Team:PennState/MedalChecklist

Medal Checklist The requirements to earn a Bronze Medal are: #Register the team, have a great summer, and have fun attending the Jamboree. #Successfully complete and submit a Project Summary form. #Create and share a Description of the team's project via the iGEM wiki (see [http://parts.mit.edu/igem07/index.php/Berkeley_UC Berkeley 2007] for a great example). #Present a Poster and Talk at the iGEM Jamboree (watch [http://parts.mit.edu/igem07/jam07media/Jam07_ETHZ.mp4 ETHZ 2007] for a great example). #Enter information detailing at least one new standard BioBrick Part or Device in the Registry of Parts #*Entered information for each new part or device should at least include primary nucleic acid sequence, description of function, authorship, any relevant safety notes, and an acknowledgement of sources and references. Consider [http://parts.mit.edu/registry/index.php/Part:BBa_J45004 BBa_J45004] as one example (be sure to check Main, Design Page, and Experiences sub-pages for this part). #*Teams are currently (as of May 3) expected to design and contribute standard biological parts that conform to the accepted BioBrick standards for physical assembly. Non-BioBrick parts will not be recognized by iGEM 2008 judges. The two specific BioBrick physical assembly schemes that the judges will recognize by default are (i) Tom Knight's [http://dspace.mit.edu/handle/1721.1/21168 original assembly standard] and (ii) Ira Phillips [http://dspace.mit.edu/handle/1721.1/32535 fusion assembly standard]. #**[Special Note. A discussion has been initiated by the BioBricks Standards Working Group to consider updating the BioBrick assembly standard in time for June 1. Check back for any updates on acceptable BioBrick assembly standards.] #*Any new Devices that are based on gene expression are expected to conform to the PoPS device boundary standard. See chapter 3 of the book, [http://openwetware.org/wiki/Adventures Adventures in Synthetic Biology], for more information about common signal carriers and PoPS. #Submit DNA for at least one new BioBrick Part or Device to the Registry of Parts. #*The submitted DNA must be associated with a Part or Device for which you have entered information describing the part or device, and must conform to the BioBrick standards for Parts or Devices (see above). The requirements to earn a Silver Medal, in addition to the Bronze Medal requirements, are: #Demonstrate that at least one new BioBrick Part or Device of your own design and construction works as expected. #Characterize the operation of at least one new BioBrick Part or Device and enter this information on the Parts or Device page via the Registry of Parts (see [http://parts.mit.edu/registry/index.php/Part:BBa_F2620 BBa_F2620] for an exemplar). The requirements to earn a Gold Medal, in addition to the Silver Medal requirements, are any one OR more of the following: #Characterize or improve an existing BioBrick Part or Device that was distributed via the 2008 iGEM BioBrick Parts collection, and enter this information back on the Registry. #Help another iGEM team by, for example, charactering a part, debugging a construct, or modeling or simulating their system. #Develop and document a new technical standard that supports the (i) design of BioBrick Parts or Devices, or (ii) construction of BioBrick Parts or Devices, or (iii) characterization of BioBrick Parts or Devices, or (iv) analysis, modeling, and simulation of BioBrick Parts or Devices, or (v) sharing BioBrick Parts or Devices, either via physical DNA or as information via the internet. #Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.

