Team:Warsaw/Calendar-Main/6 October 2008
From 2008.igem.org
(Difference between revisions)
MKrzyszton (Talk | contribs) |
|||
Line 38: | Line 38: | ||
- | + | <h3>Preparation of pLac_OmpA_omega</h3> | |
+ | <h4>Michał K.</h4> | ||
Line 46: | Line 47: | ||
<h3>Preparation of BioBricks</h3> | <h3>Preparation of BioBricks</h3> | ||
<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
- | < | + | <p> Overnight <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">ligation</a> of isolated DNA fragments <a href=http://partsregistry.org/Part:pSB2K3>pSB2K3</a> + pLac_OmpA_omega (without EcoRI site).</p> |
Line 57: | Line 58: | ||
- | + | ||
</ol> | </ol> |
Revision as of 10:37, 27 October 2008
Preparation of linker_alpha (BBa_K103009)Michał K.
Preparation of linker_omega (BBa_K103013)Michał K.Inoculation of some colonies which grown on plate - pSB2K3 + linker_omega (BBa_K103013) to liquid LB + kanamycin. Preparation of OmpA-linker-omega-linker (BBa_K103016)Michał K.
Preparation of vector for pT7 constructsMichał K.Overnight selfligation of pET15b+OmpA_omega without XbaI. Preparation of pLac_OmpA_omegaMichał K.Preparation of BioBricksMichał K.Overnight ligation of isolated DNA fragments pSB2K3 + pLac_OmpA_omega (without EcoRI site).
|