Team:Bologna/Parts

From 2008.igem.org

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Revision as of 09:22, 2 October 2008

Logo1a.gifTestata dx.jpg
HOME THE PROJECT THE TEAM PARTS SUBMITTED TO THE REGISTRY MODELING NOTEBOOK


Intermediates

The table below shows all the intermediates that we have obtained to build up our final device. For each part you can find:

-in the first column the link to the Registry;

-in the second column the components for each part;

-in the third column the plasmid the part has been cloned in;

-in the fourth the insert length after double digestion with Xba1/Pst1 enzymes linked to the electrophoresis gel run;

-in the fifth column cell vitality after transformation;

-in the sixth column fluo data for each part.


Part number Replaced by Description Type Component Status
K145004 K145104 T7 PoPS + RiboLock generate LacI Repressor. LacI Repressor represses transcription of LuxI from pLac. Composite part Inverter Discarded
K145005 / cI under control of a dual promotor and a RiboLock. Composite part Pulse generator Discarded
K145007 K145107 Pulse Generator Composite part (nowhere) Discarded
K145008 K145108 Constitutive production of LuxR Composite part (nowhere) Discarded
K145009 K145109 ccdB cell death gene under control of an activating Lux PR Composite part (nowhere) Discarded
K145010 K145110 Killing switch Composite part (nowhere) Discarded
K145011 / Light sensor to provide dummy input for the system, copy of BBa_M30109 Composite part Input Deleted
K145012 / N-terminal degradation tag Part Filter Not used
K145013 / antisense LuxI Part Memory In use
K145014 / T7 RNA Polymerase with N-terminal UmuD degradation tag Part Filter In use
K145015 / GFP with LVA tag Part Ouput In use
K145016 / Intermediate TetR-generator Composite part Input In use
K145100 K145205 OmpF PoPS -> GFP Composite part Output Discarded
K145101 K145110 OmpF PoPS -> deactivate constitutive antisense LuxI Composite part Memory Discarded
K145102 K145215 OmpF PoPS -> RiboKey3d Composite part Filter Discarded
K145103 K145216 T7 with UmuD derived tag under constitutive promotor + RiboLock3d Composite Part Filter Discarded
K145104 K145220 T7 PoPS + RiboLock -> LacI -> inhibition of LuxI Composite part Inverter Discarded
K145105 K145225 cI under T7 and PR dual promotor Composite part Pulse Generator Discarded
K145106 / cI PRM PoPS -> Lactonase Composite part Pulse Generator Unknown
K145107 K145117 Lactonase pulse generator Composite part Pulse Generator Discarded
K145108 / Constitutive PoPS -> LuxR Composite part Cell death Unknown
K145109 / Hybrid P22 c2 - luxPR PoPS -> ccdB Composite part Cell death Unknown
K145110 / Cell death mechanism Composite part Cell death Unknown
K145111 / OmpF PoPS -> deactivate constitutive antisense LuxI Composite part Memory Unknown
K145112 / cI under T7 and PRR dual promotor Composite part Pulse generator Unknown
K145113 / Constitutive PoPS -> TetR  ?  ? Unknown
K145115 / cI under T7 and PRR dual promotor Composite part Pulse generator Unknown
K145117 / Lactonase pulse generator Composite part Pulse generator Unknown
K145150 / Hybrid promoter: HSL-LuxR activated, P22 C2 repressed Part CellDeath In use
K145151 / ccdB coding region Part CellDeath In use
K145152 / Hybrid promoter: P22 c2 , LacI NOR gate Part  ? Unknown
K145200 / INPUT lightsensor (DISCARDED) Composite Part Input Unknown
K145201 / INPUT TetR generator Composite Part INPUT Unknown
K145205 / OUTPUT GFP under TetR pomoter Composite Part OUTPUT Unknown
K145210 / MEMORY Composite Part MEMORY Unknown
K145211 / MEMORY OUTPUT asLuxI Composite Part MEMORY Unknown
K145215 / FILTER Key Composite Part FILTER Unknown
K145216 / FILTER T7 RNA pol Composite Part FILTER Unknown
K145217 / FILTER Complete Composite Part FILTER Unknown
K145220 / INVERTED TIMER Composite Part INVERTIMER Unknown
K145225 / RESET lactonase Composite Part RESET Unknown
K145230 / CELL DEATH Composite Part CELLDEATH Unknown