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-<td valign="top" id="pagecontent" width="80%"><span style="font-size: 16pt">Diauxie Introduction</span>
+<td valign="top" id="pagecontent" width="80%"><span style="font-size: 16pt">Medal Checklist</span>
-<hr/>
-<p class="start">A common framework for gene expression at bacterial catabolic operons involves dual regulation by a global regulatory protein and a catabolite-specific regulator (e.g., AraC in the case of expression from promoter PBAD).  In <em>E. coli</em>, the cAMP-receptor protein (CRP) acts as a global regulator in which the cAMP-CRP complex typically increases transcription at catabolic promoters in the absence of the “preferred” catabolite glucose. The result is a phenomenon known as diauxie, in which glucose is preferentially utilized in the presence of other sugars, since expression of catabolic pathways for the other sugars is not fully induced.  A consequence of this dual control mechanism is that many bacterial promoters commonly used in biotechnology require the absence of glucose for full transcription activation (e.g., the <em>lac</em> and <em>araBAD</em> promoters).</p>
+The requirements to earn a Bronze Medal are:
-<p>In wild-type <em>E. coli</em> strains, the promoters controlling expression of genes responsible for xylose transport and metabolism are regulated by CRP and the xylose-inducible protein XylR. Our goal in this project is to create and characterize a xylose-inducible but glucose-insensitive gene expression system. This would functionally eliminate a diauxie-type phenotype relating to induction of gene expression from this promoter.  In addition to creating a valuable new tool for the part registry, this project has useful applications for biochemical and bioenergy production.  Cellulosic biomass feedstocks targeted for biofuel production or other value-added products contain large percentages of glucose and xylose.  In industrial fermentations, cells grown on sugars from cellulosic biomass normally consume glucose as their first carbon source.  Then, depending on the process, cells either change gene expression to utilize xylose, or the cells and leftover sugars are removed as waste.  Both situations lead to inefficiency in production, especially if a continuous growth process is desired. Growing cells on multiple sugars results in a lag time as the cells switch from glucose to xylose metabolism, which complicates and delays the overall process.  The gene expression system we are creating could aid in the simultaneous fermentation of mixed sugars, and would have practical applications during the conversion of biomass to ethanol, and for other processes using bacteria for fermentation of low-cost sugar mixtures.</p>
+#Register the team, have a great summer, and have fun attending the Jamboree.
+#Successfully complete and submit a Project Summary form.
+#Create and share a Description of the team's project via the iGEM wiki (see [http://parts.mit.edu/igem07/index.php/Berkeley_UC Berkeley 2007] for a great example).
+#Present a Poster and Talk at the iGEM Jamboree (watch [http://parts.mit.edu/igem07/jam07media/Jam07_ETHZ.mp4 ETHZ 2007] for a great example).
+#Enter information detailing at least one new standard BioBrick Part or Device in the Registry of Parts
+#*Entered information for each new part or device should at least include primary nucleic acid sequence, description of function, authorship, any relevant safety notes, and an acknowledgement of sources and references.  Consider [http://parts.mit.edu/registry/index.php/Part:BBa_J45004 BBa_J45004] as one example (be sure to check Main, Design Page, and Experiences sub-pages for this part).
+#*Teams are currently (as of May 3) expected to design and contribute standard biological parts that conform to the accepted BioBrick standards for physical assembly. Non-BioBrick parts will not be recognized by iGEM 2008 judges.  The two specific BioBrick physical assembly schemes that the judges will recognize by default are (i) Tom Knight's [http://dspace.mit.edu/handle/1721.1/21168 original assembly standard] and (ii) Ira Phillips [http://dspace.mit.edu/handle/1721.1/32535 fusion assembly standard].
+#**[Special Note.  A discussion has been initiated by the BioBricks Standards Working Group to consider updating the BioBrick assembly standard in time for June 1.  Check back for any updates on acceptable BioBrick assembly standards.]
+#*Any new Devices that are based on gene expression are expected to conform to the PoPS device boundary standard.  See chapter 3 of the book, [http://openwetware.org/wiki/Adventures Adventures in Synthetic Biology], for more information about common signal carriers and PoPS.
+#Submit DNA for at least one new BioBrick Part or Device to the Registry of Parts.
+#*The submitted DNA must be associated with a Part or Device for which you have entered information describing the part or device, and must conform to the BioBrick standards for Parts or Devices (see above).
+The requirements to earn a Silver Medal, in addition to the Bronze Medal requirements, are:
+#Demonstrate that at least one new BioBrick Part or Device of your own design and construction works as expected.
+#Characterize the operation of at least one new BioBrick Part or Device and enter this information on the Parts or Device page via the Registry of Parts (see [http://parts.mit.edu/registry/index.php/Part:BBa_F2620 BBa_F2620] for an exemplar).
+The requirements to earn a Gold Medal, in addition to the Silver Medal requirements, are any one OR more of the following:
+#Characterize or improve an existing BioBrick Part or Device that was distributed via the 2008 iGEM BioBrick Parts collection, and enter this information back on the Registry.
+#Help another iGEM team by, for example, charactering a part, debugging a construct, or modeling or simulating their system.
+#Develop and document a new technical standard that supports the (i) design of BioBrick Parts or Devices, or (ii) construction of BioBrick Parts or Devices, or (iii) characterization of BioBrick Parts or Devices, or (iv) analysis, modeling, and simulation of BioBrick Parts or Devices, or (v) sharing BioBrick Parts or Devices, either via physical DNA or as information via the internet.
+#Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.