Virginia/13 August 2008

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*Digested I715039 with E,S and B1002 with E,X to create RFP + term ligation
*Digested I715039 with E,S and B1002 with E,X to create RFP + term ligation
*Cooled off butanolysis apparatus, will do extraction soon
*Cooled off butanolysis apparatus, will do extraction soon
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**Extracted with 3ml MilliQ water. Saved top organic layer in vials labeled 'butanolysis product'
*Bought 1ml (5 * 0.2ml) of Library Efficiency DH5a cells (Invitrogen)
*Bought 1ml (5 * 0.2ml) of Library Efficiency DH5a cells (Invitrogen)
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Latest revision as of 19:45, 13 August 2008

  • Made small broth of A58-A60
    • Not sure about A58 and A59 - few colonies, satellites
  • Made small broth of re-streaked B0034
    • Still not confident in this
  • Digested I715039 with E,S and B1002 with E,X to create RFP + term ligation
  • Cooled off butanolysis apparatus, will do extraction soon
    • Extracted with 3ml MilliQ water. Saved top organic layer in vials labeled 'butanolysis product'
  • Bought 1ml (5 * 0.2ml) of Library Efficiency DH5a cells (Invitrogen)


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