Team:Warsaw/Calendar-Main/6 October 2008
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primers on colonies from plates with transformations <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a>+ <a href=http://partsregistry.org/Part:BBa_K103009>linker_alpha (BBa_K103009)</a> (annealing temperature - 55°C,60 s of elongation step). No visible bands for proper product.</li> | primers on colonies from plates with transformations <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a>+ <a href=http://partsregistry.org/Part:BBa_K103009>linker_alpha (BBa_K103009)</a> (annealing temperature - 55°C,60 s of elongation step). No visible bands for proper product.</li> | ||
- | <li>Inoculation of some colonies which grown on plate - <a href=http://partsregistry.org/Part:pSB2K3>pSB2K3</a> + | + | <li>Inoculation of some colonies which grown on plate - <a href=http://partsregistry.org/Part:pSB2K3>pSB2K3</a> + <a href=http://partsregistry.org/Part:BBa_K103009>linker_alpha (BBa_K103009)</a> to liquid LB + kanamycin. </li></ol> |
Revision as of 23:24, 26 October 2008
Preparation of pSB2K3 + _alphaMichał K.
Preparation of pSB2K3 + _omegaMichał K.Inoculation of some colonies which grown on plate - pSB2K3 + _omega to liquid LB + kanamycin. Preparation of BioBricksMichał K.
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