team:University_of_Sheffield/Team|The Team]]
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!align="center"|[[Team:University_of_Sheffield/Project|The Project]]
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You can read more about our project [[Team:University_of_Sheffield_/Project | here]], or use the navigation bar above.
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!align="center"|[[Team:University_of_Sheffield/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:University_of_Sheffield/Modeling|Modeling]]
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=Sheffield University=
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!align="center"|[[Team:University_of_Sheffield/Notebook|Notebook]]
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[http://www.shef.ac.uk/ The University of Sheffield] is one of the top 'Russel Group' universities in Great Britain, with very highly ranked  Molecular Biology and Biotechnology and Engineering departments - which is handy for iGEM!   Alternatively, if you fancy a visit, we are home to the best Students Union in the country!
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[[Image:Firth_court.png|center|250px|Firth Court, Molecular Biology and Biotechnology department]]
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(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')
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=Sponsors=
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[[Image:ChELSI_Logo_small.png|thumb|left|100x100px|[http://smic.group.shef.ac.uk/04b_ChELSI.html ChELSI]]]
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[[Image:Prof_Robert_Poole_logo.png|thumb|left|100x100px|[http://www.shef.ac.uk/mbb/staff/poole/poolelab.html Professor Robert Poole]]]
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[[Image:Idtdna-logogif.gif|thumb|left|100x100px|[http://eu.idtdna.com/Home/Home.aspx IDT DNA]]]
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[[Image:Biofusion_logo.png|thumb|left|100x100px|[http://www.fusionip.co.uk/ Fusion IP]]]
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[[Image:IChemE.jpg‎|thumb|left|100x100px|[http://www.icheme.org/enetwork/MainFrameset.asp?AreaID=171 Institute of Chemical Engineering]]]
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[[Image:Sheffield_union_logo.PNG|thumb|left|100x100px|[http://www.shef.ac.uk/union/ Sheffield University Union of Students]]]

Latest revision as of 14:35, 29 October 2008

UniShefBanner.jpg


Introduction Our project Modelling Wet Lab Our team Timetable Miscellaneous


On a Mission for Fluorescence...

Structure of GFP courtesy of [http://www.tsienlab.ucsd.edu Tsien Lab]

Hygiene related diseases have an undeniably huge impact on the world we live in, where many deaths are preventable if sanitary conditions could be improved. This issue is not limited to developing countries: outbursts of diseases caused by water contamination still occur all around the world including the most developed regions. Efficient testing of water is an essential part of preventing these incidents, especially after natural disasters. It needs to be quick, easy enough for anyone to use, cheap and reusable. This was the idea behind the University of Sheffield’s project in its first entry into iGEM.

By manipulating a pathogen's quorum sensing system and hijacking a pathway already in E.coli, we wanted to build an E.coli cell that could detect Vibrio cholerae and produce a GFP glow. This would provide the criteria for a good water tester, and hopefully a springboard into the development of other pathogen-detecting E.coli. Adding further depth to the project, our sensor was to be focused around the building of a fusion receptor protein. This would consist of the ‘sensing part’ of the pathogen’s receptor (which detects its own quorum signals) fused to the response regulator of the chosen E.coli receptor. GFP fused downstream of the E.coli receptor’s target genes mean that pathogen present = glow!

You can read more about our project here, or use the navigation bar above.

Sheffield University

[http://www.shef.ac.uk/ The University of Sheffield] is one of the top 'Russel Group' universities in Great Britain, with very highly ranked Molecular Biology and Biotechnology and Engineering departments - which is handy for iGEM! Alternatively, if you fancy a visit, we are home to the best Students Union in the country!

Firth Court, Molecular Biology and Biotechnology department

Sponsors

[http://smic.group.shef.ac.uk/04b_ChELSI.html ChELSI]
[http://www.shef.ac.uk/mbb/staff/poole/poolelab.html Professor Robert Poole]
[http://eu.idtdna.com/Home/Home.aspx IDT DNA]
[http://www.fusionip.co.uk/ Fusion IP]
[http://www.icheme.org/enetwork/MainFrameset.asp?AreaID=171 Institute of Chemical Engineering]
[http://www.shef.ac.uk/union/ Sheffield University Union of Students]