Change of the reporter from pZC320 with B-galactosidase to GFP or RFP

Piotr, Weronika, Emilia

1. Gel electrophoresis and gel-out of proper bands.
2. Ligation of pZC320 with standard parts: BBa_E0840(GFP genetrator) and BBa_J04450 (RFP generator).
3. Chemotransformation of E.coli TOP10 with ligation products.
4. Plating transformants on LB+Amp30+X-gal+IPTG.

Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega

Paweł

1. Clean-up of overnight digest.
2. Ligation of OmpA_alpha (from Michał K.) and pET15b.
3. Ligation of OmpA_omega (from Michał K.) and pET15b.
4. Transformation of E. coli TOP10 strain with ligation products.
5. Transformants plating on LB + ampicillin.