Team:Warsaw/Calendar-Main/23 July 2008
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>pACYC177+OmpA_A_alpha</a>). </li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>pACYC177+OmpA_A_alpha</a>). </li> | ||
- | <li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids with BamHI and SacI (BamHI buffer).</li><li> Gel electrophoresis - proper clone found. | + | <li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids with BamHI and SacI (BamHI buffer).</li><li> Gel electrophoresis - proper clone found. <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/23_July_2008#fig1">Fig. 1</a></li></ol> |
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- | <img src="https://static.igem.org/mediawiki/2008/f/fb/23_july.jpg" width=300/> <var><b>Fig. 1. Control SacI/BamHI digests of pACYC177+OmpA_A_alpha</b><br> | + | <a name="fig1><img src="https://static.igem.org/mediawiki/2008/f/fb/23_july.jpg" width=300/></a> <var><b>Fig. 1. Control SacI/BamHI digests of pACYC177+OmpA_A_alpha</b><br> |
1-4. digested plasmids isolated from transformants obtained previous day<br> | 1-4. digested plasmids isolated from transformants obtained previous day<br> |
Revision as of 17:22, 26 October 2008
Cloning omega-A fusion on pKS (second attempt)Michał L., Ewa, Marcin
Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpAPawełLigations transformed into TOP10 and plated on LB + ampicillin. AntoniSetup of overnight culture E. coli TOP10 (LB broth) for preparation of chemocompetent bacteria. Cloning of protein A DNA to OmpA constructsMichał K.
1-4. digested plasmids isolated from transformants obtained previous day 5. Marker
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