Team:Johns Hopkins/Notebook
From 2008.igem.org
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Part no.: BBa_K110011 | Part no.: BBa_K110011 | ||
Part Description: Between-bud 27-W FRS2-C LtR | Part Description: Between-bud 27-W FRS2-C LtR | ||
- | Part Location (in build a genome lab): In James and Jasper's PCR product Box, Stainless Steel 4 degree | + | Part Location (in build a genome lab): In James and Jasper's PCR product Box, |
+ | Stainless Steel 4 degree | ||
PCR successful?; Yes | PCR successful?; Yes | ||
- | Cloning of PCR product successful: Yes (will come soon; I can put it in the wiki to make it easier for you) | + | Cloning of PCR product successful: Yes (will come soon; I can put it |
+ | in the wiki to make it easier for you) | ||
Sequencing of cloned PCR product successful: No | Sequencing of cloned PCR product successful: No | ||
Joining of validated part to adjacent part(s) status: Not done | Joining of validated part to adjacent part(s) status: Not done | ||
Problems to be solved: | Problems to be solved: | ||
- | Current status of this part: Miniprep of Overnight cultures will be completed today | + | Current status of this part: Miniprep of Overnight |
+ | cultures will be completed today | ||
status report by: James | status report by: James | ||
Part no.: BBa_K110012 | Part no.: BBa_K110012 | ||
Part Description: Between STE2-W and BST1-C LtR | Part Description: Between STE2-W and BST1-C LtR | ||
- | Part Location (in build a genome lab): In James and Jasper's PCR product Box, Stainless Steel 4 degree | + | Part Location (in build a genome lab): In James and Jasper's PCR product Box, |
+ | Stainless Steel 4 degree | ||
PCR successful?; Yes | PCR successful?; Yes | ||
- | Cloning of PCR product successful: Yes (will come soon; I can put it in the wiki to make it easier for you) | + | Cloning of PCR product successful: Yes (will come soon; I can put it in the |
+ | wiki to make it easier for you) | ||
Sequencing of cloned PCR product successful: No | Sequencing of cloned PCR product successful: No | ||
Joining of validated part to adjacent part(s) status: Not done | Joining of validated part to adjacent part(s) status: Not done | ||
Problems to be solved: | Problems to be solved: | ||
- | Current status of this part: Miniprep of Overnight cultures will be completed today | + | Current status of this part: Miniprep of Overnight cultures will be |
+ | completed today | ||
status report by: James | status report by: James | ||
Part no.: BBa_K110013 | Part no.: BBa_K110013 | ||
Part Description: Between-SWP82-W and EMP47-C LtR | Part Description: Between-SWP82-W and EMP47-C LtR | ||
- | Part Location (in build a genome lab): In James and Jasper's PCR product Box, Stainless Steel 4 degree | + | Part Location (in build a genome lab): In James and Jasper's PCR product Box, |
+ | Stainless Steel 4 degree | ||
PCR successful?; No | PCR successful?; No | ||
- | Cloning of PCR product successful: No (will come soon; I can put it in the wiki to make it easier for you) | + | Cloning of PCR product successful: No (will come soon; I can put it in the wiki |
+ | to make it easier for you) | ||
Sequencing of cloned PCR product successful: No | Sequencing of cloned PCR product successful: No | ||
Joining of validated part to adjacent part(s) status: Not done | Joining of validated part to adjacent part(s) status: Not done |
Revision as of 16:42, 12 July 2008
Notebook
Contents |
Files
iGEM Groups V1.0
iGEM Groups V2.01
Important reminders and notes
[Can make general comments here, so they don't get lost in peoples e-mail boxes]
July-11: Primers for group 1 were delivered yesterday
July 11: Lab meeting at 7:30PM in the lab to go over miniprep protocol
Tuesday July 15: Lab meeting at 6:30PM with Jessica. Have status reports ready.
Status Reports
GROUP 1: Fluorescent Proteins
status report by: Ingrid (work done by James) Part no.: BBa_K110017 Part Description: yESapphire RtL Part Location (in build a genome lab): In James and Jasper's PCR product Box, Stainless Steel 4 degree PCR successful?; Yes Cloning of PCR product successful: Y/N Sequencing of cloned PCR product successful: Not done Joining of validated part to adjacent part(s) status: Not done Problems to be solved: The PCR of this part yielded a very large product Current status of this part:
status report by: Ingrid (work done by James) Part no.: BBa_K110010 Part Description: yESapphire LtR Part Location (in build a genome lab): In James and Jasper's PCR product Box, Stainless Steel 4 degree PCR successful?; Yes Cloning of PCR product successful: Not done Sequencing of cloned PCR product successful: No Joining of validated part to adjacent part(s) status: Not done Problems to be solved: The PCR of this part yielded a very large product Current status of this part:
GROUP 2: MATa Specific-promoters
Status report by Allison and Nate Part no.: BBa_K110008 Part Description: MFA1 (L+R) Part Location: in a labeled box, second shelf from the top, -20 degrees C refrigerator next to front door Date: 7/10/08 PCR successful? Yes Cloning of PCR product successful: in progress Sequencing of cloned PCR product successful: not done Joining of validated part to adjacent part(s) status: not done Problems to be solved: to be determined Current status of this part: PCR was being troubleshooted, appeared to have good results with regular PCR protocol (not touchdown) in which there was a constant annealing temperature of 55 degrees C - see gel
Status report by Allison and Nate Part no.: BBa_K110016 Part Description: Ste2 (R+L) Part Location: in a labeled box, second shelf from the top, -20 degrees C refrigerator next to front door Date: 7/10/08 PCR successful? Yes Cloning of PCR product successful: in progress Sequencing of cloned PCR product successful: not done Joining of validated part to adjacent part(s) status: not done Problems to be solved: to be determined Current status of this part: Both PCR protocols (touchdown and second PCR with constant annealing temperature) produced product of the correct size. BBa_K110016 was used as a control in the second PCR with BBa_K110008.
GROUP 3: Short two way stops
Date: 7/11/08 status report by: James Part no.: BBa_K110011 Part Description: Between-bud 27-W FRS2-C LtR Part Location (in build a genome lab): In James and Jasper's PCR product Box, Stainless Steel 4 degree PCR successful?; Yes Cloning of PCR product successful: Yes (will come soon; I can put it in the wiki to make it easier for you) Sequencing of cloned PCR product successful: No Joining of validated part to adjacent part(s) status: Not done Problems to be solved: Current status of this part: Miniprep of Overnight cultures will be completed today
status report by: James Part no.: BBa_K110012 Part Description: Between STE2-W and BST1-C LtR Part Location (in build a genome lab): In James and Jasper's PCR product Box, Stainless Steel 4 degree PCR successful?; Yes Cloning of PCR product successful: Yes (will come soon; I can put it in the wiki to make it easier for you) Sequencing of cloned PCR product successful: No Joining of validated part to adjacent part(s) status: Not done Problems to be solved: Current status of this part: Miniprep of Overnight cultures will be completed today status report by: James Part no.: BBa_K110013 Part Description: Between-SWP82-W and EMP47-C LtR Part Location (in build a genome lab): In James and Jasper's PCR product Box, Stainless Steel 4 degree PCR successful?; No Cloning of PCR product successful: No (will come soon; I can put it in the wiki to make it easier for you) Sequencing of cloned PCR product successful: No Joining of validated part to adjacent part(s) status: Not done Problems to be solved: The PCR of this part yielded a very large product Current status of this part:
GROUP 4
GROUP 5
etc....