User:University of Washington/2 July 2008
From 2008.igem.org
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- The remaining purified plasmid solutions were stored at -20 degrees Celsius. | - The remaining purified plasmid solutions were stored at -20 degrees Celsius. | ||
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+ | |||
+ | == LuxR from pLac == | ||
+ | |||
+ | - I0462 transformed cells failed to grow on Amp plate. I0462 has now failed to transform twice. | ||
+ | |||
+ | - Part I763004 was transformed into DH strain, in substition for defective part J04430. | ||
[[Team:University_of_Washington/Notebook#Notebook]] | [[Team:University_of_Washington/Notebook#Notebook]] |
Revision as of 23:05, 2 July 2008
BioBrick Promoter Measurements
- The four overnight cultures of TOP10 cells containing promoter constructs I20260, I20268, I20269, and I20270 were centrifuged at 14,000 RPM for 3 minutes. Then, the supernatant was decanted out, leaving only pelleted cells.
- The pelleted cells were miniprepped using a Qiagen miniprep kit.
- 4 uL of each plasmid sequence was added to its own two tubes. One tube had 8 uL of forward primer added to it, and the other had 8 uL of reverse primer added to it. These reaction tubes were submitted to the UW Sequencing Facility for sequencing.
- The remaining purified plasmid solutions were stored at -20 degrees Celsius.
LuxR from pLac
- I0462 transformed cells failed to grow on Amp plate. I0462 has now failed to transform twice.
- Part I763004 was transformed into DH strain, in substition for defective part J04430.
Team:University_of_Washington/Notebook#Notebook