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Team:Warsaw/Calendar-Main/19 June 2008
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<h4>Piotr</h4> | <h4>Piotr</h4> | ||
There is no mutation in beta-galactosidase gene. Why the colonys are white??? | There is no mutation in beta-galactosidase gene. Why the colonys are white??? | ||
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| + | <h3> Blue/white test </h3> | ||
| + | <h4>Piotr</h4> | ||
| + | <ol> | ||
| + | <li>Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a>.<br> | ||
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| + | Template: DNA isolated from white colonies<br> | ||
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| + | Primers: <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqL">pZCseqL</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqR">pZCseqR</a><br> | ||
| + | <br> | ||
| + | <table id="result"> | ||
| + | <tr><th colspan="3">Colony PCR program</th></tr> | ||
| + | <tr><th>Temperature</th><th>Time</th><th>No. of cycles</th></tr> | ||
| + | <tr><td>94°C</td><td>4:00</td></tr> | ||
| + | <tr><td>94°C</td><td>0:30</td><td rowspan="3">28 cycles</td></tr> | ||
| + | <tr><td>48°C</td><td>0:45</td></tr> | ||
| + | <tr><td>72°C</td><td>1:30</td></tr> | ||
| + | <tr><td>72°C</td><td>10:00</td></tr> | ||
| + | <tr><td>4°C</td><td>infinite</td></tr> | ||
| + | </table> | ||
| + | <br> | ||
</html> | </html> | ||
Revision as of 18:24, 19 October 2008
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Preparation of constructs with OmpA protein fusionsMichał K.Inoculation of separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin). The analysis of beta-galactosidase sequence from pZCPiotrThere is no mutation in beta-galactosidase gene. Why the colonys are white???Blue/white testPiotr |
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