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User:University of Washington/10 July 2008
From 2008.igem.org
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== non-RP4 Conjugation == | == non-RP4 Conjugation == | ||
| - | '''Mini Prep'''<br\> | + | '''Mini Prep & [[UW Glycerol Stocks]]'''<br\> |
·CV13(Yep13)<br\> | ·CV13(Yep13)<br\> | ||
·pDPT51 | ·pDPT51 | ||
Revision as of 00:20, 10 July 2008
RP4 Conjugation
Conjugation protocol #1
·DH5α
·DH5α + S17-1 w/ RP4
·DH5α + naked RP4 DNA
·DH5α + amp resistant RFP plasmid
Lambda Red
·Synthesis tetR insert for KorA and trbA replacement
non-RP4 Conjugation
Mini Prep & UW Glycerol Stocks
·CV13(Yep13)
·pDPT51
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