"
User:University of Washington/9 July 2008
From 2008.igem.org
(Difference between revisions)
| Line 14: | Line 14: | ||
- Overnight promoter-construct cultures were diluted 1:100, then incubated in a rotator for 3 hours. | - Overnight promoter-construct cultures were diluted 1:100, then incubated in a rotator for 3 hours. | ||
| - | - | + | - Using a 96-well plate reader, fluorescence and OD600 measurements were taken every five minutes for eight hours for the four promoter-construct cultures, an empty culture of TOP10 cells, M9 media, and M9+Kan. |
Revision as of 19:00, 11 July 2008
RP4 Conjugation
·Success!!! Bacteria-Bacteria conjugation works
·Received lambda red primers for RP4 manipulation
LuxR from AraC and TetR
- Took AraC plasmid after thermo cycling out from the machine, stored in the fridge.
- Plated GFP generator (BBa_E0240: received from BioCircuit Lab) on LB Agar + Amp.
BioBrick Promoter Measurements
- Overnight promoter-construct cultures were diluted 1:100, then incubated in a rotator for 3 hours.
- Using a 96-well plate reader, fluorescence and OD600 measurements were taken every five minutes for eight hours for the four promoter-construct cultures, an empty culture of TOP10 cells, M9 media, and M9+Kan.
Back to Team:University_of_Washington/Notebook#Notebook
