Team:Warsaw/Calendar-Main/10 June 2008

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Latest revision as of 16:59, 12 October 2008


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+<html><h3>Design of primers for fusion of protein A, OmpA and alpha and omega fragments of <br>beta-lactamase </h3><h4>Piotr</h4>
+<p><a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaL_link">alphaL+link14+homo (alphaL_link)</a>
+<br>
+<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaP_XB">alphaP+STOP+XbaI+BamHI (alphaP_XB)</a><br>
+<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaL_link">omegaL+link14+homo (omegaL_link)</a><br>
+<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaP_EPB">omegaP+STOP+EcoRI+PstI+BamHI (omegaP_EPB)</a><br>
+<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpAL+NdeI (ompaL_N)</a> <br>
+<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpAP+link8+SacI+NotI+homo (ompaP_link)</p>
-<p>Plasmid isolation from  colonies.<br>
+</ol>
+</html>
-Control digestion of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.<br>
-Control digestion of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and EcoRI.<br>
-Control digestion of pMPM-T5 + T7 RNA-polymerase with EcoRI.<br>
-Control digestion of pMPM-T5 + T7 RNA-polymerase with BamHI and XhoI.<br>
-DNA gel electrophoresis of digestion products.<br>
-Picking out proper colonies of transformants.<br>
-</p>