Team:Warsaw/Calendar-Main/29 May 2008

From 2008.igem.org

(Difference between revisions)
Line 3: Line 3:
<html>
<html>
<h3>Rifampicin test</h3>
<h3>Rifampicin test</h3>
-
<h4>Michał L., Ewa, Marcin</h4>
+
<h4>Michał L., Ewa, Marcin, Piotr</h4>
<p><ol>
<p><ol>
<li> Added 50 μL <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>  carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPM-T5+AID</a> (in liquid LB) into liquid LB.</li>
<li> Added 50 μL <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>  carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPM-T5+AID</a> (in liquid LB) into liquid LB.</li>
Line 17: Line 17:
<li> 1 ml (2x) from p.1, p.2, p.3, p.4 were plated on LB + rifampicin. </li>
<li> 1 ml (2x) from p.1, p.2, p.3, p.4 were plated on LB + rifampicin. </li>
-
</ol></p>
 
-
<h3></h3>
 
-
<h4>Piotr</h4>
 
-
<p>OD measurement of above cultures.</p>
 
 +
 +
<li>OD measurement of above cultures.</li>
 +
</ol></p>
</html>
</html>
<!-- do not remove this! -->
<!-- do not remove this! -->
{{WarNotebookEnd}}
{{WarNotebookEnd}}

Revision as of 14:11, 26 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Rifampicin test

Michał L., Ewa, Marcin, Piotr

  1. Added 50 μL E. coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB.
  2. Added 50 μL E. coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB + 0.02% arabinose.
  3. Added 50 μL E. coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB.
  4. Added 50 μL E. coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB + 1 mM IPTG.
  5. Cultures were incubated for 5 hours.
  6. 1 ml (2x) from p.1, p.2, p.3, p.4 were plated on LB + rifampicin.
  7. OD measurement of above cultures.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/29_May_2008"